- Research article
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A bio-behavioral intervention to decrease intravaginal practices and bacterial vaginosis among HIV infected Zambian women, a randomized pilot study
BMC Infectious Diseases volume 17, Article number: 338 (2017)
Intravaginal practices (IVP) (cleansing or introducing products inside the vagina for hygiene, health or to please sexual partners) are common among women with HIV. IVP increase the risk of developing bacterial Vaginosis (BV), the most common genital infection associated with transmission of sexually transmitted infections and HIV. This study tested a pilot intervention to reduce IVP and BV in HIV infected women in Zambia.
One hundred twenty-eight HIV infected women engaging in IVP were randomized to two conditions: enhanced standard of care (n = 70) and experimental (n = 58) from May 1, 2013 to February 28, 2014. All participants received a brief educational counseling session on discontinuation of IVP, and those with BV, were provided with medical treatment for BV. Women in the experimental condition received an additional group-based, culturally tailored intervention. Participants completed questionnaires assessing sexual risk factors and IVP and were assessed for BV using Nugent criteria at baseline, 6 months and 12 months.
At 12-month, the proportion of self-reported use of IVPs decreased in the experimental condition: soap (28% vs. 47%); cloth or a rag (19% vs. 38%); and traditional medicines (22% vs. 42%) (all p < 0.05)) compared with the enhanced standard of care condition. The prevalence of BV at 6 and 12 months did not differ by study condition but averaging over study condition, prevalence of BV decreased from 64.2% at baseline to 15.6% at 6 months (p < 0.01) and to 23.6% at 12 months (p = 0.15). Using an enhanced standard of care approach and an enhanced standard of care + a group intervention, IVP and BV decreased over time, but the experimental condition had greater reduction in self-reported use of IVP.
Future studies should address interventions in communities with high burden of IVP, BV and HIV. Interventions that could be administered during routine medical care and decrease IVP and BV are needed, and should be considered part of women’s health programs.
Trial registration number
NCT03134924 (retrospectively registered 21st April 2017).
Intravaginal practice (IVP) include intravaginal cleansing (the cleaning or washing inside the vagina with fingers or other substances for the purpose of removing fluids), or intravaginal insertion (placing something inside the vagina such as powders, creams, herbs, cloth, tissue, regardless of the time they are left inside) [1, 2]. Women engage in IVP for hygiene, health or to please sexual partners. [1, 2] IVP carry deleterious consequences for women’s health as they are associated with the development of bacterial vaginosis and the acquisition of HIV infection. [3,4,5,6,7].
Bacterial vaginosis (BV) is the most common female genital infection and places women at risk for adverse obstetric and gynecologic outcomes (i.e., miscarriages, preterm delivery, and complications associated with gynecological surgeries). [8, 9] In addition, BV increases the risk of HIV acquisition and transmission to sexual partners and newborns. [7, 10, 11] In HIV infected women, IVP is likely responsible for the development of BV and its associated negative health consequences, and may also contribute to increases in lower genital tract inflammation, genital HIV shedding, and subsequent HIV transmission.
Zambia is a sub-Saharan country with high rates of both HIV and BV, and Zambian women living in Lusaka commonly engage in IVP. [12,13,14,15,16] In Zambia, IVP are culturally driven, and typically used to promote vaginal hygiene and to please male partners. [12, 13] Previous studies in sub-Saharan Africa have sought to decrease IVP in women at risk for HIV infection, but the effect of these interventions on rates of BV has been inconsistent. [17,18,19] In addition, previous IVP interventions have not targeted IVP as a method to decrease BV in women with HIV infection, which could have an important impact on the health of HIV infected women and on HIV transmission.
This team previously demonstrated the association of frequent IVP with BV, and evaluated the short-term effect of a bio-behavioral intervention aimed to decrease IVP and BV among HIV-infected Zambian women. [20, 21] However, many HIV risk reduction interventions have achieved behavioral change that are not sustained over time. [22, 23] This study is a pilot study designed to examine the longitudinal impact over 12 months of a culturally tailored bio-behavioral intervention to reduce IVP and BV among HIV-infected women in Zambia. This bio-behavioral intervention includes a biomedical component (prompt diagnosis and treatment of BV) and a behavioral component (behavioral intervention for IVP). In addition, we evaluate the effectiveness of our intervention in reducing lower genital inflammation and HIV shedding. It was hypothesized that participants receiving the bio-behavioral intervention would decrease the use of IVP and BV more than those receiving an enhanced standard of care condition alone. If successful, this bio-behavioral intervention could be considered for HIV infected women as a strategy to decrease BV and its associated detrimental health outcomes.
Institutional review board (University of Miami Miller School of Medicine) and research ethics committee (University of Zambia) approvals were obtained prior to recruitment and any assessment or study related procedures. Interested study candidates were scheduled for an appointment. In a private room, the study was reviewed with study candidates in detail, and all questions were addressed prior to provision of informed consent. Informed consent was obtained from all participants prior to participating in the study.
The study was conducted at a community health centre in urban Lusaka, Zambia. Procedures to develop questionnaires (focus group discussion and key informant interviews) have been previously reported .
Participants were women recruited from May 2013 to February 2014, living with HIV-1 infection, at least 18 years of age, receiving antiretroviral therapy (ART), engaging in intravaginal practices and vaginal intercourse with men in the month prior to enrolment, and living in the Lusaka metropolitan area. Women were excluded from the study if they were pregnant, were on hormonal contraception or had an intrauterine device (IUD) in place to avoid the potential for induced changes in inflammatory cytokines in the genital mucosa due to contraception. Participants were enrolled 7–14 days after the first day of their menses (proliferative phase of the menstrual cycle).
Participants completed a baseline assessment and were then randomly assigned to one of 2 conditions: enhanced standard of care (SOC+) or SOC+ plus group intervention (experimental). A simple random allocation computer generated sequence was utilized. The study statistician did not participate in randomization and the recruiter and laboratory personnel were blinded to the condition assignment. At each visit (baseline, 6 months and 12 months) participants completed audio computer administered self-interviews (ACASI) assessing demographic, sexual risk factors, types and reasons for engaging in IVP, followed by a vaginal examination with collection of genital tract and blood samples. Assessments using ACASI were administered by the study coordinator prior to collection of genital samples. Our pilot data suggested a 22% reduction in BV in women participating in the intervention. A sample size of 64 women per group was calculated for this pilot study to provide 80% power at the two-tailed 0.05 alpha level to detect a significant moderate effect size of 0.5 in differences in BV between the groups.
Enhanced standard of care condition
This study provided an “enhanced standard of care” (SOC+) comparison condition, consisting of a genital tract examination, collection of a vaginal swab with gram stain of vaginal secretions, diagnosis of BV using the Nugent criteria and provision of medication (oral metronidazole) within 48 h of the examination in women with Nugent score of 7–10, regardless of the presence of symptoms. Currently, the existing standard of care for treatment of a vaginal infection such as BV in Zambia consists of syndromic management; i.e., infections are treated with oral metronidazole when women present with malodorous vaginal discharge indicative of BV, without laboratory confirmatory diagnosis. In addition, at baseline, participants received an individual education session on the risk of engaging in IVP, advice to discontinue IVP, and tips for healthy vaginal hygiene, emphasizing avoiding IVP and suggesting replacing IVP by external vaginal cleansing.
Experimental condition: SOC+ plus group intervention
The WASH (Women’s and Sexual Health) intervention included the elements of the enhanced standard of care condition and in addition, a group-based, culturally-tailored intervention to enhance the uptake of the recommendations. The group-based intervention (~10 per group) was a 45-min group session on vaginal health and healthy vaginal practices led by two trained facilitators. Facilitators utilized an intervention manual addressing risks associated with IVP, symptoms of vaginal infections, vaginal health, women’s experience with alternative methods for vaginal care, and communication with partners about vaginal health and the risks associated with IVP. The theoretical basis for the group intervention was the Information Motivation Behavioral Skills (IMB) Model, such that the underlying components of IVP (culture, partner preference, hygiene, health and sexuality factors, motivation, and behavioral skills) were addressed to provide information and motivation to promote vaginal health. The IMB model has been applied to a variety of HIV-related issues as well as in pilot interventions to decrease IVP. [19, 20, 24, 25].
Demographics, sexual risk factors and medical history
Information on demographics, sexual risk factors and medical history was collected. Questions included age, education, employment status, income, marital status, number of children, history of STIs, HIV status of sexual partner, history of exchanging sex for money, number of partners, condom use, time to HIV diagnosis, self-reported CD4 cells per millilitre.
Information on intravaginal practices was collected using a culturally tailored questionnaire assessing specific products used for IVP. Questions to assess product use in the prior month utilized a dichotomous response option: product use (1 = yes, 0 = no). Products used for IVP included water alone, soap, a cloth or sponge or rag, traditional medicines given by traditional healers, herbs or flowers taken directly from the land, lemon, salt and vinegar (see Additional file 1).
Genital tract examination and collection of genital samples
Vaginal samples were collected by a qualified female health care professional. A vaginal speculum was inserted and a vaginal fluid sample was collected using a cotton swab and placed on a glass slide. Gram stained for Nugent scoring was performed within 24 h of collection. Two laboratory technicians were trained prior to study onset. Training included a computer based didactic session at the laboratory site provided by the principal investigator. The reading of all the slides was performed by one trained laboratory technician blinded to the participant condition assignment. In case of unclear reading, the slide was reviewed by a second technician and the chief of the laboratory until an agreement was achieved. Quality control was undertaken by reviewing 20% of the slides at the clinic laboratory by the principal investigator and revealed 100% agreement with scoring of BV.
Cervicovaginal lavage (CVL) was collected after installing 10 ml of sterile saline into the vaginal and cervical areas avoiding the os, and letting it rest for 60 s. CVL samples were transported on ice to the laboratory for analysis within 2 h of collection .
BV was diagnosed using Nugent criteria (normal vaginal flora = Nugent 0–3; intermediate vaginal flora = Nugent 4–6; BV = Nugent 7–10). Diagnosis of BV was done when Nugent score was 7 or above. Abnormal vaginal flora was defined as Nugent score of 4 or above.
Plasma viremia and HIV-1 genital shedding
Plasma viremia and HIV-1 genital shedding were assessed by measuring plasma and CVL HIV-1 ribonucleic acid (RNA) viral load respectively using Abbott m2000® RealTime Polymerase Chain Reaction (PCR) system. CVL was aliquoted into Eppendorf tubes and directly used for determining viral load. Testing was conducted at Centre for Infectious Diseases Research in Zambia (CIDRZ) laboratories. Lower limit of detection was 20 copies per millilitre.
Upon reception of the CVLs at the laboratory, CVL was aliquoted into Eppendorf tubes and spun for 10 min at 1000 x g at 4C. Supernatant was then aliquoted for cytokine analysis. For each study subject, cytokine analysis was performed to quantify the levels of interleukin (IL) IL-6 and IL-8. Cytokine measures were performed using enzyme-linked immunosorbent assay (ELISA). Commercially available cytokines kits were used (Quantikine, R&D Systems, Minneapolis MN). Cytokine analysis was conducted at baseline and 6 months only.
Descriptive analyses were conducted to describe sociodemographic characteristics, sexual risk factors, IVP, Nugent scores, BV, detectable plasma VL and CVL HIV-1 RNA. In the case of continuous variables, sociodemographic and medical history comparisons by condition were performed using t tests or its nonparametric alternative, the Mann-Whitney U test. For comparisons of groups using categorical variables, a chi-square or Fisher’s exact test was performed.
A series of generalized estimating equations (GEE) with a binomial distribution and logit links were conducted with time, condition, and the interaction of time and condition as predictors. Given the low prevalence of some of the practices, generalized linear mixed models (generally used to handle missing data) could not be used. To assess for differences from baseline to 6-month in IL-6 and IL-8, as they are count variables, and to avoid having to transform them into a normal distribution, GEE Poisson or Negative Binomial models with log links were performed. For all models, an independent correlation structure was used. Results from GEEs were followed by least square means analyses to test for differences from baseline to 6 months and 12 month time points, and for differences in the interactions of time and condition. A Bonferroni adjustment was used for multiple comparisons. All analyses were performed using SAS 9.4 for Windows 7. A threshold of p < 0.05 was used to determine statistical significance.
One hundred and twenty-eight women were enrolled and completed baseline assessments. Due to the use of simple randomization, 58 participants were enrolled into the experimental condition and 70 into the SOC+ condition. In the experimental condition 47 (81.0%) women completed the 6 month assessment and 54 (93%) completed the 12 month assessment. Four women did not complete follow-up 12 month assessments: 2 (3%) were lost to follow up, 1 (2%) became pregnant and 1 (2%) died during the study. In the SOC+ condition, 53 (76%) women completed the 6 month assessment and 64 completed the 12 month assessment (91%). Six women were did not complete follow-up 12 month assessments in the SOC+ condition: 4 (7%) women were lost to follow up and 2 (2%) became pregnant during the study. Figure 1 illustrates participation during the study.
Participants’ mean age was 37.3 (SD = 7.7) years and about half had completed primary school. Nearly three-fourths of the women were not working, had low income and were in a primary relationship with a male partner. Almost all had at least one child. More than three-fourths of the women reported treatment for a sexually transmitted infection (STI) in the prior year, and approximately two-thirds reporting knowing that their partner was HIV-infected. Over 90% of participants reported that they had disclosed their HIV status to their partner. About 10% of women reported having sex in exchange for some type of compensation in the prior month. Most women reported one partner in the prior 3 months and inconsistent condom use. The majority of participants had HIV infection diagnosed over 1 year ago and about one-third self-reported having CD4 counts greater than 500. Demographic characteristics and sexual risk factors did not differ significantly between those enrolled in the experimental or SOC+ conditions except that women assigned to the experimental condition less frequently reported being in a primary relationship. Detailed description of demographic characteristics and risk factors by condition can be found in Table 1.
Baseline self-reported use of IVP and laboratory parameters in the sample and by condition.
Products used for IVP
Most women reported using water and soap for IVP (92% and 69% respectively), about half reported using a cloth, sponge or rag. About 20% of women used herbs or flowers from outdoors for IVP, and almost 30% used traditional medicines given by traditional healers for IVP. Women less often endorsed the use of lemon, vinegar or salt (Table 2).
At baseline, approximately three-fourths of the women sampled had abnormal vaginal flora (77%), nearly two-thirds (63%) had BV, and mean Nugent score was high (6.29). About one fifth of the women had detectable plasma HIV-1 VL, and only 15% had genital tract HIV-1 shedding. IVP, rates of abnormal vaginal flora, BV, plasma and genital HIV-1 RNA and cytokines did not differ between the two conditions except level of IL-8 which was higher in women enrolled in the SOC+ condition (Table 2).
IVP and laboratory parameters
Self-reported IVP use over time and by condition
Results collapsed over condition showed that there was a reduction in the self-reported engagement of most IVPs, in particular in the use of water, soap, cloth, sponge or a rag from baseline to 6 months and 12 months. Self-reported use of soap, a cloth, sponge or rag increased from 6 months to 12 months. A reduction was seen in the self-reported use of herbs or flowers and traditional medicines from outdoors from baseline to 6 months, but this reduction was not sustained at 12 months. The changes in IVP over time among women enrolled in both conditions are illustrated in Table 3.
Women in the experimental condition had non statistically significant reduction in the self-reported engagement in certain IVPs. When individual products were evaluated, women in the experimental condition had a greater reduction in the use of soap, cloth, sponge or rag and traditional medicines at 12 months, as illustrated in Table 4.
Laboratory parameters over time and by condition
Results collapsed over condition showed that there was a decrease in the percentages of women with abnormal vaginal flora and with BV from baseline to 6 months and 12 months. No changes in plasma, CVL HIV-1 viral load, IL-6, and IL-8 were noted (see Table 3).
No changes were observed in rates of abnormal vaginal flora, BV, plasma, CVL HIV-1, viral load, IL-6, and IL-8 by condition (see Table 4).
For IL-6 and IL-8, we identified observations with extreme outliers. For IL-6, there was one outlier at 6-months. For IL-8, there were five: one at time baseline, and four at 6 month. However, because the results were identical with or without outliers, the results presented included the outliers to preserve the integrity and accuracy of the data and reported values.
This study compared two strategies to decrease intravaginal practices and bacterial vaginosis in HIV-infected women receiving antiretroviral therapy in urban Lusaka, Zambia. Both strategies provided diagnosis and treatment of BV, and a behavioral intervention to decrease IVP. The experimental condition differed from the enhanced standard of care in having an additional group intervention session. Results support the use of both strategies to enhance the uptake of recommendations to decrease IVP, and to reduce BV. Reductions in the self-reported use of soap, cloths, sponges and traditional medicines were greatest and maintained by women participating in the experimental condition. As seen in previous studies, the impact of the interventions decreased modestly over time, and some practices reverted back to baseline levels, but in general, benefits were maintained at 12 months post baseline.
Other interventions in sub-Saharan Africa have been effective in decreasing IVP, but this decreased was not accompanied by a decrease in BV [18, 19, 27, 28]. These studies have primarily addressed HIV-uninfected women who received intensive behavioral interventions with weekly visits to the study sites, and did not include treatment for BV. [17,18,19, 27]. Although screening asymptomatic women for BV has not been proven to be beneficial or feasible, we propose that prompt diagnosis and treatment for BV in women engaging in IVP appears to have an important effect on improving the vaginal flora, and may be necessary for the behavioral intervention to succeed in maintaining both healthy vaginal hygiene and flora. As we did not see a significant difference in BV in the experimental condition which included a group intervention when compared with enhanced standard of care (individual intervention), we proposed that an individual intervention easily administered by a health care provider can be incorporated into routine medical care of women with HIV who engage in IVP. Although most practices were decreased, the use of traditional medicines, lemon and vinegar was increased at follow up in the SOC+ condition, suggesting that some practices were replaced by others, and evaluation of interventions proposing alternative practices should be considered.
Our study is novel as it is focused on women with HIV infection, in whom the additional potential health consequences of IVP and BV include the risk of HIV transmission to sexual partners and newborns. In addition, study procedures included prompt diagnosis and treatment for BV, and assessment of genital HIV shedding and vaginal inflammation. Although no decrease in HIV genital shedding was observed after the intervention, it should be noted that all participants were receiving antiretrovirals, and it has previously been recognized that transmission in the setting of suppressed HIV viremia is unlikely to occur. [21, 29] However, viral loads are not regularly assessed in Zambia, and HIV-infected women may be unaware of their relative infectivity.
Additional research is merited to evaluate the effect of BV on the vaginal mucosa and the mechanism by which BV increases HIV transmission. It remains unclear whether asymptomatic screening or targeted intervention among HIV-infected women reporting use of IPV may be beneficial.
Some limitations must be considered in interpreting results from this study. Firstly, as a pilot study, the sample size was small, limiting the ability to detect differences in biological outcomes. We chose not to perform blocked randomization, which resulted in an allocation not close to 1:2 and may have resulted in some comparisons to be underpowered and we have closely adhered to the CONSORT guidelines for reporting . Conducting the study in one city and in a single community health center study site limited the generalizability of the results. Women who were on hormonal contraception were excluded from this study, which further limited the generalizability of this study’s findings. Additionally, women in the experimental condition receiving HIV care at the facility may have discussed the group intervention with women enrolled in the SOC + condition, resulting in intrapractice contamination bias. Moreover, the lack of a true control arm hindered the assessment of a true intervention effect. Given the sexual nature of assessing IVP, it is possible that participants may have underreported IVP due to social-desirability. However, self-administered interviews (ACASI) were used to reduce the potential of reporting behaviors that may be perceived as socially desirable behavior. Lastly, as with other experimental designs, the results of this study may be affected due to pre and post comparisons and the Hawthorne or trial effects. Regarding biological outcomes, only BV and not other STIs were assessed, and are important co-factors for BV acquisition and may contribute to HIV genital shedding. The far apart spacing between visits may have missed episodes of BV and times where ART may have not been used.
In summary, we evaluated and compared strategies to decrease both IVP and BV in women with HIV infection. Notwithstanding the limitations of the findings, both strategies appeared effective, with possibly greater benefit obtained from the addition of a group intervention compared to a traditional one-on-one health information intervention.
Interventions that could be easily administered during routine medical care and decrease IVP and BV are needed, and should be considered part of women’s health programs.
Audio computer administered self-interviews
Centre for Infectious Diseases Research in Zambia
Generalized estimating equations
Information motivation behavioral skills
Polymerase chain reaction
Enhanced standard of care.
Sexually transmitted infection
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Thank all those in our research team at the University Teaching Hospital in Lusaka, community sites providing referrals, and the women participating in this study.
The opinions reflected in this report are those of the authors and do not necessarily reflect those of the funding agencies and participating institutions.
This study was funded by grant from the National Institute of Health [K23HD074489]. The Miami Center for AIDS Research (CFAR) at the University of Miami Miller School of Medicine [P30AI073961] provided mentorship and statistical support.
Availability of data and materials
The datasets during and/or analyzed during the current study available from the corresponding author on reasonable request.
All authors have seen and approved the content of this manuscript, have contributed significantly to the work, and declare no conflict of interest. No writing assistance was sought in preparing this manuscript. MA, MC, DJ, MF contributed to the conception and design of the work. MA, EM, VR, KA contributed to the analysis and interpretation of the data. MA, VR, DJ drafted the work and all authors revised it critically for important intellectual content.
The authors declare that they have no competing interests.
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Ethics approval and consent to participate
Institutional review board (University of Miami Miller School of Medicine) and research ethics committee (University of Zambia) approvals were obtained prior to recruitment and any assessment or study related procedures. Informed consent was obtained from all participants prior to participating in the study.
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Alcaide, M.L., Chisembele, M., Malupande, E. et al. A bio-behavioral intervention to decrease intravaginal practices and bacterial vaginosis among HIV infected Zambian women, a randomized pilot study. BMC Infect Dis 17, 338 (2017) doi:10.1186/s12879-017-2436-3
- Intravaginal practices
- bacterial vaginosis
- HIV shedding