Table 1 Final primer concentrations and volumes used in the PCRs
Components | 25 μl Reaction | Final Conc. |
|---|---|---|
10× PCR Standard Taq reaction buffer | 2.5 μl | 1× |
10 mM dNTPs Solution Mix | 0.5 μl | 200 μM |
10 μM Forward Primer | 0.5 μl | 0.2 μM |
10 μM Reverse Primer | 0.5 μl | 0.2 μM |
Template GBS DNA | 5 μl | variable |
Taq DNA Polymerase | 0.125 μl | 1.25 U/50 μl PCR |
Nuclease Free Water | 15.875 μl | – |