- Research article
- Open Access
- Open Peer Review
Increasing the frequency of hand washing by healthcare workers does not lead to commensurate reductions in staphylococcal infection in a hospital ward
© Beggs et al; licensee BioMed Central Ltd. 2008
- Received: 20 March 2008
- Accepted: 02 September 2008
- Published: 02 September 2008
Hand hygiene is generally considered to be the most important measure that can be applied to prevent the spread of healthcare-associated infection (HAI). Continuous emphasis on this intervention has lead to the widespread opinion that HAI rates can be greatly reduced by increased hand hygiene compliance alone. However, this assumes that the effectiveness of hand hygiene is not constrained by other factors and that improved compliance in excess of a given level, in itself, will result in a commensurate reduction in the incidence of HAI. However, several researchers have found the law of diminishing returns to apply to hand hygiene, with the greatest benefits occurring in the first 20% or so of compliance, and others have demonstrated that poor cohorting of nursing staff profoundly influences the effectiveness of hand hygiene measures. Collectively, these findings raise intriguing questions about the extent to which increasing compliance alone can further reduce rates of HAI.
In order to investigate these issues further, we constructed a deterministic Ross-Macdonald model and applied it to a hypothetical general medical ward. In this model the transmission of staphylococcal infection was assumed to occur after contact with the transiently colonized hands of HCWs, who, in turn, acquire contamination only by touching colonized patients. The aim of the study was to evaluate the impact of imperfect hand cleansing on the transmission of staphylococcal infection and to identify, whether there is a limit, above which further hand hygiene compliance is unlikely to be of benefit.
The model demonstrated that if transmission is solely via the hands of HCWs, it should, under most circumstances, be possible to prevent outbreaks of staphylococcal infection from occurring at a hand cleansing frequencies < 50%, even with imperfect hand hygiene. The analysis also indicated that the relationship between hand cleansing efficacy and frequency is not linear – as efficacy decreases, so the hand cleansing frequency required to ensure R0 < 1 increases disproportionately.
Although our study confirmed hand hygiene to be an effective control measure, it demonstrated that the law of diminishing returns applies, with the greatest benefit derived from the first 20% or so of compliance. Indeed, our analysis suggests that there is little benefit to be accrued from very high levels of hand cleansing and that in most situations compliance > 40% should be enough to prevent outbreaks of staphylococcal infection occurring, if transmission is solely via the hands of HCWs. Furthermore we identified a non-linear relationship between hand cleansing efficacy and frequency, suggesting that it is important to maximise the efficacy of the hand cleansing process.
- Hand Hygiene
- Staphylococcal Infection
- Hand Hygiene Compliance
- Puerperal Fever
- Basic Reproductive Number
Hand hygiene is generally considered to be the most important measure that can be applied to prevent the spread of healthcare-associated infection (HAI) . Through regular cleansing of hands, healthcare workers (HCWs) reduce the risk to transmitting nosocomial pathogens between patients and thus reduce the risk of exogenously-acquired infection. This has lead to the widespread opinion that HAI rates can be greatly reduced by increased hand hygiene compliance alone , with the result that healthcare authorities around the world have vigorously promoted hand hygiene as the pre-eminent measure in controlling HAI. However, this approach assumes that the effectiveness of hand hygiene is not limited by other factors and that greater compliance will continue to yield improved results. While it is undoubtedly the case that improved hand hygiene is beneficial [1, 3], there is growing evidence that increased compliance may not yield the hoped for results. For example, using dynamic transmission models, Cooper et al  and McBryde et al  found that the law of diminishing returns applies to hand hygiene, with the greatest benefits occurring in the first 20% or so of compliance. Furthermore, Austin et al  and Beggs et al  demonstrated that poor cohorting of nursing staff profoundly influences the effectiveness of hand hygiene measures. Moreover, Grundmann et al  found that during periods of under-staffing, it is necessary to greatly increase the frequency of hand cleansing in order to prevent outbreaks of infection. Collectively, these findings raise intriguing questions about the limitations of hand hygiene and the extent to which increasing compliance alone can further reduce the spread of infection. In order to investigate this issue further, we constructed a deterministic Ross-Macdonald model to analyse the hypothetical general medical ward presented by Cooper et al  who found that hand hygiene compliance levels > 30% made little impact on the prevalence of Staphylococcus aureus infection on the ward. However, in their study they assumed that each hand cleansing event had an efficacy of 100%, – which in "real life" situations on a busy hospital ward is unlikely to be the case. Given this, we decided to repeat the study of Cooper et al by using a model that takes into account not only the frequency of handwashing but also the efficacy of the hand cleansing process. The aim of the study was to evaluate the impact of imperfect hand cleansing on the transmission of staphylococcal infection and to identify whether there is a limit, above which further compliance would be unlikely to yield beneficial results.
Since Ignaz Semmelweis reported in 1847 that the incidence of puerperal fever in an obstetric unit could be drastically reduced through handwashing , cleansing of hands has been the principal measure employed in hospitals to reduce HAI. Until recently, hand cleansing was achieved through the use of soap and water, a time consuming  and sometimes inefficient process. However, in recent years, new alcohol-based products have become widely available. These are more convenient and quicker to use than soap and water and their use is being vigorously promoted. Despite this, levels of hand hygiene compliance remain low; typically < 50% [11–13]. One reason for low compliance appears to be the large number of handwashing opportunities that arise during patient care. These make it difficult for HCWs to cleanse their hands effectively, while still carrying out their clinical duties. For example, Pittet et al  observed an average of 43.4 hand hygiene opportunities per hour of patient care on an intensive care unit (ICU), which suggests that busy staff have very little spare time in which to cleanse their hands.
Although there are relatively few data on the types of patient-care activities that result in transmission of pathogens on the hands of HCWs, there is clear evidence that such transmission does occur. In a study of 'clean' activities, such as lifting patients, palpation of pulses or during sphygmomanometry, Casewell and Phillips  found that nurses could contaminate their hands with 100–1000 colony forming units (cfu) of Klebsiella spp. Similarly, Ehrenkranz and Alfonso  found that nurses readily contaminated their hands (i.e. 10–600 cfu/mL in glove juice samples) by touching the groins of patients heavily colonised with Proteus mirabilis. This suggests that contamination of the hands of HCWs is a frequent occurrence, and that relatively innocuous procedures can result in transient colonization. This is supported by the results of a study undertaken before the use of gloves by HCWs became common practice, which reported that 15% of nurses working in an isolation unit carried a median of 104 cfu of S. aureus on their hands and 29% of nurses working in a general hospital had a median count of 3.8 × 103 cfu . In another study, Daschner  found that S. aureus could be recovered from the hands of 21% of HCWs on an ICU, and that 21% of doctors and 5% of nurse carriers had > 1,000 cfu of the organism on their hands. Collectively, these data reveal that the hands of HCWs can become heavily contaminated when undertaking clinical procedures and they reinforce the need to maintain good hand hygiene procedures.
Hand cleansing is an imperfect process, the efficacy of which depends on the product used, the technique employed, and the duration of the process. Thus it is likely that it will not remove all the microorganisms from the hands of HCWs. Girou et al , for example, found that hand rubbing with a 75% alcohol-based solution resulted in a median percentage reduction in bacterial contamination of 83% compared with a reduction of only 58% when washing with medicated soap. Similarly, Zaragoza et al  found that the use of an alcoholic solution resulted in an average reduction in cfu of 88.2% compared with only 49.6% when soap and water was used.
In order to investigate the impact of sub-optimal hand cleansing on the transmission of staphylococcal infection, we modified the model of Cooper et al , so that it considered both handwashing frequency and handwashing efficacy (see Appendix) allowing us to simulate the effect of imperfect hand cleansing, something which other investigators had overlooked in their respective studies [3–6, 20].
Parameters and their default values
Number of patients
Number of health care workers (HCWs)
Patient removal rate
0.10 per day
14.0 per day
Average efficacy of each handwashing event
0.5 (i.e. 50%)
Detection rate of colonized patients
0.10 per day
Proportion of admissions already colonized
Patient-HCW contact rate
5 per patient per HCW per day
HCW-patient transmission probability (i.e. transmissibility)
Patient-HCW transmission probability (i.e. transmissibility)
HCW-patient transmission rate (β = cp)
Patient-HCW transmission rate (β' = cp')
The basic reproductive number, R 0, is the average number of secondary cases of colonization (which precedes infection) generated by one primary case in the absence of any infection control procedures.
Highly transmissible infections exhibit a large R 0, whereas those which are less transmissible have a smaller value of R 0. If the value of R 0 is greater than 1, then each colonized patient will generate further new cases and it is likely that an outbreak will ensue. The outbreak will continue until R 0 becomes less than 1, at which point it should begin to die out.
In the model it was assumed that:
• The transmission of staphylococci is caused by contact with the transiently colonized hands of HCWs. Contacts between transiently colonized HCWs and uncolonized patients have a given probability of colonizing the patient, which is termed the HCW-to-patient transmissibility. In the model we did not consider the possibility of direct patient-to-patient contacts.
• HCWs acquire transient hand-contamination only by touching colonized patients. All such contacts between uncolonized HCWs and colonized patients have a given probability of colonizing the carer, which is termed the patient-to-HCW transmissibility. In the model we did not take into account direct HCW-to-HCW transmission or the possibility of HCWs becoming colonized from external sources.
• The HCW-to-patient transmissibility is assumed to be equal to the patient-to-HCW transmissibility.
• The population of patients is assumed to be homogeneous, with each patient considered equally likely to be in contact with a HCW in any time interval, equally likely to become colonized, and when colonized, equally likely to transmit the pathogen to a HCW on contact.
• The population of HCWs is assumed to be homogeneous. Variations between HCWs due to differences in behaviour (such as handwashing) and skin microflora are not considered.
• The detection of colonized patients is assumed to be a random process, with the mean detection time depending only on a constant level of surveillance activity.
• Once detected, colonized patients are assumed to be removed from the main ward and thus no longer a source of infection. Colonized patients who are not detected are removed from the ward at the same rate as uncolonized patients.
• Each time a HCW washes his or her hands only a portion of the transient microflora present is removed. The amount removed depends on the efficacy of the hand cleansing process.
• The efficacy of the hand cleansing process is assumed to be the same for all the HCWs on the ward.
Events and their rates
Rate of event
Patient removal (when no colonization detected)
μ(x + y)
Detection of colonized patient and removal
HCW hand cleaning
μ' (x' + y')
Removal of contamination from hands of HCWs
c(x + y)
Admission of uncolonized patient
(1 - σ) (μx + μy + γy)
Admission of colonized patient
σ(μx + μy + γy)
Where x represents the number of uncolonized patients, y represents the number of colonized patients, y' represents the number of HCWs with contaminated hands, and x' represents the number of HCWs whose hands are uncontaminated.
In our study we modelled the effect of varying the handwashing frequency on the transmission of staphylococcal infection within the ward. For each frequency we modelled three possible efficacy scenarios (i.e. that each handwashing event removed 58%, 83% and 100% respectively of contaminants from the hands of HCWs). Efficacies of 58% and 83% were selected because they represent the values found by Girou et al  for HCWs using antibacterial soap and an alcohol-based solution, respectively, in a clinical setting. Having simulated the impact of hand cleansing frequency on the prevalence of infection, we then modelled the impact on R 0 of changes in hand hygiene efficacy, contact transmissibility, and the HCW-patient contact rate.
While theoretical studies such as the one described in this paper can only ever be approximations of what happens in clinical practice, they can, nevertheless, yield important insights into the factors that influence the transmission of infection in hospitals. In particular, they can be useful when assessing the relative impact of various infection control measures. Provided that the data used, to a large extent, mirror the situation in a the clinical environment,, it is possible to identify general trends in the transmission dynamics. With respect to this, while the analysis presented above confirms the long held opinion that hand hygiene is an effective control measure; it also shows that the law of diminishing returns applies and that the greatest benefits are derived from the first 20% or so of compliance. Indeed, the shape of the prevalence curves presented in Figure 1 (which have the same form as those produced by Cooper et al ) suggests that little benefit is accrued from very high levels of hand cleansing. Above a certain threshold, which will vary depending on input data, the benefit of increased hand hygiene compliance appears to be minimal. In the case of the study reported here, the data show that, even under conditions of very high transmissibility, if an alcohol solution is used, it should be possible to ensure R 0 < 1 when compliance is in the region 55%. This appears to confirm the findings of other researchers. For example, Cooper et al  found that under conditions of relatively high transmissibility (i.e. p' = p = 0.13) it was possible to ensure R 0 < 1 with a hand cleansing frequency < 30%. McBryde et al , using a stochastic transmission model of an ICU, found that 48% hand hygiene compliance was required to ensure R 0 < 1. Investigating the transmission of vancomycin-resistant enterococci on an ICU, Austin et al  found that a hand cleansing frequency of 50.5% achieved an effective reproductive number, R e = 0.69, well below unity (R 0 = 1 when f h = 27.9% – extrapolated from the data of Austin). Although, these researchers assumed in their respective models a hand hygiene efficacy of 100%, their results are similar to ours, suggesting that, despite the fact that, in practise, hand cleansing is an imperfect process, it should be possible to prevent many staphylococcal outbreaks from occurring without the need to achieve excessively high hand hygiene compliance. Having said this, it is important to remember that all these researchers (including ourselves) assume the transmission of pathogens to occur exclusively via the hands of HCWs, which is unlikely to be the case . Pathogens can remain viable on inanimate surfaces for long periods of time  and if environmental contamination in any way contributes significantly to the transmission of staphylococcal infection, then the hand hygiene compliance levels stated above may not be adequate.
The results of our study indicate that the level of hand hygiene required to ensure R 0 < 1 is greatly influenced by the rate at which HCWs and patients make contact with each other, and the transmissibility of the contacts made – as these values increase, so the hand cleansing frequency required to prevent an outbreak also increases. Again, this confirms the findings of Cooper et al  who found transmissibility to be the single most influential variable in their study. In our study we assumed, as they did that, for each contact event, the probability of a HCW colonizing a patient is the same as the probability that the patient will contaminate the hands of a HCW. We did this to facilitate direct comparison with the work of Cooper et al . This however, may not be the case, since contamination of HCWs hands is relatively transient (lasting only for a few hours), whereas patients tend to remain colonized for the length of their stay in hospital. Therefore, each colonized patient will contaminate many HCWs (R p >> 1), whereas a contaminated HCW will colonize patients only infrequently (R h << 1) . Accordingly, Austin et al  used values of p' = 0.40 and p = 0.06, which equates to a combined (p' × p) value of 0.024. Similarly, Grundmann et al  using the same methodology, adopted values of p' = 0.152 and p = 0.01, equating to a combined (p' × p) value of 0.015. By comparison, we and Cooper et al  used a combined (p' × p) value of 0.010, which, although lower than that used by the other researchers, is still of the same order of magnitude. The default HCW-patient contact rate used in our model was that suggested by Cooper et al  (i.e. 5 contacts per patient per HCW per day); considerably greater than the value of 1.38 contacts per patient per HCW per day reported by Austin et al  and somewhat less than the value of 7.6 contacts per patient per HCW per day used by Grundmann et al . Collectively, this gives us confidence that our analysis is valid and that the variables used are realistic.
From the foregoing it can be seen that our results are consistent with earlier studies. It can therefore be concluded that it should be possible to prevent many outbreaks of staphylococcal infection through hand hygiene measures alone, even if high compliance rates are not achieved. In the study reported here it appears that compliance rates of 40% or so, should be adequate to prevent most outbreaks occurring. If this is indeed the case, then this raises questions as to why so many outbreaks of staphylococcal infection continue to occur, despite the fact that recorded hand hygiene compliance rates are generally in the region 40% [11–13]. While the reasons for this are unclear, there appear to be four possible explanations, details of which are as outlined below:
While recorded hand hygiene compliance is typically in the region 40% [11–13], it may be that the Hawthorne effect is at work and that observed hand hygiene does not reflect what actually happens in reality – the implication being that general hand washing rates might be considerably lower than 40%.
Another reason might be the way in which wards are organized and managed. For example, if a ward is overcrowded or under-staffed, then those nurses on duty will have to attend to more patients than usual and so the HCW-patient contact rate is likely to rise and with it the hand cleansing frequency required to ensure R 0 < 1. This was graphically illustrated by Grundmann et al , who, in their study on an ICU, found exposure to relative staff deficit to be the only factor significantly associated with MRSA transmission. Indeed, they predicted that it would require an additional 12% improvement in adherence to hand hygiene policies to compensate for staff shortages. Given that during this study, observed hand hygiene compliance was on average 59%, the investigators concluded that under conditions of overcrowding and high workload, it would be impossible for the nursing staff to achieve the required additional compliance. The HCW-patient contact rate is also influenced by the way in which nurses are organized. Beggs et al  demonstrated that if nursing staff are allowed to mix freely with patients, then the number of potential transmission routes will be high, leading to increased need for hand cleansing. In order to minimise the number of transmission routes it is necessary to cohort the nursing staff so that they cannot transfer pathogens between different groups of patients. Other studies have reached similar conclusions [5, 6, 12, 20, 22] – the higher the level of cohorting, the fewer the number of contacts between patients.
Another reason why improved hand hygiene compliance might not deliver the hoped-for results might be because environmental contamination may be important in the transmission of staphylococcal infection. A number of researchers have demonstrated that widespread environmental contamination can occur as a result of MRSA infection/colonization [22–26]. For example, Boyce et al.  in a study in a US hospital, found environmental contamination in 73% of the rooms of MRSA infected patients and 69% of colonized patients. Indeed, they found 27% of the surfaces sampled in rooms containing MRSA-infected patients to be contaminated with MRSA, with frequently contaminated objects including the floor, bed linen, patients' gowns, over-bed tables and sphygmomanometer cuffs. Others have cultured MRSA from the air in patient rooms [22, 24, 25], and Wilson et al  observed a strong correlation between the presence of MRSA patients and air samples yielding MRSA in an ICU. Although many accounts of environmental contamination have been published it has proved very difficult to determine causality, and it is not known to what extent environmental contamination contributes towards the transmission of staphylococcal infection. However, it is thought that such contamination may seed environmental reservoirs resulting in increased sporadic infection . If this is indeed the case, then it might explain why staphylococcal infection has been so difficult to eradicate using hand hygiene measures alone. Accordingly we would recommend that environmental contamination with the bacterium is considered in future models of S. aureus transmission within the hospital setting.
In this paper we used a deterministic model to analyse the transmission dynamics of staphylococcal infection. While this approach has validity, it is not without drawbacks. Deterministic dynamic models predict that the persistence of infection is only possible above a certain critical threshold, R o = 1 (i.e. one infected or colonized patient must transmit the pathogen to, on average, at least one other patient). However in reality, outbreaks can occur even when these threshold conditions appear not to be met (i.e. when R o < 1). Conversely, outbreaks may die out despite R o > 1. This is because stochastic effects often dominate in small populations, such as those found within hospitals . This means that while most outbreaks should be controlled when R o < 1, some will not. In a few cases, chance events will be such that outbreaks of staphylococcal infection may occur despite the presence of stringent control measures. While the use of stochastic modelling would have yielded data on the variance of the transmission dynamics, the focus of our paper is on the impact of hand hygiene on average prevalence curves, which can be predicted using deterministic methods – hence the strategy adopted in this paper.
In the model x represents the number of susceptible patients and y the number of colonized patients, with the total number of patients in the ward being a constant n (i.e. x + y = n). Similarly, the total number of carers is n', with x' representing those HCWs not carrying the pathogen and y' representing those who are temporarily colonized, where x' + y' = n'. The removal rate for uncolonized patients is μ, which includes removals due to death, transfers to other wards, and discharges. The detection rate of colonized patients is γ, which is assumed to be the rate at which colonized patients are deliberately removed from the ward. With regard to the cleansing of hands, the handwashing rate is μ' and the average efficacy of each handwashing event is λ'. The rate at which contamination is removed from the hands of HCWs on the ward is therefore μ' y'λ'.
In the model it is assumed that each patient requires a contact from a HCW in a given time interval with a given probability and that no superfluous contacts are made. The mean number of contacts required by each patient per day is defined as c, with p being the probability that a patient becomes colonized on contact with a contaminated HCW, and p' to be the probability that a HCW becomes contaminated on contact with a colonized patient. In the model we let β = cp and β' = cp'. The rates at which contacts occur that can potentially result in colonization are therefore βx (for patient colonization) and β' y (for HCW contamination). Since a fraction y'/n' of contacts will be with colonized HCWs, and x'/n' with susceptible HCWs, the rates for patient and carer colonization will be βxy'/n' and β'x'y/n' respectively. The proportion of patients admitted to the ward who are colonized or infected is σ, where(0 ≤ σ ≤ 1).
Based on the above, the model solves the following differential equations to determine the spread of infection/colonization on the ward.
The authors would like to thank Dr Gareth Nisbet for his assistance in obtaining data for the study.
The authors confirm that no funding was received for this study.
- Pittet D, Allegranzi B, Sax H, Dharan S, Pessoa-Silva CL, Donaldson L, Boyce JM: Evidence-based model for hand transmission during patient care and the role of improved practices. Lancet Infect Dis. 2006, 6 (10): 641-652. 10.1016/S1473-3099(06)70600-4.View ArticlePubMedGoogle Scholar
- Teare L, Cookson B, Stone S: Hand hygiene. Bmj. 2001, 323 (7310): 411-412. 10.1136/bmj.323.7310.411.View ArticlePubMedPubMed CentralGoogle Scholar
- Raboud J, Saskin R, Simor A, Loeb M, Green K, Low DE, McGeer A: Modeling transmission of methicillin-resistant Staphylococcus aureus among patients admitted to a hospital. Infect Control Hosp Epidemiol. 2005, 26 (7): 607-615. 10.1086/502589.View ArticlePubMedGoogle Scholar
- Cooper BS, Medley GF, Scott GM: Preliminary analysis of the transmission dynamics of nosocomial infections: stochastic and management effects. J Hosp Infect. 1999, 43 (2): 131-147. 10.1053/jhin.1998.0647.View ArticlePubMedGoogle Scholar
- McBryde ES, Pettitt AN, McElwain DL: A stochastic mathematical model of methicillin resistant Staphylococcus aureus transmission in an intensive care unit: predicting the impact of interventions. J Theor Biol. 2007, 245 (3): 470-481. 10.1016/j.jtbi.2006.11.008.View ArticlePubMedGoogle Scholar
- Austin DJ, Bonten MJ, Weinstein RA, Slaughter S, Anderson RM: Vancomycin-resistant enterococci in intensive-care hospital settings: transmission dynamics, persistence, and the impact of infection control programs. Proc Natl Acad Sci U S A. 1999, 96 (12): 6908-6913. 10.1073/pnas.96.12.6908.View ArticlePubMedPubMed CentralGoogle Scholar
- Beggs CB, Noakes CJ, Shepherd SJ, Kerr KG, Sleigh PA, Banfield K: The influence of nurse cohorting on hand hygiene effectiveness. Am J Infect Control. 2006, 34 (10): 621-626. 10.1016/j.ajic.2006.06.011.View ArticlePubMedGoogle Scholar
- Grundmann H, Hori S, Winter B, Tami A, Austin DJ: Risk factors for the transmission of methicillin-resistant Staphylococcus aureus in an adult intensive care unit: fitting a model to the data. J Infect Dis. 2002, 185 (4): 481-488. 10.1086/338568.View ArticlePubMedGoogle Scholar
- Semmelweis I: The etiology, concept and prophylaxis of childbed fever. 1861, Pest, Wien und Leipzig , C.A.Hartleben’s Verlag-ExpeditionGoogle Scholar
- Pittet D, Mourouga P, Perneger TV: Compliance with handwashing in a teaching hospital. Infection Control Program. Ann Intern Med. 1999, 130 (2): 126-130.View ArticlePubMedGoogle Scholar
- Pittet D: Improving adherence to hand hygiene practice: a multidisciplinary approach. Emerg Infect Dis. 2001, 7 (2): 234-240.View ArticlePubMedPubMed CentralGoogle Scholar
- McArdle FI, Lee RJ, Gibb AP, Walsh TS: How much time is needed for hand hygiene in intensive care? A prospective trained observer study of rates of contact between healthcare workers and intensive care patients. J Hosp Infect. 2006, 62 (3): 304-310. 10.1016/j.jhin.2005.09.019.View ArticlePubMedGoogle Scholar
- Larson E, Kretzer EK: Compliance with handwashing and barrier precautions. J Hosp Infect. 1995, 30 Suppl: 88-106. 10.1016/0195-6701(95)90010-1.View ArticlePubMedGoogle Scholar
- Casewell M, Phillips I: Hands as route of transmission for Klebsiella species. Br Med J. 1977, 2 (6098): 1315-1317.View ArticlePubMedPubMed CentralGoogle Scholar
- Ehrenkranz NJ, Alfonso BC: Failure of bland soap handwash to prevent hand transfer of patient bacteria to urethral catheters. Infect Control Hosp Epidemiol. 1991, 12 (11): 654-662.View ArticlePubMedGoogle Scholar
- Ayliffe GA, Babb JR, Davies JG, Lilly HA: Hand disinfection: a comparison of various agents in laboratory and ward studies. J Hosp Infect. 1988, 11 (3): 226-243. 10.1016/0195-6701(88)90101-6.View ArticlePubMedGoogle Scholar
- Daschner FD: How cost-effective is the present use of antiseptics?. J Hosp Infect. 1988, 11 Suppl A: 227-235. 10.1016/0195-6701(88)90192-2.View ArticlePubMedGoogle Scholar
- Girou E, Loyeau S, Legrand P, Oppein F, Brun-Buisson C: Efficacy of handrubbing with alcohol based solution versus standard handwashing with antiseptic soap: randomised clinical trial. Bmj. 2002, 325 (7360): 362-10.1136/bmj.325.7360.362.View ArticlePubMedPubMed CentralGoogle Scholar
- Zaragoza M, Salles M, Gomez J, Bayas JM, Trilla A: Handwashing with soap or alcoholic solutions? A randomized clinical trial of its effectiveness. Am J Infect Control. 1999, 27 (3): 258-261. 10.1053/ic.1999.v27.a97622.View ArticlePubMedGoogle Scholar
- Bonten MJ, Austin DJ, Lipsitch M: Understanding the spread of antibiotic resistant pathogens in hospitals: mathematical models as tools for control. Clin Infect Dis. 2001, 33 (10): 1739-1746. 10.1086/323761.View ArticlePubMedGoogle Scholar
- Kramer A, Schwebke I, Kampf G: How long do nosocomial pathogens persist on inanimate surfaces? A systematic review. BMC Infect Dis. 2006, 6: 130-10.1186/1471-2334-6-130.View ArticlePubMedPubMed CentralGoogle Scholar
- Rutala WA, Katz EB, Sherertz RJ, Sarubbi FA: Environmental study of a methicillin-resistant Staphylococcus aureus epidemic in a burn unit. J Clin Microbiol. 1983, 18 (3): 683-688.PubMedPubMed CentralGoogle Scholar
- Boyce JM, Potter-Bynoe G, Chenevert C, King T: Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications. Infect Control Hosp Epidemiol. 1997, 18 (9): 622-627.View ArticlePubMedGoogle Scholar
- Shiomori T, Miyamoto H, Makishima K: Significance of airborne transmission of methicillin-resistant Staphylococcus aureus in an otolaryngology-head and neck surgery unit. Arch Otolaryngol Head Neck Surg. 2001, 127 (6): 644-648.View ArticlePubMedGoogle Scholar
- Shiomori T, Miyamoto H, Makishima K, Yoshida M, Fujiyoshi T, Udaka T, Inaba T, Hiraki N: Evaluation of bedmaking-related airborne and surface methicillin-resistant Staphylococcus aureus contamination. J Hosp Infect. 2002, 50 (1): 30-35. 10.1053/jhin.2001.1136.View ArticlePubMedGoogle Scholar
- Wilson RD, Huang SJ, McLean AS: The correlation between airborne methicillin-resistant Staphylococcus aureus with the presence of MRSA colonized patients in a general intensive care unit. Anaesth Intensive Care. 2004, 32 (2): 202-209.PubMedGoogle Scholar
- Cooper BS, Stone SP, Kibbler CC, Cookson BD, Roberts JA, Medley GF, Duckworth GJ, Lai R, Ebrahim S: Systematic review of isolation policies in the hospital management of methicillin-resistant Staphylococcus aureus: a review of the literature with epidemiological and economic modelling. Health Technol Assess. 2003, 7 (39): 1-194.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/8/114/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.