Reasons for the variation in reported treatment outcomes from antiretroviral therapy (ART) programmes in developing countries are not clearly defined.
Methods
Among ART-naïve individuals in a workplace ART programme in South Africa we determined virological outcomes at 12 months, and risk factors for suboptimal virological outcome, defined as plasma HIV-1 viral load >= 400 copies/ml.
Results
Among 1760 individuals starting ART before July 2004, 1172 were in follow-up at 12 months of whom 953 (81%) had a viral load measurement (median age 41 yrs, 96% male, median baseline CD4 count 156 × 106/l). 71% (681/953) had viral load < 400 copies/ml at 12 months. In a multivariable analysis, independent predictors of suboptimal virological outcome at 12 months were <1 log decrease in viral load at six weeks (odds ratio [OR] 4.71, 95% confidence interval [CI] 2.56–8.68), viral load at baseline (OR 3.63 [95% CI 1.88–7.00] and OR 3.54 [95% CI 1.79–7.00] for 10,001–100,000 and >100,000 compared to <= 10,000 copies/ml, respectively), adherence at six weeks (OR 3.50 [95% CI 1.92–6.35]), WHO stage (OR 2.08 [95% CI 1.28–3.34] and OR 2.03 [95% CI 1.14–3.62] for stage 3 and 4 compared to stage 1–2, respectively) and site of ART delivery. Site of delivery remained an independent risk factor even after adjustment for individual level factors. At 6 weeks, of 719 patients with self-reported adherence and viral load, 72 (10%) reported 100% adherence but had <1 log decrease in viral load; conversely, 60 (8%) reported <100% adherence but had >= 1 log decrease in viral load.
Conclusion
Virological response at six weeks after ART start was the strongest predictor of suboptimal virological outcome at 12 months, and may identify individuals who need interventions such as additional adherence support. Self reported adherence was less strongly associated but identified different patients compared with viral load at 6 weeks. Site of delivery had an important influence on virological outcomes; factors at the health system level which influence outcome need further investigation to guide development of effective ART programmes.
Background
An unprecedented effort by global organisations, governments and health care workers has achieved access to antiretroviral treatment (ART) for 2.0 million HIV-infected individuals in low- and middle-income countries by December 2006. Despite these efforts, 72% of the estimated 7.1 million who need ART globally are not on treatment, and hence strategies to improve access to care for HIV-infected persons have been the subject of much discussion [1, 2]. As more patients start ART, treatment programmes face the emerging challenge of achieving and maintaining virological suppression in increasing numbers of patients for sustained periods. Treatment success requires excellent adherence to ART, which is difficult to maintain [3]. A suboptimal virological response to ART may lead to increased transmission of ART-resistant virus strains and ultimately to a dramatic reduction of the therapeutic lifespan of available ART regimens [4–6].
ART programmes in developing countries have reported varied treatment outcomes. Pilot projects established by non-governmental organisations in South Africa and Haiti have reported good virological results [7–9] although others have been less encouraging [10–12]. Adherence to ART is a major determinant of virological outcome [3, 13, 14] and in industrialized countries other baseline factors influencing virological response have included disease stage [15], viral load [15, 16], and previous exposure to ART[15], although associations with baseline CD4 count and viral load are not consistent [17, 18]. Determinants of virological outcomes, and the effect of differences in the health delivery system on outcomes, have not been studied in detail in resource limited settings. Understanding these issues is important to guide the development of effective ART programmes.
Another obstacle to effective HIV care in resource-limited countries is limited access to laboratory facilities to monitor the virological and immunological markers that are considered the standard of care in wealthy nations. For middle-income settings, where there is limited laboratory support, data are needed to inform guidelines on how best to use scarce resources for laboratory monitoring; for example to decide at what point after starting ART it is most useful to measure viral load to ensure the best outcomes for patients.
We report risk factors for suboptimal virological outcome at 12 months among HIV-infected adults receiving ART in a workplace-based HIV care programme in South Africa.
Methods
ART clinic protocol
This workplace-based HIV care programme, primarily serving the mining workforce, has been described in detail elsewhere [19]. The programme was designed by Aurum Institute and has been implemented at workplace sites in Southern Africa, which vary from large hospital clinics through occupational health centres to general practitioners' offices. Standard guidelines were developed for the programme and are used at all sites; these provide guidance on all aspects of HIV care including provision of counseling, antiretroviral therapy guidelines, clinical and laboratory monitoring and opportunistic infection prophylaxis. ART is supplied either through a central pharmacy which supplies named-patient medication to sites via a courier, or through on-site pharmacies. Clinical data are collected on standardized forms and entered into a central database. Aurum staff provide training, based on the programme guidelines, for clinic staff both prior to programme initiation, and refresher training at intervals afterwards. Individual sites determine where within their health service the programme will be delivered, which pharmacy system is used, and manage clinic staff.
Eligibility for ART is determined using the CD4 count and World Health Organization (WHO) clinical stage, which, at the time of this study, was based on the 1990 definitions [20]: in brief, WHO stage 1 is asymptomatic infection, stage 2 is mild disease such as mucocutaneous manifestations, stage 3 is moderately severe disease but generally not defining AIDS (including pulmonary tuberculosis within the previous year) and stage 4 is severe disease including many AIDS-defining conditions. Individuals are eligible for ART if they have HIV infection and have a CD4 count <250 × 106/l regardless of World Health Organization (WHO) stage of HIV disease; or are in WHO clinical stage 3 with a CD4 count below 350 × 106/l; or are in WHO clinical stage 4, regardless of CD4 count. Prior to ART initiation, ART-eligible individuals have at least two clinic visits and participate in a preparatory three-step counselling process. Standardised drug regimens are used: the first line regimen is zidovudine, lamivudine and efavirenz, and the second line regimen is abacavir, didanosine and lopinavir-ritonavir. Single drug substitutions are made if necessary, for example, stavudine is substituted for zidovudine for patients with anaemia, and nevirapine for efavirenz for women of child-bearing potential. Other changes to the regimens can be made after consultation with the clinical HIV consultants. All ART starts, changes and stops are recorded by the clinician on a form which details the regimen and the reason for the decision.
At every clinic visit, data including self-reported adherence to ART, WHO clinical stage and weight are recorded. Individuals reporting less than perfect adherence are offered additional counseling. In accordance with clinic guidelines, CD4 count and plasma viral load are measured at baseline and at six weeks, six months and every six months thereafter.
A pharmacy register records when ART prescriptions are received and when prescriptions are collected from the pharmacy. If ART is not collected, clinic staff are notified, and the participant is invited back to the clinic for further evaluation and counselling.
Study design and participants
We analysed virological outcomes on ART using clinic data as an observational cohort. We included HIV-infected adults who were previously ART-naïve, who started ART before 1 July 2004 from nine companies (39 sites) who have at least 10 patients within their HIV care programme. We included follow-up data available up to 31 March 2006: thus all included individuals had the opportunity for at least 12 months of follow-up. The ART start date was defined as the date ART was first prescribed. For individuals who left the programme due to end of employment, death or other reasons, exit dates were determined using treatment stop forms completed by clinic physicians. Individuals who died were not considered as treatment failures, consistent with other reports [21] because most deaths in developing country ART programmes occur soon after ART start [22], before virological failure is likely to have occurred. Individuals who were lost to follow-up during the study were investigated to determine the date of and reason for discontinuation. The main analysis included all individuals with an HIV-1 RNA measurement at 12 months, and individuals who switched to second-line treatment prior to the 12 month visit, who were considered as having virological failure at 12 months. A further analysis also included individuals remaining in the workforce but who had stopped ART prior to the 12 month visit, with these individuals assumed to have virological failure at 12 months. Individuals who had discontinued because they left the workforce were excluded from all risk factor analysis. HIV-1 RNA levels at 6 weeks, 6 and 12 months were defined as the measurement taken between 28–120 days, 121–240 days and 300–450 days following initiation of ART, respectively. If there was more than one measurement in the interval the HIV-1 RNA taken closest to respective visit was used.
Laboratory protocol
Plasma HIV-1 RNA levels were determined at one central laboratory using the Versant HIV-1 RNA 3.0 assay (bDNA) (Bayer Corporation, Tarrytown, New York, USA.), measurement range 50–500,000 copies/ml. CD4 counts were determined using either FACSCOUNT (BD Biosciences, San Jose, California) or FACSCalibur (BD Biosciences, San Jose, California).
Data management and statistical analysis
Data were entered into a password protected Access database (Microsoft Office, 2000) and verified. Analysis was carried out using STATA v.9 (Stata Corporation, College Station, Texas, USA).
Plasma HIV-1 RNA concentrations were analysed using the log10 scale; values above or below the quantifiable range were assigned values at the respective limits of the assay. Risk factors for suboptimal virological outcome (>= 400 HIV-1 RNA copies/ml) at 12 months were analysed by logistic regression. Risk factors considered were primarily baseline (at start of ART) variables, but we also looked at virological response and self-reported adherence at six weeks, because this is the first point after ART start at which viral load is measured routinely and represents an early point at which interventions to promote adherence could be implemented if required. When analyzing the association with self-reported adherence to ART at the six-week visit, because only about 10% of individuals reported missing any ART, we categorized adherence as perfect (100% reported adherence in last 3 days) and non-perfect (<100%). Factors with a P value of < 0.10 were considered for the multivariable analysis. When considering programme delivery site as a risk factor, sites where greater than 100 individuals had started ART were considered as separate entities (sites A, B and C, each of which serves individuals working for a single company). Sites which were too small to be analyzed separately were grouped; those belonging to one company were considered as site D and all other sites as site E, on the basis that policy differed by company regarding implementation of the ART programme on issues such as pharmacy arrangements and clinic staffing levels. The likelihood ratio test was used to assess associations between risk factors and outcome.
Ethical considerations
This study was approved by the Research Ethics Committees of Anglogold Health Service, South Africa and the London School of Hygiene & Tropical Medicine, UK.
Results
A total of 1760 individuals had started ART before 1st July 2004, satisfied the other inclusion and exclusion criteria and were therefore eligible for the analysis. The median age was 41 years (range 21–66 years) and 97% (1701/1760) were male, reflecting the gender distribution of the workforce. Of the 1760 individuals, 175 (9.9%) and 213 (12.1%) had died or left the workforce before the 12 month follow-up, respectively, and 200 (11.4%) stopped ART before 12 months (Figure 1). Of the remaining 1172 individuals, 81.3% (953/1172) either had a viral load measurement at 12 months or started second line therapy prior to the 12 month visit, and data are subsequently reported for these individuals. Of those in the programme at 12 months, baseline CD4 counts, viral load and 6 week viral loads were similar in the 219 individuals who did not have viral load measured at 12 months compared with the 953 who did have viral load measured at 12 months. The median time from initiation of ART to the 6 week HIV-1 RNA measurement was 7.1 weeks (inter-quartile range [IQR]: 6.3–8.9). For the 6 and 12 month HIV-1 RNA measurements the median times from initiation of ART were 5.9 (IQR: 5.6–6.3) and 12.0 (IQR: 11.4–12.6) months, respectively.
Figure 1
Flow diagram showing cohort construction.
The age and gender distribution of individuals with a viral load result at 12 months was similar to the overall group (median age 41 years, 96% male). At the time of starting ART the median weight was 63 kg (IQR: 57–69 kg), median CD4 count was 156 × 106/l (IQR: 86–230 × 106/l) and 73.0% of individuals were in WHO stage 3 or 4 (Table 1). There were differences in baseline characteristics between sites, notably for WHO stage and CD4 count. At 6 weeks, 6 and 12 months the median (IQR) increases in CD4 count from baseline were 76 (21–142), 92 (28–162) and 107 (33–216) × 106/l, respectively. By 12 months the median gain in weight was 3 kg (IQR: -1 to 7 kg). At 6 weeks 88.6% (669/755) of individuals had a greater than 1 log decrease in viral load, and at 6 weeks, 6 and 12 months 81.2% (651/802), 78.2% (594/760) and 71.5% (681/953) of individuals had virological suppression (defined as viral load < 400 copies/ml), respectively. Of the 656 individuals who had viral load measurements at 6 weeks, 6 and 12 months 63.4% (n = 416) had suppressed viral load levels at all three time points.
Table 1
Baseline characteristics of the cohort overall and by ART delivery site
Overall
By ART delivery site
Site A
Site B
Site C
Site D
Site E
n
953
165
238
198
145
207
Age (years)
Median
41
38
39
44
39
44
IQR
35–46
34–41
34–44
39–48
32–46
37–49
Sex
Male (%)
95.9
97.0
97.1
99.0
91.0
94.2
CD4 (× 106/l)
Median
156
177
140
160
131
169
(IQR)
86–230
106–280
77–205
83–250
88–185
101–222
WHO stage*
1–2 (%)
27.0
16.0
12.8
28.4
38.8
45.7
3
46.2
36.4
47.7
53.6
45.5
45.7
4
26.8
47.5
39.6
18.0
15.7
8.7
Viral load (copies/ml)
<= 10,000 (%)
20.1
20.1
20.5
18.1
16.7
24.0
10,001–100,000
49.7
46.5
50.5
51.3
48.6
50.5
>100,000
30.3
33.3
29.0
30.6
34.8
25.5
Weight (kg)
Median
63
64
65
61
60
65
IQR
57–69
59–69
59–70
56–68
52–67
60–73
ART regimen
COM/EFV (%)
94.1
96.4
94.5
97.0
86.9
94.2
ART = antiretroviral therapy; IQR = interquartile range; WHO = World Health Organization; COM = Combivir; EFV = Efavirenz
* clinical staging of HIV disease (see methods for definition).
Table 2 summarises univariable risk factors for a suboptimal virological outcome at 12 months. Older age, higher viral load, lower weight and WHO stage 3 or 4 at baseline, less than a 1 log decrease in viral load at 6 weeks and less than 100% adherence at 6 weeks were all associated with an increased odds of suboptimal virological outcome at 12 months. Large differences in virological response at 12 months were also observed by site of delivery; the percentage with viral load >= 400 copies/ml at 12 months ranged from 18–39% across the five sites.
Table 2
Univariable and multivariable associations for suboptimal viral load response (>= 400 copies/ml) at 12 months
Total
Viral load
>= 400 copies/
ml N (%)
Unadjusted
Odds ratio
95%
confidence
interval
P value*
Adjusted
Odds ratio
95%
confidence
interval
P value*
Sex
Male
914
258 (28)
1
-
0.31
1
0.52
Female
39
14 (36)
1.42
0.73–2.78
1.38
0.53–3.61
Age – grouped
(years)
<35
225
53 (24)
1
0.16
1
0.33
35–39
199
51 (26)
1.12
0.72–1.74
1.50
0.83–2.71
40–44
227
70 (31)
1.45
0.95–2.20
Pt = 0.01
1.43
0.82–2.52
45–49
166
53 (32)
1.52
0.97–2.38
1.89
1.04–3.43
>= 50
136
45 (33)
1.60
1.00–2.57
1.50
0.77–2.93
Site
Site A
165
35 (21)
1
-
<0.001
1
<0.001
Site B
238
44 (18)
0.84
0.51–1.38
0.71
0.39–1.32
Site C
198
78 (39)
2.41
1.59–3.86
2.38
1.32–4.31
Site D
145
49 (34)
1.90
1.14–3.15
1.80
0.92–3.50
Site E
207
66 (32)
1.74
1.08–2.79
1.70
0.88–3.31
WHO stage**
(n = 55 missing)
1–2
242
52 (21)
1
-
0.02
1
0.007
3
415
131 (32)
1.69
1.16–2.44
2.08
1.28–3.34
4
241
66 (27)
1.38
0.91–2.09
2.03
1.14–3.62
CD4 count at baseline
(× 106/l) (n = 45 Missing)
<= 50
126
37 (29)
1
-
0.5
51–100
136
46 (34)
1.23
0.73–2.07
101–200
332
95 (29)
0.96
0.61–1.51
201–350
273
70 (26)
0.83
0.52–1.33
> 350
41
13 (32)
1.12
0.52–2.39
Viral load at baseline
(copies/ml) (n = 61 missing)
<= 10,000
179
36 (20)
1
0.02
1
<0.001
10,001–
443
134 (30)
1.72
1.13–2.62
3.63
1.88–7.00
100,000
270
85 (31)
1.83
1.17–2.85
3.54
1.79–7.00
>100,000
Weight (kg)
(n = 77 missing)
<= 50
54
22 (41)
1
-
0.02
51–60
285
90 (32)
0.67
0.37–1.22
61–70
349
83 (24)
0.45
0.25–0.82
Pt = 0.007
>70
185
46 (25)
0.48
0.25–0.91
ART regimen
COM/EFV
897
249 (28)
1
-
0.04
other
56
23 (41)
1.81
1.04–3.15
<1 log decrease in viral
load at six weeks
(n = 198 missing)
No
669
157 (23)
1
-
<0.001
1
<0.001
Yes
86
40 (47)
2.84
1.79–4.49
4.71
2.56–8.68
Self-reported adherence
at 6 weeks
(n = 73 missing)
100%
784
204 (26)
1
-
<0.001
1
<0.001
<100%
95
43 (45)
2.35
1.52–3.63
3.50
1.92–6.35
* P-value from likelihood ratio test; Pt = P-value for linear trend
** clinical staging of HIV disease (see methods for definition).
WHO = World Health Organization; ART = antiretroviral therapy; COM = Combivir; EFV = Efavirenz
The multivariable analysis is reported in Table 2. Virological response and self-reported adherence at six weeks were included in the same model as the data suggests these variables may capture different information (Table 3). Amongst n = 719 patients with both viral load and adherence measured at 6 weeks 18% had differing results; 10% (72/719) reported 100% adherence but had <1 log decrease in viral load, whereas 8% (60/719) reported <100% adherence but had satisfactory virological response. Site of ART delivery, WHO stage, viral load at baseline, unsatisfactory decrease in viral load at six weeks and less than 100% self-reported adherence at six weeks were all independent risk factors for suboptimal virological outcome.
A further analysis was carried out including the 200 individuals who had stopped ART prior to the 12 month visit. Reasons for stopping ART included poor patient adherence or adverse event (63%; n = 126), patient request (11%; n = 23), failure by patient to collect ART prescription (20%; n = 39) and 6% for other reasons. Assuming these additional 200 individuals had suboptimal virological outcome at 12 months 59.1% would have virological suppression at 12 months. Risk factors for poor outcome were similar to the analysis reported in Table 2.
Table 3
Cross tabulation of satisfactory virological response at 6 weeks and self-reported adherence at 6 weeks (n = 719)
Decrease in viral load at six weeks: n (% of overall total)
≥1 log
<1 log
Self-reported adherence at 6 weeks: n (% of overall total)
100%
578 (80.4)
72* (10.0)
<100%
60* (8.3)
9 (1.2)
* Discrepant results for virological response at 6 weeks and reported adherence at 6 weeks
Discussion
The earliest reports from ART programmes in low-income countries were from programmes managed by non-governmental organizations. In their early phases when ART availability was limited, some programmes had stringent entry criteria, requiring good adherence to visits and to preventive therapy before ART was started [7]. These entry criteria are likely to have resulted in a clinic population pre-selected for good adherence. An unintended additional consequence may have been very high mortality among individuals waiting to start treatment [23]. In our programme the criteria for offering ART were medical need, with no prerequisite to demonstrate adherence, making it more similar to routine programmes in industrialized countries. Virological outcomes in those who had a 12 month blood sample taken were similar to those reported from clinic cohorts in the United States [24], but losses from our programme are higher than reported from such cohorts, and this is a limitation of our study.
We can distinguish two main reasons for losses from our programme; individuals who have left the workforce, which may not relate to virological outcome, and those remaining in the workforce who have stopped ART, who may be assumed to have treatment failure. The reasons for default have been briefly reported elsewhere [25] and are currently being investigated in detail. From patient interview the reasons cited for leaving the programme included use of traditional medicines, side effects and not being convinced of the benefits of treatment. A systematic review of patient retention in ART programmes in sub-Saharan Africa found that the percentage of patients retained at 12 months from initiation of ART (where retention was defined as patients known to be alive and receiving ART) varied between 49%–90.3% (median 76%) [26]. Based on this definition patient retention in our study was comparable at 67%.
As ART roll out progresses, retention within programmes will become a key issue determining long-term success. Among patients in our programme who had a viral load measured, 71.5% had suppressed viral load at 12 months following initiation of ART. Using the same analysis approach other South African studies have reported better outcomes [7, 9] whilst a small study in Senegal reported 61.9% with viral load < 500 copies/ml at 12 months [10].
We found that high baseline viral load and more advanced WHO stage were risk factors for a suboptimal virological outcome at 12 months. A fall in viral load of less than one log at 6 weeks predicted a suboptimal virological outcome at 12 months, consistent with reports from industrialized countries [15, 16, 27, 28], as did reported less than perfect adherence at six weeks. In our setting there was very little ART use prior to the start of this cohort and hence transmitted resistance among our participants is unlikely; poor virological response is most likely to be due to poor adherence. Suboptimal adherence should be identified as early as possible, so that additional support can be provided, aiming to minimize the risk of ART resistance developing. In resource-limited settings, if it is possible to measure baseline viral load, an early measure of viral load on treatment (for example at four to six weeks) would be arguably more valuable than at the six month visit as suggested, for example, in South African guidelines [29]. Self-reported adherence was also a strong predictor of outcome at 12 months which may be useful if viral load measurements cannot be done.
Our programme provides an unusual opportunity to investigate the influence of health facility level, as well as individual level, factors on ART outcomes. Site was an important determinant of virological outcome which persisted after adjustment for individual factors. Given that all sites used the same clinical guidelines, criteria for starting ART, counseling protocols and drug regimens, it is interesting that site had such a large effect on the probability of achieving virological suppression. With relatively few large sites to compare we cannot formally investigate at the health facility level which may influence virological outcomes. However our experience suggests some factors which may be important include long waiting times for clients (both in clinics and in pharmacy); using staff who do not have specialized training in HIV care; failure to contact individuals who do not attend clinic and lack of communication between pharmacy and clinic staff if ART is not collected. We plan to explore these issues in further work, including comparison of costs at different clinic sites.
Conclusion
In conclusion, in this cohort of adults starting ART in South Africa, the strongest independent predictors of virological outcome at 12 months were the decrease in viral load and self-reported adherence at six weeks. In settings with limited access to viral load measurements, the virological response would be more usefully determined at this point rather than later, so that appropriate interventions can be implemented. Self-reported adherence at six weeks was also an independent predictor of outcome. Health system factors have an important influence on virological outcomes; this needs further investigation to guide implementation of effective ART programmes.
Declarations
Acknowledgements
We thank the many staff of Aurum Institute for Health Research, collaborators in the ART programme and the participating companies who contributed to the establishment and running of the HIV care programmes, and the many people who collected data for these studies.
Sources of support: This study was funded by Anglo American plc.
KLF was supported by the Aurum Institute for Health Research and a Bill and Melinda Gates Foundation grant for the Consortium to Respond Effectively to the AIDS-TB Epidemic (CREATE) Biostatistics core, SC, LFP, GJC by Aurum Institute for Health Research, ALS from the Trustees of LSHTM and Aurum Institute for Health Research, and ADG by a UK Department of Health Public Health Career scientist award.
Authors’ Affiliations
(1)
London School of Hygiene and Tropical Medicine
(2)
Aurum Institute for Health Research
(3)
Kimera Solutions
(4)
University of Pretoria
(5)
Desmond Tutu HIV Centre, Institute of Infectious Disease and Molecular Medicine, Faculty of Health, University of Cape Town
Paterson DL, Swindells S, Mohr J, Brester M, Vergis EN, Squier C, Wagener MM, Singh N: Adherence to protease inhibitor therapy and outcomes in patients with HIV infection. Ann Intern Med. 2000, 133: 21-30.View ArticlePubMedGoogle Scholar
Carpenter CC, Fischl MA, Hammer SM, Hirsch MS, Jacobsen DM, Katzenstein DA, Montaner JS, Richman DD, Saag MS, Schooley RT, Thompson MA, Vella S, Yeni PG, Volberding PA: Antiretroviral therapy for HIV infection in 1997. Updated recommendations of the International AIDS Society-USA panel. JAMA. 1997, 277: 1962-1969. 10.1001/jama.277.24.1962.View ArticlePubMedGoogle Scholar
García de Olalla P, Knobel H, Carmona A, Guelar A, López-Colomés JL, Caylà JA: Impact of adherence and highly active antiretroviral therapy on survival in HIV-infected patients. J Acquir Immune Deficiency. 2002, 30: 105-110. 10.1097/00126334-200205010-00014.View ArticleGoogle Scholar
Blower SM, Aschenbach AN, Gershengorn HB, Kahn JO: Predicting the unpredictable: Transmission of drug resistant HIV. Nat Med. 2001, 7: 1016-1020. 10.1038/nm0901-1016.View ArticlePubMedGoogle Scholar
Coetzee D, Hildebrand K, Boulle A, Maartens G, Louis F, Labatala V, Reuter H, Ntwana N, Goemaere E: Outcomes after two years of providing ART in Khayelitsha, South Africa. AIDS. 2004, 18: 887-895. 10.1097/00002030-200404090-00006.View ArticlePubMedGoogle Scholar
Severe P, Leger P, Charles M, Noel F, Bonhomme G, Bois G, George E, Kenel-Pierre S, Wright PF, Gulick R, Johnson WD, Pape JW, Fitzgerald DW: Antiretroviral therapy in a thousand patients with AIDS in Haiti. NEJM. 2005, 353: 2325-34. 10.1056/NEJMoa051908.View ArticlePubMedGoogle Scholar
Bekker LG, Myer L, Orrell C, Lawn S, Wood R: Rapid scale-up of community-based HIV treatment service. Programme performance over 3 consecutive years in Guguletu, South Africa. S Afr Med J. 2006, 96 (4): 315-320.PubMedGoogle Scholar
Laurent C, Diakhaté N, Gueye NF, Touré MA, Sow PS, Faye MA, Gueye M, Lanièce I, Touré Kane C, Liégeois F, Vergne L, Mboup S, Badiane S, Ndoye I, Delaporte E: The Senegalese government's highly active antiretroviral therapy initiative: an 18-month follow-up study. AIDS. 2002, 16: 1363-70. 10.1097/00002030-200207050-00008.View ArticlePubMedGoogle Scholar
Macharia DK, Chang LW, Lule G, Owili DM, Tesfaledet G, Patel S, Silverstein DM, Ng'ang'a L, Bush T, De Cock KM, Weidle PJ: Antiretroviral therapy in the private sector of Nairobi, Kenya: a review of the experience of five physicians. AIDS. 2003, 17: 938-940. 10.1097/00002030-200304110-00028.View ArticlePubMedGoogle Scholar
Djomand G, Roels T, Ellerbrock T, Hanson D, Diomande F, Monga B, Maurice C, Nkengasong J, Konan-Koko R, Kadio A, Wiktor S, Lackritz E, Saba J, Chorba T: Virological and immunological outcomes and programmatic challenges of an antiretroviral treatment pilot project in Abidjan, Côte d'Ivoire. AIDS. 2003, 17 (suppl 3): S5-15.View ArticlePubMedGoogle Scholar
Hofer CB, Schechter M, Harrison LH: Effectiveness of antiretroviral therapy among patients who attend public HIV clinics in Rio de Janeiro, Brazil. J Acquir Immune Defic Syndr. 2004, 36: 967-71. 10.1097/00126334-200408010-00011.View ArticlePubMedGoogle Scholar
Knobel H, Guelar A, Carmona A, Espona M, González A, López-Colomés JL, Saballs P, Gimeno JL, Díez A: Virological outcomes and predictors of virological failure of active antiretroviral therapy containing protease inhibitors. AIDS Patient Care STDs. 2001, 15: 193-9. 10.1089/10872910151133729.View ArticlePubMedGoogle Scholar
Kitchen CM, Kitchen SG, Dubin JA, Gottlieb MS: Initial virological and immunological response to highly active antiretroviral therapy predicts long-term clinical outcome. Clin Infect Dis. 2001, 33: 466-472. 10.1086/321900.View ArticlePubMedGoogle Scholar
Phillips AN, Staszewski S, Weber R, Kirk O, Francioli P, Miller V, Vernazza P, Lundgren JD, Ledergerber B, Swiss HIV Cohort Study; Frakfurt HIV Clinic Cohort; EuroSIDA Study Group: HIV viral load response to antiretroviral therapy according to the baseline CD4 cell count and viral load. JAMA. 2001, 286: 2560-7. 10.1001/jama.286.20.2560.View ArticlePubMedGoogle Scholar
Bartlett JA, DeMasi R, Quinn J, Moxham C, Rousseau F: Overview of the effectiveness of triple combination therapy in antiretroviral-naïve HIV-1 infected adults. AIDS. 2001, 15: 1369-1377. 10.1097/00002030-200107270-00006.View ArticlePubMedGoogle Scholar
Charalambous S, Grant AD, Day JH, Pemba L, Chaisson RE, Kruger P, Martin D, Wood R, Brink B, Churchyard GJ: Establishing a workplace antiretroviral therapy programme in South Africa. AIDS Care. 2007, 19: 34-41. 10.1080/09500340600677872.View ArticlePubMedGoogle Scholar
WHO: Interim proposal for a WHO staging system for HIV infection and disease. Weekly Epidemiological Record. 1990, 65: 221-224.Google Scholar
Coetzee D, Boulle A, Hildebrand K, Asselman V, Van Cutsem G, Goemaere E: Promoting adherence to antiretroviral therapy: the experience from a primary care setting in Khayelitsha, South Africa. AIDS. 2004, 18 (suppl 3): S27-S31. 10.1097/00002030-200406003-00006.View ArticlePubMedGoogle Scholar
The Antiretroviral Therapy in Lower Income Countries (ART-LINC) and ART Cohort Collaboration (ART-CC) groups: Mortality of HIV-1 infected patients in the first year of antiretroviral therapy: comparison between low-income and high-income countries. Lancet. 2006, 367: 817-824. 10.1016/S0140-6736(06)68337-2.View ArticleGoogle Scholar
Lawn SD, Myer L, Orrell C, Bekker LG, Wood R: Early mortality among adults accessing a community-based antiretroviral service in South Africa: implications for programme design. AIDS. 2005, 19: 2141-2148.View ArticlePubMedGoogle Scholar
Moore RD, Keruly JC, Gebo KA, Lucas GM: An improvement in virologic response to highly active antiretroviral therapy in clinical practice from 1996 through 2002. J Acquir Immune Defic Syndr. 2005, 39: 195-198.PubMedGoogle Scholar
Charalambous S, Innes C, Muirhead D, Kumaranayake L, Fielding K, Pemba L, Hamilton R, Grant A, Churchyard GJ: Evaluation of a workplace HIV treatment programme in South Africa. AIDS. 2007, 21 (suppl 3): S73-S78. 10.1097/01.aids.0000279696.63438.aa.View ArticlePubMedGoogle Scholar
Rosen S, Fox MP, Gill CJ: Patient retention in antiretroviral therapy programs in sub-Saharan Africa: a systematic review. PLoS Med. 2007, 4 (10): e298-10.1371/journal.pmed.0040298.View ArticlePubMedPubMed CentralGoogle Scholar
Lepri AC, Miller V, Phillips AN, Rabenau H, Sabin CA, Staszewski S: The virological response to highly active antiretroviral therapy over the first 24 weeks of therapy according to the pre-therapy viral load and the weeks 4–8 viral load. AIDS. 2001, 15: 47-54. 10.1097/00002030-200101050-00008.View ArticlePubMedGoogle Scholar
Smith CJ, Staszewski S, Sabin CA, Nelson M, Dauer B, Gute P, Johnson MA, Phillips AN, Gazzard B: Use of viral load measured after 4 weeks of highly active antiretroviral therapy to predict virologic outcome at 24 weeks for HIV-1-positive individuals. J Acquir Immune Defic Syndr. 2004, 37: 1155-1159. 10.1097/01.qai.0000135958.80919.e4.View ArticlePubMedGoogle Scholar
Department of Health, South Africa. Training manual – Operational plan for comprehensive HIV and AIDS care, management and treatment for South Africa. 2nd edition, January 2005. [http://www.doh.gov.za/docs/hivaids-progressrep.html]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
