The impact of maternal HIV infection on cord blood lymphocyte subsets and cytokine profile in exposed non-infected newborns
© Borges-Almeida et al; licensee BioMed Central Ltd. 2011
Received: 26 March 2010
Accepted: 3 February 2011
Published: 3 February 2011
Children born to HIV+ mothers are exposed intra-utero to several drugs and cytokines that can modify the developing immune system, and influence the newborn's immune response to infections and vaccines. We analyzed the relation between the distribution of cord blood lymphocyte subsets and cytokine profile in term newborns of HIV+ mothers using HAART during pregnancy and compared them to normal newborns.
In a prospective, controlled study, 36 mother-child pairs from HIV+ mothers and 15 HIV-uninfected mothers were studied. Hematological features and cytokine profiles of mothers at 35 weeks of pregnancy were examined. Maternal and cord lymphocyte subsets as well as B-cell maturation in cord blood were analyzed by flow cytometry. The non-stimulated, as well as BCG- and PHA-stimulated production of IL2, IL4, IL7, IL10, IL12, IFN-γ and TNF-alpha in mononuclear cell cultures from mothers and infants were quantified using ELISA.
After one year follow-up none of the exposed infants became seropositive for HIV. An increase in B lymphocytes, especially the CD19/CD5+ ones, was observed in cord blood of HIV-exposed newborns. Children of HIV+ hard drug using mothers had also an increase of immature B-cells. Cord blood mononuclear cells of HIV-exposed newborns produced less IL-4 and IL-7 and more IL-10 and IFN-γ in culture than those of uninfected mothers. Cytokine values in supernatants were similar in infants and their mothers except for IFN-γ and TNF-alpha that were higher in HIV+ mothers, especially in drug abusing ones. Cord blood CD19/CD5+ lymphocytes showed a positive correlation with cord IL-7 and IL-10. A higher maternal age and smoking was associated with a decrease of cord blood CD4+ cells.
in uninfected infants born to HIV+ women, several immunological abnormalities were found, related to the residual maternal immune changes induced by the HIV infection and those associated with antiretroviral treatment. Maternal smoking was associated to changes in cord CD3/CD4 lymphocytes and maternal hard drug abuse was associated with more pronounced changes in the cord B cell line.
HIV infection is associated with a complex pattern of changes in the hemopoietic and the immune systems, resulting in abnormalities of peripheral blood (PB) counts and changes in T and B lymphocytes. Decrease of T helper and increase of cytotoxic lymphocytes, profound changes in the cytokine profile and a variety of B lymphocyte abnormalities have been repeatedly described [1–4]. However, long term antiretroviral therapy (ARV) is able to restore, at least in part, the immune function [3–6].
The introduction of ARV therapy in HIV+ pregnant women has drastically decreased vertical transmission of HIV [1–7]. But, several changes in PB counts and T CD4+ and CD8+ lymphocytes have been detected in HIV-exposed uninfected newborns [1, 2, 4] and attributed to alterations in maternal cytokine profile caused by the HIV infection as well as by the ARV treatment [2, 8–14]. PB count changes are soon reversed, but some T lymphocyte changes may last for as long as 8 years . Therefore, these infants present an increased risk for severe infections. This risk is further increased as newborns from HIV-infected mothers usually do not receive breast feeding in order to avoid vertical transmission. Changes in T lymphocytes may also affect the response to vaccines given in the neonatal period [2, 10–13].
Alterations in infants' T lymphocyte subsets have been well studied, but little is known about the impact of HIV infection and highly active antiretroviral treatment (HAART) on neonatal maturation and function of B lymphocytes [8, 15].
The aim of our study was to analyze the B cell maturation in umbilical cord blood of infants born to HIV-infected mothers using HAART. We also studied the relation between the distribution of lymphocyte subsets and cytokine production in short term cultures of cord blood mononuclear cells in as well as in maternal peripheral blood mononuclear cells at 35 weeks of pregnancy. We also looked for the relation between maternal smoking and use of hard drugs during pregnancy and infant's lymphocyte subpopulations.
We studied 36 mother-child pairs of HIV positive pregnant women attended at our High Risk Obstetric Unit. They were >18 years old, were using HAART during gestation and had a low or undetectable viral load. All of them had a term delivery. None of the newborns had a malformation at birth. Their data were compared to 15 normal mother-child pairs, which were also attended at our Institution. Mothers of the control group were also >18 years old, had no known pathological condition: hypertension, diabetes, obesity, autoimmune disorders, infections, nor a past history of repeated infections suggesting an underlying immunodeficiency, and had a normal term delivery.
Peripheral blood counts as well as cytokine profile of both groups of mothers as well as viral load of the HIV+ ones were taken between 32 to 35 weeks of gestation.
After 13-17 months of observation none of the exposed infants developed HIV infection. Infants were considered HIV- uninfected and defined as seroreverters if they had negative HIV- polymerase chain reaction (PCR) tests at 1 and 3 months of age or became HIV-seronegative after 13 months of age.
Cord blood analysis
Cytokine assays were performed in supernatants from cultures of peripheral blood mononuclear cells (PBMC) of the mothers at 35 weeks of gestation, and infants' cord blood mononuclear cells (CBMC). In both cases, mononuclear cells were isolated by Ficoll-Hypaque 1077 (Sigma, MO, USA). They were suspended in RPMI 1640 with 10% human serum, 1% glutamine and 0.1% gentamicine and cultured for 48 hours with live Bacillus Calmette-Guérin (BCG) (5 × 105/mL), (Butantan Institute, SP), phytohemagglutinin (PHA, 7.5 μg/mL) (Sigma, MO, USA) or in medium alone (non-stimulated).
Cytokines were quantified by enzyme-linked immunosorbent assay (ELISA) in cell-free supernatants using commercial kits for human IL-2, IL-4, IL-7, IL-10, IL-12, TNF-α and IFN-γ (Duo Set®, R&D Systems Inc, Minneapolis, MN, USA). These cytokines were chosen as they are able to analyze Th1 and Th2 status .
Features of the normal and HIV-exposed newborns and their mothers were compared by the Mann-Whitney and Kruskall-Wallis tests. Values were considered statistically significant when the two-sided p-value was < 0.05. The relationships among the features were examined by the Spearman rank order correlation test. In order to find out which variables were related to birth weight and the T and B cell subsets, multiple step-wise linear regressions were run (0.05 for input and p = 0.1 for output, backward conditional stepwise selection) using the variables showing significant differences or correlations in the univariate analysis. Moreover, the R2 values of each regression, which represent the goodness-of-fit of the mathematical model, were recorded in order to estimate the approximation of the algorithm to clinical and biological reality. Finally, the stability of the model was tested by bootstrap resampling. In this technique we create new data sets of equal size by random sampling of the original data with replacement [16–18]. Thus, in a new bootstrap sample, a patient may be represented once, multiple times or not at all. Then, for each of these "new" sets, a linear regression is calculated and the variables entering each of the "new" models are recorded. This procedure tests the stability of a mathematical model, points out the most important variables and, furthermore, permits to calculate confidence intervals (IC). WinStat 3.1 software was used.
All mothers were invited to participate, received a complete explanation about the aims of the study and written informed consent was obtained according to our local Ethics's Committees (CAISM - Center of Integrated Care for Women's Health, and that of our Faculty of Medicine).
Epidemiologic characteristics of the mothers (median and range)
HIV negative (controls)
Age at delivery (years)
29 (18 - 41)
32 (20 - 39)
Weeks of gestation
39.7 (36 - 42)
38.2 (36 - 42)
Drug user only
Smoker and drug user
Non-smoker non-drug user
Days of HAART use
154 (49 - 273)
detectable viral load copies/mL*
496 (54 - 36586)
Peripheral blood features of the mothers at 35 weeks of pregnancy (median and range)
HIV negative mothers
(3.7 - 4.7)
(2.5 - 4.7)
(9.6 - 13.9)
(9.0 - 13.7)
(72 - 94)
(75 - 118)
(3.2 - 12.2)
(3.0 - 15.0)
Total neutrophils (K/μL)
(2.3 - 8.8)
(2.2 - 10.9)
Total lymphocytes (K/μL)
(0.6 - 2.7)
(0.5 - 4.8)
(57 - 1189)
(321 - 1979)
(0.06 - 1.97)
(102 - 392)
(99 - 360)
Cytokine levels (in pg/ml, median and range) without antigenic stimulus and after stimulation with BCG and PHA in the supernatant from mothers' peripheral mononuclear cell cultures collected at 35 weeks of gestation
0.0 (0 -76)
0.0 (0 - 767)
0.0 (0 - 78)
2.14 (0 - 232)
89.8 (0 -968)
24.5 (0 - 1421)
41.0 (38 - 278)
22.2 (9.2 - 76)
39.1 (37 - 216)
22.8 (9.5 - 76)
52.0 (39 - 146)
30.14 (14 - 419)
67.0 (0 -88)
0.0 (0 - 90)
66.0 (0 - 89)
0.0 (0 - 86)
66.0 (0 - 85)
0.0 (0 - 87)
0.0 (0 - 490)
17 (0 - 18015)
736 (0 - 4910)
764 (0 - 43384)
498 (0 - 2925)
405 (0 - 58344)
0.51 (0 - 2.83)
0.51 (0 -2.4)
1.18 (1 - 31)
1.0 (0 - 11)
1.34 (0 - 51)
1.53 (0 - 33)
0.0 (0 - 94)
13.2 (0 - 131)
2.73 (0 - 1343)
29.2 (0 - 999)
110.2 (0 - 2528)
308.9 (0 - 5143)
245.4 (0 - 4826)
526.8 (0 - 7928)
5374.0 (0 - 13852)
2072.7 (86 - 34130)
827.6 (261 - 20889)
1513.3 (244 - 14448)
The CD4/CD8 ratio in PB of HIV+ mothers showed an inverse relation with viral load (r = -0.30; p = 0.02) and production of TNFα by PBMCs (non-stimulated) (r = -0.27; p = 0.04) and a direct correlation with IL-10 (r = 0.35; p = 0.01). There was no correlation with IL-7.
In a multiple regression, we obtained the formula:
Maternal CD4/CD8 ratio = 0.946 + 5.6 × 10-5 × IL-10 - 9.8 × 10-5 × TNFα - 0.0567 × log viral load + 0.9468 R2 = 0.335 ; p = 0.004
The stability of the model was confirmed in a bootstrap resampling procedure. Among 100 new models, "log viral load" entered in 97%, "IL-10" level in 89%, "TNFα" in 60% and "IL-7" in 28% of them.
Birth data, cord blood counts and lymphocyte subsets of the newborns (median and range)
Not exposed (controls)
HIV - exposed
(2988 - 3915)
(1885 - 3885)
(40 - 53)
(33 - 53)
(256 - 294)
(255 - 293)
(4.1 - 5.0)
(2.7 - 4.7)
(13.2 - 18.0)
(9.1 - 16.4)
(99 - 124)
(88 - 150)
(119 - 328)
(103 - 455)
(4.4 - 19.5)
(4.0 - 22.0)
Total neutrophils (K/μL)
(0.8 - 9.4)
(1.0 - 8.8)
Total lymphocytes (K/μL)
(1.5 - 10.5)
(13.8 - 32.3)
(12.0 - 41.5)
(8.9 - 23.3)
(3.0 - 28.2)
(1.8 - 10.7)
(2.3 - 15.5)
(1.6 - 8.3)
(0.2 - 6.5)
(2.0 - 5.9)
(1.7 - 16.2)
% CD19+CD5+ **
(42.8 - 91.1)
(47.7 - 97.0)
The birth weight of HIV-exposed infants showed a relation with the duration of maternal HAART treatment during pregnancy and the TNFα production (non-stimulated cultures) in supernatants of cultures of PB mononuclear cells collected at the end of gestation. Maternal age, smoking, weeks of gestation and child's gender had no relation to birth weight.
In a multiple regression we obtained the formula:
Birth weight = 3327 - 1.48 × days of HAART - 5 × unstimulated maternal TNFα p = 0.006, R2 = 0.216 ( CI 95% : 0.215 - 0.383 )
The bootstrap resampling study confirmed this result: "days of HAART treatment" appeared in 77% and "non-stimulated maternal TNFα at 35 weeks of gestation" was present in 70% of the new data sets. But, "weeks of gestation" appeared in 40%, "maternal smoking" in 38%, "mother's age" in 12% and "child's gender" in 10% of the new data sets.
The distribution of the several lymphocyte subsets in cord blood is shown on Table 4. The percentage of CD3+ lymphocytes in HIV-exposed newborns was increased when compared with the non-exposed ones. The CD4/CD8 ratio was significantly lower.
In HIV-exposed neonates the relation of the number of cord T lymphocytes with maternal age, days of HAART use during pregnancy, smoking and drug abuse was examined in a multiple regression. For T CD8+ cell no model could be obtained.
The percentage of T CD4+ cells was lower in children from older and smoking mothers.
This percentage could be calculated by the formula:
Cord % T CD4+ cells = -0.428 × mother's age (years) - 4.91 (S) + 30.52;
S = 1 for smoking and 0 for non-smoking mother.
R2 = 0.242 (CI 95%: 16.6 - 51.1)
Drug abuse and days of maternal HAART treatment during pregnancy did not enter the model. The stability of the model for cord blood T CD4+ cells was confirmed in the bootstrap resampling. Among 100 new models, "maternal age" entered in 87% and "maternal smoking" in 72% of them. "Duration of maternal HAART treatment" entered in 42%, and "drug abuse" in 11% of the models.
Percentages of CD4+ and CD8+ blastic cells determined by flow cytometry in CBMCa cultures incubated with PHAb from 22 newborns from HIV+ mothers and 10 HIV negative controls (median and range)
Non exposed controls
B lymphocyte subsets and B-cell maturation in cord blood studied in the CD45/CD34/CD19/CD22 and sIgM/CD34/CD19/CD10 combinations (median and range)
Non exposed controls
HIV exposed drug using mother
% CD19+CD5+ *
% CD45lowCD34+CD19+CD22-/+ **
(0.2 - 3.13)
(0.07 - 1.22)
(0.78 - 1.4)
% CD45-/+CD34lowCD19+CD22+ ***
(0.24 - 6.8)
(1.5 - 8.2)
% CD45+CD34-CD19+CD22+ ****
(91 - 99)
(91.6 - 98.4)
(79 - 90)
B-cell maturation was analyzed in two four-color combinations (Table 6). The CD45/CD34/CD19/CD22 combination was the most useful to reveal an increase in immature and intermediary B-precursors in infants of HIV+ mothers. This feature was more pronounced in children of drug users.
In HIV-exposed neonates, there was no significant difference in the proportion of immature cells with the newborn's gender (p = 0.33) or children of smokers and non-smokers (p = 0.21), but those of drug users showed a higher percentage (p = 0.007) (Table 6). There was no significant correlation between this variable and gestation duration or maternal age but with the number of days of maternal HAART use (r = 0.53; p = 0.01).
In a multiple regression, examining the relation between the percentage of cord blood immature cells with maternal drug abuse and the number of days of HAART treatment during pregnancy, the proportion of immature/intermediate (IMAT) B cells could be calculated by the formula:
% Cord IMAT B cells = 0.02 × days of HAART + B + 0.72; R2 = 0.859 (CI - 0.653 - 0.943)
With B = 12.0 for drug abusers and B = 0 for all other mothers.
The stability of this regression model was tested by the bootstrap resampling study, where both variables entered in 98% of the new data sets.
Cytokine levels (in pg/ml, median and range) without antigenic stimulus and after stimulation with BCG and PHA in the supernatant of cord blood cell cultures
0.0 (0 -76)
0.72 (0 - 81)
0.0 (0 -78)
0.36 (0 - 79)
43.7 (0 - 1278)
18.9 (0 - 5936)
39.2 (39 - 76)
25.5 (10 - 76)
39.4 (40 - 76)
25.6 (9 - 76)
39.2 (39 - 78)
26.7 (9.5 - 76)
66.0 (0 - 87)
0.0 (0 - 87)
66.4 (0 - 87)
1.5 (0 - 93)
66.5 (0 - 87)
0.0 (0.0 - 88)
0 (0 - 88)
3.5 (0 - 1028)
199 (0 - 1522)
582 (0 - 7049)
224 (0 - 1934)
410 (0 - 36093)
0.66 (0 - 13.3)
0.24 (0 - 5.7)
0.83 (0 - 26)
0.74 (0 - 3.64)
0.62 (0 - 3)
1.1 (0 - 8)
0.0 (0 - 66)
17.5 (0 - 194)
0.0 (0 - 1188)
19.4 (0 - 550)
24.3 (0 - 2445)
73.2 (0 - 2928)
136.4 (0 - 500)
181.6 (0 - 3381)
1458.1 (138 - 7026)
1069.0 (283 -14724)
535.0 (298 - 5672)
658.4 (0 - 6313)
Concerning TNFα, HIV+ mothers had a higher production, especially drug users (p = 0.01). CBMCs of HIV-exposed infants produced similar amounts of TNFα but more IFNγ than the control group. No correlation was found between the number of days of maternal HAART treatment during pregnancy and CBMC cytokine production.
Correlations found in HIV-exposed neonates between cord blood B-cell subsets and cytokine production by cord mononuclear cells in culture
r = - 0.49; p = 0.001
r = 0.25; p = 0.07
r = - 0.46; p = 0.002
r = - 0.40; p = 0.008
r = - 0.46; p = 0.002
r = 0.32; p = 0.02
r = - 0.39; p = 0.01
r = 0.41; p = 0.006
r = - 0.48; p = 0.001
r = 0.45; p = 0.003
r = - 0.31; p = 0.05**
r = 0.33; p = 0.02
r = - 0.48; p = 0.02**
r = 0.51; p < 0.0005
r = - 0.39; p = 0.04**
r = 0.41; p = 0.03
A positive correlation was seen between CD19+/CD5+ cells and cord IL-7 and IL-10, but no correlation was found between these cells and production of IL-4, IFNγ and TNFα by CBMCs.
HIV-exposed uninfected infants present a variety of small but significant alterations compared to non-exposed neonates such as a lower birth weight, peripheral blood cytopenias and distribution pattern of circulating lymphocyte subsets [1, 2, 4, 5, 10, 19–25]. Their lower birth weight has been attributed to an earlier delivery due to a higher rate of elective cesarean section  or to an earlier maternal Th2 to Th1 shift related to the antretroviral treatment leading to a premature delivery . In our study however, the gestation period was similar in both groups despite that HIV+ mothers delivered by an elective cesarean section. Low weight at birth of HIV-exposed infants was associated to a high maternal production of TNFα at the end of pregnancy, which was more common in drug abusing mothers. It has been shown that neither HAART nor decreased IL-10 levels, but only increase in maternal IL-2 is associated to a premature delivery [26–28]. To the best of our knowledge there are no data in the literature about the relation between maternal IL-7 and IL-4 and low birth weight and prematurity. In our study, a better control of the maternal HIV infection, expressed by an increase of the CD4/CD8 ratio, was also associated with a lower production of TNFα and higher one of IL-10.
In our prospective controlled trial we compared hematological and immunological features of term newborns from HIV+ women under effective HAART treatment during pregnancy with normal unexposed newborns. Hemoglobin, leukocyte and neutrophil counts were lower, and MCV was higher. These alterations were similar to those described in the literature [1, 2, 4, 10], and could be readily explained by maternal HAART treatment and placental drug transfer, as it has been already pointed out [4, 25]. We could not demonstrate an association of the intensity of the newborns' peripheral cytopenias with the duration of maternal HAART treatment, but when maternal and newborns' PB counts were compared, RBC and MCV showed a significant correlation. We could not find any correlation concerning leukocytes and platelets. Our study cohort was rather small, but other factors, such as maternal nutritional status or use of antibacterial medications could have influenced the PB counts found.
We could also detect several differences in cord blood lymphocyte sub-populations in our HIV-exposed uninfected newborns when compared to children from HIV negative mothers. We studied the possible association of these alterations with the maternal and cord blood cytokine profile, duration of maternal HAART treatment as well as maternal smoking and drug abuse.
Concerning maternal cytokine profile, HIV+ women only differed from uninfected ones by a lower production of IL-4 and IL-7 in culture, but more TNFα, especially in drug using women. A decrease in the Th2 cytokine profile has been described at the end of pregnancy . On the other hand, antiretroviral drugs, especially protease inhibitors as used in the present study, have been associated to a metabolic syndrome associated with an activation of pro-inflammatory cytokines, mainly TNFα . We may speculate that the co-occurrence of maternal HIV-1 infection, use of HAART and hard drugs, all contributed to a higher stimulation of maternal immune cells leading to a larger baseline production of TNF-α in short term culture. The reduced production of IL-4 and IL-7 observed in HIV-infected mothers is in keeping with a change in T-lymphocyte homeostasis in HIV-infected patients.
The decrease of IL-4 and increase of IFN-γ in HIV-exposed uninfected newborns speaks in favor of an environment with a Th1-shifted balance. This is more pronounced in newborns of HIV+ mothers under effective HAART [4, 6, 11, 12]. This treatment has been associated with an at least a partial restoration of the immune function in HIV infected children and adult patients [19–22].
Our results confirmed previous findings of changes in T-cell development in cord blood of uninfected newborns of HIV - infected mothers with undetectable or low viral load [2, 9–11]. Our HIV-exposed uninfected newborns presented higher numbers of CD3+ cells, due mainly to increase in CD8+ lymphocytes as is seen in HIV-infected adults. Lower proportions of CD4+, but not of CD8+ T cells was also associated to a higher maternal age and smoking. Although we examined only a small number of patients, these findings are in keeping with those found in adult smokers [29, 30]. Therefore, not only the maternal HIV infection could alter the thymus function of exposed infants, but also maternal smoking.
Placental transfer of HIV-related proteins and residual maternal immune alterations could be responsible for the findings concerning the immune function of our HIV-exposed non-infected neonates [31, 32]. On the other hand, 11/15 of the healthy control mothers in our study had a normal vaginal delivery, while all except one of the HIV-infected mothers had a cesarean section. Therefore, some differences found between HIV-exposed and non-exposed neonates may be explained by the different mode of delivery of both groups of newborns. It has been shown that normal labor induces immune stimulation in mothers and neonates including a higher production of TNF-α . This could explain the difference found of this cytokine in supernatants of maternal but not in cord mononuclear cultures, as normal newborns were stimulated by labor and HIV-exposed ones were already stimulated by the maternal HIV infection.
The long term CBMC cultures demonstrated similar numbers of T-derived activated cells after PHA stimulation in HIV-exposed neonates and non-exposed ones. Similar proportions of CD4+ and CD8+ cells were also observed in HIV-exposed uninfected newborns and unexposed ones, speaking in favor of a preserved function of these cells.
Cord blood B lymphocytes were increased in HIV-exposed neonates, mainly caused by an increase in CD19+/CD5+ cells. A B-cell dysfunction with appearance of immature/transitional B-cells and hypergammaglobulinemia has also been described in active HIV infection in adults [8, 20, 34–36]. There are some discrepancies in the literature concerning the change in number of these cells in adult HIV-infected patients [19–24]. However, long-term response to HAART seems to restore at least partially, the number and function of these cells [8, 10, 12, 19, 20]. In the present study, the number of CD5+ B lymphocytes was correlated directly with production of IL-7 and IL-10 by CBMCs. CD5 is a negative regulator of the B-cell receptor signaling contributing to a prolonged cell survival and maintaining tolerance in anergic B cells in vivo . In cord blood, CD5+ B lymphocytes represent about half of the B cells . The increase of CD5+ B lymphocytes in our HIV-exposed newborns may be an expression of an immune deregulation due to placental transfer of viral proteins and cytokines. How these changes may affect the neonatal development of HIV-exposed uninfected children is not known, but a reduced response to neonatal vaccines has been reported . So, the changes found in our HIV-exposed newborns indicate that, although HAART controlled maternal HIV infection, leading to low or undetectable viral load at 35 weeks of pregnancy, children still had changes in B lymphocytes, consistent with some remaining HIV-mediated immune abnormalities.
We studied B-cell maturation in cord blood using four-color-antibody combinations proposed by van Lochen et al . Only the combinations that examined the initial maturation of the B cell line were able to disclose differences between normal newborns and those born to HIV+ mothers. Especially maternal drug abuse and a longer period of maternal HAART treatment during pregnancy were associated with a larger number of immature B-cells. This could be a sign of a higher fetal burden of HIV-related proteins and TNFα . Little is known about the influence of cocaine on the immune system, although some earlier studies  have shown a smaller stimulation of cord lymphocytes by PHA and PMA and lower levels of IL-1 and IL-2 in cord blood from newborns of drug abusing mothers.
We conclude that in our study setting, uninfected infants born to HIV+ women, several immunological abnormalities could be detected. They were related to the residual maternal immune changes caused by the HIV infection as well as the use of antiretroviral drugs during pregnancy. Maternal smoking was associated to changes in cord CD3/CD4 cells, and maternal hard drug abuse was associated to more pronounced changes in the cord B cell line. Immunological changes in uninfected infants born to HIV+ women may persist for several years [2, 9–11]. Little is known about their long term clinical significance. It would be interesting to examine in detail the evolution of the PB lymphocyte subsets in our study cohort. The role of maternal smoking and drug abuse on the development of the immune system of their offspring should also be studied in larger cohorts of mother-child pairs.
This work was supported by a grant from FAPESP (São Paulo Research Foundation - project 2006/52596-0) and a grant from the National Council of Technological and Scientific Development (CNPq project 304586/2006-4). ILM and KM have a reseach grant from CNPq. We thank the colleagues of the Obstetric Unit (Dr Eliana Martorano Amaral) and the Neonatal Unit (Dr Sérgio Tadeu Martins Marba) of the CAISM (Center of Integrated Care for Women's Health) University of Campinas for kind support in the care of the patients.
The results presented in this publication are an essential part of the PhD thesis of EBA, supervised by ILM. (Postgraduate Course in Medical Pathophysiology, University of Campinas)
- Blanche S, Rouzioux C, Moscato MLG, Veber F, Mayaux MJ, Jacomet C, Joëlle Tricoire J, Deville A, Vial M, Firtion G, Crepy A, Douard D, Robin M, Courpotin C, Ciraru-Vigneron N, Deist F, Griscelli C, the HIV Infection in Newborns French Collaborative Study Group: A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. N Engl J Med. 1989, 320: 1643-1648. 10.1056/NEJM198906223202502.View ArticlePubMedGoogle Scholar
- Clerici M, Saresella M, Colombo F, Fossati S, Sala N, Bricalli D, Villa ML, Ferrante P, Dally L, Vigano A: T-lymphocyte maturation abnormalities in uninfected newborns and children with vertical exposure to HIV. Blood. 2000, 96: 3866-3871.PubMedGoogle Scholar
- Müller F, Tjonnfjord GE, Nordoy I, Kvale D, Mellbye OJ, Aukrust R, Froland SS: Immunophenotypic analyses of CD34+ cell subsets in bone marrow from HIV-infected patients during highly-active antiretroviral therapy. Eur J Clin Invest. 2002, 32: 535-540.View ArticlePubMedGoogle Scholar
- Pacheco SE, McIntosh K, Ming L, Mofenson LM, Diaz C, Foca M, Frederick M, Handelsman E, Hayani K, Shearer WT, Women and Infants Transmission Study: Effect of perinatal antiretroviral drug exposure on hematologic values in HIV-uninfected children: an analysis of the women and infants transmission study. J Infect Dis. 2006, 194: 1089-1097. 10.1086/507645.View ArticlePubMedGoogle Scholar
- Newell ML, Huang S, Fiore S, Thorne C, Mandelbrot L, Sullivan JL, Maupin R, Delke I, Watts DH, Gelber RD, Cunningham CK, PACTG 316 Study Team: Characteristics and management of HIV-1-infected pregnant women enrolled in a randomised trial: differences between Europe and the USA. BMC Infect Dis. 2007, 7: 60-10.1186/1471-2334-7-60.View ArticlePubMedPubMed CentralGoogle Scholar
- Sachdeva N, Oshima K, Cotter A, Ashman M, Davila L, Okazaki T, Inaba N, Asthana D: Analysis of immunological markers associated with pregnancy and HIV-1 infection: relevance in perinatal transmission in HIV-1-infected pregnant women with low plasma viral load. Am J Reprod Immunol. 2008, 60: 264-273. 10.1111/j.1600-0897.2008.00627.x.View ArticlePubMedGoogle Scholar
- Thorne C, Semenenko I, Pilipenko T, Malyuta R, Ukraine European Collaborative Study Group: Progress in prevention of mother-to-child transmission of HIV infection in Ukraine: results from a birth cohort study. BMC Infect Dis. 2009, 9: 40-10.1186/1471-2334-9-40.View ArticlePubMedPubMed CentralGoogle Scholar
- Feola DJ, Garvy BA: Combination exposure to zidovudine plus sulfamethoxazole-trimethoprim diminishes B lymphocyte immune responses to Pneumocystis murina infection in healthy mice. Clin Vaccine Immunol. 2006, 13: 193-201. 10.1128/CVI.13.2.193-201.2006.View ArticlePubMedPubMed CentralGoogle Scholar
- Nielsen SD, Jeppesen DL, Kolte L, Clark DR, Sørensen TU, Dreves AM, Ersbøll AK, Ryder LP, Valerius NH, Nielsen JO: Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood. 2001, 98: 398-404. 10.1182/blood.V98.2.398.View ArticlePubMedGoogle Scholar
- Ono E, Nunes dos Santos AM, Succi RCM, Machado DM, de Angelis DSA, Salomão R, Kallás EG, de Moraes-Pinto MI: Imbalance of naïve and memory T lymphocytes with sustained high cellular activation during the first year of life from uninfected children born to HIV-1-infected mothers on HAART. Braz J Med Biol Res. 2008, 41: 700-708. 10.1590/S0100-879X2008000800011.View ArticlePubMedGoogle Scholar
- Bunders M, Thorne C, Newell ML, the European Collaborative Study: Maternal and infant factors and lymphocyte, CD4 and CD8 cell counts in uninfected children of HIV-1-infected mothers. AIDS. 2005, 19: 1071-1079. 10.1097/01.aids.0000174454.63250.22.View ArticlePubMedGoogle Scholar
- Zaccarelli-Filho CA, Ono E, Machado DM, Brunialti M, Succi RCM, Salomão R, Kallás EG, de Moraes-Pinto MI: HIV-1-infected children on HAART: immunologic features of three different levels of viral suppression. Cytometry B. 2007, 72: 14-21.View ArticleGoogle Scholar
- Carniel EF, Morcillo AM, Blotta MH, Da Silva MTN, Mazzola TN, Antonio MA, Zanolli ML, Netto AA, Higashi HG, Raw I, Vilela MM: Immunogenicity and safety of combined intradermal recombinant hepatitis B with BCG vaccines at birth. Vaccine. 2008, 26: 647-652. 10.1016/j.vaccine.2007.11.048.View ArticlePubMedGoogle Scholar
- Shearer WT, Rosenblatt HM, Gelman RS, Oyomopito R, Plaeger S, Sthiem ER, Wara DW, Douglas SD, Luzuriaga K, McFarland EJ, Yogev R, Rathore MH, Levy W, Graham BL, Spector SA, Pediatric AIDS Clinical Trials Group: Lymphocyte subsets in healthy children from birth through 18 years of age: the pediatrics AIDS Clinical Trials Group P1009 study. J Allergy Clin Immunol. 2003, 112: 973-980. 10.1016/j.jaci.2003.07.003.View ArticlePubMedGoogle Scholar
- Fiore S, Newell ML, Trabattoni D, Thorne C, Gray L, Savasi V, Tibaldi C, Ferrazzi E, Clerici M: Antiretroviral therapy-associated modulation of Th1 and Th2 immune responses in HIV-infected pregnant women. J Reprod Immunol. 2006, 70: 143-150. 10.1016/j.jri.2005.12.001.View ArticlePubMedGoogle Scholar
- Adam RL, Silva RC, Pereira FG, Leite NJ, Lorand-Metze I, Metze K: The fractal dimension of nuclear chromatin as a prognostic factor in acute precursor B lymphoblastic leukemia. Cell Oncol. 2006, 28: 55-59.PubMedPubMed CentralGoogle Scholar
- Rybka MO, Cintra ML, de Souza EM, Metze K: Density of dendritic cells around basal cell carcinomas is related to tumor size, anatomical site and stromal characteristics, and might be responsible for the response to topical therapy. Int J Dermatol. 2008, 47: 1240-4. 10.1111/j.1365-4632.2008.03808.x.View ArticlePubMedGoogle Scholar
- Lorand-Metze I, Meira DG, Lima CS, Vassallo J, Metze K: The differential diagnosis between aplastic anemia and hypocellular myelodysplasia in patients with pancytopenia. Haematologica. 1999, 84: 564-565.PubMedGoogle Scholar
- Faye A, Pornprasert S, Mary JY, Dolcini G, Derrien M, Barré-Sinoussi F, Chaouat G, Menu E, the ANRS 1267 study team and the HIV-1 PMTCT-PlaNet: Characterization of the main placental cytokine profiles from HIV-1-infected pregnant women treated with anti-retroviral drugs in France. Clin Exp Immunol. 2007, 149: 430-439. 10.1111/j.1365-2249.2007.03411.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Hygino J, Lima PG, Filho RGS, Silva AAL, Saramago CSM, Andrade RM, Andrade DM, Andrade AF, Brindeiro R, Tanuri A, Bento CA: Altered immunological reactivity in HIV-1-exposed uninfected neonates. Clin Immunol. 2008, 127: 340-347. 10.1016/j.clim.2008.01.020.View ArticlePubMedGoogle Scholar
- Resino S, Galán R, Correa R, Pajuelo J, Bellón JM, Munoz-Fernandez MA: Homeostatic role of IL7 in HIV-1 infected children on HAART: association with immunological and virological parameters. Acta Paediatr. 2005, 94: 170-177. 10.1080/08035250410020208.View ArticlePubMedGoogle Scholar
- Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G: Decreasing soluble CD30 and increasing IFNγ plasma levels are indicators of effective highly active antiretroviral therapy. AIDS Res Hum Retrov. 2007, 23: 886-890. 10.1089/aid.2006.0228.View ArticleGoogle Scholar
- Redgrave BE, Stone SF, French MAH, Krueger R, James IR, Price P: The effect of combination antiretroviral therapy on CD5 B-cells, B-cell activation and hypergammaglobulinaemia in HIV-1-infected patients. HIV Med. 2005, 6: 307-312. 10.1111/j.1468-1293.2005.00312.x.View ArticlePubMedGoogle Scholar
- Sampaio A, López-Gómez M, Jimenez-Alonso J, Ortiz F, Samaniego F, Garrido F: CD5+ B lymphocytes in HIV infection: relationship to immunological progression of disease. Clin Immunol Immunopathol. 1993, 66: 260-268. 10.1006/clin.1993.1034.View ArticleGoogle Scholar
- Meira DG, Lorand-Metze I, Toro ADC, Silva MTN, Vilela MMS: Bone marrow features in children with HIV infection and peripheral blood cytopenias. J Trop Pediatr. 2005, 51: 114-119. 10.1093/tropej/fmh096.View ArticlePubMedGoogle Scholar
- Halonen M, Lohman IC, Stern DA, Spangenberg A, Anderson D, Mobley S, Ciano K, Peck M, Wright AL: Th1/Th2 patterns and balance in cytokine production in the parents and infants of a large birth cohort. J Immunol. 2009, 182: 3285-3293. 10.4049/jimmunol.0711996.View ArticlePubMedGoogle Scholar
- Barbaro G, Iacobellis G: Metabolic syndrome associated with HIV and highly active antiretroviral therapy. Current Diabetes Reports. 2009, 9: 37-42. 10.1007/s11892-009-0008-7.View ArticlePubMedGoogle Scholar
- Pornprasert S, Mary JY, Faye A, Leechanachai P, Limtrakul A, Rugpao S, Sirivatanapa P, Gomuthbutra V, Matanasaravoot W, Le Coeur S, Lallemant M, Barré-Sinoussi F, Menu E, Ngo-Giang-Huong N, ANRS 1267 study team and HIV-1 PMTCT-PlaNet: Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine. Curr HIV Res. 2009, 7: 211-217. 10.2174/157016209787581472.View ArticlePubMedGoogle Scholar
- Shuter J, Bernstein SL: Cigarette smoking is an independent predictor of non-adherence in HIV-infected individuals receiving highly active antiretroviral therapy. Nicotine&Tobacco Res. 2008, 10: 731-736.Google Scholar
- Wojna V, Robles L, Skolasky RL, Mayo R, Selnes O, de la Torre T, Maldonado E, Nath A, Meléndez LM, Lasalde-Dominicci J: Associations of cigarette smoking with viral immune and cognitive function in human immunodeficiency virus- seropositive women. J Neurovirol. 2007, 13: 561-568. 10.1080/13550280701620747.View ArticlePubMedGoogle Scholar
- De Maria A, Cirillo C, Moretta L: Occurrence of HIV-1 specific cytolitic T cell activity in apparently uninfected children born to HIV-1 infected mothers. J Infect Dis. 1994, 170: 1296-1301.View ArticlePubMedGoogle Scholar
- Kuhn L, Meddows-Taylor S, Gray G, Tiemessen C: Human immunodeficiency virus (HIV)-specific cellular immune responses in newborns exposed to HIV in utero. Clin Infect Dis. 2002, 34: 267-276. 10.1086/338153.View ArticlePubMedGoogle Scholar
- Malamitsi-Puchner A, Protonotariou E, Boutsikou T, Makrakis E, Sarandakou A, Creatsas G: The influence of the mode of delivery on circulating cytokine concentrations in the perinatal period. Early Human Development. 2005, 81: 387-392. 10.1016/j.earlhumdev.2004.10.017.View ArticlePubMedGoogle Scholar
- Malaspina A, Moir S, Ho J, Wang W, Howell ML, O'Shea MA, Roby GA, Rehm CA, Mican JM, Chun TW, Fauci AS: Appearance of immature/transitional B cells in HIV-infected individuals with advanced disease: correlation with increased IL-7. PNAS. 2006, 103: 2262-2267. 10.1073/pnas.0511094103.View ArticlePubMedPubMed CentralGoogle Scholar
- Metze K, Maciel JA: AIDS and Chagas' disease. Neurology. 1993, 43: 447-448.View ArticlePubMedGoogle Scholar
- Viganò A, Zuccotti GV, Pacci M, Erba P, Castelletti E, Giacomet V, Amendola A, Pariani E, Tanzi E, Clerici M: Humoral and cellular response to influenza vaccine in HIV-infected children with full viroimmunologic response to antiretroviral therapy. J Acquir Immune Defic Syndr. 2008, 48: 289-296.View ArticlePubMedGoogle Scholar
- Gary-Gouy H, Harriague J, Bismuth G, Platzer C, Schmitt C, Dalloul AH: Human CD5 promotes B-cell survival through stimulation of autocrine IL-10 production. Blood. 2002, 100: 4537-4543. 10.1182/blood-2002-05-1525.View ArticlePubMedGoogle Scholar
- Durandy A, Thuillier L, Forveille M, Fischer A: Phenotypic characteristics of human newborns' B lymphocytes. J Immunol. 1990, 144: 60-65.PubMedGoogle Scholar
- van Lochen EG, van der Velden VHJ, Wind HK, te Marvelde JG, Westerdaal NAC, van Dongen JJM: Immunophenotypic differentiation of normal hematopoiesis in human bone marrow: reference patterns for age-related changes and disease-induced shifts. Cytometry B. 2004, 60B: 1-13. 10.1002/cyto.b.20008.View ArticleGoogle Scholar
- Karlix J, Behnke M, Davis-Eyler F, Wobie K, Adams V, Freiburger B, Conlon M, Tebbett I: Cocaine Suppresses Fetal Immune System. Ped Res. 1998, 44: 43-46. 10.1203/00006450-199807000-00007.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/11/38/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.