Figure 1
Analysis of B-cell maturation in cord blood of a normal newborn (control group). Expression of CD34 was used to identify immature B-cells. Maturation was studied using the expression of CD45, CD22, CD10 and membrane IgM (sIgM). A) CD45/CD34/CD19/CD22 combination - black: immature cells - CD45lowCD34+CD19+CD22- red: intermediate cells - CD45-/+CD34lowCD19+CD22+ yellow: mature cells - CD45+CD34-CD19+CD22+. B) sIgM/CD34/CD19/CD10 combination - black: immature cells - sIgM-CD34+CD19+CD10-/+ red: intermediate cells - sIgM+CD34-/+CD19+CD10-/+ yellow: mature cells - sIgM+CD34-CD19+CD10-/+.