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Table 1 Final primer concentrations and volumes used in the PCRs

From: Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples

Components 25 μl Reaction Final Conc.
10× PCR Standard Taq reaction buffer 2.5 μl
10 mM dNTPs Solution Mix 0.5 μl 200 μM
10 μM Forward Primer 0.5 μl 0.2 μM
10 μM Reverse Primer 0.5 μl 0.2 μM
Template GBS DNA 5 μl variable
Taq DNA Polymerase 0.125 μl 1.25 U/50 μl PCR
Nuclease Free Water 15.875 μl