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Decreased susceptibility of Neisseria gonorrhoeae isolates from Switzerland to Cefixime and Ceftriaxone: antimicrobial susceptibility data from 1990 and 2000 to 2012
© Kovari et al.; licensee BioMed Central Ltd. 2013
Received: 23 September 2013
Accepted: 18 December 2013
Published: 26 December 2013
Neisseria gonorrhoeae can rapidly develop resistance to antimicrobial agents. Over the last years, decreased gonococcal susceptibility to third-generation cephalosporins, especially cefixime, emerged worldwide. Therefore, current international guidelines recommend dual therapy for gonorrhoea with ceftriaxone plus either azithromycin or doxycycline. Gonococcal susceptibility data in Switzerland are sparse.
We investigated the prevalence of antibiotic susceptibility of N. gonorrhoeae in specimens collected between 1990 and 2012 at the University of Zurich, Switzerland. Minimum inhibitory concentrations (MICs) for cefixime, ceftriaxone, ciprofloxacin, and penicillin were determined by Etests. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints were used to define reduced susceptibility.
A total of 320 isolates were tested. Between 1990 and 2006 all tested samples were susceptible to both cephalosporins. Subsequently, the prevalence of elevated MICs for cefixime increased to 10.4% (2007/2008), 11.5% (2009/2010), and 11.4% (2011/2012); and for ceftriaxone to 2.4% (2007/2008), 4.7% (2009/2010), and 0% (2011/2012), respectively. The prevalence of resistance to ciprofloxacin (72.7%) and penicillin (22.7%) was high in 2011/2012.
Decreasing susceptibility of N. gonorrhoeae to third-generation cephalosporins in Switzerland supports treatment recommendations with ceftriaxone plus azithromycin or doxycycline. Health-care providers need to be aware of possible treatment failures with cephalosporins. Continued surveillance of gonococcal antimicrobial resistance is essential.
Infections with Neisseria gonorrhoeae may cause serious complications, including pelvic inflammatory disease, infertility, peritonitis, and chronic pain, and it enhances HIV transmission [1, 2]. Effective treatment of gonorrhoea is mandatory to control disease transmission and prevent sequelae. However, N. gonorrhoeae rapidly developed antimicrobial resistance to all previously recommended first-line drugs, including penicillins, tetracyclines, and fluoroquinolones. Third-generation cephalosporins, such as cefixime and ceftriaxone, remain the only readily available active antimicrobial class. In recent years, however, gonococcal strains with reduced susceptibility to cefixime emerged and spread worldwide . Clinical treatment failures with cefixime have been reported in Japan, several European countries, Canada, and South Africa [4–10]. In 2009, the first treatment failures to ceftriaxone were observed in Australia  and 2010 in Sweden . Currently, the identification of extensively drug-resistant (XDR) N. gonorrhoeae strains with high-level ceftriaxone resistance in Japan , France , and Spain , are of major concern. In addition to high ceftriaxone minimum inhibitory concentrations (MICs), these XDR strains are typically multidrug-resistant, exhibiting additional resistance to fluoroquinolones as well as to older drugs. Consequently, most treatment guidelines now recommend combination therapy with the injectable agent ceftriaxone plus either azithromycin or doxycycline instead of oral monotherapy with cefixime [16, 17].
In Switzerland, a strong rise in N. gonorrhoeae infections was observed during the past decade. Notifications have increased nearly threefold, with 521 reported cases in 2003, and 1569 cases in 2012, of which 77% occurred in men . Reporting gonorrhoea to public health authorities has been mandatory in Switzerland since 1988. However, gonococcal susceptibility data in Switzerland are sparse . Due to the current N. gonorrhoeae resistance problem, improving gonococcal antimicrobial resistance surveillance is of utmost importance and high on the agenda of the WHO’s “Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae, 2012” . Local antimicrobial surveillance is essential for evidence-based treatment recommendations. Given the lack of data, we investigated the distribution of antibiotic susceptibility of N. gonorrhoeae in specimens collected between 1990 and 2012.
All isolates were obtained in the northeastern part of Switzerland and the region of Zurich between January 1990 and December 2012. Specimens were cultured from symptomatic gonorrhoea patients with only one isolate taken per patient in case they were infected at multiple sites. Species confirmation and susceptibility testing was performed at the Institute of Medical Microbiology, University of Zurich. Specimens were cultured on selective media, i.e. until 2009 on Thayer Martin agar (Difco; Becton, Dickinson and Company, Basel, Switzerland), supplemented with IsoVitalex, and after 2009 on VCA3 agar (BioMérieux, Marcy-l’Etoile, France). The commercial biochemical gallery Api NH (BioMérieux) was used for the identification.
Minimum inhibitory concentrations (MICs) for cefixime, ceftriaxone, ciprofloxacin and penicillin were determined by the Etest method (BioMérieux, Marcy l’Etoile, France) on Difco GC agar until 2004 (Becton Dickinson, Cockeyswille, MD, USA) and after 2005 on chocolate agar with PolyViteX (BioMérieux) or on MH-horse blood agar (BioMérieux). In order to demonstrate the comparability of the different methods, we tested 9 isolates (1990–2004) and the reference ATCC strain 49226 with all three media for all four antibiotics (Additional file 1: Table S1). Beta-lactamase test was performed with nitrocefin (Becton Dickinson). The European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints were used : Breakpoints for the definition of decreased cefixime and ceftriaxone susceptibility were MICs >0.125 mg/L, and ciprofloxacin resistance was defined by MICs >0.064 mg/L. Penicillin resistance was defined by either MICs >1.0 mg/L or the presence of penicillinase-producing strains.
The following data was available for all isolates collected between 2005 and 2012: specimen source, date specimen obtained, sex, age, and specimen site. For all isolates collected before 2005 only date of specimen collection was available. The study was approved by the ethics committee of Zurich (ethics approval number: KEK-ZH-Nr. 2012–0510).
A total of 318 isolates from the region of Zurich and the northeastern part of Switzerland were analysed, including 42 samples from 1990, 66 from 2000 to 2004, and 210 samples from 2005 to 2012. The level of coverage (number of isolates tested compared to the number of reported cases in the region of Zurich) was 5%. Of the samples collected between 2005 and 2012, 168 (79.3%) were collected from men, 35 (16.5%) from women, and in 9 (4.2%) samples data on sex and age was missing. Median age for males was 35 years (range 17 to 86 years) and for females 31 years (range 16 to 66 years). The isolates were obtained from the urethra (60%), cervix (13%), other locations (4%), including anorectum, pharynx, joint and eye, or from unknown sites (24%).
N. gonorrhoeae with decreased susceptibility* to cefixime, ceftriaxone, and resistance to ciprofloxacin and penicillin in Switzerland, 1990–2012
DS, n (%)
DS, n (%)
R, n (%)
R, n (%)
Fluoroquinolone-resistant N. gonorrhoeae emerged in Switzerland during the 1990s, with a ciprofloxacin resistance rate of 2.4% in 1990. In the early 2000s, a rapid increase of ciprofloxacin resistance from 30.0% in 2001/2002 to 72.7% in 2011/2012 was observed (Table 1). Penicillin resistance rate was 22.7% in 2011/2012. However, only 18.2% of the strains were fully penicillin susceptible according to EUCAST breakpoints (≤0.06 mg/L).
In Switzerland, N. gonorrhoeae strains with decreased susceptibility to third-generation cephalosporins are emerging. We observed increasing rates of cefixime resistant strains, with a prevalence of 11% between 2007 and 2012. Moreover, ceftriaxone MICs are rising. The proportion of isolates with elevated ceftriaxone MIC was 2.2% between 2007 and 2012.
Our findings are in line with the worldwide documented decrease in cephalosporin susceptibility of N. gonorrhoeae. In the European Gonococcal antimicrobial susceptibility surveillance project (Euro-GASP), 9% of isolates displayed decreased susceptibility to cefixime (MIC ≥0.25 mg/L) in 2010. Cefixime resistance rates were >5% in 11 of 21 European countries, and >15% in 5 countries, including Austria, Denmark, Slovenia, Spain and Cyprus . In the United States the percentage of cefixime resistant isolates (MIC ≥0.25 mg/L) increased from 0.1% in 2006 to 1.4% in 2011 . The rise was most pronounced in the West with resistance rates of 17% in Hawaii and 6.4% in San Diego, California, in 2011 .
These are in vitro data and clinical cephalosporin resistance has not yet been defined due to the fact that so far there have been too few documented treatment failures in order to establish a clear relationship between MIC and clinical treatment failure. In a recently published Canadian cohort study the rate of clinical treatment failure associated with isolates of N. gonorrhoeae with a cefixime MIC of ≥0.12 mg/L was 25.0% compared to 1.9% of isolates with a cefixime MIC of <0.12 mg/L .
In 2008, we isolated one strain with a MIC for ceftriaxone of 0.25 mg/L. No decreased susceptibility to ceftriaxone (MIC ≥0.25 mg/L) was detected from 2004 to 2010 among Euro-GASP isolates. However, in correspondence to our data, ceftriaxone MICs increased from 2009 onwards . In the United States the percentage of isolates with elevated ceftriaxone MICs (≥0.125 mg/L) rose slightly from 0% in 2006 to 0.4% in 2011 . As displayed for cefixime, no threshold for resistance to ceftriaxone in N. gonorrhoeae has been defined yet. Increasing MICs for cephalosporins, however, may precede the emergence of resistance.
We found high ciprofloxacin resistance rates of N. gonorrhoeae, with 72.7% in 2012. Our data demonstrates that during the 1990s and early 2000s, when ciprofloxacin was first choice for treatment, fluoroquinolone-resistant isolates emerged, and rapidly increased after 2000, correlating with the observation in other countries around the world [24–26]. By 2007, in the United States, and even earlier in other regions, including Europe and Australia, fluoroquinolones were no longer recommended for the treatment of gonococcal infections [27, 28]. In Europe, ciprofloxacin resistance was 53% (MIC ≥1 mg/L) in 2010 , and 13.3% in the United States in 2011 (≥1 mg/L) .
The World Health Organization (WHO) recommends discontinuation of empirical use of an antibiotic once 5% of locally acquired gonococcal isolates are resistant . The high rates of strains with decreased cefixime susceptibility and first isolates with elevated MICs for ceftriaxone shown by our data, support the recommendation of dual therapy for gonococcal infections with ceftriaxone plus either azithromycin or doxycycline. Combination regimens have been adopted as strategies for the treatment of other bacterial infections in the context of multidrug resistance development. The oral cephalosporin, cefixime, should no longer be used as first-line treatment.
In light of identified strains with decreased ceftriaxone susceptibility, health-care providers need to be vigilant for treatment failures, even in patients treated with recommended antibiotic regimens. In persons treated with alternative regimens, such as cefixime, test of cure is recommended. Continued local surveillance of gonococcal antimicrobial susceptibility is important. Due to the widespread use of nucleic acid amplification testing, culture for N. gonorrhoeae with antimicrobial susceptibility testing is not routinely done in clinical practice.
To the best of our knowledge, this is the first report on cephalosporin susceptibility testing of N. gonorrhoeae in Switzerland. Longitudinal data on antimicrobial resistance in gonococci over a period of more than two decades was assessed. Nevertheless, the study has several limitations. The level of coverage was relatively low, attributed in a large part to the low number of gonococcal cultures taken routinely in clinical practice. The samples were not systematically collected within a sentinella system, which may result in selection of specimens with a higher resistance rate. Not all strains were tested for cephalosporins, which particularly concerns isolates obtained before 2006. However, the subset of 20-30% of all isolates stored, none showed decreased susceptibility for cefixime and ceftriaxone. It is therefore unlikely that we missed a relevant resistance signal. Finally, the isolates were not tested for azithromycin.
We found a high prevalence of N. gonorrhoeae with decreased susceptibility to cefixime, first strains with elevated MICs for ceftriaxone, and high resistance rates to ciprofloxacin. Treatment of gonorrhea with a combination regimen with ceftriaxone plus azithromycin or doxycycline is supported. Our data contributes to the global antimicrobial resistance surveillance of N. gonorrhoeae, and emphasizes the importance of further gonococcal antimicrobial resistance testing.
This study was supported in part by the University of Zurich. The authors thank J. Giger, R. Halter, G.Jost and the laboratory technicians for their dedicated help.
- Marrazzo JM, Handsfield HH, Sparling PF: Neisseria gonorrhoeae. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases. Edited by: Mandell GL, Bennett JE, Dolin R. 2010, Philadelphia: Churchill Livingstone Elsevier, 7Google Scholar
- Fleming DT, Wasserheit JN: From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection. Sex Transm Infect. 1999, 75 (1): 3-17. 10.1136/sti.75.1.3.View ArticlePubMedPubMed CentralGoogle Scholar
- Stoltey JE, Barry PM: The use of cephalosporins for gonorrhea: an update on the rising problem of resistance. Expert Opin Pharmacother. 2012, 13 (10): 1411-1420. 10.1517/14656566.2012.690396.View ArticlePubMedPubMed CentralGoogle Scholar
- Yokoi S, Deguchi T, Ozawa T, Yasuda M, Ito S, Kubota Y, Tamaki M, Maeda S: Threat to cefixime treatment for gonorrhea. Emerg Infect Dis. 2007, 13 (8): 1275-1277.PubMedPubMed CentralGoogle Scholar
- Lo JY, Ho KM, Leung AO, Tiu FS, Tsang GK, Lo AC, Tapsall JW: Ceftibuten resistance and treatment failure of Neisseria gonorrhoeae infection. Antimicrob Agents Chemother. 2008, 52 (10): 3564-3567. 10.1128/AAC.00198-08.View ArticlePubMedPubMed CentralGoogle Scholar
- Unemo M, Golparian D, Syversen G, Vestrheim DF, Moi H: Two cases of verified clinical failures using internationally recommended first-line cefixime for gonorrhoea treatment, Norway, 2010. Euro Surveill. 2010, 15 (47):Google Scholar
- Ison CA, Hussey J, Sankar KN, Evans J, Alexander S: Gonorrhoea treatment failures to cefixime and azithromycin in England, 2010. Euro Surveill. 2011, 16 (14):Google Scholar
- Unemo M, Golparian D, Stary A, Eigentler A: First Neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in Austria, 2011. Euro Surveill. 2011, 16 (43):Google Scholar
- Allen VG, Mitterni L, Seah C, Rebbapragada A, Martin IE, Lee C, Siebert H, Towns L, Melano RG, Low DE: Neisseria gonorrhoeae treatment failure and susceptibility to cefixime in Toronto, Canada. JAMA. 2013, 309 (2): 163-170. 10.1001/jama.2012.176575.View ArticlePubMedGoogle Scholar
- Lewis DA, Sriruttan C, Muller EE, Golparian D, Gumede L, Fick D, de Wet J, Maseko V, Coetzee J, Unemo M: Phenotypic and genetic characterization of the first two cases of extended-spectrum-cephalosporin-resistant Neisseria gonorrhoeae infection in South Africa and association with cefixime treatment failure. J Antimicrob Chemother. 2013, 68 (6): 1267-1270. 10.1093/jac/dkt034.View ArticlePubMedGoogle Scholar
- Tapsall J, Read P, Carmody C, Bourne C, Ray S, Limnios A, Sloots T, Whiley D: Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods. J Med Microbiol. 2009, 58 (Pt 5): 683-687.View ArticlePubMedGoogle Scholar
- Unemo M, Golparian D, Hestner A: Ceftriaxone treatment failure of pharyngeal gonorrhoea verified by international recommendations, Sweden, 2010. Euro Surveill. 2011, 16 (6):Google Scholar
- Ohnishi M, Golparian D, Shimuta K, Saika T, Hoshina S, Iwasaku K, Nakayama S, Kitawaki J, Unemo M: Is Neisseria gonorrhoeae initiating a future era of untreatable gonorrhea?: detailed characterization of the first strain with high-level resistance to ceftriaxone. Antimicrob Agents Chemother. 2011, 55 (7): 3538-3545. 10.1128/AAC.00325-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Unemo M, Golparian D, Nicholas R, Ohnishi M, Gallay A, Sednaoui P: High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure. Antimicrob Agents Chemother. 2012, 56 (3): 1273-1280. 10.1128/AAC.05760-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Camara J, Serra J, Ayats J, Bastida T, Carnicer-Pont D, Andreu A, Ardanuy C: Molecular characterization of two high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain. J Antimicrob Chemother. 2012, 67 (8): 1858-1860. 10.1093/jac/dks162.View ArticlePubMedGoogle Scholar
- Unemo M: The 2012 European guideline on the diagnosis and treatment of gonorrhoea in adults recommends dual antimicrobial therapy. Euro Surveill. 2012, 17 (47):Google Scholar
- Update to CDC’s Sexually transmitted diseases treatment guidelines, 2010: oral cephalosporins no longer a recommended treatment for gonococcal infections. MMWR Morb Mortal Wkly Rep. 2012, 61 (31): 590-594.Google Scholar
- Bundesamt für Gesundheit. 2013, http://www.bag.admin.ch/k_m_meldesystem/00733/00813/index.html?lang=de. Accessed May 4, 2013
- Le Lin B, Pastore R, Liassine N, Aramburu C, Sudre P: A new sexually transmitted infection (STI) in Geneva? Ciprofloxacin-resistant Neisseria gonorrhoeae, 2002–2005. Swiss Med Wkly. 2008, 138 (15–16): 243-246.PubMedGoogle Scholar
- World Health Organization (WHO): Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. http://whqlibdoc.who.int/publications/2012/9789241503501_eng.pdf,
- Breakpoint tablets for interpretation of MICs and zone diameters: version 3.1. European Committee on Antimicrobial Susceptibility Testing. http://www.eucast.org/clinical_breakpoints/. Accessed April 30
- Gonococcal antimicrobial susceptibility surveillance in Europe 2010 Stockholm: ECDC 2012:European Centre for Disease Prevention and Control (ECDC). 2010, Available from: http://ecdc.europa.eu/en/publications/Publications/1206-Gonococcal-AMR.pdf, Stockholm: ECDC 2012:European Centre for Disease Prevention and Control (ECDC),
- CDC Grand rounds: the growing threat of multidrug-resistant gonorrhea. MMWR Morb Mortal Wkly Rep. 2013, 62 (6): 103-106.Google Scholar
- Fenton KA, Ison C, Johnson AP, Rudd E, Soltani M, Martin I, Nichols T, Livermore DM: Ciprofloxacin resistance in Neisseria gonorrhoeae in England and Wales in 2002. Lancet. 2003, 361 (9372): 1867-1869. 10.1016/S0140-6736(03)13489-7.View ArticlePubMedGoogle Scholar
- Tanaka M, Nakayama H, Notomi T, Irie S, Tsunoda Y, Okadome A, Saika T, Kobayashi I: Antimicrobial resistance of Neisseria gonorrhoeae in Japan, 1993–2002: continuous increasing of ciprofloxacin-resistant isolates. Int J Antimicrob Agents. 2004, 24 (Suppl 1): S15-22.View ArticlePubMedGoogle Scholar
- Hsueh PR, Tseng SP, Teng LJ, Ho SW: High prevalence of ciprofloxacin-resistant Neisseria gonorrhoeae in Northern Taiwan. Clin Infect Dis. 2005, 40 (1): 188-192. 10.1086/426142.View ArticlePubMedGoogle Scholar
- Update to CDC’s sexually transmitted diseases treatment guidelines, 2006: fluoroquinolones no longer recommended for treatment of gonococcal infections. MMWR Morb Mortal Wkly Rep. 2007, 56 (14): 332-336.Google Scholar
- Tapsall JW, Limnios EA, Murphy D: Analysis of trends in antimicrobial resistance in Neisseria gonorrhoeae isolated in Australia, 1997 2006. J Antimicrob Chemother. 2008, 61 (1): 150-155.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/13/603/prepub
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