- Correction
- Open Access
- Published:
Correction to: Abstract-400 Detection of Cytomegalovirus by real time PCR among pediatric patients in different samples’
BMC Infectious Diseases volume 20, Article number: 475 (2020)
Correction to: BMC Infectious Diseases (2020) 20:324
https://doi.org/10.1186/s12879-020-05038-y
Ramya Dharnidhar1, Kousalya V1, Ramya Barani1, Monika Mani1, Krithika Gopalakrishnan1, Padmasani LN2, Rajakumar PS2, Krishnarathinam Kannan3, Padma Srikanth1
1Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India
2Department of Pediatrics, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India
3Department of Oncology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India
After publication of this supplement [1], it was brought to our attention that an author’s name has been erroneously spelled. It should be read “Ramya Dharnidhar’” instead of “amya Dharnidhar’”.
Background: Cytomegalovirus (CMV) is a leading cause of congenital infection resulting in hearing loss and systemic infection. Virus latency and reactivation can cause compartmentalized disease and transient viremia. CMV was reported in urine and saliva among pediatric patients in the absence of CMV viremia. To improve the detection rate of CMV among pediatric patients we used multiple samples to rule out CMV infection by real time PCR.
Methodology: Pediatric patients suspected for CMV infection were included. Both blood/urine/saliva was collected and participants who consented to give more than one samples were included. DNA was extracted and quantitative real time PCR (R-gene kit) was performed targeting ppUL83. The lower detection limit is < 50 copies/ml.
Results: A total of 119 study participants were enrolled. In all, 54% (n = 64) were male 46% (n = 55) female. Samples were collected from infants (n = 24) neonate (n = 48); pediatric (n = 47). About 24% (n = 29) had CMV infection. In single sample testing, CMV was detected in 11 participants, including blood (n = 6), urine (n = 4); saliva (n = 1). In four participants CMV was detected in all three samples. Five participants were found to be positive for CMV in urine only. CMV was detected only in blood in two patients. Five participants were found be positive for CMV in paired sample (B&U-1; B&S-2; U&S-2).
Conclusion: Detection of such infection by real-time PCR using specific gene is lifesaving in children with impaired immunity. Therefore detection of CMV in urine and saliva in first two weeks of life may help to prevent other complications.
Reference
Dharnidhar R, Kousalya V, Barani R, Mani M, Gopalakrishnan K, Padmasani LN, Rajakumar PS, Kannan K, Srikanth P. Abstracts from the International Science Symposium on HIV and Infectious Diseases (ISSHID 2019): Infectious diseases. BMC Infectious Diseases. 2020;20(Suppl 1):324. https://doi.org/10.1186/s12879-020-05038-y.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Correction to: Abstract-400 Detection of Cytomegalovirus by real time PCR among pediatric patients in different samples’. BMC Infect Dis 20, 475 (2020). https://doi.org/10.1186/s12879-020-05119-y
Published:
DOI: https://doi.org/10.1186/s12879-020-05119-y