ISSHID: E-poster Presentations Abstract-5 Drug resistance profile of Gram negative and related exotic bacterial isolates from an urban hospital in Chennai, Tamil Nadu, India
Reenaa Muthu1, Gayathri Devanandan1, Lalitha Kameswari Sankaranarayanan1, Parthiban Rudrapathy2
1Department of Microbiology, Ethiraj College for Women, Chennai, Tamilnadu, India; 2Division of Microbiology, Department of Clinical Laboratory Services and Translational Research, Malabar Cancer Centre, Thalassery, Kerala, India
Background: Despite the impressive range of therapies available for treating patients in intensive care, bacterial infections continue to be the major challenge worldwide. Impact of gram negative organisms on clinical disease is well known but exotic bacterial pathogens such as Ralstonia sp are lesser known members of this group. Infections related with exotic pathogens are often reported in mechanically ventilated patients particularly associated with those who are immunocompromised. This study aims to analyse the gram negative pathogens and their antibiotic susceptibility profile from various clinical and environmental samples (blood, urine, IV and fomites) from an urban multi-speciality hospital in Chennai, Tamilnadu, India using standard protocols and the data discussed.
Methods: Basic microbiological techniques were used to screen the clinical and hospital environmental specimens for gram negative bacteria and their antibiotic susceptibility testing was performed by standard disc diffusion method according to CLSI guidelines. Bacterial isolates which could not be confirmed by basic phenotypic techniques were identified by using automated bacterial identification system (VITEK-2, Biomerieux) and their antibiogram was noted.
Results: A total of 40 clinical samples (39 urine and 1blood) and 60 environmental samples were collected. Among these 16/39 urine samples and 17/60 environmental samples were positive for gram negative bacilli. All the gram negative isolates were resistant to nitrofurantoin used to treat UTI.
Conclusion: Gram negative organisms and exotic gram negative pathogens are most often associated with hospital acquired UTIs. All Ralstonia sp isolated showed resistant pattern for nitrofurantoin, one of the antibiotic commonly used to treat urinary tract infections.