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Response to antiretroviral therapy (ART): comparing women with previous use of zidovudine monotherapy (ZDVm) in pregnancy with ART naïve women
© Huntington et al.; licensee BioMed Central Ltd. 2014
Received: 5 November 2013
Accepted: 26 February 2014
Published: 4 March 2014
Short-term zidovudine monotherapy (ZDVm) remains an option for some pregnant HIV-positive women not requiring treatment for their own health but may affect treatment responses once antiretroviral therapy (ART) is subsequently started.
Data were obtained by linking two UK studies: the UK Collaborative HIV Cohort (UK CHIC) study and the National Study of HIV in Pregnancy and Childhood (NSHPC). Treatment responses were assessed for 2028 women initiating ART at least one year after HIV-diagnosis. Outcomes were compared using logistic regression, proportional hazards regression or linear regression.
In adjusted analyses, ART-naïve (n = 1937) and ZDVm-experienced (n = 91) women had similar increases in CD4 count and a similar proportion achieving virological suppression; both groups had a low risk of AIDS.
In this setting, antenatal ZDVm exposure did not adversely impact on outcomes once ART was initiated for the woman’s health.
In the UK, zidovudine monotherapy (ZDVm) has been widely used for prevention of mother-to-child-transmission (PMTCT). Although combination antiretroviral therapy (ART) is now more commonly used for this purpose, ZDVm remains an option for pregnant women not on therapeutic ART with high CD4 counts (>350 cells/mm3) , low viral loads (<10,000 copies/ml), and who are willing to deliver by elective caesarean section . The 2012 BHIVA guidelines recommend that women opting to use ZDVm for PMTCT start ZDVm before 24 weeks of pregnancy . Pregnant women not on ART with CD4 ≤ 350 cells/mm3 are recommended to initiate long-term ART, as per the general UK HIV treatment guidelines .
Little is known about the impact of short-term ZDVm exposure on the woman’s subsequent response to ART when started for her own health. In low- and middle-income settings use of single-dose nevirapine (sd-NVP) can have a negative impact on subsequent treatment responses to NVP-containing regimens, with high levels of drug resistance, particularly when ART is initiated within 6–12 months post-sd-NVP exposure [4, 5]. However, whereas resistance to NVP requires a single mutation, resistance to ZDV requires multiple sequential mutations. As such, the development of resistance following short-term ZDVm for PMTCT is uncommon [6–9] and limited to women with more advanced disease [10–13] who would not meet clinical criteria for ZDVm PMTCT use . In contrast to the UK guidelines, ZDVm for PMTCT is no longer recommended within the updated consolidated World Health Organization (WHO) guidelines on use of ART; in line with the WHO guidelines’ main focus on earlier initiation of ART to decrease transmission, pregnant women not yet on therapy are recommended to start long-term combination therapy regardless of CD4 count . Despite these recent changes, there are many women in low- and middle-income settings with previous antenatal exposure to ZDVm who have yet to start ART for their own health . In addition, use of ZDVm in pregnancy will remain a strategy for PMTCT in some settings until combination ART becomes more accessible.
Our aim was to test whether short-term exposure to ZDVm in a previous pregnancy has an adverse effect on treatment outcomes once a woman starts ART for her own health. Record linkage between the UK and Ireland National Study of HIV in Pregnancy and Childhood (NSHPC) and the UK Collaborative HIV Cohort (UK CHIC) study gave us the opportunity to address this issue, which has implications for women with previous ZDVm experience.
We compared treatment outcomes among ART-naïve and ZDVm-experienced women starting therapeutic ART for their own health. Data were obtained from the UK CHIC study, an observational cohort that collates clinical data for adults receiving HIV-care at 15 large HIV clinics , and the NSHPC which collects antenatal data on all pregnant women diagnosed HIV-positive in the UK and Ireland . Women reported to both studies were linked using demographic and clinical variables, as described elsewhere . Data were not available on whether women had pregnancies prior to HIV diagnosis or infection, nor were data available on previous ART use outside the UK. Eligibility criteria were: initiating therapeutic ART at a UK CHIC site in 2000–2009 at least one year after HIV-diagnosis, either ZDVm-experienced or ART-naïve (combination ART-experienced women were excluded), and aged ≤49 years at HIV-diagnosis. Women were categorised as ZDVm-experienced if, according to NSHPC or UK CHIC data, they had ever used short-term ZDVm during pregnancy.
Baseline CD4 count and viral load were taken as the latest measurement within the three months before ART initiation. Characteristics of women starting treatment were compared using the Chi-square, Fisher’s exact or Wilcoxon two-sample test. ART outcomes were compared using logistic regression, proportional hazards regression or linear regression.
The UK CHIC Study has multicentre ethics committee approval (MREC/00/7/47). Ethics approval for NSHPC was renewed following review by the London Multi-Centre Research Ethics Committee in 2004 (MREC/04/2/009).
Overall, 1937 ART-naïve and 91 ZDVm-experienced women started therapeutic ART in 2000–2009. ZDVm-experienced women had used ZDVm in either one (n = 84) or two pregnancies (n = 7). No infants acquired HIV. ZDVm was used for a median of 12 weeks (IQR 8–16) and was typically started at 28 weeks gestation (range 17–39, IQR 24–31). The median duration between delivery (of the latest pregnancy) and starting therapy was 43 months (IQR 30–63); six women started within 12 months of delivery, none within 6 months.
In both groups some women were known to have had one or more previous pregnancies during which no ART was used (2.6% (51/1937) ART-naïve women and 11.0% (10/91) ZDV-experienced women). These pregnancies either ended early, due to termination or miscarriage, or resulted in a live birth where HIV was not diagnosed until delivery.
Characteristics of ART-naïve and ZDVm-experienced women when starting therapeutic ART in 2000-2009
Demographic and clinical characteristics at start of therapeutic ART
ZDVm-experienced (n = 91)
ART-naïve (n = 1937)
Age (years), median (IQR)
Time since HIV diagnosis (years), median (IQR)
Pregnant, n (%)
Ethnicity, n (%)
Risk group, n (%)
Year, n (%)
ART regimen started, n (%)
PI based (boosted and non-boosted)
Baseline CD4 count
Median (IQR), (cells/mm3)
(n = 75, n = 1431)
CD4 <200 cells/mm3, n (%)
CD4 <350 cells/mm3, n (%)
Baseline viral load
Median (IQR), (log10 copies/ml)
(n = 68, n = 1371)
≤50 copies/ml, n (%)
≤400 copies/ml, n (%)
≤10,000 copies/ml, n (%)
Hepatitis C co-infection, n (%)
Hepatitis B co-infection, n (%)
Previous AIDS event, n (%)
ZDVm-experienced and ART-naïve women started therapeutic ART at similar baseline CD4 counts (ZDVm-experienced: 226 [162–339] cells/mm3; ART-naïve: 225 [150–302] cells/mm3, p = 0.16) and viral load (ZDVm-experienced: 4.1 [3.2-4.5] log10 copies/ml; ART-naïve: 4.3 [3.4-4.9] log10 copies/ml, p = 0.08). Few women in either group were known to have hepatitis B (ZDVm-experienced: 1.1%; ART-naïve: 2.8%, p = 0.32) or hepatitis C co-infection (ZDVm-experienced: 4.4%; ART-naïve: 8.8%, p = 0.14). Few women had previously had an AIDS event (ZDVm-experienced: 7.7%; ART-naïve: 14.4%, p = 0.07).
Treatment outcomes for ART-naïve and ZDVm-experienced women starting therapeutic ART in 2000-2009
ZDVm-experienced N = 91
ART-naïve N = 1937
Death/AIDS event within 1 year, n (%)
CD4 cell count change at 6 months, median cells/mm3 (IQR)a
CD4 cell count change at 12 months, median cells/mm3 (IQR)b
Virological suppression at 6 months, n (%)c
Virological suppression at 12 months, n (%)d
Achieved virological suppression within 1 year, n (%)e
Virological rebound among those achieving virological suppression within 6 months, n (%)f
This UK study indicates that where ZDVm is used in pregnancy to prevent MTCT among women with high CD4 count and viral load <10,000 copies/ml it does not have a deleterious effect on treatment outcomes when ART is subsequently started. This adds support to the limited number of studies which indicate that short-term use of ZDVm for PMTCT is not detrimental to women’s long-term health [7, 9, 19, 20] and provides some reassurance with respect to the large number of women in lower-resourced settings with prior antenatal ZDVm exposure who have not yet initiated treatment. However, as a substantial proportion of these women may have had higher viral load in pregnancy [21, 22], their outcomes may be different. The increased likelihood of achieving viral suppression among ZDV-experienced women may be due to better treatment adherence or frequency of viral load monitoring. ZDVm-experienced women were more likely to have a viral load measure reported in the first year of treatment indicating that they had better contact with clinical care. If having a previous pregnancy, and short-term use of ART in that pregnancy, results in better engagement in clinical care when a woman subsequently starts therapy for her own health, this could mask any deleterious effect of the previous ART exposure. No data were available on previous pregnancies before HIV diagnosis or ART use outside the UK, something that may impact treatment outcomes. Therefore, further investigation is required to assess the long-term impact of short-term antenatal ART used for PMTCT.
In this setting, antenatal ZDVm exposure did not adversely impact on outcomes once ART was initiated for the woman’s health. This was a small study with limited statistical power and further research is required to support these findings.
UK CHIC: Steering Committee: Jonathan Ainsworth, Jane Anderson, Abdel Babiker, David Chadwick, Valerie Delpech, David Dunn, Martin Fisher, Sophie Jose, Brian Gazzard, Richard Gilson, Phillip Hay, Mark Gompels, Teresa Hill, Margaret Johnson, Stephen Kegg, Clifford Leen, Mark Nelson, Chloe Orkin, Adrian Palfreeman, Andrew Phillips, Deenan Pillay, Frank Post, Caroline Sabin (PI), Memory Sachikonye, Achim Schwenk, John Walsh.
Central Co-ordination: UCL Research Department of Infection & Population Health, Royal Free Campus, London (Teresa Hill, Sophie Jose, Susie Huntington, Andrew Phillips, Caroline Sabin, Alicia Thornton); Medical Research Council Clinical Trials Unit (MRC CTU), London (David Dunn, Adam Glabay).
Participating Centres: Barts and The London NHS Trust, London (Chloe Orkin, Nigel Garrett, Janet Lynch, James Hand, Carl de Souza); Brighton and Sussex University Hospitals NHS Trust (Martin Fisher, Nicky Perry, Stuart Tilbury, Elaney Youssef, Duncan Churchill); Chelsea and Westminster Hospital NHS Foundation Trust, London (Brian Gazzard, Mark Nelson, Matthew Waxman, David Asboe, Sundhiya Mandalia); Public Health England (PHE), London (Valerie Delpech); Homerton University Hospital NHS Trust, London (Jane Anderson, Sajid Munshi, Damilola Awosika); King’s College Hospital NHS Foundation Trust, London (Frank Post, Hardik Korat, Chris Taylor, Zachary Gleisner, Fowzia Ibrahim, Lucy Campbell); Medical Research Council Clinical Trials Unit (MRC CTU), London (Abdel Babiker, David Dunn, Adam Glabay); Middlesbrough, South Tees Hospitals NHS Foundation Trust, (David Chadwick, Kirsty Baillie); Mortimer Market Centre, University College London Medical School, London (Richard Gilson, Nataliya Brima, Ian Williams); North Middlesex University Hospital NHS Trust, London (Jonathan Ainsworth, Achim Schwenk, Sheila Miller, Chris Wood); Royal Free Hampstead NHS Trust/University College London Medical School, London (Margaret Johnson, Mike Youle, Fiona Lampe, Colette Smith, Helen Grabowska, Clinton Chaloner, Sophie Jose, Alicia Thornton, Teresa Hill, Susie Huntington, Andrew Phillips, Caroline Sabin); Imperial College Healthcare NHS Trust, London (John Walsh, Jonathan Weber, Farhan Ramzan, Mark Carder); The Lothian University Hospitals NHS Trust, Edinburgh (Clifford Leen, Alan Wilson); North Bristol NHS Trust (Mark Gompels, Sue Allan); Leicester, University Hospitals of Leicester NHS Trust (Adrian Palfreeman, Anne Moore, Lynn Fox, Josef Bojanowski); Woolwich, South London Healthcare NHS Trust (Stephen Kegg, Paul Main, Dr. Mitchell, Dr. Hunter), UK Community Advisory Board (Memory Sachikonye); St. George’s Healthcare NHS Trust (Phillip Hay, Mandip Dhillon).
NSHPC: We gratefully acknowledge the contribution of the midwives, obstetricians, genitourinary physicians, paediatricians, clinical nurse specialists and all other colleagues who report to the NSHPC through the British Paediatric Surveillance Unit of the Royal College of Paediatrics and Child Health, and the obstetric reporting scheme run under the auspices of the Royal College of Obstetricians and Gynaecologists. We thank Icina Shakes for administrative support. We also acknowledge the invaluable contribution of Janet Masters, NSHPC data manager and coordinator for many years until her death in 2012.
Sources of funding
The UK Collaborative HIV Cohort (UK CHIC) study is funded by the Medical Research Council (MRC), UK (grants G00001999 and G0600337). The National Study of HIV in Pregnancy and Childhood (NSHPC) receives core funding from PHE (previously the Health Protection Agency) (grant number GHP/003/013/003). Data are collated at the UCL Institute of Child Health which receives a proportion of funding from the Department of Health’s National Institute for Health Research Biomedical Research Centres funding Scheme. S Huntington has a UCL Studentship, funded by the MRC, for postgraduate work and C Thorne held a Wellcome Trust Research Career Development Fellowship. The views expressed in this manuscript are those of the researchers and not necessarily those of the funders.
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