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A novel internet sampling for HIV surveillance: feasibility of self-sampling and preparation of DBS for delivery detection of HIV total nucleic acid and complementarity to sentinel surveillance



To propose a new mode of HIV test and surveillance among population of men who have sex with men (MSM): Internet-based Self-sampling at home plus Laboratory testing of HIV total nucleic acid (TNA) in dried blood spot (DBS) (ISL of DBS TNA). Feasibility of ISL of DBS TNA was studied. Characteristics of the new mode and that of conventional surveillance mode at HIV voluntary counseling and testing clinic (VCT) were compared.


A non-governmental organization (NGO) published the recruitment information on the WeChat public account. MSM filled in the questionnaire online, applied for self-sampling service package, and mailed the self made DBS to professional laboratory. The laboratory performed HIV TNA test and submitted the test results to online platform. Participants queried test results online with their unique ID. Center for Disease Control and Prevention (CDC) followed up participants with positive nucleic acid results using IDs and contact information. Rates were compared by using the Chi-Square test or Fisher's exact test.


Four hundred twenty-three questionnaires were completed. 423 self-sampling service packages were sent out and 340 DBSs were returned to professional laboratory within one month with qualified rate of sampling as high as 95.0% (323/340). Seven samples were found to be TNA positive. Comparing ISL of DBS TNA with sentinel surveillance, it was found that there was a significant difference in the composition ratio of the two modes of surveillance population (P < 0.05). HIV prevalence of ISL of DBS as 2.17% was significantly lower than sentinel site as 8.96% (χ2 = 14.953, P = 0.000 < 0.05).


ISL of DBS TNA proposed is feasible and has a high self-sampling qualification rate, good confidentiality. It is an effective supplement to routine sentinel surveillance and has important promotion value.

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The AIDS epidemic in China is still very severe. According to Chinese Center for Disease Control and Prevention, the survival number of HIV-infected patients was estimated at 850,000 by the end of September 2018. However, 30% of the infected persons are still undetected [1]. Low detection rate is the key bottleneck to contain the AIDS epidemic in China. High-risk sexual behavior of infected persons led to the vast majority of new HIV transmission, producing a new round of HIV infection cases. Studies show that 50% of new infections come from the second generation transmission of early infected patients [2,3,4]. Acute infections are estimated to be 26 times more infectious than asymptomatic infections [5, 6]. Initial viral load is up to 106-107cp / ml, which is highly infectious. The HIV infected by MSM is mainly a CXCR4 tropical strain and is highly pathogenic. The average incubation period is only 4–5 years, significantly shorter than the 8–10 years incubation period of other strains [7]. The infections need to be detected and treated as soon as possible. However, many infected people failed to receive HIV test timely, but were found late in the hospital due to various complications when the immune system was seriously damaged, resulting in high mortality rate. Sexual transmission is the main route of AIDS transmission in China. Heterosexual and male homosexual transmission accounted for 69.6% and 25.5% of reported infections in 2017, respectively [8].Men who have sex with men (MSM) are at high risk of HIV infection. In Beijing, MSM is the main transmission route [9], with HIV infection rate 100–400 times higher than general population and also higher than drug users (9.08%) and sex workers (0.36%) [10,11,12]. In addition, concealment of this population makes both its discovery and intervention very difficult. How to improve the detection accessibility of MSM population has become the key to control its HIV infection. Fear of privacy exposure is a major reason of reluctance to obtain HIV face-to-face testing with medical staff. Internet-based Self-sampling at home plus Laboratory testing (ISL) is anonymous and does not have to provide services face to face, and the examiners do not have concerns about privacy exposure, which will become an important means for the general intervention of the high-risk groups of HIV infection in the future. By now, HIV test mode of internet platform plus urine sampling at home plus antibody testing by professional laboratory has achieved phased success in Beijing [13], the urine detection sensitivity is about 90%, and the window period is about one month [14]. Compared with urine antibody testing, nucleic acid commercial testing kits perform better, with a sensitivity of up to 99% [15] and a window period of 6–11 days [16], thus can greatly improve the positive detection rate, especially for the early infected patients pre-HIV seroconversion. Internet-based self-sampling at home plus delivery testing of nucleic acid in laboratory will provide earlier and more accurate testing service for hard-accessible high-risk groups such as MSM. Dried Blood Spots on filter paper (DBS) facilitate preservation of blood total nucleic acids (Total Nucleic Acid, TNA). Characteristics like low biological risk, easy be stored at room temperature within one week with high antibody and nucleic acid stability, make DBSs can be mailed to professional laboratories safely and conveniently [17,18,19]. In this study, we propose a new mode of HIV test and surveillance among MSM population: Internet-based Self-sampling at home plus Laboratory testing of HIV TNA in DBS (ISL of DBS TNA). Feasibility of ISL of DBS TNA was studied. Characteristics of the new mode and that of traditional surveillance mode at HIV voluntary counseling and testing clinic (VCT) were compared.


Procedures of ISL of DBS TNA

A non-governmental organization (NGO) was entrusted by CDC to release information of DBS nucleic acid testing activity on its WeChat Official Account. The entry conditions were: 18 years or older, have sex with men within last year, work or live in Beijing, and participate in the novel surveillance mode for the first time this year. MSM people interested in the activity should filled in the online questionnaire firstly, and then applied for the DBS self-sampling service package online. The NGO would send out the DBS self-sampling service package according to the address and contact information provided in the questionnaire. Participants should read instructions carefully, watch the self-sampling training video online and then complete DBS self-sampling at home. DBSs should be mailed to the designated address after drying. Designated professional laboratory would perform nucleic acid testing of DBSs received and upload test results to a internet platform. Participants could query the nucleic acid test results online with their unique ID. CDC would follow up participants with positive nucleic acid results using IDs and contact information.

Protocol of DBS self-sampling at home

Firstly, stretch your arms downward, close hands and shake them hardly back and forth 5–10 times. Relax your wrist and massage the prick site gently to promote blood circulation. If necessary, hot compress can be applied before finger-prick. Wipe the fingertip with 75% alcohol pads and dry naturally. Unscrew the cap of Unistik 3 18G lancet (Owen Mumford). Compact the platform surface of the lancet on the belly sides of middle or index finger. Press the trigger button to prick and then gently, intermittently, and repeatedly apply pressure to the tissue surrounding the prick site. Drop the fingertip blood in the printing rings on the filter paper to make 3 full blood spots. The blood spot cards should be naturally dried at room temperature for 4 h with no direct sunlight shed on. Put each prepared DBS sample into a Ziplock bag containing a humidity indicator card and desiccant and mail it to a professional laboratory.

The used lancet, hemostatic cotton balls and other consumables contaminated with blood can be sprayed with 75% alcohol, or dried at room temperature for 4 h, then be put back into the ziplock bag for consumables and mailed together with the DBS sample bag to professional laboratories.

The private query card in the service package with a unique query ID number and the query website, and should be kept properly.

Nucleic acid detection method for DBSs

Each whole blood spot was punched off and transferred to a sample tube with a disposable forcep. 1000 μL of DBS pre-extraction reagent (COBAS®AmpliPrep/COBAS®TaqMan®Specimen Pre-Extraction Reagent) was added and incubated in a digital shaking drybath for 10 min with 56 °C at 1000 rpm. Then DBS TNA was extracted using extraction reagent (COBAS®AmpliPrep/COBAS®TaqMan®HIV-1 Qualitative Test, v2.0) on COBAS®AmpliPrep instrument (Roche Diagnostics, Basal, Switzerland) and detected by PCR on a COBAS®TaqMan®48 Analyzer (Roche Diagnostics, Basal, Switzerland). Internal and external controls were set for each run. Positive and negative nucleic acid results were reported as "HIV-1 detected" and "HIV-1 not detected", respectively.

Procedures of surveillance at VCT

The survey subjects was recruited with entry conditions as follows: 18 years or older, have sex with men within last year, work or live in Beijing, and participate in sentinel monitoring for the first time this year. The respondent-driven sampling (RDS) method was adopted to select charismatic members of the target population as seeds for starting the survey, and no more than three cards were issued for each seed to recruit others to participate in the survey. Each card recruited one person. A face to face questionnaire survey was conducted among the subjects after informed consent was obtained by staff with consulting experience and uniform training in accordance with the principles of voluntariness and confidentiality. At the same time, 3-5 mL venous blood samples were collected to detect HIV antibodies by antibody enzyme linked immunosorbent assay (ELISA) with two antibody screening reagents (Wantai, Beijing, China; Lizhu, Shenzhen, China) in the laboratory. If ELISA screening is positive, HIV antibody Western Blot (WB) test will be continued with HIV antibody confirmation reagent (IMT, Shanghai, China).

Statistical analysis

Statistical calculations were performed using SPSS 19.0.


For ISL of DBS TNA mode, a total of 423 questionnaires were completed, and 423 testing service packages were sent out; 340 DBSs were mailed to professional laboratories within one month, of which 323 were qualified samples and 17 samples with insufficient blood collection or without penetrating filter paper are considered unqualified; HIV TNA testing of 323 DBSs were completed, and 7 cases were detected positive. Each corresponding percentage is shown in Fig. 1.

Fig. 1
figure 1


For surveillance at sentinel site, 424 questionnaires were completed and 424 venous blood samples were collected for HIV antibody tested, of which 38 were HIV-1 antibody positive.

Comparison of the composition ratios of the questionnaire population from ISL of DBS TNA and sentinel monitoring at VCT

The questionnaire populations of the two modes were compared using the chi-square test and Fisher's exact test. There was a statistical difference in the overall composition between the population of ISL of DBS TNA and the population monitored at the sentinel sites of VCT (Table 1). Further chi-square analysis showed that ISL of DBS TNA group had a significantly higher proportion of unmarried, company employee/student/official or manager or civil servant, high income group with income > 10,000, sexual orientation of male, no regular sexual partner, previous drug use, HIV testing in the past year, use of post-exposure prophylaxis (PEP), an education level of college or above, and young age (< 40 years old) than sentinel site group (all P values < 0.05).

Table 1 Comparison of the population composition between ISL of DBS TNA and sentinel site at VCT

In the ISL of DBS TNA group, the percentages of workers/commercial services/restaurant and food industry workers, bisexual, sexual role as insertive, previous commercial sex behavious, and history of sexually transmitted diseases (STD) were significantly lower than the sentinel site group (all p-values < 0.05).

Differences in HIV prevalence between the populations from ISL of DBS TNA and sentinel monitoring at VCT

The overall HIV prevalence rate was 2.17% and 8.96% in the populations from ISL of DBS TNA and sentinel monitoring at VCT, respectively (χ2 = 14.953, P = 0.000 < 0.05) and ISL of DBS TNA had a lower HIV prevalence rate than sentinel site in all categories (Table 2). In ISL of DBS TNA, there was an overall difference in infection rates among the occupational categories (Fisher's exact test, P = 0.036), but no significant difference was observed in the pair-wise comparisons; no statistical difference in the overall infection rates was identified in other categories (P > 0.05). In sentinel site, differences in the overall distribution of infection rates by marital status, occupation, sexual orientation, sexual role, condom use for anal sex, history of STDs, HIV testing in the past year, and education level were observed; it was found in the risk assessment that men who were divorced or lost their spouse had a 4.314 times greater risk of infection than those that were married (95% CI 1.561–11.921); the infection risk of commercial service providers was 4.853 times that of company employees (95% confidence interval (CI) 1.645–14.311); the risk of infection for those who were homosexual was 2.783 times greater than the risk of bisexuals (95% CI 1.135–6.823); the risk of sexual role as receptive was 3.136 times greater than the risk of insertive (95% CI 1.412–6.968); the risk of those with a previous history of STD was 4.012 times greater than the risk of those with no previous history of STD (95% CI 1.945–8.276); the risk of those with no previous test for HIV in the past year was 5.235 times greater than that of those having been tested for HIV (95% CI 2.552–10.736). Reasons of lower prevalence in ISL of DBS TNA mode might be composition ratio difference compared with traditional sentinel surveillance, but can also be high acceptability rates of the novel mode, which need to be investigated further in the future study.

Table 2 Differences in prevalence rates between the two surveillance modes

ISL of DBS targets the total nucleic acid of HIV which is detectable 6 to 11 days after exposure, with a much shorter detection window than the one to three months for HIV antibody testing. In ISL of DBS TNA surveillance, 7 samples were found to be positive for nucleic acid, and the nucleic acid testing of the samples were also positive upon repeated testing. The Ct values for the nucleic acid tests were all less than 34, which could be used to determine that the subjects were infected with HIV. Among them, three subjects were exposed 7–10 days prior to testing and six subjects were exposed within seven days to one month. The infected individuals were aged 19–30 years, and all had college education level or above, and one subject was a student. Excluding one blank answer, none of the other 6 infected had received PEP. Five subjects never used condoms during oral sex, and three of them used condoms every time during anal sex, suggesting the necessity of condom use during oral sex in order to prevent infection. Sexually transmitted infections (STIs) were a cofactor of HIV infection [20], and one infected subject had been diagnosed with STD.

Distribution of unqualified DBSs

A total of 340 DBSs were returned, of which 17 were unqualified samples and 323 were correctly collected samples, with a sample qualification rate as high as 95.0%, which confirmed the high success rate and satisfactory results of self-administered blood collection following the instructions in the service package we designed and the operational video we made. To further improve the correct sample rate, the distribution of unqualified samples was analyzed (Table 3), and no statistical difference was found between the categories (all P values > 0.05).

Table 3 Distribution of unqualified DBSs


The purpose of comparing HIV prevalence rates is to reveal the difference in the true infection rates between the two modes. Roche qualitative diagnosis kits with 100% sensitivity and 99.9% specificity were used in our study to detect HIV TNA in DBS specimens, with its’ ellipse period much shorter than window period of antibody detection, thus can provide better performance than antibody test to represent true infection rate. In ISL of DBS TNA mode, assuming HIV TNA positivity representing true infection rate is reasonable. However, as a retrospective study, no paired sentinel DBS samples were collected in the year of 2020, so the corresponding TNA test could not be carried out for sentinel surveillance. In order to prevent undetected infector in sentinel surveillance mode, telephone follow-up and national epidemic database queries were conducted for negative antibody cases with high-risk behaviors. No undetected infectors were found in sentinel surveillance in 2020.

Although HIV prevalence comparison in our study here is not perfect, it can be inferred that the conclusion of lower HIV prevalence in mode of ISL of DBS TNA will not change even when paired sentinel DBSs were collected and TNA tested in the year of 2020. The reason is that TNA test will discover more infections before seroconversion when antibody test failed to find out and thus widen the prevalence gap between ISL of DBS TNA and sentinel surveillance mode. Of course, situation might change if more high-risk population adopted ISL of DBS TNA mode rather than traditional sentinel surveillance at VCT clinic in the future. In the future study, we’ll prepare paired DBSs in sentinel surveilance mode and uncover the true infection rate by HIV TNA.

Compared with the sentinel sites, the population covered by ISL of DBS TNA included more young people with higher education level, higher income, higher proportion of previous use of PEP, higher proportion of HIV testing in the past year, and lower proportion of STDs, indicating that the population using this mode was better educated and more self-protective. However, the high proportion of people without regular sexual partners and the high proportion of previous drug use also suggested high risk of infection in this population. There was an overall difference in the infection by occupation in ISL of DBS TNA (Fisher's exact test, P = 0.036), but no significant difference was observed in pair-wise comparisons. This may be due to the low infection rate in ISL of DBS TNA. The surveillance of ISL of DBS TNA should be scaled up to identify the risk factors in those who use this mode.

The 17 unqualified samples were followed up by telephone calls, and in all cases, the lack of success was due to the subjects’ failure to follow the instructions and the sampling training video. This was the case especially in relation to the lack of attention to the pre-sampling steps of warming and massage, which had resulted in insufficient bleeding volume and a sample that could not be analysed. The blood was not able to transmit HIV four hours after drying, and the return rate of contaminated alcohol pads and lancet was 97.1% (330/340), indicating that the possibility of biological contamination of the environment caused by self-administered blood collection was very low. Therefore, when users read the instructions of the service package and watch the sampling training video online, qualification rate of the self-collected samples in the high-risk population was satisfactory and biohazards were low, making DBS self-sampling feasible to be widely promoted in future HIV surveillance testing.

ISL of DBS mode showed that 6.50% (21/323) of the subjects had received PEP for HIV. Among the 21 individuals, 95.2% were aged 21–39 years, and 100% had college education level or above. All of these subjects tested negative for HIV nucleic acid, which preliminarily suggested the effectiveness of PEP in preventing HIV infections [21].

In ISL of DBS mode, 61.9% of the subjects had used novel drugs such as Rush and the proportion of subjects who had received PEP was higher than the sentinel sites, suggesting that such population was more willing to accept new information, which was an important reason for the acceptance of the self-collected blood samples and delivery for testing in this population.


In conclusion, this study provided a method of DBS preparation through self-sampling among high-risk populations with high qualified sample rate and low environmental biohazard. The method consisted of online questionnaire and offline mailing of the service package. The surveillance route of self-sampling and delivery testing had better confidentiality and covered a population that was a supplement to the sentinel sites. This enabled earlier detection of infected individuals and could be widely promoted in the field of AIDS prevention and treatment.

Further studies are still needed.

Availability of data and materials

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.



Men who have sex with men


Dried blood spot


Total nucleic acid


Internet-based self-sampling at home plus laboratory testing


Voluntary counseling and testing clinic


Non-governmental organization


Center for Disease Control and Prevention


Respondent-driven sampling


Enzyme linked immunosorbent assay


Western Blot


Post-exposure prophylaxis


Sexually transmitted diseases


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This study was supported by the National HIV/HCV Reference Laboratory, National Center for AIDS/STD Control and Prevention, China CDC. Thanks are due to Jingjie Zhao of Guangxi Academy of Medical Sciences for assistance with manuscript revision.


The work was supported by National Science and Technology Major Project of China During the 13th Five-year Plan Period (2017ZX10201101); Beijing Science and Technology Major Project (D171100006717002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Xueli Su, Dongyan Xia, Yanming Sun and Guowu Liu performed analysis of the questionaire data. Xueli Su and Yinxiao Hao designed DBS self-sampling service package. Xueli Su and Yinxiao Hao performed HIV total nucleic acid detection. Xueli Su and Dongyan Xia composed and revised the manuscript. Chun Huang and Hongyan Lu designed the study.

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Correspondence to Xueli Su or Chun Huang.

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The study was approved by Ethical Committee of Beijing Center for Disease Control and Prevention (No. 2018D4). All participants signed the electronic informed consent form in this study. Legally Authorized Representatives of illiterate participants provided informed consent for the study. All methods were carried out in accordance with relevant guidelines and regulations.

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Su, X., Xia, D., Sun, Y. et al. A novel internet sampling for HIV surveillance: feasibility of self-sampling and preparation of DBS for delivery detection of HIV total nucleic acid and complementarity to sentinel surveillance. BMC Infect Dis 23, 509 (2023).

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