- Research article
- Open Access
- Open Peer Review
Characterisation of bla TEM genes and types of β-lactamase plasmids in Neisseria gonorrhoeae – the prevalent and conserved bla TEM-135 has not recently evolved and existed in the Toronto plasmid from the origin
© Muhammad et al.; licensee BioMed Central Ltd. 2014
- Received: 16 June 2014
- Accepted: 18 August 2014
- Published: 22 August 2014
Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a major concern worldwide. It has been recently feared that the bla TEM-1 gene is, via bla TEM-135, evolving into an extended-spectrum β-lactamase (ESBL), which could degrade all cephalosporins including ceftriaxone. The aims of the present study were to characterize the bla TEM genes, types of β-lactamase plasmids, the degradation of ampicillin by TEM-135 compared to TEM-1, and to perform molecular epidemiological typing of β-lactamase-producing N. gonorrhoeae strains internationally.
β-lactamase producing N. gonorrhoeae isolates (n = 139) cultured from 2000 to 2011 in 15 countries were examined using antibiograms, bla TEM gene sequencing, β-lactamase plasmid typing, and N. gonorrhoeae multiantigen sequence typing (NG-MAST). Furthermore, the bla TEM gene was sequenced in the first described Toronto plasmid (pJD7), one of the first Asian plasmids (pJD4) and African plasmids (pJD5) isolated in Canada. The degradation of ampicillin by TEM-135 compared to TEM-1 was examined using a MALDI-TOF MS hydrolysis assay.
Six different bla TEM sequences were identified (among isolates with 125 different NG-MAST STs), i.e. bla TEM-1 (in 104 isolates), bla TEM-135 (in 30 isolates), and four novel bla TEM sequences (in 5 isolates). The bla TEM-1 allele was only found in the African and Asian plasmids, while all Rio/Toronto plasmids possessed the bla TEM-135 allele. Most interesting, the first described gonococcal Toronto plasmid (pJD7), identified in 1984, also possessed the highly conserved bla TEM-135 allele. The degradation of ampicillin by TEM-135 compared to TEM-1 was indistinguishable in the MALDI-TOF MS hydrolysis assay.
bla TEM-135, encoding TEM-135, is predominantly and originally associated with the Rio/Toronto plasmid and prevalent among the β-lactamase producing gonococcal strains circulating globally. bla TEM-135 does not appear, as previously hypothesized, to have recently evolved due to some evolutionary selective pressure, for example, by the extensive use of extended-spectrum cephalosporins worldwide. On the contrary, the present study shows that bla TEM-135 existed in the Toronto plasmid from its discovery and that bla TEM-135 is highly conserved (not further evolved in the past >30 years). Nevertheless, international studies for monitoring the presence of different bla TEM alleles, the possible evolution of the bla TEM-135 allele, and the types of β-lactamase producing plasmids, remain imperative.
- Antimicrobial resistance
- bla TEM-1
- bla TEM-135
- Rio/Toronto plasmid, Extended-spectrum β-lactamase (ESBL)
Gonorrhoea is the most prevalent bacterial sexually transmitted infection (STI) globally, according to the latest estimates by the World Health Organization (WHO) . No vaccine is available and, accordingly, appropriate prevention, and particularly effective diagnosis and antimicrobial treatment are the cornerstones for control of gonorrhoea. Neisseria gonorrhoeae has developed resistance to all antimicrobials previously recommended for first-line empiric monotherapy, such as penicillins, tetracyclines, macrolides and fluoroquinolones [2–5]. Clinical resistance to the extended-spectrum cephalosporins (ESCs) has also been verified in the latest years in many countries [6–19]. Furthermore, the first three extensively-drug resistant (XDR ) gonococcal strains were recently described; all displayed a high-level of resistance to ceftriaxone, the last remaining option for empiric first-line antimicrobial monotherapy in most countries globally [9, 18, 20]. All the reported decreased susceptibility and resistance to ESCs has been due to an accumulation of chromosomal resistance determinants [2, 4, 5].
In 1976 the first β-lactamase producing N. gonorrhoeae strains, resulting in high-level resistance to penicillins but not affecting the MICs of ESCs, were reported [21, 22]. These strains produced the traditional TEM-1 β-lactamase that hydrolyses the cyclic amide bond in the β-lactam ring . β-lactamase producing gonococcal strains are currently widespread internationally [2, 4]. The bla TEM genes are located on a family of related β-lactamase plasmids, of which the most frequently described have been the Asian, African, and the mainly indistinguishable Rio and Toronto plasmids (named based on their epidemiological origin) [4, 24–26]. However, other types of β-lactamase producing plasmids have also been described in gonococci, e.g. Nimes, New Zealand, Australian and Johannesburg [4, 26–28]. The Asian plasmid has been considered to be the ancestral plasmid from which the other plasmids evolved through deletions and/or insertions. Accordingly, these β-lactamase producing plasmids may be characterised as either deletion derivates of the Asian plasmid (Africa, Rio/Toronto and Johannesburg) or insertion derivatives of either the Asian (New Zealand) or African (Nimes) plasmids [4, 24–27].
Worryingly, the bla TEM-1 gene, encoding the TEM-1 β-lactamase, needs only a few specific single nucleotide polymorphisms (SNPs) to evolve into a gene encoding an extended-spectrum β-lactamase (ESBL), which could degrade all ESCs including ceftriaxone [29–32]. The spread of a potent ESBL, which also degrades ceftriaxone, in the gonococcal population might rapidly result in untreatable gonorrhoea in most settings worldwide. It has been hypothesized that the gonococcal bla TEM-1 has recently evolved into bla TEM-135, which originally was identified in Salmonella enterica subsp. enterica serovar Typhimurium  and might be a precursor in the evolution into an ESBL gene [29, 34, 35]. TEM-135 producing gonococcal isolates have been described in 2004 and 2008 in Japan , from 2005 to 2007 in Thailand [34, 36], and in 2007 and 2012 in China . TEM-135, which only differs from TEM-1 by one SNP (T→C at position 539) resulting in the amino acid alteration M182T, requires solely one additional specific SNP to evolve into an ESBL such as TEM-20 [29–32, 34]. In those ESBLs, the M182T alteration presumably stabilizes the active site topology reorganized by other mutations, which collaboratively results in the emergence of a stable ESBL [34, 35, 37]. However, there have been few reports, and none outside Asia, regarding surveillance of the different types of bla TEM genes and β-lactamase producing plasmids in the gonococcal strains circulating worldwide.
The aims of this study were to characterize the bla TEM genes, the types of β-lactamase plasmids, the degradation of ampicillin by TEM-135 compared to TEM-1, and to perform molecular epidemiological typing of β-lactamase-producing N. gonorrhoeae isolates cultured in 2000–2011 in 15 countries.
β-lactamase producing N. gonorrhoeaeisolates
In total, 139 β-lactamase producing N. gonorrhoeae isolates were examined, including 136 N. gonorrhoeae clinical isolates (collected from 2000 to 2011 in 15 WHO European (n = 40), African (n = 22), American (North and Latin America) (n = 10), Southeast Asian (n = 33) or Western Pacific (n = 31) countries) and three of the 2008 WHO N. gonorrhoeae reference strains, i.e. WHO M (isolated in the Philippines, 1992), WHO N (Australia, 2001), and WHO O (Canada, 1991) . All isolates were cultured on selective culture media for 16–18 h at 37°C, in a 5% CO2-enriched atmosphere. N. gonorrhoeae isolates were identified using characteristic colony morphology, Gram staining, positive oxidase test, a rapid carbohydrate utilization test, and the Phadebact GC Monoclonal Test (Boule Diagnostics AB, Huddinge, Sweden). All examined gonococcal isolates were cultured and stored as part of the routine diagnostics (standard care) and no patient identification information was used. Furthermore, to elucidate when bla TEM-135 emerged DNA from three previously published strains was also investigated. Those represented the first Toronto plasmid described (pJD7 [39, 40], identified in 1984), one of the first Asian plasmids (pJD4 [39–41], identified in the late-1970s) and one of the first African plasmids (pJD5 [39–41], identified in the late-1970s) isolated in Canada.
Antimicrobial susceptibility testing
MICs (mg/L) of the isolates for ampicillin, cefixime, ceftriaxone, azithromycin, ciprofloxacin, and spectinomycin were determined using the Etest method (AB bioMérieux, Solna, Sweden), according to the instructions from the manufacturer. Breakpoints for susceptibility, intermediate susceptibility and resistance in accordance to the European Committee on Antimicrobial Susceptibility Testing (EUCAST; http://www.eucast.org) were used. β-lactamase production was identified using nitrocefin solution (Oxoid, Basingstoke, Hants, England). The 2008 N. gonorrhoeae WHO reference strains  were used for quality control in all antimicrobial susceptibility testing.
β-lactamase hydrolysis assay
To examine the degradation of ampicillin by TEM-135 compared to TEM-1, a selection of six β-lactamase producing gonococcal isolates (three bla TEM-135 and three bla TEM-1 isolates) were investigated using a hydrolysis assay. The non-β-lactamase producing N. gonorrhoeae reference strain WHO F  was included as negative control. All isolates were cultured on New York City agar media plates and incubated for 16–18 h at 37°C in a 5% CO2-enriched atmosphere. The hydrolysis assay was performed as previously described  on a Microflex (Bruker Daltonics, GmbH, Germany), by recording spectra in the mass range of 0–1000 Da after incubation for 15 minutes, 1 h, 2 h and 3 h. For calibration, the Peptide calibration standard II (Bruker Daltonics, GmbH, Germany) was used. The peaks utilized for calibration was CCA [M + H] + at 190.05 Da, CCA [2 M + H] + at 379.09 Da and Bradykinin (1–7) peak [M + H] + at 757.40 Da. Generated spectra were manually examined using the Flex Analysis 3.1 software (Bruker Daltonics, GmbH, Germany), and peaks correlating to hydrolyzed or intact ampicillin  were identified.
Plasmid DNA extraction
The QIAGEN Spin Miniprep Kit (QIAGEN, Hilden, Germany) was used to extract and purify plasmid DNA, according to the instructions from the manufacturer. DNA was stored at 4°C prior to subsequent analysis.
Genomic DNA extraction
Genomic DNA was extracted using the robotized NorDiag Bullet (NorDiag ASA, Oslo, Norway) and the BUGS’n BEADS STI-fast kit (NorDiag ASA, Oslo, Norway), according to the instructions from the manufacturer. DNA was stored at 4°C prior to subsequent analysis.
Multiplex PCR was performed for β-lactamase plasmid typing on all isolates as previously described using the primers BL1, BL2, BL3, and BL4 .
Sequencing of the bla TEMgene
The entire coding region, including the signal peptide of 23 amino acids (GenBank accession number AAR25033), of bla TEM was PCR amplified in a LightCycler real-time PCR System (Roche Molecular Biochemicals, Mannheim, Germany) and subsequently sequenced as previously described . Multiple-sequence alignments of nucleotide and amino acid sequences were performed using the BioEdit (version 5.0.9) software. For comparison and numbering of the amino acid positions, all amino acid sequences identified in the present study were compared to sequences at the Lactamase Engineering Database (http://www.laced.uni-stuttgart.de/) as well as at the β-Lactamase Classification and Amino Acid Sequences for TEM, SHV and OXA Extended-Spectrum and Inhibitor Resistant Enzymes database (http://www.lahey.org/Studies/). The scheme proposed by Ambler et al.  was used for numbering of amino acids.
Molecular epidemiological characterisation using Neisseria gonorrhoeaemultiantigen sequence typing (NG-MAST)
Amplification and sequencing of the more variable segments of the porB and tbpB genes examined in NG-MAST [44, 45] was performed as previously described . For assignment of porB and tbpB allele numbers as well as NG-MAST STs, the NG-MAST website (http://www.ng-mast.net/) was used.
Antimicrobial susceptibility of β-lactamase producing N. gonorrhoeaeisolates (n = 139) from 2000 to 2011
The majority (83.5%, n = 116/139) of isolates were resistant to ampicillin and the remaining 16.5% (n = 23) had an intermediate susceptibility to ampicillin. Furthermore, 80.6% (n = 112) and 6.5% (n = 9) of the isolates were resistant to ciprofloxacin and azithromycin, respectively. Only one isolate (0.7%) was resistant to cefixime (MIC = 0.25 mg/L) and no isolates were resistant to ceftriaxone or spectinomycin.
β-lactamase plasmid types and bla TEMalleles
Type of bla TEM allele, β-lactamase producing plasmid, number of NG-MAST STs, and year and WHO Region of isolation of N. gonorrhoeae isolates cultured in 2000–2011 in 15 countries
β-lactamase encoding gene (No. of isolates)
β-lactamase producing plasmid types (%)
Number different NG-MAST STs
Year (WHO Region) of isolation
bla TEM-1 (104)
African (85.6), Asian (14.4)
2000-2011 (All Regions a )
bla TEM-135 (30)
Rio/Toronto (86.7), Asian (10), African (3.3),
2000-2009 (All Regions a )
bla TEM-P14S b (2)
bla TEM-P14T b (1)
bla TEM-E110K (1)
bla TEM-G228S (1)
2009 (Western Pacific)
bla TEM-135 c
bla TEM-1 c
bla TEM-1 c
Most interesting, the first described Toronto plasmid (pJD7 [39, 40]), isolated in 1984, also possessed the bla TEM-135 allele, while the Asian plasmid (pJD4 [39–41]) and African plasmid (pJD5 [39–41]), isolated in the late-1970s, had the bla TEM-1 allele.
The mean MIC of ampicillin in TEM-135 and TEM-1 producing isolates was 16 mg/L and 12 mg/L, respectively. Furthermore, the mean MIC of ceftriaxone in the TEM-135 and TEM-1 producing isolates was identical, that is, 0.016 mg/L. The isolates containing the novel bla TEM alleles had also low MICs of ceftriaxone (MIC = 0.003-0.006 mg/L).
Degradation of ampicillin by TEM-135 and TEM-1
In the hydrolysis assay, the MALDI-TOF MS spectra showed that the degradation of ampicillin by TEM-135 and TEM-1 was indistinguishable and both β-lactamases degraded ampicillin in 15 minutes. For the non-β-lactamase producing N. gonorrhoeae reference strain WHO F , included as negative control, no hydrolysis was observed even after three hours of incubation (Additional file 1: Figure S1).
Genotyping using N. gonorrhoeaemultiantigen sequence typing (NG-MAST)
In total, 125 different NG-MAST STs were identified, of which 87 had not been previously described. The most frequent STs were ST6058 (3.5% of isolates), ST6057 (2.9%), and ST1288 (2.2%). The majority of STs (93.6% of the STs) were represented by one isolate, whereas five ST were represented by two isolates. Due to the heterogeneity of the isolates and the many STs identified, it was not possible to correlate any β-lactamase plasmid type to any specific NG-MAST ST. Notably, the 30 gonococcal isolates possessing the bla TEM-135 allele were assigned to 29 different NG-MAST STs.
The present study investigated the characteristics of bla TEM genes and their association with β-lactamase plasmid type, the degradation of ampicillin by TEM-135 compared to TEM-1, and the molecular epidemiology of international β-lactamase-producing N. gonorrhoeae isolates. The isolates comprised a large collection of temporally (cultured from 2000 to 2011), geographically (from 15 WHO European, African, American (North and Latin America), Southeast Asian or Western Pacific countries) and genetically diverse (125 NG-MAST STs) gonococcal strains. Clearly, N. gonorrhoeae strains producing TEM-1 are widespread and all these strains carried the bla TEM-1 allele on an African or Asian plasmid. The highly conserved bla TEM-135 allele was predominantly found on the Rio/Toronto plasmids, i.e. only four of the 30 identified bla TEM-135 alleles were carried on African (n = 1) or Asian (n = 3) plasmids. In fact, all the identified Rio/Toronto plasmids in 26 genetically highly diverse isolates (26 different NG-MAST STs) contained a bla TEM-135 allele and according to our best knowledge bla TEM-1 has only been described in one Rio/Toronto plasmid ever . These results strongly indicate that the Rio/Toronto-type plasmid is the origin of the bla TEM-135 allele. Interestingly, in the present study the bla TEM-135 allele was also identified on the first reported Toronto plasmid (pJD7 [39, 40]), found in a gonococcal isolate from 1984. This shows that the bla TEM-135 allele has not recently evolved and, on the contrary, was present in the Toronto plasmid from its discovery. This might also question the evolutionary origin of the Rio/Toronto plasmids, i.e. that these plasmids emerged directly through a deletion of 2273 bp in the Asian plasmid [25, 27], in which the bla TEM-135 allele is rare internationally. Nevertheless, as shown in the present study the bla TEM-135 allele can also be found on African-type and Asian-type plasmids. Thus, it cannot be excluded that those bla TEM-135 alleles might have evolved from bla TEM-1 alleles by a SNP in those plasmids. However, considering how conserved the gonococcal bla TEM alleles appear to be, a more plausible explanation might be that these bla TEM-135 alleles were acquired by horizontal transfer from strains possessing the bla TEM-135 allele on a Rio/Toronto plasmid. This hypothesis is further supported by the lack of antimicrobial selective pressure for the evolution of bla TEM-1 to bla TEM-135. Accordingly, similar MICs of ampicillin and ceftriaxone, and of other antimicrobials, were displayed by the TEM-135 and TEM-1 producing isolates, and the two different TEM enzymes showed an indistinguishable degradation of ampicillin in the MALDI-TOF MS hydrolysis assay. Nevertheless, for detailed measurement of the kinetics of the ampicillin hydrolysis appropriate kinetic experiments (Kcat/Km) with a purified protein would be required. All the novel bla TEM alleles found in the present study were carried by African (four strains) or Asian (one strain) plasmids, and none of these five isolates had any enhanced MIC of ceftriaxone.
bla TEM-135, encoding TEM-135, is predominantly and originally associated with the Rio/Toronto plasmid and prevalent among the β-lactamase producing gonococcal strains circulating globally. bla TEM-135 does not appear, as previously hypothesized, to have recently evolved due to, for example, some evolutionary selective pressure by the extended-spectrum cephalosporins. On the contrary, bla TEM-135 existed in the Toronto plasmid from its discovery. The present study indicates that bla TEM-135 instead is highly conserved (not further evolved in the past >30 years). Accordingly, despite the extensive use of extended-spectrum cephalosporins globally no additional SNP has evolved in bla TEM-135. The reasons for this remain unknown, however, both the lack of evolutionary positive selection for such SNPs and a decreased biological fitness of gonococcal strains with the resulting TEM alleles might be involved. Even so, only one specific SNP added to the bla TEM-135 allele would produce an ESBL (e.g. an additional G238S alteration to evolve into TEM-20), which would be able to degrade all extended-spectrum cephalosporins, possibly rendering gonorrhoea an untreatable infection. Accordingly, international studies for monitoring and enhancing our understanding of, in addition to the chromosomal ESC resistance determinants, the presence of different bla TEM alleles, the possible evolution of the bla TEM-135 allele, and the β-lactamase producing plasmids, remain imperative.
This study was supported by grants from the Research Committee of Örebro County and the Örebro University Hospital Research Foundation, Örebro, Sweden.
- World Health Organization: Global Incidence and Prevalence of Selected Curable Sexually Transmitted Infections - 2008. 2012, Geneva: World Health Organization, Available at: http://www.who.int/reproductivehealth/publications/rtis/2008_STI_estimates.pdf; (Accessed: July 24, 2014)Google Scholar
- Lewis DA: The gonococcus fights back: is this time a knock out?. Sex Transm Infect. 2010, 86: 415-421.View ArticlePubMedGoogle Scholar
- Tapsall JW, Ndowa F, Lewis DA, Unemo M: Meeting the public health challenge of multidrug- and extensively drug-resistant Neisseria gonorrhoeae. Expert Rev Anti Infect Ther. 2009, 7: 821-834.View ArticlePubMedGoogle Scholar
- Unemo M, Shafer WM: Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution and future. Clin Microbiol Rev. 2014, 27: 587-613.View ArticlePubMedPubMed CentralGoogle Scholar
- Unemo M, Nicholas RA: Emergence of multi-drug resistant, extensively drug-resistant and untreatable gonorrhea. Future Microbiol. 2012, 7: 1401-1422.View ArticlePubMedPubMed CentralGoogle Scholar
- Deguchi T, Yasuda M, Yokoi S, Ishida K, Ito M, Ishihara S, Minamidate K, Harada Y, Tei K, Kojima K, Tamaki M, Maeda S: Treatment of uncomplicated gonococcal urethritis by double-dosing of 200 mg cefixime at a 6-h interval. J Infect Chemother. 2003, 9: 35-39.View ArticlePubMedGoogle Scholar
- Yokoi S, Deguchi T, Ozawa T, Yasuda M, Ito S, Kubota Y, Tamaki M, Maeda S: Threat to cefixime treatment for gonorrhea. Emerg Infect Dis. 2007, 13: 1275-1277.PubMedPubMed CentralGoogle Scholar
- Ison CA, Hussey J, Sankar KN, Evans J, Alexander S: Gonorrhoea treatment failures to cefixime and azithromycin in England, 2012. Euro Surveill. 2011, 16 (14): pii=19833-Google Scholar
- Unemo M, Golparian D, Nicholas R, Ohnishi M, Gallay A, Sednaoui P: High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure. Antimicrob Agents Chemother. 2012, 56: 1273-1280.View ArticlePubMedPubMed CentralGoogle Scholar
- Unemo M, Golparian D, Stary A, Eigentler A: First Neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in Austria, 2011. Euro Surveill. 2011, 16 (43): pii=19998-Google Scholar
- Unemo M, Golparian D, Syversen G, Vestrheim DF, Moi H: Two cases of verified clinical failures using internationally recommended first-line cefixime for gonorrhoea treatment, Norway, 2010. Euro Surveill. 2010, 15 (47): pii=19721-Google Scholar
- Lewis DA, Sriruttan C, Müller EE, Golparian D, Gumede L, Fick D, de Wet J, Maseko V, Coetzee J, Unemo M: Phenotypic and genetic characterization of the first two cases of extended-spectrum-cephalosporin-resistant Neisseria gonorrhoeae infection in South Africa and association with cefixime treatment failure. J Antimicrob Chemother. 2013, 68: 1267-1270.View ArticlePubMedGoogle Scholar
- Allen VG, Mitterni L, Seah C, Rebbapragada A, Martin IE, Lee C, Siebert H, Towns L, Melano RG, Low DE: Neisseria gonorrhoeae treatment failure and susceptibility to cefixime in Toronto, Canada. JAMA. 2013, 309: 163-170.View ArticlePubMedGoogle Scholar
- Unemo M, Golparian D, Potočnik M, Jeverica S: Treatment failure of pharyngeal gonorrhoea with internationally recommended first-line ceftriaxone verified in Slovenia, September 2011. Euro Surveill. 2012, 17: 1-4.Google Scholar
- Unemo M, Golparian D, Hestner A: Ceftriaxone treatment failure of pharyngeal gonorrhoea verified by international recommendations, Sweden, July 2010. Euro Surveill. 2011, 16: 1-3.Google Scholar
- Tapsall J, Read P, Carmody C, Bourne C, Ray S, Limnios A, Sloots T, Whiley D: Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods. J Med Microbiol. 2009, 58: 683-687.View ArticlePubMedGoogle Scholar
- Chen YM, Stevens K, Tideman R, Zaia A, Tomita T, Fairley CK, Lahra M, Whiley D, Hogg G: Failure of ceftriaxone 500 mg to eradicate pharyngeal gonorrhoea, Australia. J Antimicrob Chemother. 2013, 68: 1445-1447.View ArticleGoogle Scholar
- Ohnishi M, Golparian D, Shimuta K, Saika T, Hoshina S, Iwasaku K, Nakayama S, Kitawaki J, Unemo M: Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: Detailed characterization of the first strain with high-level resistance to ceftriaxone. Antimicrob Agents Chemother. 2011, 55: 3538-3545.View ArticlePubMedPubMed CentralGoogle Scholar
- Read PJ, Limnios EA, McNulty A, Whiley D, Lahra LM: One confirmed and one suspected case of pharyngeal gonorrhoea treatment failure following 500 mg ceftriaxone in Sydney, Australia. Sex Health. 2013, 10: 460-462.View ArticlePubMedGoogle Scholar
- Camara J, Serra J, Ayats J, Bastida T, Carnicer-Pont D, Andreu A, Ardanuy C: Molecular characterization of two high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain. J Antimicrob Chemother. 2012, 67: 1858-1860.View ArticlePubMedGoogle Scholar
- Ashford WA, Golash RG, Hemming VG: Penicillinase-producing Neisseria gonorrhoeae. Lancet. 1976, 2: 657-658.View ArticlePubMedGoogle Scholar
- Phillips I: Beta-lactamase-producing, penicillin-resistant gonococcus. Lancet. 1976, 2: 656-657.View ArticlePubMedGoogle Scholar
- Bergström S, Norlander L, Norqvist A, Normark S: Contribution of a TEM-1-like beta-lactamase to penicillin resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1978, 13: 618-623.View ArticlePubMedPubMed CentralGoogle Scholar
- Pagotto F, Aman AT, Ng L-K, Yeung KH, Brett M, Dillon JA: Sequence analysis of the family of penicillinase-producing plasmids of Neisseria gonorrhoeae based on DNA sequencing. Plasmid. 2000, 43: 24-34.View ArticlePubMedGoogle Scholar
- Palmer HM, Leeming JP, Turner A: A multiplex polymerase chain reaction to differentiate β-lactamase plasmids of Neisseria gonorrhoeae. J Antimicrob Chemother. 2000, 45: 777-782.View ArticlePubMedGoogle Scholar
- Dillon JR, Yeung KH: β-lactamase plasmids and chromosomally mediated antibiotic resistance in pathogenic Neisseria species. Clin Microbiol Rev. 1989, 2: S125-S133.View ArticlePubMedPubMed CentralGoogle Scholar
- Müller EE, Fayemiwo SA, Lewis DA: Characterization of a novel β-lactamase-producing plasmid in Neisseria gonorrhoeae: sequence analysis and molecular typing of host gonococci. J Antimicrob Chemother. 2011, 66: 1514-1517.View ArticlePubMedGoogle Scholar
- Trembizki E, Buckley C, Lawrence A, Lahra M, Whiley D: On behalf of the GRAND study investigators: Characterisation of a novel Neisseria gonorrhoeae penicillinase-producing plasmid, Australia 2012. Antimicrob Agents Chemother. 2014, 58: 4984-4985.View ArticlePubMedPubMed CentralGoogle Scholar
- Ohnishi M, Ono E, Shimuta K, Watanabe H, Okamura N: Identification of TEM-135 β-lactamase in penicillinase-producing Neisseria gonorrhoeae strains in Japan. Antimicrob Agents Chemother. 2010, 54: 3021-3023.View ArticlePubMedPubMed CentralGoogle Scholar
- Arlet G, Goussard S, Courvalin P, Philippon A: Sequences of the genes for the TEM-20, TEM-21, TEM-22, and TEM-29 extended-spectrum β-lactamases. Antimicrob Agents Chemother. 1999, 43: 969-971.PubMedPubMed CentralGoogle Scholar
- Michael GB, Butaye P, Cloeckaert A, Schwarz S: Genes and mutations conferring antimicrobial resistance in Salmonella: An update. Microb Infect. 2006, 8: 1898-1914.View ArticleGoogle Scholar
- Huang W, Palzkill T: A natural polymorphism in beta-lactamase is a global suppressor. Proc Natl Acad Sci U S A. 1997, 94: 8801-8806.View ArticlePubMedPubMed CentralGoogle Scholar
- Pasquali F, Kehrenberg C, Manfreda G, Schwarz S: Physical linkage of Tn3 and part of Tn1721 in a tetracycline and ampicillin resistance plasmid from Salmonella Typhimurium. J Antimicrob Chemother. 2005, 55: 562-565.View ArticlePubMedGoogle Scholar
- Nakayama S, Tribuddharat C, Prombhul S, Shimuta K, Srifuengfung S, Unemo M, Ohnishi M: Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand. Antimicrob Agents Chemother. 2012, 56: 916-920.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen SC, Yin YP, Dai XQ, Yu RX, Han Y, Sun HH, Ohnishi M, Unemo M, Chen XS: Prevalence and molecular epidemiological typing of penicillinase-producing Neisseria gonorrhoeae and their bla(TEM-135) gene variants in Nanjing, China. Sex Transm Dis. 2013, 40: 872-876.View ArticlePubMedGoogle Scholar
- Srifeungfung S, Roongpisuthipong A, Asavapiriyanont S, Lolekha R, Tribuddharat C, Lokpichart S, Sungthong P, Tongtep P: Prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in HIV-seropositive patients and gonococcal antimicrobial susceptibility: an update in Thailand. J Infect Dis. 2009, 62: 467-470.Google Scholar
- Orencia MC, Yoon JS, Ness JE, Stemmer WP, Stevens RC: Predicting the emergence of antibiotic resistance by directed evolution and structural analysis. Nat Struct Biol. 2001, 8: 238-242.View ArticlePubMedGoogle Scholar
- Unemo M, Fasth O, Fredlund H, Limnios A, Tapsall JW: Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposes. J Antimicrob Chemother. 2009, 63: 1142-1151.View ArticlePubMedGoogle Scholar
- Yeung KH, Dillon JR, Pauzé M, Wallace E: A novel 4.9-kilobase plasmid associated with an outbreak of penicillinase-producing Neisseria gonorrhoeae. J Infect Dis. 1986, 153: 1162-1165.View ArticlePubMedGoogle Scholar
- Yeung KH, Dillon JR: In vitro transcription/translation products and molecular characterization of naturally occurring and in vitro deletion of the 7.2 kb plasmid of Neisseria gonorrhoeae. p. 209 215. The Pathogenic Neisseriae. Proceedings of the 4th international symposium, Asilomar, California, 21 25 October 1984. Edited by: Schoolnik GK. 1985, Washington, D.C: American Society for Microbiology (ASM)Google Scholar
- Pagotto F, Dillon JA: Multiple origins and replication proteins influence biological properties of beta-lactamase-producing plasmids from Neisseria gonorrhoeae. J Bacteriol. 2001, 183: 5472-5481.View ArticlePubMedPubMed CentralGoogle Scholar
- Sparbier K, Schubert S, Weller U, Boogen C, Kostrzewa M: Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based functional assay for rapid detection of resistance against β-lactam antibiotics. J Clin Microbiol. 2012, 50: 927-937.View ArticlePubMedPubMed CentralGoogle Scholar
- Ambler RP, Coulson AF, Frère JM, Ghuysen JM, Joris B, Forsman M, Levesque RC, Tiraby G, Waley SG: A standard numbering scheme for the class A beta-lactamases. Biochem J. 1991, 276: 269-270.View ArticlePubMedPubMed CentralGoogle Scholar
- Martin IM, Ison CA, Aanensen DM, Fenton KA, Spratt BG: Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. J Infect Dis. 2004, 189: 1497-1505.View ArticlePubMedGoogle Scholar
- Unemo M, Dillon JA: Review and international recommendation of methods for typing Neisseria gonorrhoeae isolates and their implications for improved knowledge of gonococcal epidemiology, treatment, and biology. Clin Microbiol Rev. 2011, 24: 447-458.View ArticlePubMedPubMed CentralGoogle Scholar
- Unemo M, Sjöstrand A, Akhras M, Gharizadeh B, Lindbäck E, Pourmand N, Wretlind B, Fredlund H: Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strain. APMIS. 2007, 115: 231-241.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/454/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.