This article has Open Peer Review reports available.
Effectiveness of treatment with pegylated interferon and ribavirin in an unselected population of patients with chronic hepatitis C: A Danish nationwide cohort study
© Hansen et al; licensee BioMed Central Ltd. 2011
Received: 11 December 2010
Accepted: 21 June 2011
Published: 21 June 2011
The effect of peginterferon and ribavirin treatment on chronic hepatitis C virus (HCV) infection has been established in several controlled clinical studies. However, the effectiveness of treatment and predictors of treatment success in routine clinical practice remains to be established. Our aim was to estimate the effectiveness of peginterferon and ribavirin treatment in unselected HCV patients handled in routine clinical practice. The endpoint was sustained virological response (SVR), determined by the absence of HCV RNA 24 weeks after the end of treatment.
We determined the proportion of SVR in a nationwide, population-based cohort of 432 patients with chronic HCV infection who were starting treatment, and analyzed the impact of known covariates on SVR by using a logistic regression analysis.
The majority of treated patients had genotype 1 (133 patients) and genotype 2/3 (285 patients) infections, with 44% and 72%, respectively, obtaining SVR. Other than genotype, the predictors of SVR were age ≤ 45 years at the start of treatment, completion of unmodified treatment, the absence of cirrhosis and non-European origin.
The effectiveness of peginterferon and ribavirin treatment for chronic hepatitis C in a routine clinical practice is comparable to that observed in controlled clinical trials, with a higher SVR rate in genotype 2 and 3 patients compared to genotype 1 patients. Our data further indicate that age at start of treatment is a strong predictor of SVR irrespective of HCV genotype, with patients 45 years or younger having a higher SVR rate.
Combination therapy with peginterferon and ribavirin has improved treatment response in patients with chronic hepatitis C virus (HCV) infection, but the virus is still only eradicated in 50-80% of the patients receiving treatment [1–3], depending on HCV genotype. Treatment is lengthy and has severe side effects, which may lead to dose reduction or even prevent treatment completion. There are also several contraindications to starting treatment, such as ongoing psychiatric disease or active intravenous drug use (IDU). Progress has been made in the development of new direct-acting antiviral drugs, specifically targeting viral replication, and some of these antivirals have recently been studied in clinical trials. The results are promising especially for certain protease inhibitors expected to be licensed soon for the treatment of chronic HCV infection, as part of triple therapy with peginterferon and ribavirin . It is, however, still uncertain when these new agents will be introduced into routine clinical practice.
Most previous studies of antiviral treatment response in HCV infected patients have included specific and often highly selected groups of patients (patients in clinical trials, in tertiary hospitals etc.), and these studies are therefore prone to be affected by selection bias. It was found that female gender, young age, being infected with genotype 2 or 3, absence of cirrhosis, Asian origin and early inhibition of viral replication predict a better response to treatment [5–17]. It is however, controversial whether the promising treatment effects observed in clinical trials can be transformed into an equivalent effectiveness in a routine clinical setting, although one retrospective observational cohort study by Backus et al. of patients treated in a routine clinical setting at Veterans Affairs facilities showed efficacy of treatment to be 20-52% .
The aim of the present study was to estimate the effectiveness of treatment for chronic HCV infection with peginterferon and ribavirin in a nationwide, population based cohort of HCV infected patients.
The prevalence of chronic HCV infection in Denmark was estimated by the Danish National Board of Health to be around 0.3% (15,000 individuals) . Approximately 25% of these individuals are, or have been, monitored in specialized hospital departments, where antiviral therapy for HCV infection is provided for all patients found to be in need of treatment.
The Danish Database for Hepatitis B and C (DANHEP)
DANHEP was established in January 2002 as a nationwide open cohort study with ongoing enrolment. It includes data about individuals in Denmark who are chronically infected with hepatitis B and/or C, are 16 years of age or older, are followed in one of the 11 medical departments that monitor and treat patients with chronic hepatitis in Denmark, and have been seen at least once after 1 January 2002 in one of these departments. The database is described in detail elsewhere .
Tests for HCV RNA and HCV genotype
For 9 of the 11 departments recruiting patients to the DANHEP study, serum HCV-RNA was quantified in one laboratory by a previously described RT-PCR method with a detection limit of 20 IU/mL . Here, HCV-genotype was determined in RT-PCR with genotype specific primers from the 5' non-coding region of the virus genome . The remaining two departments in DANHEP used either COBAS AmpliPrep/COBAS TaqMan HCV test (Roche diagnostics, Branchburg, NJ) (detection limit 15 IU/mL), COBAS Amplicor HCV Monitor (Roche diagnostics) (detection limit 300 IU/mL) or qualitative HCV-RNA measurements (detection limit 50 IU/mL) to quantify HCV-RNA and the Versant HCV Genotyping Assay, LiPA (Bayer HealthCare LCC) to determine the HCV genotype. Here, genotype was also, in some cases analyzed using conserved primers deduced from the core-envelope 1-region of the virus in RT-PCR followed by sequence analysis . All samples tested for the presence of HCV RNA 24 weeks after the end of treatment were tested using one of the methods with detection limit of 50 IU/mL or lower.
Although HCV genotype 6 is not found in Danish patients , it is widely present in Asia. We therefore re-analyzed Asian patients found to be infected with genotype 1, if the genotype was determined in RT-PCR with primers from the HCV 5' non-coding region, because this is not a valid method to distinguish genotype 1 from 6. The re-analysis was performed in RT-PCR of the Core-E1 region and the NS5B-region followed by sequence analysis of the resulting amplicons [22, 23].
Baseline characteristics of 432 patients chronically infected with HCV, treated with peginterferon and ribavirin in Denmark between 1 January 2002 and 1 January 2007
Route of infection
Age at treatment initiation
≤ 45 years
> 600.000 IU/mL
2 × UNL*
Liver biopsy within 3 years
With dose reduction
Ended before scheduled
Treatment with either pegylated interferon alpha-2a (Pegasys®, Roche A/S, Basel, Switzerland) or alpha-2b (PegIntron®, Schering-Plough, Kenilworth, NJ, USA) was given weekly according to the manufacturer's instructions. Ribavirin (Copegus®, Roche or Rebetol®, Schering-Plough) was given daily, adjusted to body weight for genotypes 1 and 4 or as "flat dosage" for genotypes 2 and 3, according to the manufacturer's instructions. The duration of therapy was planned for 48 weeks for genotype 1 and 4 and 24 weeks for genotype 2 and 3. Some patients with genotype 2 or 3 who were included in a clinical trial were randomized to 12 weeks of treatment . Other genotype 2 or 3 infected patients participating in another clinical trial were randomized to 14 or 24 weeks of treatment, if they had a negative HCV-RNA after 4 weeks . In general, if HCV-RNA titers had not decreased by a minimum of 2 logs after 12 weeks of treatment, treatment was stopped according to international guidelines.
Treatment outcome was evaluated 24 weeks after end of treatment, where patients with undetectable HCV-RNA were categorized as responders, having achieved sustained virological response (SVR). Patients with detectable HCV-RNA levels 24 weeks after end of treatment were categorized as non-responders.
Unadjusted and adjusted odds ratios and 95% confidence intervals (CI) for sustained virological response after treatment with peginterferon and ribavirin in 432 chronically HCV infected patients, according to predictor variables
Odds ratio (95% CI)
Odds ratio (95% CI)
Route of infection
Age ≤ 45 years at treatment initiation
Age > 45 years at treatment initiation
2 or 3
≤ 600.000 IU/mL
> 600.000 IU/mL
Less than 2 × UNL*
More than 2 × UNL
Treating department (speciality)
0.44 (0.25-0.79) §
With dose reduction
Ended before scheduled
Previous studies have shown that patients infected with genotype 2/3 have a higher response rate to combination therapy, and we therefore performed the analysis stratified on HCV genotype (genotype 1 vs. genotype 2/3). Because only 10 and 2 patients were infected with HCV genotype 4 and 6, respectively, these patients were not included in the analyses stratified on HCV genotype.
Starting treatment at a younger age has been associated with higher SVR rates [1, 6, 8, 9, 25]. Some studies have included age as a dichotomized variable, while others have included age in the models as a continuous variable. We divided the cohort according to age in 10 groups of equal size and made logistic regression analyses to estimate beta values, to evaluate whether to use a continuous age scale or a specific cut-off point and if so, to estimate the best cut-off point. We observed a sharp drop in beta values between the ages of 43-47, and therefore age was modeled in the regression analysis as equal to or less than 45 vs. over 45 years of age at start of treatment.
Data analyses were performed using SPSS statistical analysis version 16.0 (SPSS, Chicago, Illinois, USA). The Study was approved by the Danish Data Protection Agency (J. nr. 2007-41-0815).
Study population characteristics
In total, 432 chronically infected HCV patients were included in the study (table 1). Among the 372 patients of European origin, 327 (87.9%) were Danish. The group of non-Europeans consisted of 10 African-, 3 American-, 9 Asian-, 15 Pakistani-, and 15 Middle Eastern patients. Eight patients had an unknown country of origin. Median age at treatment initiation was 46 years, (range 20-71). Twenty-eight patients (6.5%) received lower start doses of peginterferon, ribavirin, or both, than recommended by the manufacturer.
Genotyping revealed that 133 (30.8%) patients were infected with genotype 1, 50 (11.6%) with genotype 2, 235 (54.4%) with genotype 3, 10 (2.3%) with genotype 4, and 2 patients with genotype 6 (0.5%). In 2 patients (0.5%) the HCV genotype was unknown.
Effectiveness of treatment with pegylated interferon and ribavirin and predictors of SVR
The overall SVR rate was 63%, with rates of 44%, 74%, 71% and 60% for patients with genotypes 1, 2, 3 and 4, respectively (table 1). Both patients with genotype 6 infection obtained SVR.
Odds ratios and 95% confidence intervals (CI) for age as predictor of sustained virological response after treatment with peginterferon and ribavirin in 418 chronically HCV infected patients, divided by genotype (133 genotype 1, 285 genotype 2/3)
Odds ratio (95% CI)*
Odds ratio (95% CI)*
≤ 45 years
> 45 years
Odds ratios and 95% confidence intervals (CI) for genotype as predictor of sustained virological response after treatment with peginterferon and ribavirin in 432 chronically HCV infected patients, divided by age
Age ≤ 45 years
Age > 45 years
Odds ratio (95% CI)*
Odds ratio (95% CI)*
In an analysis only including patients who had had a liver biopsy performed prior to treatment initiation, we found that cirrhosis was negatively associated with SVR, but the effect became statistically insignificant after adjustment of other co-factors (table 2).
Data available after treatment initiation showed that dose reduction or stopping treatment before scheduled were strongly associated with a reduced chance of SVR (table 2).
In a nationwide population based cohort of chronically infected HCV patients we observed sustained virological response (SVR) rates of 44% and 72% for genotype 1 and 2/3 respectively, after combination therapy with peginterferon and ribavirin. Other main predictors of SVR were age ≤ 45 years at treatment initiation and completion of non-modified treatment regimens. Finally, being of Non-European nationality predicted a higher SVR rate.
While several controlled clinical trials have evaluated effectiveness of treatment with peginterferon and ribavirin in HCV infected patients, the effectiveness of treatment in a routine clinical setting is less well shown [10, 25], except for one retrospective, observational cohort study by Backus et al. with SVR rates of 20%, 52% and 43% in genotypes 1, 2 and 3, respectively, although the patients included in this study were mainly men . However, the present study demonstrated that the effectiveness of treatment, with SVR rates of 44-72%, is comparable to the SVR rates reported in controlled, clinical trials of genotype 1-3 patients [1, 2]. The unique perspective of the present study is the nationwide, population based cohort design and a relatively large number of genotype 1, 2 and 3 infected patients included with an almost even distribution of males and females. As only very few patients in Denmark are treated at medical departments not participating in the DANHEP study, our study includes the vast majority of patients treated for HCV in Denmark during the study period.
In accordance with observations obtained in controlled, clinical trials we found both genotype and younger age to be predictors of SVR in patients treated with peginterferon and ribavirin [1, 5, 6, 25], but to our knowledge the present study is the only one, based on a nationwide cohort of unselected patients evenly distributed between males and females, treated in a routine clinical setting, thus representing an important confirmation of the effectiveness of treatment with pegylated interferon and ribavirin observed in controlled, clinical trials. In the present study, infection with genotype 2/3 was a strong predictor of SVR, also in patients ≤ 45 years, though statistical significance was not shown in this group.
In a study by Elefsiniotis et al. , genotype was not found as a predictor of SVR in patients younger than 35 years, and age was not a significant predictor of SVR in the overall cohort. However, in their analysis, age was used as a continuous variable. We have not been able to determine why certain cut off points for age have been chosen in other studies evaluating age as a predictive factor of SVR. In the present study, we evaluated whether or not age was best modeled as a continuous variable, or as a design variable dividing age in two groups. We found that the age of 45 was a relevant cut off point.
Even though Antonucci et al.  only included half as many patients with genotype 1 and a third as many with genotype 2/3, as we did, in accordance with our results, they found that age of < 40 years was a significant predictor of SVR in genotype 1 infected patients treated outside clinical trials. Similarly to Antonucci et al, we did not find younger age to be a statistically significant predictor of SVR in genotype 2/3 infected patients. In our study however, there was a trend towards younger age also being a predictor of SVR in genotype 2/3 (OR: 0.58, 95% CI: 0.33-1.00). Dalgard et al.  and Lagging et al. , included 428 and 382 highly selected genotype 2/3 patients, respectively, and found that younger age predicted a higher chance of SVR. Older age at the time of infection is known to be associated with development of liver fibrosis [26–28]. Patients are carefully selected before initiation of antiviral treatment, because the overall treatment outcome is "poor", and treatment is lengthy, with many potentially severe side effects. International guidelines on whom to treat suggest that there should be evidence of liver fibrosis before treatment initiation in genotype 1 patients [29, 30]. Due to the better treatment outcome and shorter treatment duration, patients infected with genotype 2 or 3 can start treatment without prior liver biopsy.
A former study, based on data from DANHEP , showed that patients infected with genotype 2/3, had an approximately 2.5 times higher chance of starting antiviral treatment, while older age at referral was a negative predictor of treatment initiation. The results in the present study, with relatively high SVR rates in genotype 1 patients age 45 or younger, could have implications for future treatment strategy in this group of patients. Whether younger patients undergoing the new upcoming treatment regimens that add a specific HCV protease inhibitor to the standard of care treatment, can benefit from even shorter treatment periods remains to be seen. Clearly, further studies are required to establish the specific indications for treatment initiation in younger patients. Being infected with HCV has been shown to decrease quality of life, regardless of disease progression, and SVR after treatment improves quality of life . This is another aspect that should be considered when deciding whether or not to treat patients for chronic hepatitis C.
Recently, a genetic variation near the IL28B gene on chromosome 19 was found to be significantly associated with SVR in HCV infected genotype 1 patients, treated with peginterferon and ribavirin [32–34]. Ge et al.  found this advantageous genetic variation in 35% of the cohort examined. Patients with this variation had a 2-3 fold higher rate of SVR than other patients. Around 25-40% of the patients without the advantageous genetic variation gained SVR, and the genetic variation was only associated with an SVR in patients who did not become HCV RNA negative at week 4 in patients with genotypes 2 and 3 . Therefore it is still important to identify and use other predictors of SVR when deciding whether to start treatment for HCV infection. Genetics do not change with age, therefore the main findings of the present study are still very important. It is possible that patients with genotype 1 HCV infection without the advantageous IL28B genetic variation, identified in the studies mentioned above, could benefit from treatment before the age of 45 years.
The association between being of non-European origin and the high SVR rate could not be explained from our data. Thus, further research is needed to elucidate whether this difference could potentially be related to IL28B polymorphism differences, as the group of non-Europeans was diverse ethnically and data on IL28B-polymorphisms do not exist for all ethnicities involved in this study.
There are some limitations to the present study. The study is a retrospective observational study, and we are lacking information on some potentially important confounders such as weight, prior depression, and prior raised alcohol intake. We did not have any information on viral kinetics during treatment, and therefore we could not include this factor in the analyses. Furthermore, we did not have any knowledge of which patients were treated in clinical trials. It could have been interesting to determine if being treated in a clinical trial was a predictor of SVR.
We conclude that in a routine clinical practice the treatment effectiveness of patients with chronic HCV with peginterferon and ribavirin is equivalent to the effectiveness observed in clinical trials. Furthermore, genotype 2 and 3, age ≤ 45 years at treatment initiation and completion of a non-modified treatment regimen is strongly associated with a sustained treatment response. Thus, our study on a nationwide Danish cohort shed new light on combination therapy of chronic HCV.
Potential conflict of interest
Peer Brehm Christensen is member of the Advisory Board for Roche A/S and has received research grants from Roche A/S and Sheering Plough.
Nanna Hansen; No conflict, Niels Obel; No conflict, Mette Kjær; No conflict, Alex Lund Laursen; No conflict, Henrik Bygum Krarup; No conflict, Axel Møller; No conflict, Poul Schlichting; No conflict, Jens Bukh; No conflict, Nina Weis; No conflict.
No authors listed in appendix 1 have any conflict of interest
Acknowledgements and Funding
Additional members of the DANHEP group:
Hans Kromann-Andersen, Department of Gastroenterology, Koege University Hospital, Suzanne Lunding, Department of Internal Medicine, Helsingoer Hospital, Karin Groenbaek, Department of Gastroenterology, Copenhagen University Hospital, Hvidovre, Peter Noerregaard, Department of Internal Medicine, Frederiksberg Hospital, Kim Krogsgaard, Copenhagen University Hospital, Hvidovre, Jens Lindberg, Department of Internal Medicine, Herning Hospital, Henrik Nielsen, Department of Infectious Diseases, Aalborg University Hospital, Jens Ole Nielsen, Department of Infectious Diseases, Copenhagen University Hospital, Hvidovre, Ove B. Schaffalitzky de Muckadell, Department of Gastroenterology, Odense University Hospital.
We are grateful to the staff of the participating clinical departments for their continuous support of recording data in DANHEP. We also thank statistician Steen Ladelund, Clinical Research Centre, Copenhagen University Hospital, Hvidovre, Denmark, for statistical advice.
This study was supported by The Lundbeck Foundation (grant support to Jens Bukh) and the A.P. Møller Foundation for the Advancement of Medical Science (grant support to Nina Weis).
- Manns MP, McHutchison JG, Gordon SC, Rustgi VK, Shiffman M, Reindollar R, Goodman ZD, Koury K, Ling M, Albrecht JK: Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet. 2001, 358: 958-65. 10.1016/S0140-6736(01)06102-5.View ArticlePubMedGoogle Scholar
- Fried MW, Shiffman ML, Reddy KR, Smith C, Marinos G, Goncales FL, Häussinger D, Diago M, Carosi G, Dhumeaux D, Craxi A, Lin A, Hoffman J, Yu J: Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. N Engl J Med. 2002, 347: 975-82. 10.1056/NEJMoa020047.View ArticlePubMedGoogle Scholar
- Hadziyannis SJ, Sette H, Morgan TR, Balan V, Diago M, Marcellin P, Ramadori G, Bodenheimer H, Bernstein D, Rizzetto M, Zeuzem S, Pockros PJ, Lin A, Ackrill AM, PEGASY International Study Group: Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose. Ann Intern Med. 2004, 140: 346-55.View ArticlePubMedGoogle Scholar
- Hofmann WP, Zeuzem S: A new standard of care for the treatment of chronic HCV infection. Nat Rev Gastroenterol Hepatol. 2011, Epub April 5Google Scholar
- Kau A, Vermehren J, Sarrazin C: Treatment predictors of a sustained virologic response in hepatitis B and C. J Hepatol. 2008, 49: 634-51. 10.1016/j.jhep.2008.07.013.View ArticlePubMedGoogle Scholar
- Foster GR, Fried MW, Hadziyannis SJ, Messinger D, Freivogel K, Weiland O: Prediction of sustained virological response in chronic hepatitis C patients treated with peginterferon alfa-2a (40KD) and ribavirin. Scand J Gastroenterol. 2007, 42: 247-55. 10.1080/00365520600955526.View ArticlePubMedGoogle Scholar
- Zeuzem S, Buti M, Ferenci P, Sperl J, Horsmans Y, Cianciara J, Ibranyi E, Weiland O, Noviello S, Brass C, Albrecht J: Efficacy of 24 weeks treatment with peginterferon alfa-2b plus ribavirin in patients with chronic hepatitis C infected with genotype 1 and low pretreatment viremia. J Hepatol. 2006, 44: 97-103. 10.1016/j.jhep.2005.10.003.View ArticlePubMedGoogle Scholar
- Dalgard O, Bjoro K, Ring-Larsen H, Bjornsson E, Holberg-Petersen M, Skovlund E, Reichard O, Myrvang B, Sundelöf B, Ritland S, Hellum K, Frydén A, Florholmen J, Verbaan H, North-C group: Pegylated interferon alfa and ribavirin for 14 versus 24 weeks in patients with hepatitis C virus genotype 2 or 3 and rapid virological response. Hepatology. 2008, 47: 35-42.View ArticlePubMedGoogle Scholar
- Lagging M, Langeland N, Pedersen C, Farkkila M, Buhl MR, Morch K, Dhillon AP, Alsiö A, Hellstrand K, Westin J, Norkrans G, NORDynamIC Study Group: Randomized comparison of 12 or 24 weeks of peginterferon alpha-2a and ribavirin in chronic hepatitis C virus genotype 2/3 infection. Hepatology. 2008, 47: 1837-45. 10.1002/hep.22253.View ArticlePubMedGoogle Scholar
- Elefsiniotis IS, Pavlidis C, Ketikoglou I, Koutsounas S, Scarmeas N, Pantazis KD, Moulakakis A, Tsianos EV: Patient's age modifies the impact of the proposed predictors of sustained virological response in chronic hepatitis C patients treated with PEG-interferon plus ribavirin. Eur J Intern Med. 2008, 19: 266-70. 10.1016/j.ejim.2007.06.014.View ArticlePubMedGoogle Scholar
- Missiha S, Heathcote J, Arenovich T, Khan K: Impact of asian race on response to combination therapy with peginterferon alfa-2a and ribavirin in chronic hepatitis C. Am J Gastroenterol. 2007, 102: 2181-8. 10.1111/j.1572-0241.2007.01431.x.View ArticlePubMedGoogle Scholar
- Backus LI, Boothroyd DB, Phillips BR, Mole LA: Predictors of response of US veterans to treatment for the hepatitis C virus. Hepatology. 2007, 46: 37-47.View ArticlePubMedGoogle Scholar
- Shiffman ML, Suter F, Bacon BR, Nelson D, Harley H, Sola R, Shafran SD, Barange K, Lin A, Soman A, Zeuzem S: Peginterferon alfa-2a and ribavirin for 16 or 24 weeks in HCV genotype 2 or 3. N Engl J Med. 2007, 357: 124-34. 10.1056/NEJMoa066403.View ArticlePubMedGoogle Scholar
- Shirakawa H, Matsumoto A, Joshita S, Komatsu M, Tanaka N, Umemura T, Ichijo T, Yoshizawa K, Kiyosawa K, Tanaka E: Pretreatment prediction of virological response to peginterferon plus ribavirin therapy in chronic hepatitis C patients using viral and host factors. Hepatology. 2008, 48: 1753-60. 10.1002/hep.22543.View ArticlePubMedGoogle Scholar
- Mangia A, Santoro R, Minerva N, Ricci GL, Carretta V, Persico M, Vinelli F, Scotto G, Bacca D, Annese M, Romano M, Zechini F, Sogari F, Spirito F, Andriulli A: Peginterferon alfa-2b and ribavirin for 12 vs. 24 weeks in HCV genotype 2 or 3. N Engl J Med. 2005, 352: 2609-17. 10.1056/NEJMoa042608.View ArticlePubMedGoogle Scholar
- Lagging M, Langeland N, Pedersen C, Farkkila M, Buhl MR, Morch K, Dhillon AP, Alsiö A, Hellstrand K, Westin J, Christensen P, Leutscher P, Norkrans G: Weight-adjusted dosing of ribavirin and importance of hepatitis C virus RNA below 1000 IU/mL by day 7 in short-term peginterferon therapy for chronic genotype 2/3 hepatitis C virus infection. Hepatology. 2008, 48: 695-View ArticlePubMedGoogle Scholar
- Loustaud-Ratti V, Alain S, Rousseau A, Hubert IF, Sauvage FL, Marquet P, Denis F, Lunel F, Calès P, Lefebvre A, Fauchais AL, Liozon E, Vidal E: Ribavirin exposure after the first dose is predictive of sustained virological response in chronic hepatitis C. Hepatology. 2008, 47: 1453-61. 10.1002/hep.22217.View ArticlePubMedGoogle Scholar
- Danmarks statistik. 2008, Ref Type: Internet Communication, [http://www.dst.dk]
- Hansen N, Obel N, Christensen PB, Krarup H, Laursen AL, Clausen MR, Lunding S, Møller A, Schlichting P, Kromann-Andersen H, Bukh J, Weis N: Predictors of antiviral treatment initiation in hepatitis C virus-infected patients: a Danish cohort study. J Viral Hepat. 2009, 16: 659-65. 10.1111/j.1365-2893.2009.01126.x.View ArticlePubMedGoogle Scholar
- Krarup HB, Drewes AM, Madsen PH: A quantitative HCV-PCR test for routine diagnostics. Scand J Clin Lab Invest. 1998, 58: 415-22. 10.1080/00365519850186391.View ArticlePubMedGoogle Scholar
- Krarup HB, Moller JM, Christensen PB, Fuglsang T, Ingerslev J, Arnfred T, Madsen PH: Haemophilic patients with hepatitis C have higher viral load compared to other well-defined patient groups. J Viral Hepat. 2000, 7: 435-9. 10.1046/j.1365-2893.2000.00258.x.View ArticlePubMedGoogle Scholar
- Corbet S, Bukh J, Heinsen A, Fomsgaard A: Hepatitis C virus subtyping by a core-envelope 1-based reverse transcriptase PCR assay with sequencing and its use in determining subtype distribution among Danish patients. J Clin Microbiol. 2003, 41: 1091-100. 10.1128/JCM.41.3.1091-1100.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Laperche S, Le Marrec N, Girault A, Bouchardeau F, Servant-Delmas A, Maniez-Montreuil M, Gallian P, Levayer T, Morel P, Simon N: Simultaneous detection of hepatitis C virus (HCV) core antigen and anti-HCV antibodies improves the early detection of HCV infection. J Clin Microbiol. 2005, 43: 3877-83. 10.1128/JCM.43.8.3877-3883.2005.View ArticlePubMedPubMed CentralGoogle Scholar
- Bedossa P, Poynard T: An algorithm for the grading of activity in chronic hepatitis C. The METAVIR Cooperative Study Group. Hepatology. 1996, 24: 289-93. 10.1002/hep.510240201.View ArticlePubMedGoogle Scholar
- Antonucci G, Longo MA, Angeletti C, Vairo F, Oliva A, Comandini UV, Tocci G, Boumis E, Noto P, Solmone MC, Capobianchi MR, Girardi E: The effect of age on response to therapy with peginterferon alpha plus ribavirin in a cohort of patients with chronic HCV hepatitis including subjects older than 65 yr. Am J Gastroenterol. 2007, 102: 1383-91. 10.1111/j.1572-0241.2007.01201.x.View ArticlePubMedGoogle Scholar
- Marcellin P, Asselah T, Boyer N: Fibrosis and disease progression in hepatitis C. Hepatology. 2002, 36 (5 Suppl 1): S47-S56.View ArticlePubMedGoogle Scholar
- Yano M, Kumada H, Kage M, Ikeda K, Shimamatsu K, Inoue O, Hashimoto E, Lefkowitch JH, Ludwig J, Okuda K: The long-term pathological evolution of chronic hepatitis C. Hepatology. 1996, 23: 1334-40. 10.1002/hep.510230607.View ArticlePubMedGoogle Scholar
- Chen SL, Morgan TR: The natural history of hepatitis C virus (HCV) infection. Int J Med Sci. 2006, 3: 47-52.View ArticlePubMedPubMed CentralGoogle Scholar
- Strader DB, Wright T, Thomas DL, Seeff LB: Diagnosis, management, and treatment of hepatitis C. Hepatology. 2004, 39: 1147-71. 10.1002/hep.20119.View ArticlePubMedGoogle Scholar
- Wejstal R, Alaeus A, Fischler B, Reichard O, Uhnoo I, Weiland O: Chronic hepatitis C: updated Swedish consensus. Scand J Infect Dis. 2003, 35: 445-51. 10.1080/00365540310013252.View ArticlePubMedGoogle Scholar
- Younossi Z, Kallman J, Kincaid J: The effects of HCV infection and management on health-related quality of life. Hepatology. 2007, 45: 806-16. 10.1002/hep.21565.View ArticlePubMedGoogle Scholar
- Ge D, Fellay J, Thompson AJ, Simon JS, Shianna KV, Urban TJ, Heinzen EL, Qui P, Bertelsen AH, Muir AJ, Sulkowski M, McHutchison JG, Goldstein DB: Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature. 2009, 461: 399-401. 10.1038/nature08309.View ArticlePubMedGoogle Scholar
- Suppiah V, Moldovan M, Ahlenstiel G, Berg T, Weltman M, Abate ML, Bassendine M, Spengler U, Dore GJ, Powell E, Riordan S, Sheridan D, Smedile A, Fragomeli V, Mûller T, Bahlo M, Stewart GJ, Booth DR, George J: IL28B is associated with response to chronic hepatitis C interferon-alpha and ribavirin therapy. Nat Genet. 2009, 41: 1100-4. 10.1038/ng.447.View ArticlePubMedGoogle Scholar
- Tanaka Y, Nishida N, Sugiyama M, Kurosaki M, Matsuura K, Sakamoto N, Nakagawa M, Korenaga M, Hino K, Hige S, Ito Y, Mita E, Tanaka E, Mochida S, Murawaki Y, Honda M, Sakai A, Hiasa Y, Nishiguchi S, Koike A, Sakaida I, Imamura M, Ito K, Yano K, Masaki N, Sugauchi F, Izumi N, Tokunaga K, Mizokami M: Genome-wide association of IL28B with response to pegylated interferon-alpha and ribavirin therapy for chronic hepatitis C. Nat Genet. 2009, 41: 1105-9. 10.1038/ng.449.View ArticlePubMedGoogle Scholar
- Mangia A, Thompson AJ, Santoro R, Piazolla V, Tillmann HL, Patel K, Shianna KV, Mottola L, Petruzzellis D, Bacca D, Carretta V, Minerva N, Goldstein DB, McHutchison JG: An IL28B polymorphism determines treatment response of hepatitis C virus genotype 2 or 3 patients who do not achieve a rapid virological response. Gastroenterology. 2010, 130: 821-27.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/11/177/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.