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Diagnostic accuracy of tests to detect Hepatitis C antibody: a meta-analysis and review of the literature
BMC Infectious Diseases volume 17, Article number: 695 (2017)
Abstract
Background
Although direct-acting antivirals can achieve sustained virological response rates greater than 90% in Hepatitis C Virus (HCV) infected persons, at present the majority of HCV-infected individuals remain undiagnosed and therefore untreated. While there are a wide range of HCV serological tests available, there is a lack of formal assessment of their diagnostic performance. We undertook a systematic review and meta-analysis to evaluate he diagnostic accuracy of available rapid diagnostic tests (RDT) and laboratory based EIA assays in detecting antibodies to HCV.
Methods
We used the PRISMA checklist and Cochrane guidance to develop our search protocol. The search strategy was registered in PROSPERO (CRD42015023567). The search focused on hepatitis C, diagnostic tests, and diagnostic accuracy within eight databases (MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, Science Citation Index Expanded, Conference Proceedings Citation Index-Science, SCOPUS, Literatura Latino-Americana e do Caribe em Ciências da Saúde and WHO Global Index Medicus. Studies were included if they evaluated an assay to determine the sensitivity and specificity of HCV antibody (HCV Ab) in humans. Two reviewers independently extracted data and performed a quality assessment of the studies using the QUADAS tool. We pooled test estimates using the DerSimonian-Laird method, by using the software R and RevMan. 5.3.
Results
A total of 52 studies were identified that included 52,673 unique test measurements. Based on five studies, the pooled sensitivity and specificity of HCV Ab rapid diagnostic tests (RDTs) were 98% (95% CI 98-100%) and 100% (95% CI 100-100%) compared to an enzyme immunoassay (EIA) reference standard. High HCV Ab RDTs sensitivity and specificity were observed across screening populations (general population, high risk populations, and hospital patients) using different reference standards (EIA, nucleic acid testing, immunoblot). There were insufficient studies to undertake subanalyses based on HIV co-infection. Oral HCV Ab RDTs also had excellent sensitivity and specificity compared to blood reference tests, respectively at 94% (95% CI 93-96%) and 100% (95% CI 100-100%). Among studies that assessed individual oral RDTs, the eight studies revealed that OraQuick ADVANCE® had a slightly higher sensitivity (98%, 95% CI 97-98%) compared to the other oral brands (pooled sensitivity: 88%, 95% CI 84-92%).
Conclusions
RDTs, including oral tests, have excellent sensitivity and specificity compared to laboratory-based methods for HCV antibody detection across a wide range of settings. Oral HCV Ab RDTs had good sensitivity and specificity compared to blood reference standards.
Background
Hepatitis C is a liver disease caused by the hepatitis C virus (HCV) that causes acute and chronic infection [1, 2]. An estimated 71 million people had chronic hepatitis C infection worldwide in 2015 [3]. Viral hepatitis caused 1.34 million deaths in 2015, a number comparable to deaths caused by tuberculosis and higher than those caused by HIV [3]. The introduction of direct-acting antivirals (DAAs) has led to a sustained virological response (SVR) in greater than 90% of treated individuals [4, 5]. DAAs are now recommended by the World Health Organization (WHO) [1] and many other HCV treatment guidelines [1]. DAAs will not only improve SVR rates but also may simplify HCV management algorithms and allow smaller health facilities to manage HCV-infected individuals [6]. Despite the availability of effective treatment, most HCV-infected individuals remain undiagnosed and untreated [7]. Left untreated, approximately 15–30% of individuals with chronic HCV infection progress to cirrhosis, leading to end-stage liver disease and hepatocellular carcinoma [1, 2].
In February 2016 the WHO updated the guidelines for the screening, care, and treatment of persons with chronic hepatitis C infection [1]. These guidelines included recommendations on whom to screen for HCV and how to confirm HCV infection, but not which tests are optimal for initial screening. Advances in HCV detection technology create new opportunities for enhancing screening, referral, and treatment. Previous systematic reviews on HCV infection have focused on treatment response [8, 9], clinical complications [10], and epidemiology [11, 12]. Two previous systematic reviews on hepatitis C testing have focused on evaluating point-of-care tests compared to EIAs and other reference tests [13, 14]. We have undertaken a further systematic review and meta-analysis to generate pooled sensitivity and specificity of rapid diagnostic tests used to detect HCV antibody (HCV Ab), and to inform the development of recommendations on serological testing in the 2017 WHO testing guidelines [15].
Methods
Research question
The main purpose of the review was to assess the diagnostic accuracy of available assays for detecting HCV Ab in persons identified for hepatitis C testing. The research question was structured in a PICO format (ie. population, intervention, comparisons and outcome).
P: Persons identified for HCV testing; I: Rapid diagnostic tests and enzyme immunoassays for HCV Ab detection; C: 1), EIA (with a subanalysis based on the last 10 years); 2), NAT (nucleic acid testing); 3), Immunoblot or similar assay; 4), A combination of 1,2,3 above; O: Diagnostic accuracy [Sensitivity (SE), Specificity (SP), Positive predictive value (PPV), Negative predictive value (NPV), True Negative, True Positive (TP), False negative (FN), and False positive (FP)].
Search strategy and identification of studies
Search strategies were developed by a medical librarian with expertise in designing systematic review searches. Our search algorithm consisted of the following components: hepatitis C, diagnostic tests, and diagnostic accuracy. We searched MEDLINE (OVID interface, 1946 onwards), EMBASE (OVID interface, 1947 onwards), the Cochrane Central Register of Controlled Trials (Wiley interface, current issue), Science Citation Index Expanded (Web of Science interface, 1970 onwards), Conference Proceedings Citation Index-Science (Web of Science interface, 1990 onwards), SCOPUS (1960 onwards), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) (BIREME interface) and WHO Global Index Medicus. The search was supplemented by searching for ongoing studies in WHO’s International Clinical Trials Registry. The literature search was limited to English language and human subjects that available until April 30th, 2015. In addition to searching databases, we contacted individual researchers and authors of major trials to address whether any relevant manuscripts are in preparation or in press. The references of published articles found in the above databases were searched for additional pertinent materials.
Study selection proceeded in three stages: 1) titles/abstracts were screened by a single reviewer according to standard inclusion and exclusion criteria; 2) full manuscripts were obtained and evaluated by two independent reviewers to include or not; 3) two independent reviewers extracted all data. Differences were resolved by a third independent reviewer.
Selection criteria
The inclusion criteria included the following: primary purpose was HCV Ab test evaluation, reported sensitivity and specificity of HCV Ab test kits, and studies published before May 2015. We included observational and randomised control trial (RCT) studies that provided original data from patient specimens. Studies that only reported sensitivity or specificity, conference abstracts, comments or review papers, panel studies, or those that only used reference assays for positive samples were excluded. In this manuscript, a hepatitis panel refers to a laboratory series test in which use the blood with confirmed hepatitis C serostatus to assess the accuracy of a testing kit.
Data extraction
Information on the following variables were extracted from each individual study: first author, total sample size, country (and city) of sampling, sample type (oral fluid, finger prick, venous blood), point-of-care (POC, defined as being able to give a result within 60 min and having the results to guide clinical management in the same encounter), eligibility criteria, reference standard, manufacturer, raw cell numbers (true positives, false negatives, false positives, true negatives), antibody-antigen combo (yes or no), sources of funding, reported conflict of interest, and study population (general population, high risk population and hospitalized population). The high risk population groups include men who have sex with men, sex workers and their clients, transgender people, people who inject drugs and prisoners and other incarcerated people [16]. The hospitalized population was defined as those admitted to a hospital for medical care or observation. We also verified whether assays evaluated in the studies were currently on the market (as of June 1st, 2017), and if this was the case, we also reported the available version of the testing kit (Table 1).
Assessment of methodological quality
Study quality was evaluated using the QUADAS-2 tool [17] and the STARD checklist [18]. QUADAS includes domains to evaluate bias in the following categories: risk of bias (patient selection, index test, reference standard, flow, and timing); applicability concerns (patient selection, index test, reference standard). The STARD checklist consists of a checklist of 25 items and flow diagram that authors can use to ensure that all relevant information is present.
Data analysis and synthesis
Data synthesis
Data were extracted to construct 2 × 2 tables. By comparing with reference standard results, the index test results were categorized as a true positive, a false positive, a false negative, or a true negative. Indeterminate test results were not included in pooled analyses.
Statistical analysis
To estimate test accuracy, we calculated sensitivity and specificity for each study and pooled statistics, along with 95% confidence intervals [19]. We pooled test estimates using the DerSimonian-Laird method, a bivariate random effect model. We did further subanalyses based on reference standard (EIA alone; NAT or immunoblot; EIA, NAT, or immunoblot), brand, sample type, and combination test. We performed all statistical analysis (including heterogeneity, through Q test) using the software R and RevMan 5.3.
Results
Study selection
A total of 11,163 citations were identified, and 6163 duplicates were removed. Each of the 5000 unique citations was examined. A total of 52 research studies were included in the final analysis (Fig. 1) [8, 16, 19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68]. Of the 52 studies, 32 studies evaluated the accuracy of 30 different rapid diagnostic tests (RDTs) [19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50], of which 5 evaluated RDTs compared to EIA alone [25, 26, 31, 34, 49], 13 compared RDT results to NAT or immunoblot [19,20,21,22, 27, 29, 32, 37, 42, 43, 45, 47, 50], and 14 focused on evaluating RDT by comparing with the results of EIA or immunoblot or NAT [23,24,25,26, 30, 34, 35, 38, 39, 41, 44, 48, 49, 51]. Eleven studies evaluated the diagnostic accuracy of oral fluid RDTs [22, 24, 27, 29, 33, 34, 43,44,45, 47, 52].
There were insufficient data to undertake a subanalysis based on HIV co-infection or other co-infections.
Study characteristics
Of the 52 included studies, nine were published before 2000 [37, 38, 42, 53,54,55,56,57,58], 12 studies reported evaluation using oral fluid samples, and 34 studies evaluated POC tests. Of the 52 studies, 41 different brands of testing kits were evaluated (Table 1).
Assessment of the quality of the studies
All studies used a cross-sectional or case–control design. The risk of bias in patient selection, index test, or reference standard was assessed using QUADAS-2 (Table 2). Among the included studies, 25 had at least one category that was considered high risk [19, 22, 25,26,27,28, 30, 31, 34, 36,37,38,39, 41, 45,46,47,48,49,50, 53, 55, 56, 58,59,60,61,62]. The risk of bias in patient selection usually came from a poor description of patient selection and clinical scenario. Bias in the index test was primarily due to a lack of reported blinding while reading test results. Bias in the reference standard was due to the use of multiple reference standards (EIA, NAT, and/or immunoblot). Bias in the flow and timing was primarily due to a lack of reported details.
Diagnostic accuracy
Overall clinical performance of assays
The 52 included studies contributed 127 data points from 52,273 unique test measurements. Some studies contributed additional data points by comparing the accuracy of two or more tests, reporting data from multiple study sites, or reporting the accuracy of a test in more than one type of specimen. The sample sizes of the included studies ranged from 37 to 17,894. Sensitivities of included studies ranged from 22 to 100%, and specificities ranged from 77 to 100%. The overall pooled sensitivity and specificity for all tests were 97% (95% CI: 97%–98%) and 99% (95% CI: 98%-99%) respectively. Figure 2 shows estimates of sensitivity and specificity from each study.
Manufacturers and accuracy of RDTs among included studies
Overall, 32 studies evaluated the accuracy of 30 different RDTs (Table 3). The most commonly evaluated test kit was the OraQuick ADVANCE® from OraSure Technologies.
Pooled test accuracy for RDT versus EIA alone
Overall, five studies evaluated RDTs compared to the EIA alone, with a total sample of 15,943. Of the five studies, sample sizes ranged from 197 to 2754, sensitivities ranged from 83 to 100%, and specificities ranged from 99 to 100%. The pooled sensitivity and specificity were 98% (95% CI 98%-100%) and 100% (95% CI 100%-100%), respectively, while heterogeneity was observed in the included studies (P < 0.001) (Table 3, Additional file 1).
For the three studies that were conducted within the last 10Â years [25, 49, 51], the total sample size was 12,992, with pooled sensitivity and specificity of 99% (95%CI 99%-100%) and 100% (95%CI 100%-100%), respectively.
RDT accuracy compared to NAT or immunoblot
Overall, 13 studies evaluated RDTs compared to NAT or immunoblot [19,20,21,22, 27, 29, 32, 37, 42, 43, 45, 47, 50], with a total sample of 7083. Among these studies, sample sizes ranged from 36 to 549, sensitivities ranged from 76 to 100%, and specificities ranged from 77% to 100%. The pooled sensitivity and specificity were 93% (95% CI 91%-95%) and 98% (95% CI 98%- 99%), respectively, while heterogeneity was observed in the included studies (P < 0.001) (Table 3, Additional file 2).
RDT test accuracy compared to EIA, NAT or Immunoblot
Overall, 14 studies evaluated RDTs by referencing to EIA with NAT and/or immunoblot [25, 26, 31, 33,34,35, 38, 39, 41, 45, 48, 49], with a total sample of 42,212. Of the 14 studies, sample sizes ranged from 168 to 2754, sensitivities ranged from 29 to 100%, and specificities ranged from 90 to 100%. The pooled sensitivity and specificity were 97% (95% CI 96% -98%) and 100% (95% CI 100%-100%), respectively, while heterogeneity was observed in the included studies (P < 0.001) (Table 3, Additional file 3).
Pooled test accuracy for oral versus blood samples
EIAs using oral fluid samples
Overall, 11 studies compared the accuracy of EIAs using oral fluid samples to a blood sample as a reference, with a total sample size of 12,370 [22, 24, 27, 29, 33, 34, 43,44,45, 47, 52]. Of the 12 studies, sample sizes ranged from 37 to 2176, sensitivities ranged from 72 to 100%, and specificities ranged from 91 to 100%. The pooled sensitivity and specificity were 94% (95% CI 93%-96%) and 100% (95% CI 99%-100%), respectively. Heterogeneity was observed in the included studies (P < 0.001) (Table 3, Additional file 4).
Blood samples
Overall, 47 studies used blood samples for evaluations, with a total sample of 90,008. Sample sizes ranged from 37 to 17,894, sensitivities ranged from 29 to 100%, and specificities ranged from 18 to 100%. The pooled sensitivity and specificity were 98% (95% CI 97%-98%) and 98% (95% CI 98%- 98%), respectively. Heterogeneity was observed in the included studies (P < 0.001) (Table 3, Fig. 3).
Pooled test accuracy for OraQuick versus other brands on oral kits
OraQuick
Overall, eight studies reported sensitivity and specificity of OraQuick (OraSure Technologies, PA, USA), with a total sample of 9024 [22, 24, 27, 33,34,35, 43, 45]. The sample size of these studies ranged from 172 to 2183, sensitivities ranged from 90% to 100%, and specificities ranged from 95% to 100%. The pooled sensitivity and specificity were 98% (95% CI 97%-99%) and 100% (95% CI 90%-100%), respectively. Heterogeneity was observed in the included studies (P < 0.001) (Table 3, Additional file 5).
Overall, six studies reported sensitivity and specificity for other three brands of oral kits [29, 43,44,45, 47, 52], with a total sample of 6652. The sample size of these studies ranged from 37 to 1081, sensitivities ranged from 72 to 100%, and specificities ranged from 91 to 100%. The pooled sensitivity and specificity were 88% (95% CI 84%-92%) and 99% (95% CI 99%- 100%), respectively, while heterogeneity was observed between the included studies (P < 0.001) (Table 3, Additional file 6).
Other findings
Our study further found that the overall sensitivity and specificity of studies conducted among general populations were 95% (95% CI 94%-96%) and 99% (95% CI 98%-99%), among high risk populations were 97% (95% CI 96%-98%) and 94% (95% CI 94%-95%), and among hospital patients were 97% (95% CI 96%-98%) and 100% (95% CI 100%-100%), respectively. The overall sensitivity and specificity of the antibody and antigen combo test were 86% (95% CI 79%-99%) and 99% (95% CI: 98%-100%).
GRADE approach (Grading of Recommendations, Assessment, Development and Evaluation to assessing overall quality of evidence
GRADE for RDT versus EIA
HCV Ab RDTs showed comparable sensitivity and specificity compared to that of EIAs. Among the five studies that evaluated RDTs versus EIA, 15,943 of samples were evaluated, and moderate risk of bias was observed (Table 4), but there was a consistent high level of specificity. Since the unit of the analysis varied among studies (Table 4), indirectness was observed. In addition, the overall strength of the pooled evaluation was moderate, with pooled sensitivity and specificity of 99% (95% CI 98%-100%) and 100% (95% CI 100%-100%), respectively. Under the pre-test probability of 5%, the post-test probability after a positive test result is 97%, and the post-test probability after a negative test result is 100%.
GRADE for oral RDT versus blood reference
The use of oral RDTs HCV Ab had comparable sensitivity and specificity compared to blood reference standards (Additional file 7). For the 12 studies evaluated oral RDT versus blood reference, 14,547 samples were evaluated. A moderate risk of bias was observed. Inconsistency was present for sensitivity, as the sensitivities of the included studies varied. But there was a consistent high level of specificity. Since the unit of the analysis varied with each other among the included studies (Table 4), indirectness was observed for included studies. In addition, the overall strength of the pooled evaluation was moderate, with pooled sensitivity and specificity of 94% (95% CI 93%-96%) and 100% (95% CI 100%-100%), respectively. Assuming a pre-test probability of 5%, the post-test probability after a positive test result was 94%, and the post-test probability after a negative test result was 100%.
Discussion
There is a global need to expand HCV diagnostic testing. In this meta-analysis, we found HCV Ab RDTs, including those using oral fluid, showed a high overall sensitivity and specificity compared to laboratory-based EIAs. This extends the literature by including several new studies that were not included in prior reviews, including a sub-analysis that focused on use of RDTs with oral fluid. In addition, the evidence collected from this review was used to inform recommendations in the 2017 WHO guidelines on testing for hepatitis B and C [15]. The evidence for generally high levels of diagnostic accuracy across most brands from this systematic review and meta-analysis supported a strong recommendation for the use of HCV RDTs in WHO testing guidelines [15].
Our data suggest that RDTs can be used for HCV Ab detection in a wide range of clinical settings. For example, for all the included studies, 17 were conducted among general populations, 20 were among high risk populations, and 17 were among hospitalized patients (two studies included two kinds of populations). High HCV Ab RDTs sensitivity and specificity were observed across multiple different populations (including general population, high risk populations, and hospital patients), which is consistent with previous systematic reviews [13, 14, 63]. The use of an EIA to detect HCV Ab followed by NAT to confirm active infection is standard practice for diagnosis of HCV infection and recommended by the US Centers for Disease Control and Prevention and the WHO [64, 65]. However, despite these recommendations, HCV Ab EIA assays have not been widely used because of the complexity of laboratory-based assays, long turnaround time, high cost and requirements for specialized apparatus and trained technicians [13]. To overcome these barriers, RDTs for HCV Ab screening were developed [66]. They obviate the need for multiple follow-up appointments, shorten wait times, and allow for the simplification and decentralization of testing (Additional file 8). However, it is essential for policymakers, government officials, and health care practitioners engaged in HCV screening, care, and treatment to be aware that the performance of individual RDTs for detection of HCV Ab vary widely. Individual diagnostic accuracy for specific brands should be examined to ensure acceptable performance.
Our data suggest that oral fluid RDTs have high sensitivity and specificity. This is consistent with other literature [67]. Tests that can be used with non-invasive samples allow testing to be decentralized further and can be used in outreach settings [68]. Our data suggest that oral tests have a slightly lower pooled sensitivity (94%, 95%CI: 93%-96%) compared to blood-based tests (98%, 95% CI: 97%-98%) but comparable specificity. Oral HCV Ab RDTs tests may be particularly useful in contexts where venepuncture may be difficult, such as subsets of people who inject drugs which have difficult veins to access.
With the increasing availability of DAAs, countries are seeking testing kits with high sensitivity and specificity, in order to allow them to scale up HCV Ab screening, especially among at-risk populations. The advantages and disadvantages of EIAs and RDTs are well established [15]. Performance, cost, and accessibility need to be considered. Determining which tests to deploy at which level of the health care system and for what settings require policy makers to consider the different attributes of laboratory-based EIA versus blood-based or oral RDTs. Potential trade-offs include slightly lower accuracy for greater uptake and acceptability of testing, provision of test results, and linkage to care. Each country needs to decide on which trade-offs or compromises are acceptable, based not only on disease prevalence and the health care infrastructure but also on technical, socioeconomic, cultural, behavioral considerations. For example, they need to be clear on whether it is acceptable to buy Test X which is 10% less accurate than Test Y but is considerably cheaper so that many more people can be tested. In addition, although oral RDTs are less accurate than blood-based RDTs, it may be that oral RDTs will be more acceptable for outreach testing and accessing at-risk populations and allow the control programs to identify more HCV cases. In a low prevalence setting, even a test with 98% specificity can yield more false positive than true positive results. All these trade-offs can be modeled to give an estimate of the cost-effectiveness and potential impact of different strategies for HCV Ab screening.
Our review also underlines some of the common methodological problems encountered in evaluating diagnostic accuracy. Cross-sectional or case–control designs were used by all 52 included studies, introducing a potential risk of bias. These studies used a broad range of reference standards, which makes the pooled performance data less meaningful. Within the evaluation of diagnostic accuracy, even cross-sectional studies in patients with diagnostic uncertainty and direct comparison of test results with an appropriate reference standard can be considered high quality [69]. The majority of the included studies used convenience sampling. In this review, we excluded panel studies because they are not based on clinical settings and our purpose was to generate data that would be relevant in clinical settings as part of detection of HCV Ab.
Most studies that reported HIV or HBV co-infection only reported the test performance of the kits among all samples, instead of disaggregated diagnostic accuracy. There were insufficient data from two studies to undertake a subanalysis based on HIV co-infection. It may be important for policymakers to know the diagnostic accuracy of HCV Ab tests among individuals with co-infections, particularly HIV co-infection [70], and this requires further research among co-infected individuals.
Our study is subject to several limitations. First, we included studies conducted among the general population, hospital patients, and high risk populations. Diagnostic performance can be influenced by disease prevalence and HCV prevalence is variable among these different populations [71, 72]. Second, we detected substantial heterogeneity that could influence our confidence in the review findings [73], but addressed this problem through a series of sub-group stratified analyses. Third, about 20 brands of RDT kits were used in the included studies, and their performance varies considerably. This limited our ability to summarize the accuracy of different brands, with the exception of comparing OraQuick to other brands. Another concern is publication bias, as studies with poor test performance may be less likely to be published, leading to distorted estimates of accuracy [74]. Fourth, since not all HCV RDTs can be performed from oral fluid/capillary whole blood (some require plasma/serum), and some of them require a cold chain for storage and transport, the direct comparison between EIA and RDTs in this meta-analysis would be less meaningful. Fifth, we should note that not all test kits are still on the market and that versions of the tests included in this meta-analysis may have since changed. Finally, statistical heterogeneity was present. But is common in meta-analyses of diagnostic studies. Additional research is important for understanding why the tests perform more poorly in certain populations or settings.
Conclusion
RDTs, including oral tests, have excellent sensitivity and specificity compared to laboratory-based methods for HCV antibody detection across a wide range of settings. National policymakers should consider the performance, cost and accessibility of RDTs into consideration, when selecting assays for use in their national testing algorithms.
Abbreviations
- CI:
-
Confidence interval
- DAAs:
-
Direct acting antivirals
- EIA:
-
Enzyme immunoassay
- FN:
-
False negative
- FP:
-
False positive
- HCV Ab:
-
HCV antibody
- HCV:
-
Hepatitis C Virus
- NAT:
-
Nucleic acid testing
- NPV:
-
Negative predictive value
- POC:
-
Point-of-care
- PPV:
-
Positive predictive value,
- RCT:
-
Randomised control trial
- RDTs:
-
Rapid diagnostic tests
- SE:
-
Sensitivity
- SP:
-
Specificity
- TN:
-
True negative
- TP:
-
True Positive
- WHO:
-
World Health Organization
References
World Health Organization. New recommendations in the updated WHO guidelines for the screening, care and treatment of persons with chronic hepatitis C infection. Geneva: WHO; 2016. http://www.who.int/hepatitis/publications/hepatitis-c-guidelines-2016/en/.
Lavanchy D. The global burden of hepatitis C. Liver Int. 2009;29(s1):74–81.
World Health Organization. Global Hepatitis Report, 2017. Geneva; 2017. http://www.who.int/hepatitis/publications/global-hepatitis-report2017/en/. Accessed 6 Oct 2017.
Feeney ER, Chung RT. Antiviral treatment of hepatitis C. BMJ (Clinical research ed). 2014;348:g3308.
Pawlotsky JM. New hepatitis C therapies: the toolbox, strategies, and challenges. Gastroenterology. 2014;146(5):1176–92.
Soriano V, Labarga P, Fernández-Montero JV, Benito JM, Poveda E, Rallon N, Sánchez C, Vispo E, Barreiro P. The changing face of hepatitis C in the new era of direct-acting antivirals. Antivir Res. 2013;97(1):36–40.
Papatheodoridis G, Tsochatzis E, Hardke S, Wedemeyer H. Barriers to care and treatment for patients with chronic viral hepatitis in europe: a systematic review. Liver Int. 2014;34(10):1452–63.
Zheng H, Li M, Chi B, Wu XX, Wang J, Liu DW. IL28B rs12980275 variant as a predictor of sustained virologic response to pegylated-interferon and ribavirin in chronic hepatitis C patients: a systematic review and meta-analysis. Clin Res Hepatol Gastroenterol. 2015;35(5):576–83.
Manzano-Robleda Mdel C, Ornelas-Arroyo V, Barrientos-Gutierrez T, Mendez-Sanchez N, Uribe M, Chavez-Tapia NC. Boceprevir and telaprevir for chronic genotype 1 hepatitis C virus infection. A systematic review and meta-analysis. Ann Hepatol. 2015;14(1):46–57.
Ambrosino P, Lupoli R, Tarantino P, Di Minno A, Tarantino L, Di Minno MN. Viral hepatitis and anti-phospholipid antibodies positivity: a systematic review and meta-analysis. Dig Liver Dis. 2015;47(6):478–87.
Khodabandehloo M, Roshani D. Prevalence of hepatitis C virus genotypes in Iranian patients: a systematic review and meta-analysis. Hepat Mon. 2014;14(12):e22915.
Khodabandehloo M, Roshani D, Sayehmiri K. Prevalence and trend of hepatitis C virus infection among blood donors in Iran: a systematic review and meta-analysis. J Res Med Sci. 2013;18(8):674–82.
Khuroo MS, Khuroo NS, Khuroo MS. Diagnostic accuracy of point-of-care tests for hepatitis C virus infection: a systematic review and meta-analysis. PLoS One. 2015;10(3):e0121450.
Shivkumar S, Peeling R, Jafari Y, Joseph L, Pant Pai N. Accuracy of rapid and point-of-care screening tests for hepatitis C: a systematic review and meta-analysis. Ann Intern Med. 2012;157(8):558–66.
World Health Organization. Guidelines on hepatitis B and C testing. Geneva; 2017. Available: http://www.who.int/hepatitis/publications/guidelines-hepatitis-c-b-testing/en/. Accessed 6 Oct 2017.
UNAIDS. Unaids terminology guidelines. Geneva: UNADIS; 2015.
Whiting PF, Rutjes AW, Westwood ME, Mallett S, Deeks JJ, Reitsma JB, Leeflang MM, Sterne JA, Bossuyt PM, Group Q. QUADAS-2: a revised tool for the quality assessment of diagnostic accuracy studies. Ann Intern Med. 2011;155(8):529–36.
Bossuyt PM, Reitsma JB, Bruns DE, Gatsonis CA, Glasziou PP, Irwig LM, Moher D, Rennie D, de Vet HC, Lijmer JG, et al. The STARD statement for reporting studies of diagnostic accuracy: explanation and elaboration. Ann Intern Med. 2003;138(1):W1–12.
da Rosa L, Dantas-Correa EB, Narciso-Schiavon JL, Schiavon LL. Diagnostic performance of two point-of-care tests for anti-HCV detection. Hepat Mon. 2013;13(9):e12274.
Al-Tahish G, El-Barrawy MA, Hashish MH, Heddaya Z. Effectiveness of three types of rapid tests for the detection of hepatitis C virus antibodies among blood donors in Alexandria, Egypt. J Virol Methods. 2013;189(2):370–4.
Buti M, Cotrina M, Chan H, Jardi R, Rodriguez F, Costa X, Esteban R, Guardia J. Rapid method for the detection of anti-HCV antibodies in patients with chronic hepatitis C. Rev Esp Enferm Dig. 2000;92(3):140–6.
Cha YJ, Park Q, Kang ES, Yoo BC, Park KU, Kim JW, Hwang YS, Kim MH. Performance evaluation of the OraQuick hepatitis C virus rapid antibody test. Ann Lab Med. 2013;33(3):184–9.
Daniel HD, Abraham P, Raghuraman S, Vivekanandan P, Subramaniam T, Sridharan G. Evaluation of a rapid assay as an alternative to conventional enzyme immunoassays for detection of hepatitis C virus-specific antibodies. J Clin Microbiol. 2005;43(4):1977–8.
Drobnik A, Judd C, Banach D, Egger J, Konty K, Rude E. Public health implications of rapid hepatitis C screening with an oral swab for community-based organizations serving high-risk populations. Am J Public Health. 2011;101(11):2151.
Hess KL, Fisher DG, Reynolds GL. Sensitivity and specificity of point-of-care rapid combination syphilis-HIV-HCV tests. PLoS One. 2014;9(11):e112190.
Hui AY, Chan FK, Chan PK, Tam JS, Sung JJ. Evaluation of anew rapid whole-blood serological test for hepatitis c virus. Acta Virol. 2002;46(1):47–8.
Ibrahim S, Al Attas SA, Mansour GA, Ouda S, Fallatah H. Accuracy of rapid oral HCV diagnostic test among a Saudi sample. Clin Oral Investig. 2015;19(2):475–80.
Ivantes CA, Silva D, Messias-Reason I. High prevalence of hepatitis C associated with familial history of hepatitis in a small town of south Brazil: efficiency of the rapid test for epidemiological survey. Braz J Infect Dis. 2010;14(5):483–8.
Jewett A, Smith BD, Garfein RS, Cuevas-Mota J, Teshale EH, Weinbaum CM. Field-based performance of three pre-market rapid hepatitis C virus antibody assays in STAHR (study to assess hepatitis C risk) among young adults who inject drugs in San Diego, CA. J Clin Virol. 2012;54(3):213–7.
Kant J, Moller B, Heyne R, Herber A, Bohm S, Maier M, Liebert UG, Mossner J, Berg T, Wiegand J. Evaluation of a rapid on-site anti-HCV test as a screening tool for hepatitis C virus infection. Eur J Gastroenterol Hepatol. 2013;25(4):416–20.
Kaur H, Dhanao J, Oberoi A. Evaluation of rapid kits for detection of HIV, HBsAg and HCV infections. Indian J Med Sci. 2000;54(10):432–4.
Kim MH, Kang SY, Lee WI. Evaluation of a new rapid test kit to detect hepatitis C virus infection. J Virol Methods. 2013;193(2):379–82.
Lee SR, Kardos K, Yearwood G, Kurtz L, Roehler M, Feiss G. Results of a multi-center evaluation of a new rapid test for detection of HCV infection using whole blood, serum, plasma and oral fluid. J Hepatol. 2010;52:S271.
Lee SR, Kardos KW, Schiff E, Berne CA, Mounzer K, Banks AT, Tatum HA, Friel TJ, Demicco MP, Lee WM, et al. Evaluation of a new, rapid test for detecting HCV infection, suitable for use with blood or oral fluid. J Virol Methods. 2011;172(1–2):27–31.
Lee SR, Yearwood GD, Guillon GB, Kurtz LA, Fischl M, Friel T, Berne CA, Kardos KW. Evaluation of a rapid, point-of-care test device for the diagnosis of hepatitis C infection. J Clin Virol. 2010;48(1):15–7.
Maity S, Nandi S, Biswas S, Sadhukhan SK, Saha MK. Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India. Virol J. 2012;9:290.
Montebugnoli L, Borea G, Miniero R, Sprovieri G. A rapid test for the visual detection of anti-hepatitis C virus antibodies in whole blood. Clin Chim Acta. 1999;288(1–2):91–6.
Mvere D, Constantine NT, Katsawde E, Tobaiwa O, Dambire S, Corcoran P. Rapid and simple hepatitis assays: encouraging results from a blood donor population in Zimbabwe. Bull World Health Organ. 1996;74(1):19–24.
Njouom R, Tejiokem MC, Zanga MC, Pouillot R, Ayouba A, Pasquier C, Nerrienet E. A cost-effective algorithm for the diagnosis of hepatitis C virus infection and prediction of HCV viremia in Cameroon. J Virol Methods. 2006;133(2):223–6.
Nyirenda M, Beadsworth MB, Stephany P, Hart CA, Hart IJ, Munthali C, Beeching NJ, Zijlstra EE. Prevalence of infection with hepatitis B and C virus and coinfection with HIV in medical inpatients in Malawi. J Infect. 2008;57(1):72–7.
O'Connell RJ, Gates RG, Bautista CT, Imbach M, Eggleston JC, Beardsley SG, Manak MM, Gonzales R, Rentas FJ, Macdonald VW, et al. Laboratory evaluation of rapid test kits to detect hepatitis C antibody for use in predonation screening in emergency settings. Transfusion. 2013;53(3):505–17.
Poovorawan Y, Theamboonlers A, Chumdermpadetsuk S, Thong CP. Comparative results in detection of HCV antibodies by using a rapid HCV test, ELISA and immunoblot. Southeast Asian J Trop Med Public Health. 1994;25(4):647–9.
Scalioni Lde P, Cruz HM, de Paula VS, Miguel JC, Marques VA, Villela-Nogueira CA, Milagres FA, Cruz MS, Bastos FI, Andrade TM, et al. Performance of rapid hepatitis C virus antibody assays among high- and low-risk populations. J Clin Virol. 2014;60(3):200–5.
Smith BD, Drobeniuc J, Jewett A, Branson BM, Garfein RS, Teshale E, Kamili S, Weinbaum CM. Evaluation of three rapid screening assays for detection of antibodies to hepatitis C virus. J Infect Dis. 2011;204(6):825–31.
Smith BD, Teshale E, Jewett A, Weinbaum CM, Neaigus A, Hagan H, Jenness SM, Melville SK, Burt R, Thiede H, et al. Performance of premarket rapid hepatitis C virus antibody assays in 4 national human immunodeficiency virus behavioral surveillance system sites. Clin Infect Dis. 2011;53(8):780–6.
Tagny CT, Mbanya D, Murphy EL, Lefrere JJ, Laperche S. Screening for hepatitis C virus infection in a high prevalence country by an antigen/antibody combination assay versus a rapid test. J Virol Methods. 2014;199:119–23.
Yaari A, Tovbin D, Zlotnick M, Mostoslavsky M, Shemer-Avni Y, Hanuka N, Burbea Z, Katzir Z, Storch S, Margalith M. Detection of HCV salivary antibodies by a simple and rapid test. J Virol Methods. 2006;133(1):1–5.
Yuen MF, Hui CK, Yuen JC, Young JL, Lai CL. The accuracy of SM-HCV rapid test for the detection of antibody to hepatitis C virus. Am J Gastroenterol. 2001;96(3):838–41.
Gao F, Talbot EA, Loring CH, Power JJ, Dionne-Odom J, Alroy-Preis S, Jackson P, Bean CL. Performance of the OraQuick HCV rapid antibody test for screening exposed patients in a hepatitis C outbreak investigation. J Clin Microbiol. 2014;52(7):2650–2.
Kosack CS, Nick S, Shanks L. Diagnostic accuracy evaluation of the ImmunoFlow HCV rapid immunochromatographic test for the detection of hepatitis C antibodies. J Virol Methods. 2014;204:6–10.
Lee SR, Kardos K, Yearwood G, Kurtz L, Roehler M, Feiss G. Results of a multi-center evaluation of a new rapid test for detection of HCV infection using whole blood, serum, plasma and oral fluid. J Hepatol. 2011;52:S271.
Larrat S, Bourdon C, Baccard M, Garnaud C, Mathieu S, Quesada JL, Signori-Schmuck A, Germi R, Blanc M, Leclercq P, et al. Performance of an antigen-antibody combined assay for hepatitis C virus testing without venipuncture. J Clin Virol. 2012;55(3):220–5.
Nalpas B, Thiers V, Pol S, Driss F, Thepot V, Berthelot P, Brechot C. Hepatitis-C Viremia and anti-Hcv antibodies in alcoholics. J Hepatol. 1992;14(2–3):381–4.
Prayson RA, Proffitt MR, Sharp DE, Carey WD. Application of a 2nd-generation recombinant Immunoblot assay to assess hepatitis-C infection. Lab Med. 1993;24(11):732–8.
Feucht HH, Zollner B, Polywka S, Laufs R. Study on reliability of commercially available hepatitis C virus antibody tests. J Clin Microbiol. 1995;33(3):620–4.
Vrielink H, Zaaijer HL, Reesink HW, van der Poel CL, Cuypers HT, Lelie PN. Sensitivity and specificity of three third-generation anti-hepatitis C virus ELISAs. Vox Sang. 1995;69(1):14–7.
O’Flynn N, Jilg W, McQuillan T, Bauer I, Heyermann M, Schulte-Kellinghaus B, Moller D. New HCV assay on the Abbott AxSYM random access analyzer. Clin Lab. 1997;43(5):403–9.
Caudai C, Padula MG, Bastianoni I, Valensin PE, Shyamala V, Han J, Boggiano CA, Almi P. Antibody testing and RT-PCR results in hepatitis C virus (HCV) infection: HCV-RNA detection in PBMC of plasma viremia-negative HCV-Seropositive persons. Infection. 1998;26(3):151–4.
Bonacini M, Lin HJ, Hollinger FB. Effect of coexisting HIV-1 infection on the diagnosis and evaluation of hepatitis C virus. J Acquir Immune Defic Syndr. 2001;26(4):340–4.
Yang JF, Lin YY, Hsieh MH, Tsai CH, Liu SF, Yu ML, Dai CY, Huang JF, Lin WY, Lin ZY, et al. Performance characteristics of a combined hepatitis C virus core antigen and anti-hepatitis C virus antibody test in different patient groups. Kaohsiung J Med Sci. 2011;27(7):258–63.
Yang R, Guan W, Wang Q, Liu Y, Wei L. Performance evaluation and comparison of the newly developed Elecsys anti-HCV II assay with other widely used assays. Clin Chim Acta. 2013;426:95–101.
Yoo SJ, Wang LL, Ning HC, Tao CM, Hirankarn N, Kuakarn S, Yang R, Han TH, Chan RC, Hussain BM, et al. Evaluation of the Elecsys anti-HCV II assay for routine hepatitis C virus screening of different Asian Pacific populations and detection of early infection. J Clin Virol. 2015;64:20–7.
Aspinall EJ, Doyle JS, Corson S, Hellard ME, Hunt D, Goldberg D, Nguyen T, Falck-Ytter Y, Morgan RL, Smith B, Stoove M, Wiktor SZ, Hutchinson S. Targeted hepatitis C antibody testing interventions: a systematic review and meta-analysis. Eur J Epidemiol. 2015;30(2):115–29.
Alter MJ, Kuhnert WL, Finelli L Centers for Disease Control and Prevention. Guidelines for laboratory testing and result reporting of antibody to hepatitis C virus. Centers for Disease Control and Prevention. MMWR Recomm Rep. 2003;52:1-13, 15.
World Health Organization. Guidelines for the screening, care, and treatment of persons with hepatitis C infection. Geneva; 2014. Available: http://www.who.int/hiv/pub/hepatitis/hepatitis-c-guidelines/en/. Accessed 6 Oct 2017.
St John A, Price CP. Economic evidence and point-of-care testing. Clin Biochem Rev. 2013;34(2):61.
D’Angelo RG, Klepser M, Woodfield R, Patel H. Hepatitis C virus screening a review of the OraQuick hepatitis C virus rapid antibody test. J Pharm Technol. 2015;31(1):13–9.
Parisi MR, Soldini L, Vidoni G, Mabellini C, Belloni T, Brignolo L, Negri S, Schlusnus K, Dorigatti F, Lazzarin A. Point-of-care testing for HCV infection: recent advances and implications for alternative screening. New Microbiol. 2014;37(4):449–547.
Schünemann HJ, Oxman AD, Brozek J, Glasziou P, Jaeschke R, Vist GE, Williams JW Jr, Kunz R, Craig J, Montori VM. Grading quality of evidence and strength of recommendations for diagnostic tests and strategies. BMJ (Clinical research ed). 2008;336(7653):1106–10.
Operskalski EA, Kovacs A. HIV/HCV Co-infection: Pathogenesis, Clinical Complications, Treatment, and New Therapeutic Technologies. Curr HIV/AIDS Rep. 2011;8:12.
Shepard CW, Finelli L, Alter MJ. Global epidemiology of hepatitis C virus infection. Lancet Infect Dis. 2005;5(9):558–67.
Leeflang MM, Moons KG, Reitsma JB, Zwinderman AH. Bias in sensitivity and specificity caused by data-driven selection of optimal cutoff values: mechanisms, magnitude, and solutions. Clin Chem. 2008;54(4):729–37.
Irwig L, Macaskill P, Glasziou P, Fahey M. Meta-analytic methods for diagnostic test accuracy. J Clin Epidemiol. 1995;48(1):119–30.
Rothstein HR, Sutton AJ, Borenstein M. Publication bias in meta-analysis: Prevention, assessment and adjustments. John Wiley & Sons; 2006.
Acknowledgements
The authors acknowledge the support of WHO, London School of Hygiene & Tropical Medicine, SESH Global and UNC Project China.
Funding
This work was supported by the National Institutes of Health National Institute of Allergy and Infectious Diseases 1R01AI114310-01 to JT, UNC-South China STD Research Training Centre Fogarty International Centre 1D43TW009532-01 to JT, and the World Health Organization. Publication of this article was funded by the World Health Organization.
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The dataset supporting the conclusions of this article is included within the article’s additional file.
About this supplement
This article has been published as part of BMC Infectious Diseases Volume 17 Supplement 1, 2017: Testing for chronic hepatitis B and C – a global perspective. The full contents of the supplement are available online at https://bmcinfectdis.biomedcentral.com/articles/supplements/volume-17-supplement-1.
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All authors have read and approved the final manuscript. This review was prepared by WT, WC, RP and JDT. AA, DB, OV and JF helped on build searching algorism and performed the literature search. RP and JDT designed the study, and RP, JDT, AA, DB, OV and PE reviewed the manuscript.
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Additional files
Additional file 1:
Pooled test accuracy of HCV Ab RDTs compared to an EIA reference (5 studies). (DOCX 360Â kb)
Additional file 2:
Pooled test accuracy of HCV Ab RDTs compared to a NAT or immunoblot reference (n = 13 studies). (DOCX 621 kb)
Additional file 3:
Pooled test accuracy of HCV Ab RDTs compared to EIA, NAT or immunoblot reference standards (n = 14 studies). (DOCX 1170 kb)
Additional file 4:
Pooled test accuracy for oral HCV Ab RDTs compared to blood as a reference (n = 11 studies). (DOCX 349 kb)
Additional file 5:
Pooled test accuracy for HCV Ab OraQuick kits (n = 8 studies). (DOCX 414 kb)
Additional file 6:
Pooled test accuracy for other brands of oral HCV Ab test kits (n = 6 studies). (DOCX 406 kb)
Additional file 7:
Grade Table. (DOCX 19Â kb)
Additional file 8:
Advantages and Disadvantages of Laboratory based EIAs vs RDTs. (DOCX 14Â kb)
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Tang, W., Chen, W., Amini, A. et al. Diagnostic accuracy of tests to detect Hepatitis C antibody: a meta-analysis and review of the literature. BMC Infect Dis 17 (Suppl 1), 695 (2017). https://doi.org/10.1186/s12879-017-2773-2
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DOI: https://doi.org/10.1186/s12879-017-2773-2
Keywords
- Diagnostic accuracy
- Diagnostic tests
- Hepatitis C
- HCV antibody
- Rapid diagnostic tests