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Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples

BMC Infectious Diseases volume 21, Article number: 125 (2021) Cite this article

Abstract

Background

Streptococcus agalacticae (Group B Streptococcus, GBS) is one of the most important causative agents of serious infections among neonates. This study was carried out to identify antibiotic resistance and virulence genes associated with GBS isolated from pregnant women.

Methods

A total of 43 GBS isolates were obtained from 420 vaginal samples collected from HIV positive and negative women who were 13–35 weeks pregnant attending Antenatal Care at Chitungwiza and Harare Central Hospitals in Zimbabwe. Identification tests of GBS isolates was done using standard bacteriological methods and molecular identification testing. Antibiotic susceptibility testing was done using the modified Kirby-Bauer method and E-test strips. The boiling method was used to extract DNA and Polymerase Chain Reaction (PCR) was used to screen for 13 genes. Data was fed into SPSS 24.0.

Results

Nine distinct virulence gene profiles were identified and hly-scpB-bca-rib 37.2% (16/43) was common. The virulence genes identified were namely hly 97.8% (42/43), scpB 90.1% (39/43), bca 86.0% (37/43), rib 69.8% (30/43) and bac 11.6% (5/43). High resistance to tetracycline 97.7% (42/43) was reported followed by 72.1% (31/43) cefazolin, 69.8% (30/43) penicillin G, 58.1% (25/43) ampicillin, 55.8% (24/43) clindamycin, 46.5% (20/43) ceftriaxone, 34.9% (15/43) chloramphenicol, and 30.2% (13/43) for both erythromycin and vancomycin using disk diffusion. Antibiotic resistance genes among the resistant and intermediate-resistant isolates showed high frequencies for tetM 97.6% (41/42) and low frequencies for ermB 34.5% (10/29), ermTR 10.3% (3/29), mefA 3.4% (1/29), tetO 2.4% (1/42) and linB 0% (0/35). The atr housekeeping gene yielded 100% (43/43) positive results, whilst the mobile genetic element IS1548 yielded 9.3% (4/43).

Conclusion

The study showed high prevalence of hly, scpB, bca and rib virulence genes in S. agalactiae strains isolated from pregnant women. Tetracycline resistance was predominantly caused by the tetM gene, whilst macrolide resistance was predominantly due to the presence of erm methylase, with the ermB gene being more prevalent. Multi-drug resistance coupled with the recovery of resistant isolates to antimicrobial agents such as penicillins indicates the importance of GBS surveillance and susceptibility tests. It was also observed that in vitro phenotypic resistance is not always accurately predicted by resistance genotypes.

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Background

Streptococcus agalactiae (Group B Streptococcus; GBS) is the leading cause of neonatal infections and serious infections in pregnant women in many countries [1]. GBS generally colonizes asymptomatically as a member of the gastrointestinal or vaginal microbiota but can cause life-threatening infections [2, 3]. About 15 to 35% of pregnant women are colonized vaginally, and approximately 1 to 2% of colonized neonates develop a severe infection [4]. About 25% of pregnant women have S. agalactiae in their rectum or vagina [1] which can be passed via vertical transmission to the unborn baby leading to early onset disease in the form of sepsis, pneumonia and meningitis [4].

In low income countries such as Zimbabwe, pregnant women hardly get access to GBS diagnostic tests and antibiotics are not generally affordable for the general public. A study done in three communities in Zimbabwe showed a cumulative 60.3% GBS colonization rate of women who were tested 3 times and that same study showed that GBS colonization is common among pregnant women in the country [5].

S. agalactiae uses virulence factors (VFs) encoded by its genes to enter, replicate and persist in a host [6]. Antimicrobial resistance genes confer GBS the ability to resist the effects of medication and these genes are found in the environment [7]. The increasing emergence of antibiotic resistant S. agalactiae clearly raises concern for sustained measures in treatment of such life-threatening bacterial infection [8].

The virulence factors in GBS include the capsular polysaccharide antigens (CPA) which is used for strain typing [9, 10]. In Zimbabwe, CPA serotyping and nucleic acid based methods have been used to characterize GBS antigens [11, 12]. Other genes that have been studied in other countries include those for the alpha/α antigens of the C protein (bca), beta/β antigens of the C protein (bac), surface protein Rib (rib), C5a peptidase (scpB) and hyaluronate lyase (hly) [6, 9, 13]. To our knowledge only one study [11] in this country investigated Rib, Cα and Cβ protein markers in GBS. However, data on some of the other known virulence genes is not yet available.

Penicillin and ampicillin are the drugs of choice for treatment of human S. agalactiae infections [14,15,16] whereas for penicillin-allergic individuals, erythromycin and clindamycin are recommended [1]. Generally, GBS is regarded to be 100% susceptible to penicillin and ampillicin [14, 17, 18]. However, some studies in Ethiopia, USA and Japan have reported clinical isolates exhibiting intermediate and reduced susceptibility to both penicillin G and ampicillin [19,20,21,22,23]. The prevalence of resistance to erythromycin, tetracycline and clindamycin has recently been increasing in S. agalactiae [14, 15, 24]. Various researchers have linked this resistance to specific genes found in GBS [14, 16, 25].

Molecular tests are necessary when monitoring the spread of resistant organisms and their genes throughout the communities and medical facilities such as hospitals and clinics [26]. Such tests are important if we are to control the increase in resistance as well as the emergence of multi-drug resistant bacteria. A good example being the study of mobile genetic elements (MGEs) which are a type of DNA that can move around and within the genome [27]. Strategies to fight off bacterial infections by targeting specific virulence genes and mobile genetic elements have been suggested [28].

A total of 13 genes were screened in GBS including 5 virulence genes hyaluronate lyase, hly; beta/β antigens of the C protein (bac); alpha/α antigens of the C protein (bca); surface protein Rib, rib [9] and C5a peptidase, scpB [29]. The 6 antibiotic resistance genes investigated are the tetracycline resistance determinants tetM and tetO [25]; erythromycin ribosomal methylase determinants ermB and ermTR; Macrolide efflux mefA and clindamycin resistance determinant linB [14]. The Mobile Genetic Element IS1548 [9] and the housekeeping gene atr [30] was also amplified. The atr gene was selected for analysis in the current study because it is an excellent target for S. agalactiae identification. It is an essential gene which has been well studied and has a low probability of mutation [30].

GBS virulence and antibiotic resistance gene studies are important in the development of vaccines and in understanding the resistance mechanisms used by pathogens, respectively. The aim of this study was to identify virulence factors and antibiotic resistance genes found in GBS isolates from pregnant women clinical samples as well as to study the phenotypic antimicrobial resistance and the correlation between the different genes found in GBS isolates.

Methods

Study design and sample collection

A cross-sectional study was carried out enrolling 420 healthy pregnant women of 35–38 gestational age on their scheduled Antenatal Care (ANC) routine visit at the Harare and Chitungwiza Central hospitals in Zimbabwe. Using a consecutive sampling method two vaginal swabs were collected during October 2016 to November 2016 period from which the 43 GBS isolates were obtained. Consenting participants were administered with a questionnaire to obtain demographic data, syphilis and HIV status. This was performed by trained research nurses.

Inclusion and exclusion criteria

Participants who were on treatment for any STI and those who had received antibiotic treatment 1 month prior to recruitment were excluded from the study. All pregnant women who did not give their consent as well as those who were less than 13 weeks pregnant and less than 18 years of age were also excluded from the study.

Sample size determination

Sample size was calculated using a single proportion estimate with specified precision. A two sided significance level alpha was set at 5%, power of 80% and a prevalence of GBS in Zimbabwe of 31.6% according to Moyo et al., (2000) [31]. The sample size was based on an estimated proportion of 0.33, confidence level of 0.95 and a desired precision of estimate of 0.005. The estimated sample size became 340 plus 5% attrition giving a total minimum sample size of 357.

Isolation and identification of Streptococcus agalactiae

A total of 43 suspected GBS isolates were obtained from the vaginal swabs. These isolates were subcultured by streaking onto 5% sheep blood agar plate (Columbia CNA Agar) (Acumedia, USA). The plates were incubated at 37 °C for 18–24 h in aerobic conditions. The suspected β-hemolytic colonies were identified as GBS using standard microbiological S. agalactiae identification tests such as the Gram stain (gram positive cocci), the Catalase test (catalase negative), Bile Aesculin test, CAMP (Christie, Atkins, Munch, Petersen) test which were confirmed using the Streptex grouping latex reagent (Remel, UK). The isolates identified as GBS strains were then stored in a cation-adjusted Mueller Hinton broth along with 20% glycerol at − 80 °C. The negative culture results were issued after 72 h.

Antimicrobial susceptibility testing

The following 9 antimicrobial discs (Oxoid, Basingstoke, UK) and concentrations were selected: penicillin G (10 units), ampicillin (10 μg), vancomycin (30 μg), cefazolin (30 μg), ceftriaxone (30 μg), chloramphenicol (30 μg), tetracyline (30 μg), clindamycin (2 μg) and erythromycin (15 μg). Antimicrobial susceptibility testing (AST) of GBS was done on 5% sheep blood containing Mueller-Hinton agar using the modified Kirby Bauer methods as recommended by the Clinical Laboratory Standard Institute (CLSI 2017) [32]. The medium was poured into 90 mm diameter sterile petri dishes to a depth of 4 mm (about 25 ml per plate). This was done on a level surface so that the depth of the medium is uniform. Bacterial inocula were prepared by suspending 3–4 freshly grown GBS colonies in 3–5 ml sterile physiological saline and turbidity was adjusted to 0.5 McFarland standard used as a reference to adjust the bacterial suspension. A sterile cotton swab was used to inoculate and excess fluid was removed by pressing and rotating the swab against the side of the tube above the level of the suspension. It was then swabbed over the entire surface of the agar. Then, water-soluble antibiotic impregnated paper disks were placed on the plate and incubated in 5% CO2 at 37 °C for 24 h. Incubated, plates were examined to ensure the growth was confluent or near confluent before zone diameters were measured. The minimum inhibitory concentrations (MICs) of ampicillin, chloramphenicol and vancomycin were also confirmed using E-test strips (BioMerieux, Basingstoke, UK). The MICs for three antimicrobials were defined as the lowest concentration of antibiotic that completely inhibited bacterial growth. The susceptibilities of GBS to antibiotics were categorized as sensitive, intermediate and resistant based on the CLSI 2017 guidelines [32].

Quality control

Streptococcus pneumoniae (ATCC 49619), Streptococcus agalactiae (ATCC 12386), Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 29213) were used as quality control in this study.

DNA isolation and quantification

After subculturing the stored isolates, DNA was extracted using thermal lysis (the boiling method). A pipette was used to place 600 μl of sterile Tris-EDTA (TE) buffer pH 8.0 (Amresco, USA) into a 1.5 ml sterile Eppendorf tube. A sterilized loop was used to emulsify the cultured colonies in TE and this mixture was vortexed for 30–45 s before placing the tightly closed Eppendorf tubes into a boiling water bath for 15 min. A − 20 °C freezer was then used to freeze the boiled mixture for 10 min. DNA samples were stored at − 20 °C in Tris-EDTA buffer at pH 8.0 to minimize the degradation, before being tested for quality and quantity. The preparation of × 1 Tris-Borate electrophoresis (TBE) buffer, 1% agarose gel and molecular weight markers (1 kb and 50 bp) were all done according to manufacturer’s instructions.

The PCR process was done in four stages, i.e. amplification of the housekeeping gene atr, the mobile genetic element IS1548, the antibiotic resistance genes (ermB, ermTR, mefA, linB, tetM and tetO) and the virulence genes (hly, bca, bac, rib and scpB).

PCR reactions mixtures and conditions

Amplification of all the genes was done using standard PCR. For each DNA sample a 25 μl reaction mixture was made which consisted of 15.875 μl of nuclease-free water (Amresco, USA), 2.5 μl of × 10 PCR buffer (New England BioLabs, UK), 0.5 μl of dNTPs Solution Mix, 10 mM (New England BioLabs, UK), 0.125 μl Taq DNA polymerase, 5 U/ μl (New England BioLabs, UK), 5 μl of DNA template and 1 μl of the 10 μM primer (0.5 μl of forward primer and 0.5 μl of reverse primer) (Inqaba biotech, South Africa). The primers used in our study are listed (see Additional file 2: Table S1). It should be noted that for each PCR test, DNA samples of clinical isolates were simultaneously proliferated and evaluated with a positive control sample (DNA sample of S. agalactiae, ATCC 12386) and a negative control (Nuclease free water). A Labnet Multigene OptiMax (Labnet International Inc., USA) was used for the PCR thermal cycling conditions with an initial denaturation step at 94 °C for 1 min, 35 cycles {denaturation 94 °C for 1 min, annealing at 55 °C for 1 min, extension 72 °C for 1 min} and a final elongation step at 72 °C for 10 min. Ten microliters of the amplified products were mixed with 5 μl of gel loading dye (6×) then run along a 1% ethidium bromide (Amresco, USA) (EtBr final conc. of 0.5 μl/ml) stained agarose gel with a 1 kb and 50 bp DNA ladder (New England BioLabs, UK) in 1× TBE buffer (Amresco, USA) for 1 h 30 mins at 100 V (Biobase, China) and then viewed using a Wealtec KETA UV Transilluminator (Wealtec Corp, USA).

The contents of each 25 μl reaction mixture is summarized in Table 1. Optimization of the PCRs was performed by adjusting the DNA template concentration and the annealing time.

Table 1 Final primer concentrations and volumes used in the PCRs
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Statistical analysis

All data entry and analysis was done using IBM Statistical Package for the Social Sciences (SPSS) version 24.0 software. Prevalence figures were calculated for the total sample population. Tests for normality were done such as the Shapiro-Wilks test and Skewness-Kurtosis All Normality Test. P value less than 0.05 was considered statistically significant.

Sample characteristics

Tetracyline resistance genes

A Shapiro-Wilks test (p < 0.05) and a visual inspection of their histograms, normal Q-Q plots and box plots showed that tetracycline resistance was not normally distributed for tetM and tetO with a skewness of 6.481 (S.E = 0.365) and a kurtosis of 42 (S.E = 6.717) for both tetM and tetO.

Erythromycin resistance genes

A Shapiro-Wilks test (p < 0.05) and a visual inspection of their histograms, normal Q-Q plots and box plots showed that erythromycin resistance was not normally distributed for ermTR, mefA and ermB, with a skewness of 2 (S.E = 1.014) and a kurtosis of 4 (S.E = 2.679) for ermTR, a skewness of 0.816 (S.E = 0.369) and a kurtosis of − 1.405 (S.E = 0.724) for mefA and a skewness of 0.321 (S.E = 0.409) and a kurtosis of − 2.023 (S.E = 0.793) for ermB.

Clindamycin resistance and the linB gene could not be tested for normality because linB’s absence was constant and that showed a complete absence of correlation which is represented by 0. The VFs could not also be tested for normality, because they did not have an established dependent and independent variable, however a correlation did exist. Since the data that was tested is not normal, we used non-parametric test, such as the Spearman rank correlation test (see Additional file 2: Table S5). A p-value of 0.05 was used for determining statistical significance.

Results

Participant characteristics

A total of 420 Zimbabwean pregnant women between 13 and 35 weeks of gestation with a median age of 29 years were recruited and sampled in the study. Of these 10.2% (43/420) were vaginally colonized by GBS and of the 43 women, 95.3% (41/43) were HIV-uninfected and 4.7% (2/43) were HIV-infected.

Antibiotic susceptibility profiles

The 43 GBS isolates in Table 2 suggest a 97.6% (42/43) resistance of GBS to tetracycline, 72.1% (31/43) cefazolin, 69.8% (30/43) penicillin G, 58.1% (25/43) ampicillin, 55.8% (24/43) clindamycin, 46.5% (20/43) ceftriaxone, 34.9% (15/43) chloramphenicol, and 30.2% (13/43) for both erythromycin and vancomycin.

Table 2 Antibiotic susceptibility profiles of 43 GBS isolates from pregnant women
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Multi-drug resistance pattern

Multidrug resistance (MDR) (≥ 3 drug classes) was detected in 67.4% (29/43) of the isolates. Resistance to 5, 6, 7, 8 and 9 drugs was found to be 34.2%, 17.0%, 23.9%, 20.6% and 3.4% respectively as shown in Table 3.

Table 3 Multi-drug resistance pattern of GBS isolated from pregnant women recruited from two health institutions (n = 43)
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Virulence gene profiles

Nine distinct virulence gene profiles were identified and the virulence gene profiles hly-scpB-bca-rib 37.2% (16/43) and hly-scpB-bca 18.6% (8/43) were common among GBS isolates as shown in Table 4.

Table 4 Virulence gene profiles of the 43 Streptococcus agalactiae isolates
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Prevalence of virulence genes and antibiotic resistance genes

Table 5 shows the percentages of all the genes being investigated (see Additional file 2: Table S2). The housekeeping gene atr was present in all 43 isolates, whilst the MGE IS1548 was present in only 4 of the isolates. The virulence gene hly had the highest frequency followed by scpB, bca, rib but bac which was found in a few isolates. The VFs frequencies were calculated for the whole GBS population (43 isolates). The antibiotic resistance gene tetM had a high percentage when compared with tetO. The ermB determinant was moderately present followed by ermTR and mefA which was rarely found. The linB gene was not detected in any of the isolates. The percentage of the resistant determinants was obtained by combining only the intermediate and resistant populations (I + R) (see Additional file 2: Table S3).

Table 5 Frequency of all the 13 genes in the 43 GBS isolates
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Discussion

Our study is the first prevalence report of detection of some of the GBS virulence genes and antibiotic resistance genes in pregnant women in Zimbabwe. The 10.2% prevalence of maternal GBS colonization reported is similar to the 12.2% and 13.4% reported in Ethiopia [19] and Saudi Arabia [33], respectively. However, its lower when compared to the 2010 study in Zimbabwe [5] and this could be due to the effectiveness of some of the ongoing awareness campaigns on pregnant women’s health and early testing. Other possible reasons for these discrepancies are differences in the time of sampling, specimen, sample sizes, geography, detection techniques and gestational ages among other factors.

The atr gene and the Mobile genetic element

The sensitivity of the atr gene amplification, in this study was 100% (43/43) (Table 5) which is similar to what is reported in other studies done by Gavino and Wang [34], de-Paris et al., [30] and Alfa et al. [35], where sensitivity was 95.8%, 100% and 90.5% respectively. This high sensitivity may be attributed to the use of selective and enriched media prior to performing PCR. Since this PCR test has shown to be reliable and robust, future studies can focus on rapidly testing pregnant women who present in labor with unknown GBS status. This test was used as a GBS confirmatory identification test. The Mobile Genetic Element IS1548 was present in only 9.3% (4/43) (Table 5). This finding is contrary to an Iranian study which showed that the gene is more prevalent in human than in bovine (77% vs 5%) GBS isolates [9]. The origin of the isolates could be a possible explanation for such a difference.

Virulence genes

Nine distinct virulence gene profiles were observed but 30/43 strains (69.8%) belonged to hly-scpB-bca-rib 37.2% (16/43), hly-scpB-bca 18.6% (8/43) or hly-scpB-rib 14.0% (6/43) profiles (Table 3). The PCR assay for detection of 'VFs revealed that high percentage of the GBS isolates were positive for hly 97.8% (42/43); scpB 90.1% (39/43) and bca 86.0% (37/43) (Table 5). The high prevalence of these genes in GBS has been previously reported [9, 36,37,38].

A Zimbabwean study reported a 46.3% of Rib (rib) protein marker as well as 19.8% and 15.7% of Cα (bca) and Cβ (bac) protein markers, respectively [11]. The low frequency obtained for the β protein marker agrees with the 11.6% for bac observed in this study and this can be attributed to the reported low distribution of the Ib and II CPA types (11.6% and 8.3%, respectively) in the Zimbabwean GBS population [11]. However, contrary to their findings, the present study reports higher prevalence of rib (69.8%) and bca (86.0%). This discrepancy could be attributed to differences in CPA types for GBS isolates in these two studies among other factors. Comparisons with this 2008 study, highlighted the importance of investigating the CPA of GBS isolates because a relationship was observed between protein markers and CPA types [11].

Lower frequencies for genes rib and bca, are reported by Manning et al., (2006) and Persson et al., (2008) with 28% and 43% for rib and 29% and 14% for bca in the USA [39] and Sweden [40] respectively. However, our higher frequencies agree with the 76.1% for rib and 88.6% for bca reported in Argentina [41]. The bac gene in the present study was found at a similar frequency to the aforementioned studies in USA and Sweden: 11.6% for bac, compared with 20% and 12% for bac, in the USA and Sweden respectively. The study in Argentina observed a higher frequency of 52.3% for bac. A 2017 study in Egypt also observed high frequencies for scpB in 100% and rib in 79.2% and a lower frequency for bac (35.8%) in GBS isolates. However, none of this study’s isolates possessed the bca gene [29]. Our comparison with studies from these four countries USA, Sweden, Argentina and Egypt shows that any discrepancies are possibly a result of the geographical locations among other factors.

The results also showed that the majority of the isolates had more than three virulence genes. This high prevalence of various VFs in S. agalactiae isolates from the vaginal canal of pregnant women could lead to the development of serious maternal and neonatal infections [42]. However, this study was not able to follow up on the outcomes of both the pregnant mothers and their newborns. The high incidences of VFs except for bac, also suggest that GBS vaccines containing the proteins ScpB, α protein [43], Rib [11] and hyaluronate lyase [44] could potentially be effective against our population of pregnant women in Zimbabwe.

The present study observed that all the isolates 100% (n = 5) carrying the bac gene also carry the unrelated bca gene, whilst 86.5% (n = 32) of the bca carrying isolates did not also have the bac gene (see Additional file 2: Table S2). This finding agrees with the report that, isolates that express β tend to also express the unrelated α protein, while the α protein is often expressed on its own in GBS isolates [45, 46]. The majority of the Spearman rank analysis were not statistically significant and with some showing weak associations. However, a significant negative correlation (see Additional file 2: Table S5) was detected between hly and IS1548 as well as between bac and rib genes (P < 0.01). This negative association is most likely because these genes are not genetically linked.

Multi-drug resistance

Multi-drug resistance was shown in 67.4% (29/43) of the isolates with 21 patterns identified (Table 3). Our MDR value is much higher than the 43.9% reported in Ethiopia [21] and yet lower than the 90% reported in GBS in China [47]. Differences in MDR frequencies could be due to use of different antimicrobials, MDR definitions and AST methods. The high resistance observed using disk diffusion method was confirmed by our comparatively higher MIC50s and MIC90s (Table 2) for ampicillin, vancomycin and chloramphenicol to those reported in a GBS study in Japan [48]. The lowest resistance in our study was observed in 30.2% (13/43) of the GBS isolates to both vancomycin and erythromycin (Table 2). Lower resistance of 17%, 7.5% and 0% to vancomycin, erythromycin and chloramphenicol respectively has been reported by Assefa et al. [21] and other researchers [20, 33]. Higher resistance to chloramphenicol (45%) in GBS isolates has been reported in Iran [15]. Our results showed a 34.9% (15/43) resistance to chloramphenicol using disk diffusion method and 0% (0/43) resistance using the E-test strips (MICs, ≥16 μg/ml). This is because 93.5% (29/31) isolates which had tested intermediate or resistant to chloramphenicol by disk diffusion, were determined to be susceptible by E-test strips (see Additional file 2: Table S4). Both the disk diffusion and E-test results vancomycin showed similar results except for three isolates.

A high resistance to penicillin G 69.8% (30/43) and ampicillin 58.1% (25/43) (disk diffusion method) and 46.5% (20/43) (E-test strips) was observed. This is because five isolates had resistance to ampicillin by disk diffusion but were determined to be susceptible by E-test strips (MICs, ≤0.25 μg/ml). The high resistance is contrary to most studies which report a uniform susceptibility of 100% to all GBS isolates in pregnant women [14, 18, 33, 49, 50]. However, our results agree with recent findings in Ethiopia were a resistance of 77.3% to penicillin G was reported in pregnant women [19]. Other studies have reported resistance to both penicillin G and ampicillin of 10.2% and 9.2% as well as 19.5% and 14.6% respectively [20, 21]. The high resistance reported may be due to the wide and non-prescription use of these two drugs in our study area [49, 51]. It may also be as a result of differences in laboratory procedures or bacterial strains. This study also reports resistance to other penicillin surrogates [52] such as ceftriaxone 46.5% (20/43) and cefazolin 72.1% (31/43). A similar observation of approximately 31% for ceftriaxone was reported [20, 53], whilst a lower range of 0% -15% for cefazolin has been reported [53,54,55]. We recommend that antibiotic susceptibility testing should be performed if penicillin G or ampicillin therapy is needed in the prevention of neonatal GBS infection. Further studies could focus on identifying the genes responsible for the observed resistance.

Tetracyline resistance

The high resistance for GBS to tetracycline 97.7% (42/43) (Table 2) can be attributed to a high presence of the tetM gene 97.6% (41/42) (see Additional file 1: Fig. 9). This high tetM gene presence is as a result of the ubiquitous presence of tet genes in pathogens, opportunistic pathogens and members of the normal flora [56]. The high resistance to tetracycline is also because the antibiotic is a relatively cheap, extensively used prophylaxis in the therapy of animal and human infections. It is known that bacterial strains tend to be resistant to frequently used antibiotics [51]. A low presence rate of the tetO 2.4% (1/42) (Table 5) indicates that this gene is not common in GBS isolates that are resistant to tetracycline. These results agree with studies done in Canada [57] and Nigeria [58]. In Kuwait, they also reported similar results with 89.5% tetracycline-resistant isolates carrying 94.5% tetM and 3.9% tetO genes [59].

Erythromycin and clindamycin resistance

The current study results showed a 30.2% (13/43) and 55.8% (24/43) resistance to erythromycin and clindamycin respectively (Table 2). Such resistance decreases the options of prophylaxis in penicillin allergic women [60]. This combined with the high resistance of GBS to penicillin and ampicillin, we recommend that AST should be performed if clindamycin or erythromycin therapy is needed in the prevention of neonatal GBS infection. Future studies should focus on finding suitable alternative antibiotics for GBS chemoprophylaxis in pregnant women. PCR detected 34.5% (10/29) ermB, 10.3% (3/29) ermTR and 3.4% (1/29) mefA from the intermediate and resistant erythromycin GBS (Table 5). The prevalence of the ermB determinant shows that GBS commonly use target methylation as the mechanism of macrolide resistance.

Studies done in Italy [61], South Africa [14], USA [16], Iran [15] and France [25] reported similar findings, with most of the studies also observing that the ermB gene is more prevalent in distribution than the ermTR gene among GBS strains. This study also confirmed findings by Poyart et al., (2003) that the mefA gene is rare among GBS isolates, thus efflux pumps mediated by this gene are not a common mechanism of macrolide resistance [25]. Contrary to Bolukaoto et al., (2015) this current study did not find any linB genes 0% (0/35) in any of the clindamycin resistant and intermediate strains [14]. In such cases, where multiple independent resistance genes can cause resistance, the observed phenotypic resistance can be attributed to any of the other known genes or genes that are yet to be discovered. This however limits the usefulness of such diagnostic tests.

It was interesting to note that 63.6% (7/11) GBS which were resistant to both erythromycin and clindamycin had the ermB gene (see Additional file 2: Table S2 and S3) and that one GBS strain which was resistant to erythromycin and susceptible to clindamycin carried the ermTR gene. Such observations are similar to other studies [16, 62, 63].

Genetic linkage in macrolide/tetracycline resistance determinant

It was also observed that 100% (n = 10) isolates that carried the ermB gene also carried the tetM gene. The association of erythromycin and tetracycline resistance may be due to the conjugative transposon Tn1545, which encodes erythromycin resistance via the ermB gene and tetracycline resistance via the tetM gene [64,65,66]. This association was supported by our analysis which showed a positive correlation (see Additional file 2: Table S5) between the two genes, but this lacked statistical significance according to the Spearman Rank test. Contrary to an Egyptian study [67], we report that there is no evidence suggesting genetic linkage of tetO with ermB, ermTR or mefA in our GBS population.

Unexpressed resistant genotypes

An unexpected observation in our study was found in the following three (7.0%) isolates. Isolate 127 which was tetracycline sensitive, and yet the tetM gene was detected. Isolates 243 and 322, were both erythromycin sensitive however, at least one gene ermTR or mefA was found, respectively (see Additional file 2: Table S2 and S3). Similar findings were reported in GBS and other streptococcal species [66, 68,69,70]. However, based on the disk diffusion test, this observation suggests that the Kirby Bauer disk diffusion method is inadequate for detection of resistance. Although the reason(s) behind this lack of gene expression still has to be determined, some possible explanations include gene mutations, absence of selection pressure (i.e. presence of antibiotic), low expression levels of the gene and the possibility of a weak, distant or absent promoter [66, 71, 72]. This study therefore supports the idea that the resistance genotype does not always accurately predict phenotypic resistance.

Conclusion

An increased resistance against penicillin G, ampicillin, tetracycline, erythromycin, vancomycin and clindamycin among other drugs was observed. The antibiotics erythromycin and vancomycin can be reliable alternatives in treating or preventing GBS infection in women who suffer from colonization or participants that are allergic to penicillin in Harare and Chitungwiza. However, prescribing antibiotic without antibacterial susceptibility tests should be avoided because of the high prevalence of resistance. The tetracycline resistance encoded by ribosome protection gene tetM and the ribosomal methylation encoded by erythromycin ribosomal methylase gene, ermB were the two most common mechanisms of resistance in GBS from pregnant women. Current study results also showed that resistance genotypes do not always accurately predict phenotypic resistance.

A high frequency of the virulence genes: hly, scpB, bca and rib was detected in GBS and the majority of the isolates carried multiple virulence genes which showed that GBS’s ability to adhere, colonize, destroy and invade tissues is important in the organism’s pathogenesis. The bac gene and the Mobile Genetic Element (IS1548) were rarely found among the GBS isolates from pregnant women.

Limitations of the study

It would have been necessary to test each isolate using an in vivo animal model, such as mice to demonstrate actual virulence and investigate the expression of resistant genes. A small number of isolates was included from the two centres, which might have caused some bias. Our disc-diffusion assay had no replicates, and this is statistically a weak point. Also pipetting a fixed volume of inoculum for each assay plate is a better method of controlling the volume of inoculum used. In future studies it may be useful to collect information on co-morbidities in participants whose analysis would be related to the various VFs identified.

Availability of data and materials

All data generated or analysed during this study are included in this published article [and its supplementary information files].

Abbreviations

GBS:

Group B Streptococcus

VF:

virulence factors

CDC:

Centers for Disease Control

WHO:

World Health Organization

CPA:

capsular polysaccharide antigens

MGE:

mobile genetic elements

MLSB:

Macrolides, Lincosamides, and Streptogramin B

ANC:

Antenatal Care

PCR:

Polymerase Chain Reaction

AST:

Antimicrobial susceptibility testing

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Acknowledgements

The authors would like to express their gratitude to the members of the Department of Medical Microbiology and the Department of Chemical Pathology (University of Zimbabwe). We would like extend our great appreciation to Dr. L R. Mazengera and the staff of Immuno-Path Diagnostic Laboratory Services, the National Microbiology Reference Laboratory (NMRL) and the Department of Medicine (University of Zimbabwe) for their facilities and equipment. We extend our gratitude to Harare and Chitungwiza central hospitals ANC staff for helping during recruitment, and our heartfelt gratitude to ANC mothers who consented to participate in our study. We also acknowledge Letten Foundation for financial support for sample collection. We wish to thank and acknowledge the support given by Taona Mudhluli.

Funding

Letten Foundation funded the sample collection. The funders had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, National Polio Laboratory, University of Zimbabwe College of Health Sciences, P. O. Box A178, Avondale, Harare, Zimbabwe

    Raymond Mudzana & Rooyen T. Mavenyengwa

  2. Faculty of Health Sciences, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Room No. 3.22 Falmouth Building, Anzio Road, Observatory, Cape Town, 7925, South Africa

    Muchaneta Gudza-Mugabe

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Contributions

RM, RTM, MGM: conceived the idea and contributed to the study design. MGM: Cohort recruitment, sample collection and completed the antibiotic resistance testing. RM: designed and performed the molecular testing. RTM: designed and supervised the project. RM, RTM: analyzed data and interpreted the results. RM, RTM, MGM: wrote manuscript. All authors read and approved the final paper for publication.

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Correspondence to Raymond Mudzana.

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Ethics approval and consent to participate

This study obtained approvals from the institutions involved namely Harare Central Hospital (HCHEC 080416/21); Chitungwiza Central Hospital ethics committees (dated 28/04/16) which approved sample collection from their hospital antenatal clinics and the Joint Research Ethics Committee (JREC/341/17) for the College of Health Sciences and Parirenyatwa Group of Hospitals. The study was also approved by Medical Research Council of Zimbabwe (MRCZ A/2054) and (MRCZ/B/1403). All information of participants was kept confidential. Written informed consent from the study participants was sought and granted by the participants. All information and data regarding of the participants was kept confidential and it was only accessible to personnel involved in the study. All electronic data was password protected.

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Not Applicable.

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The authors declare that they have no competing interests.

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Supplementary Information

Additional file 1.

Gel Electrophoresis Pictograms (Fig. 1 to Fig. 9).

Additional file 2:

Five supplementary tables; Table S1. The oligonucleotide primers used for amplifying various genes in GBS. Table S2. Presence or absence of the expected amplicons in the 43 GBS isolates. Table S3. Presence or absence of antibiotic resistance genes in the resistant and intermediate isolates. Table S4. Disk diffusion zone diameters and E-test results for the 43 GBS isolates. Table S5. GBS Virulence and Antibiotic Resistance Genes Spearman Rank Correlations.

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Mudzana, R., Mavenyengwa, R.T. & Gudza-Mugabe, M. Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples. BMC Infect Dis 21, 125 (2021). https://doi.org/10.1186/s12879-021-05820-6

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Keywords

  • Group B streptococcus
  • Virulence genes
  • Antibiotic resistance genes
  • PCR
  • Zimbabwe

BMC Infectious Diseases

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