Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China
- Xiaoying Xie†1,
- Yunwen Bao†1,
- Nengyong Ouyang†2,
- Xinlu Dai1,
- Kunyi Pan1,
- Baiji Chen1,
- Yawen Deng1,
- Xiquan Wu1,
- Fengqin Xu1,
- Hongyu Li1Email author and
- Songyin Huang1Email author
© The Author(s). 2016
Received: 26 January 2016
Accepted: 14 June 2016
Published: 22 July 2016
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of both hospital and community infections globally. It’s important to illuminate the differences between community-acquired MRSA (CA-MRSA) and hospital-acquired MRSA (HA-MRSA), but there have been confusions on the definition, especially for the MRSA isolates identified within 48 h of admission. This study aimed to determine the molecular characteristics and virulence genes profile of CA and HA-MRSA isolates identified less than 48 h after hospital admission in our region.
A total 62 MRSA isolates identified within 48 h after admission and the clinical data were collected. Antimicrobial susceptibility test (AST) of collected isolates were performed according to the guidelines of Clinical and Laboratory Standards Institute (CLSI) 2015, and staphylococcal cassette chromosome mec (SCCmec) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and virulence gene profiling were performed to explore the molecular diversity.
SCCmec III and sequence type (ST) 239 were the most prevalent SCCmec type and ST in both CA and HA-MRSA groups. HA-MRSA group had higher prevalence of SCCmec III (87.2 %) and ST239 (79.5 %) compared with CA-MRSA (60.9 and 43.4 %, both P < 0.001), while the frequency of SCCmec IV (26.0 %) and ST59 (21.7 %) were higher in CA-MRSA than its counterpart (P < 0.001 and P = 0.003). MRSA-ST239-III was the predominant type in this study (61.3 %, 38/62), especially in HA-MRSA group (76.9 %, 30/39). However, CA-MRSA strains exhibited more diversity in genotypes in this study. Meanwhile, CA-MRSA tended to have lower resistant percentage to non-β-lactams antibiotics but more virulence genes carriage, especially the staphylococcal enterotoxins (SE) genes. Notably, seb gene was only detected in CA-MRSA isolates (52.2 %), likely a significant marker for CA-MRSA isolates. Panton-Valentine leukocidin gene (PVL) was highly detected in both groups, while appeared no significantly different between CA-MRSA (47.8 %) and HA-MRSA (43.6 %).
Our findings support a difference in the molecular epidemiology and virulence genes profile of CA-MRSA and HA-MRSA. Furthermore, this study indicates a possible transmission from HA-MRSA to CA-MRSA, which may cause the overlap of the definition.
KeywordsMethicillin-resistant Staphylococcus aureus Antimicrobial susceptibility Molecular characteristics Virulence gene
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections around the world since 1960s, first reported in England , and the increasing prevalence rates were noticed by the researchers in recent years [2, 3]. Otherwise, the epidemiology of MRSA infection has changed in the past decades because of the emergence of the strains acquired outsides the healthcare environment named community-associated Staphylococcus aureus (CA-MRSA) . According to the previous reports, CA-MRSA isolates have somewhat differences from hospital-acquired MRSA (HA-MRSA) isolates in molecular characterization, antibiotic resistant pattern, pathogenicity and virulence factors .
As reported, the predominant HA-MRSA clones in China are MRSA-ST239-III and MRSA-ST5-II . In contrast, the main CA-MRSA clones are MRSA-ST59-VI/V and its single locus varietas MRSA-ST338-IV/V. It was suggested that most CA-MRSA isolates carry Staphyloccoccal cassette chromosome mec (SCCmec) types IV and V, do not have multi-antibiotic resistance (except to β lactams) [7, 8]. However, in some studies recently, SCCmec IV was identified in HA-MRSA isolates and SCCmec III was identified in both HA-MRSA and CA-MRSA isolates, showed that the two SCCmec typing isolates may spread between hospital and community in some areas . The overlap of molecular characteristic of CA-MRSA and HA-MRSA was eminently possible in the MRSA isolates which identified within 48 h after admission to hospital [10, 11]. As the main metropolises with a large population of residents and visitors in Southern China, Guangzhou may have an unique transmission pattern of HA-MRSA and CA-MRSA, especially the MRSA strains obtained within 48 h of admission, while rarely studied.
Furthermore, CA-MRSA isolates posses different virulence gene profiles to their counterparts, such as genes, coding a leukocidin well known for its apoptosis induction in human polymorphic leukocytes and its lytic effects in the cells. Since the confusion about definitions of CA-MRSA and HA-MRSA isolates by traditional method basic on clinical and epidemiological criterion, the specific virulence gene (PVL or LukF/lukS-PV) become a new molecular marker [11, 12]. Besides, CA-MRSA was also shown to have higher expression levels of hemolysins and superantigens, such as staphylococcal enterotoxins gene sek and seq, suggesting that they are more virulent than HA-MRSA . It can be supposed that these virulence genes may be new candidates of classified markers, which were few reported as well.
In order to understand the molecular epidemiology and characterization of virulence gene of CA-MRSA and HA-MRSA isolates in this region, 39 HA-MRSA and 23 CA-MRSA isolates were collected from Sun Yat-Sen Memorial Hospital from Jan.1, 2006 to Dec.31, 2011. These isolates were genotyped by SCCmec typing and multilocus sequence typing (MLST). The genetic relationship of the isolates was determined by pulsed-field gel electrophoresis (PFGE). The presence of virulence genes in CA-MRSA and HA-MRSA isolates were determined to establish their possible relationship to clinical outcomes. This knowledge will contribute to our understanding of how the MRSA epidemic has evolved.
Study design and population
Sun Yat-Sen Memorial Hospital is also known as the Second Affiliated Hospital of Sun Yat-Sen University. With more than 4200 staff and 2200 inpatient beds available, the hospital performs over 50,000 inpatient operations, discharges about 80,000 inpatients, and handles more than 3 million outpatient visits annually. A hospital-based retrospect study of 587 inpatients hospitalized for < 48 h infected by Staphylococcus aureus, included 67 inpatients with MRSA infection in Sun Yat-Sen Memorial Hospital between Jan.1, 2006 and Dec.31, 2011 were carried out. Five patients with MRSA infection were eliminated because the strains can’t recovery. Therefore, a total of 62 compete surveys were obtained, with an efficiency rate of 92.5 %. Based on the US Centers for Disease Control and Prevention criteria, we roughly defined HA-MRSA infections as those patients who fit any one of the following situations: surgery, haemodialysis, residence in a long-term care facility or treatment in hospital during the previous year, presence of a permanent catheter or percutaneous device at the time of culture, or previous isolation of MRSA. If the patients did not meet any of the above risk factors, had an infection at the time of admission, and the culture of the infection was taken within 48 h, then the infection was considered as CA-MRSA infections. Clinical data related to the type of infection and outcome (death, improvement, and relapse) of the patients, as well as other data, were collected for up to a period of 30 days after the diagnosis of infection by MRSA. All patients, parents or guardians signed informed consent approving the use of their specimen samples for research purposes and the study was approved by the Ethics Committee of Sun Yat-Sen Memorial Hospital. Ethical committee’s reference number: 伦备第 (11)号 (Additional file 1).
According to the above definition, 39 HA-MRSA isolates and 23 CA-MRSA isolates were collected from the 62 patients. To avoid sample duplication, isolates that were consecutively isolated from the same individual were excluded. All isolates underwent phenotypic identification using the VITEK® 2 microbial identification system (bioMérieux, Marcy l’Etoile, France) according to the manufacturer’s instructions. Antibiotics used for susceptibility testing included penicillin, erythromycin, clindamycin, cefuroxime, ceftriaxone, cefoxitin, gentamicin, rifampicin, quinupristin/dalfopristin, trimethoprim/sulfamethoxazole, cefotaxime, tetracycline, imipenem, teicoplanin, vancomycin, ciprofloxacin, and moxifloxacin. Susceptibilities were determined using the disk diffusion method in accordance with the performance standards for antimicrobial susceptibility testing, 25rd informational supplement (M100-S25), recommended by the Clinical and Laboratory Standards Institute. The inducible clindamycin resistance was determined by D-test. All disks were obtained from Oxoid Ltd (Oxoid, Basingstoke, England), and S. aureus ATCC 25923 and ATCC29213 were used as the quality control strain.
Bacterial DNA was extracted using DNA extracted kit(Tiangen Biotech, Beijing) with lysostaphin according to the manufacturer’s instructions. The sequence type (ST) was characterized by MLST, and the products of seven house-keeping gene fragments were sequenced (Sangon Biotech, Shanghai) and compared with allele profiles from database of S. aureus (www.mlst.net/). SCCmec typing of MRSA strains was conducted by multiplex PCR method as previously described . The genetic relationship of the isolates was determined by PFGE as previously described . Cluster analysis was performed with the software program BioNumerics 5.0 (Maths, Belgium) using the Dice coefficient and the unweighted pair group method (UPGMA). The isolates with the similarity over 75 % were clustered in patterns. The presence of the genes that code for PVL (lukF-PV and lukS-PV), twelve staphylococcal enterotoxins (sea ~ see, seg ~ sej and sem-seo), two exfoliative toxins (eta and etb), four hemolysins (α-hemolysin, β-hemolysin, δ-hemolysin and γ-hemolysin), and the toxic shock syndrome toxin (tsst-1) and mecA gene was determined using PCR as previously reported methodologies, with some modifications [14, 15].
In descriptive statistics, frequency and proportions were calculated for categorical variables. The frequency of SCCmec type, specimen type and virulence genes were treated as categorical variables. The chi-square or two-sided Fisher’s exact test was used to discriminate whether the distributions of the studied genes or types were significantly different between HA-MRSA and CA-MRSA. The only continuous variable, age, was transformed into a categorical variable using the quartiles of the frequency distribution (~18, 19–60, >60). It was considered statistically significant if the two-side P-value < 0.05. All statistical analyses were carried out using SPSS 19.0 for Windows (IBM). All susceptibility data and molecular test results were analyzed using WHONET software, version 5.6.
Clinical and epidemiological data
Clinical features of the HA-MRSA and CA-MRSA strains
(n = 23)
(n = 39)
Type of infection
Infection of skin and soft tissue
Infection of the surgical site
Infection of bone and joint
Death within 1 month
Molecular characteristics of HA-MRSA and CA-MRSA
Multilocus sequence typing of 62 MRSA strains revealed 12 different sequence types. Among these, ST239 and ST59 the most prevalent, accounting for 66.1 and 11.3 % of all isolates, respectively. The prevalence of the ST239 was significantly higher in isolates from HA-MRSA compared with those from CA-MRSA (79.5 % vs. 43.4 %, P < 0.001), whereas the ST59 was more common among isolates from CA-MRSA (21.7 % vs. 5.1 %, P = 0.003). ST45, ST950, ST338, ST7, ST10 and ST398 were only found in the CA-MRSA group; ST537, ST5 and ST8 were found in HA-MRSA group only. Additionally, a new ST clone ST3073 (3-1-1-8-345-1-1) (ID:5611) was found in the HA-MRSA group, obtained from nasal secretion of a 26 years female patients in 2009, who suffered from a nasopharyngeal surgical site infection. Besides, one HA-MRSA was untypable (Additional file 2: Table S3). In short, MRSA-ST239-III were the most prevalent of all isolates (61.3 %, 38/62), which was found in 30 (76.9 %) HA-MRSA isolates and eight (34.8 %) CA-MRSA isolates (Fig. 2 and Additional file 2: Table S4).
Detection of the 20 virulence genes of 62 MRSA isolates in this study
No. Of positive isolates
Virulence genes carriage of S. aureus, n (%)
(n = 62)
(n = 23)
(n = 39)
Among isolates classified as CA-MRSA, the detection rates of sec and seo were higher than HA-MRSA group (P < 0.05) and all the seb positive isolates were CA-MRSA strains (52.2 %, 12/23). On the other hand, seh gene was only found in HA-MRSA isolates (5.1 %, 2/39). Furthermore, 65.2 % CA-MRSA strains carried over than five virulence genes, were obviously higher than that in HA-MRSA group (35.9 %, P < 0.05).
Detection of the virulence genes of MRSA in accordance with the clinical diagnosis of the associated infection
Number and percentage of the MRSA isolates, n(%)
Infection of the surgical site
Infection of skin and soft tissue
(n = 6)
(n = 31)
(n = 24)
Genetic relationship by PFGE
The relationship of molecular characterization, antimicrobial resistance, and the main virulence factor profiles of 62 MRSA isolates
The main virulence factor profiles
sea, PVL, hla,hlb,hlg
seg,sei,sem ~ o,PVL, hla,hlb,hlg
sea(seb),sec,seg(sei),sem ~ o,PVL,hla,hlb,hlg
The combinative analysis of electrophoretic pattern, SCCmec type, sequence type, virulence factor profile and drug resistance pattern suggested different resistance patterns and virulence factor profiles in different PFGE clusters. Combined with the isolates distribution, it indicated that CA-MRSA exhibited great diversity in genetic characteristic and tended to be less resistant to non-β-lactams antibiotics but more virulence genes carried. As the Table 4 showed, the isolates with pattern C, D, E and F, mainly CA-MRSA strains, often were resistant to one or two non-β-lactams antibiotics (TET/E), carried over than three SE genes besides the three hemolysin genes. In contrast, 87.9 % of the isolates with pattern A were MRSA-ST239-III, classified as HA-MRSA, often presented co-resistance to four or more non-β-lactams antibiotics (CIP/CN/E/TET), while only carried one or two SE genes besides the hemolysin genes (Table 4).
The eight PFGE gene clusters further divided into 22 genotypes. Due to the long time span of and different tissue resource of isolates, it indicated that there were not epidemic trend of MRSA strains between 2006 and 2011 in Guangzhou (Additional file 4: Figure S2).
MRSA is an increasing problem and its burden continues to rise in hospital and community setting, leading to increased morbidity and mortality rates. Despite the high prevalence, only a few epidemic clones have been identified in China. As reported, Brazilian or Hungarian clone (ST239) and the New York/Japan clone (ST5) were prevalent in some Asia countries, including China . Previous studies from China found the majority HA-MRSA strains causing bloodstream infection in Shanghai belonged to above two epidemic clones ST239 and ST5 . Wang et al. reported the first molecular characterization of CA-MRSA in 2004, belonged to ST59 . Since then, molecular epidemiology of CA-MRSA in China had aroused more and more concern. During the last decade, ST59 (and its single locus variant ST338) was the major lineage to the CA-MRSA isolates in China [8, 14]. Consistently in this study, ST239 was the predominant clone among HA-MRSA strains, whereas, it is also detected among CA-MRSA. The result showed that 76.9 % (30/39) ST239 MRSA isolates belonged to PFGE cluster A suggesting a similar epidemiology for both hospital acquired infections and community acquired infections.
According to the previous reports , HA-MRSA is usually detected with SCCmec type I, II, and III; in contrast, CA-MRSA is reported as carrying type IV and V. In this study, SCCmec III was the dominant type in HA-MRSA strains (87.3 %), also the main type of CA-MRSA (60.9 %). This study confirms that CA-MRSA clones partially carry the SCCmec type III and SCCmec type I, as reported in a recent study in Guangzhou . In previous studies from the USA  and Italy , SCCmec type IV were also found in HA-MRSA. This finding demonstrates the existence of constant genetic change in not just CA-MRSA but also in HA-MRSA. These bacteria undergo genetic variations that can give rise to isolates with greater capacities for environmental adaption.
It’s widely assumed that there are two sources of CA-MRSA: the spread of HA-MRSA from the medical staff and discharged patients, and the community MSSA strains acquired the resistance gene mecA. MRSA strains colonized in the nasal vestibule of hospital staff and patients can lead the spread in ward . Our recent study also showed that hospital workers had a high risk of MRSA nasal carriage, and the carriers may ‘impose’ their carrier status upon other household members, causing transmission of HA-MRSA strains to the community . In this study, the CA-MRSA-ST239-III strains had a high homology with HA-MRSA, shared the same PFGE cluster (Patterns A and B), similar virulence factor profiles and resistance patterns, indicated the strains’ transmission between healthy-care set and community can lead overlap in the distinction between HA-MRSA and CA-MRSA.
MRSA strains emerged and diffused in the community (CA-MRSA) and were shown frequently to carry the smaller and more easily mobilized SCCmec cassettes and are generally less drug resistant than their counterparts acquired in hospitals (HA-MRSA) . Unexpectedly, except for resistance to all kinds of β-lactam antibiotics, the MRSA isolates found in our region appeared a high resistant rate to other non-β-lactam antibiotics, especially to erythromycin (93.3 %), clindamycin (93.5 %) and tetracycline (74.2 %), mainly in HA-MRSA strains of ST239-III in Pattern A. Unlike the HA-MRSA strains, CA-MRSA strains exhibited great diversity in genetic characteristic in this study. Except for the MRSA-ST239 and ST59, another seven unique sequence type constituted almost half of 23 CA-MRSA strains, belonged to Pattern C ~ H respectively. In comparison with HA-MRSA, these CA-MRSA isolates showed high levels of sensitivity to ciprofloxacin, moxifloxacin, gentamicin, rifampicin, and trimethoprim/sulfamethoxazole. These results are consistent with previous studies [20–22].
To our knowledge, this is the first study to provide insight into the epidemiological and genetic correlation of the virulence profile present among the different CA-MRSA and HA-MRSA clones and their clinical diagnosis in Mainland China. Hemolysin of S. aureus leads the bacterio-lytic enzyme release to damage the nearby organizations through effecting the red blood cell, platelet and neutrophil . α-hemolysin and β-hemolysin are the main toxin inducing pathological injury. Consistent with the report in China , almost all the isolates contained hla, hlb and hlg gene but no isolate was hld positive. Difference from our report, the hla, hlb, hlg and hld were all found in Wenzhou . These findings indicated that the high hla, hlb and hlg genes but low hld gene present in the MRSA strains could be a toxin characteristics of MRSA strains in this region.
In terms of virulence factors, differential content was reported between CA-MRSA and HA-MRSA isolates. This study confirms that CA-MRSA strains carried more virulence genes, especially staphylococcal enterotoxins genes (seb/sec/sem ~ o). SEs are a major cause of staphylococcal food poisoning, associated with severe disease. SEB may suppress the motility of human poly-morphonuclear neutrophils through the inhibition of exoprotein expression, and allow MRSA to invade and damage tissues . Previous studies have confirmed that the sea and seb genes are the most abundant toxin genes in clinical S. aureus isolates from patients in China [26, 27]. Consistently, sea gene was the predominant SE gene detected in this study. No differences between the two MRSA groups were detected nor correlation to the clinical diagnosis. Of note, the seb and sec genes were significantly associated with skin and soft tissue infection (P < 0.001), and proved to be significant markers of CA-MRSA isolates. This study confirms that HA-MRSA clones predominantly carry the egc cluster(sea, seg, sei, sem, sen and seo), as reported in Europe and the USA . Notablely, the tsst-1 gene was only found in the strains of skin and tissue infection patients, suggesting that these patients may have a greater potential for developing fatal toxin shock syndrome (TSS) . Additionally, three SE genes (sed, see and sej) and two ET genes (eta and etb) were not found in this study.
An important finding in this study was the high detection rate of the lukF-PV and lukS-PV gene in the 62 MRSA isolates, while appeared no significantly different between CA-MRSA (47.8 %) and HA-MRSA (43.6 %) groups. PVL is a pore-forming toxin encoded by lukF-PV and lukS-PV, belonging to a family of similar bi-component leukocidin toxins produced by staphylococci, which is postulated widely as the primary virulence determinant driving the epidemic spread of the major CA-MRSA clone worldwide [29, 30]. Although PVL is known as a common virulence factor of CA-MRSA, several studies showed the HA-MRSA isolates had a considerable rate of PVL positivity in their regions . Zetola et al. found that the prevalence of PVL tended to be elevated in nosocomial infections, indicating possible clonal expansion of those CA-MRSA into the hospital environment .
From the above, PVL may no longer be a reliable marker for CA-MRSA isolates, rather all MRSA may be important reservoir of PVL toxin. This indicates that HA-MRSA with typical molecular characteristics of CA-MRSA (SCCmec type IVa, V and PVL positive) have emerged as an important cause of healthcare-associated infections.
The current work has several limitations. Most importantly, the low number of studied strains and the lack of bacteremia cases limited the broad representative significance of the research. Secondly, the protein expression of virulence gene was not studied yet. It was reported that the expression of drug-resistance genes in S. aureus strains could decrease the expression of virulence factors [25, 33], which need further studied. Thirdly, spa typing was not performed in the current study, and this is something that should be conducted in future analyses. Despite the above limitations, our results add to the epidemiological information on CA-MRSA and HA-MRSA, could contribute to more effective disease control of MRSA infections.
These data indicate a high prevalence of ST239-III-MRSA in both CA-MRSA and HA-MRSA isolates identified within 48 h after admission in Sun Yat-Sen Memorial Hospital, Guangzhou, but obviously different characteristic of molecular epidemiology and virulence genes profile between the CA and HA-MRSA group. Furthermore, this study indicates a possible transmission from HA-MRSA to CA-MRSA, that may be useful for understanding the overlap of the definition of the above two, and for designing strategies to prevent the MRSA dissemination.
AST, antimicrobial susceptibility test; CLSI, Clinical and Laboratory Standards Institute; CA-MRSA, community-acquired methicillin-resistant Staphylococcus aureus; ET, exfoliative toxin; HA-MRSA, hospital-acquired methicillin-resistant Staphylococcus aureus; hla ~ hlg, α-hemolysin ~ γ-hemolysin gene; MLST, multilocus sequence typing; MRSA, methicillin-resistant Staphylococcus aureus; PFGE, pulsed-field gel electrophoresis; PVL, Panton-Valentine leukocidin gene ; SE, staphylococcal enterotoxins; ST, sequence type; SCCmec, staphylococcal cassette chromosome mec; sea ~ seo, staphylococcal enterotoxins A ~ O gene; tsst-1, toxic shock syndrome toxin 1 gene.
We are indebted to Professor Bin Huang of the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, and Professor You-Fang Yu of Wenzhou Medical College, Zhejiang, China, for providing the positive control strain used in this study.
This work was supported by grants from the National Natural Science Foundation of China (81172526), the Science and Technology Foundation of the Guangdong Province (2012A032500003, 2014A020212711, 2014A050503029), and the Foundation of Guangzhou Science and Technology Bureau (2014 J4100166, 201400000004–1). This work was also supported by Grant  163 from Key Laboratory of Malignant Tumor Molecular Mechanism and Translational Medicine of Guangzhou Bureau of Science and Information Technology. The funders played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Availability of data and materials
All the data supporting the findings is contained within the manuscript.
SYH, HYL, XYX, YWB and NYO conceived and designed the experiments. XYX, YWB, XLD, BJC, KYP, YWD, XQW, and FQX performed the experiments. XYX, YWB and YWD analyzed data. SYH and XYX wrote the paper. All of the authors read and approved the final version of the manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
All patients, parents or guardians in this study signed informed consent approving the use of their specimen samples for research purposes and the study was approved by the Ethics Committee of Sun Yat-Sen Memorial Hospital. Ethical committee’s reference number:  伦备第(11)号 (Additional file 1).
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- Jevons MP, Coe AW, Parker MT. Methicillin resistance in staphylococci. Lancet. 1963;1:904–7.View ArticlePubMedGoogle Scholar
- Hiramatsu K, Okuma K, Ma XX, Yamamoto M, Hori S, Kapi M. New trends in Staphylococcus aureus infections: glycopeptide resistance in hospital and methicillin resistance in the community. Curr Opin Infect Dis. 2002;15:407–13.View ArticlePubMedGoogle Scholar
- Salgado CD, Farr BM, Calfee DP. Community-acquired methicillin-resistant Staphylococcus aureus: a meta-analysis of prevalence and risk factors. Clin Infect Dis. 2003;36:131–9.View ArticlePubMedGoogle Scholar
- Hsueh PR, Teng LJ, Chen WH, Pan HJ, Chen ML, Chang SC, Luh KT, Lin FY. Increasing prevalence of methicillin-resistant Staphylococcus aureus causing nosocomial infections at a university hospital in Taiwan from 1986 to 2001. Antimicrob Agents Chemother. 2004;48:1361–4.View ArticlePubMedPubMed CentralGoogle Scholar
- David MZ, Cadilla A, Boyle-Vavra S, Daum RS. Replacement of HA-MRSA by CA-MRSA infections at an academic medical center in the midwestern United States, 2004–5 to 2008. PLoS One. 2014;9:e92760.View ArticlePubMedPubMed CentralGoogle Scholar
- Chuang YY, Huang YC. Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Asia. Lancet Infect Dis. 2013;13:698–708.View ArticlePubMedGoogle Scholar
- Du J, Chen C, Ding B, Tu J, Qin Z, Parsons C, Salgado C, Cai Q, Song Y, Bao Q, et al. Molecular characterization and antimicrobial susceptibility of nasal Staphylococcus aureus isolates from a Chinese medical college campus. PLoS One. 2011;6:e27328.View ArticlePubMedPubMed CentralGoogle Scholar
- Geng W, Yang Y, Wu D, Zhang W, Wang C, Shang Y, Zheng Y, Deng L, Fu Z, Li X, et al. Community-acquired, methicillin-resistant Staphylococcus aureus isolated from children with community-onset pneumonia in China. Pediatr Pulmonol. 2010;45:387–94.PubMedGoogle Scholar
- Yao D, Yu FY, Qin ZQ, Chen C, He SS, Chen ZQ, Zhang XQ, Wang LX. Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections (SSTIs). BMC Infect Dis. 2010;10:133.View ArticlePubMedPubMed CentralGoogle Scholar
- Scazzocchio F, Aquilanti L, Tabacchini C, Iebba V, Passariello C. Microbiological and molecular characterization of nosocomial and community Staphylococcus aureus isolates. Epidemiol Infect. 2011;139:613–622.Google Scholar
- Portillo BC, Moreno JE, Yomayusa N, Alvarez CA, Cardozo BE, Perez JA, Diaz PL, Ibanez M, Mendez-Alvarez S, Leal AL, Gomez NV. Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Colombia. Int J Infect Dis. 2013;17:e744-749.Google Scholar
- Gillet Y, Issartel B, Vanhems P, Fournet JC, Lina G, Bes M, Vandenesch F, Piemont Y, Brousse N, Floret D, Etienne J. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet. 2002;359:753–9.View ArticlePubMedGoogle Scholar
- Chen X, Yang HH, Huangfu YC, Wang WK, Liu Y, Ni YX, Han LZ. Molecular epidemiologic analysis of Staphylococcus aureus isolated from four burn centers. Burns. 2012;38:738–42.View ArticlePubMedGoogle Scholar
- Soriano A, Marco F, Martinez JA, Pisos E, Almela M, Dimova VP, Alamo D, Ortega M, Lopez J, Mensa J. Influence of vancomycin minimum inhibitory concentration on the treatment of methicillin-resistant Staphylococcus aureus bacteremia. Clin Infect Dis. 2008;46:193–200.View ArticlePubMedGoogle Scholar
- Diep BA, Chambers HF, Graber CJ, Szumowski JD, Miller LG, Han LL, Chen JH, Lin F, Lin J, Phan TH, et al. Emergence of multidrug-resistant, community-associated, methicillin-resistant Staphylococcus aureus clone USA300 in men who have sex with men. Ann Intern Med. 2008;148:249–57.View ArticlePubMedGoogle Scholar
- Chen X, Wang WK, Han LZ, Liu Y, Zhang H, Tang J, Liu QZ, Huangfu YC, Ni YX. Epidemiological and genetic diversity of Staphylococcus aureus causing bloodstream infection in Shanghai, 2009–2011. PLoS One. 2013;8:e72811.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang CC, Lo WT, Chu ML, Siu LK. Epidemiological typing of community-acquired methicillin-resistant Staphylococcus aureus isolates from children in Taiwan. Clin Infect Dis. 2004;39:481–7.View ArticlePubMedGoogle Scholar
- Lee TM, Yang MC, Yang TF, Lee PL, Chien HI, Hsueh JC, Chang SH, Hsu CH, Chien ST. Molecular Characterization of Community- and Healthcare-Associated Methicillin-Resistant Staphylococcus aureus Isolates in Southern Taiwan. Microb Drug Resist. 2015;21:610–21.View ArticlePubMedGoogle Scholar
- Chen B, Dai X, He B, Pan K, Li H, Liu X, Bao Y, Lao W, Wu X, Yao Y, Huang S. Differences in Staphylococcus aureus nasal carriage and molecular characteristics among community residents and healthcare workers at Sun Yat-Sen University, Guangzhou, Southern China. BMC Infect Dis. 2015;15:303.View ArticlePubMedPubMed CentralGoogle Scholar
- Popovich K, Hota B, Rice T, Aroutcheva A, Weinstein RA. Phenotypic prediction rule for community-associated methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2007;45:2293–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Otter JA, French GL. Utility of antimicrobial susceptibility-based algorithms for the presumptive identification of genotypically-defined community-associated methicillin-resistant Staphylococcus aureus at a London teaching hospital. Eur J Clin Microbiol Infect Dis. 2011;30:459–63.View ArticlePubMedGoogle Scholar
- Gbaguidi-Haore H, Thouverez M, Couetdic G, Cholley P, Talon D, Bertrand X. Usefulness of antimicrobial resistance pattern for detecting PVL- or TSST-1-producing meticillin-resistant Staphylococcus aureus in a French university hospital. J Med Microbiol. 2009;58:1337–42.View ArticlePubMedGoogle Scholar
- Liu Q, Han L, Li B, Sun J, Ni Y. Virulence characteristic and MLST-agr genetic background of high-level mupirocin-resistant, MRSA isolates from Shanghai and Wenzhou. China PLoS One. 2012;7:e37005.View ArticlePubMedGoogle Scholar
- Yu F, Liu Y, Lv J, Qi X, Lu C, Ding Y, Li D, Liu H, Wang L. Antimicrobial susceptibility, virulence determinant carriage and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections. Braz J Infect Dis. 2015;19:614–22.View ArticlePubMedGoogle Scholar
- Yu F, Li T, Huang X, Xie J, Xu Y, Tu J, Qin Z, Parsons C, Wang J, Hu L, Wang L. Virulence gene profiling and molecular characterization of hospital-acquired Staphylococcus aureus isolates associated with bloodstream infection. Diagn Microbiol Infect Dis. 2012;74:363–8.View ArticlePubMedGoogle Scholar
- Wu D, Li X, Yang Y, Zheng Y, Wang C, Deng L, Liu L, Li C, Shang Y, Zhao C, et al. Superantigen gene profiles and presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus isolated from Chinese children. J Med Microbiol. 2011;60:35–45.View ArticlePubMedGoogle Scholar
- He W, Chen H, Zhao C, Zhang F, Li H, Wang Q, Wang X, Wang H. Population structure and characterisation of Staphylococcus aureus from bacteraemia at multiple hospitals in China: association between antimicrobial resistance, toxin genes and genotypes. Int J Antimicrob Agents. 2013;42:211–9.View ArticlePubMedGoogle Scholar
- Diep BA, Carleton HA, Chang RF, Sensabaugh GF, Perdreau-Remington F. Roles of 34 virulence genes in the evolution of hospital- and community-associated strains of methicillin-resistant Staphylococcus aureus. J Infect Dis. 2006;193:1495–503.View ArticlePubMedGoogle Scholar
- Tristan A, Ferry T, Durand G, Dauwalder O, Bes M, Lina G, Vandenesch F, Etienne J. Virulence determinants in community and hospital meticillin-resistant Staphylococcus aureus. J Hosp Infect. 2007;65 Suppl 2:105–9.View ArticlePubMedGoogle Scholar
- Maree CL, Daum RS, Boyle-Vavra S, Matayoshi K, Miller LG. Community-associated methicillin-resistant Staphylococcus aureus isolates causing healthcare-associated infections. Emerg Infect Dis. 2007;13:236–42.View ArticlePubMedPubMed CentralGoogle Scholar
- Li DZ, Chen YS, Yang JP, Zhang W, Hu CP, Li JS, Mu L, Hu YH, Geng R, Hu K, et al. Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China. Chin Med J (Engl). 2011;124:687–92.View ArticleGoogle Scholar
- Zetola N, Francis JS, Nuermberger EL, Bishai WR. Community-acquired meticillin-resistant Staphylococcus aureus: an emerging threat. Lancet Infect Dis. 2005;5:275–86.View ArticlePubMedGoogle Scholar
- Collins J, Rudkin J, Recker M, Pozzi C, O’Gara JP, Massey RC. Offsetting virulence and antibiotic resistance costs by MRSA. Isme J. 2010;4:577–84.View ArticlePubMedGoogle Scholar