Prevalence of Mollicutes among men who have sex with men and transgender women aged 15 to 19 years in Salvador, North-eastern Brazil
BMC Infectious Diseases volume 23, Article number: 244 (2023)
Some species of Mollicutes have been associated with different pathologies of the urogenital tract in humans, with a high prevalence among adult men who have sex with men (MSM) and transgender women (TGW). However, few studies have been performed to investigate its prevalence among adolescents. In this study, we estimated the initial prevalence of Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Ureaplasma urealyticum (UU), and Ureaplasma parvum (UP); the rate of misdiagnosis at different anatomical sites; and the associated factors with positive tests for Mollicutes among MSM and TGW aged 15 to 19 years enrolled in the PrEP1519 study.
PrEP-1519 is the first study to investigate the effectiveness of pre-exposure prophylaxis for human immunodeficiency virus among adolescent MSM and TGW aged 15 to 19 in Latin America. Oral, anal, and urethral swabs were taken from 246 adolescents upon enrolment in the study to detect MG, MH, UU, and UP by quantitative polymerase chain reaction (qPCR). Bivariate and multivariate analyses were conducted by Poisson regression and 95% confidence intervals (95% CI) were estimated.
The prevalence of Mollicutes was 32.1%. UU was the most prevalent species (20.7%), followed by MH (13.4%), MG (5.7%), and UP (3.2%); 67.3% of the positive samples would have been missed if only urethral samples had been taken. Receptive anal sex (prevalence ratio [PR] = 1.79; 95% CI = 1.07–3.01) and clinical suspicion of sexually transmitted infection (PR = 1.62; 95% CI = 1.01–2.61) were factors associated with the detection of Mollicutes in general. Group sex (PR = 1.98; 95% CI = 1.12–3.50) and receptive anal sex (PR = 2.36; 95% CI = 0.95–5.86) were associated with the detection of Mycoplasma spp. No sociodemographic, clinical, or behavioural variable was significantly associated with the detection of Ureaplasma spp.
A high prevalence of Mollicutes was observed among adolescent MSM and TGW, especially at extragenital sites. Further research is required to understand the epidemiological profile of high-risk adolescents in different regions and contexts, and to investigate the pathogenesis of Mollicutes in the oral and anal mucosa before routine screening can be recommended in clinical practice.
Sexually active adolescents constitute a population group that is vulnerable to sexually transmitted infections (STIs) due to several factors of psychosocial and biological nature [1,2,3,4,5]. In addition, adolescents are less likely to be screened for STIs due to anonymity and confidentiality concerns . For some population groups, such as men who have sex with men (MSM) and transgender women (TGW), access to health services is even more challenging, as they are often stigmatized and discriminated in the community and by health workers [7,8,9,10,11], forming barriers to the prevention, treatment, and control of STIs in this population. Furthermore, these barriers are associated with higher rates of human immunodeficiency virus (HIV) infection and other STIs among these populations, regardless of age, thereby also including adolescents and young individuals [12,13,14]. In addition, MSM tend to have more sexual partners, concurrent partners, and older partners [15,16,17], along with unprotected anal intercourse [18, 19]. In relation to TGW, a significant proportion of TGW engage in commercial sexual relationships exposing them to work in precarious conditions and experience sexual violence, often rendering them unable to negotiate the use of condoms with their clients, and thereby increasing the risk of HIV and other STIs . Screening these populations is therefore imperative for the diagnosis and treatment of STIs and the promotion of more adequate prevention and care [21, 22].
Data on the prevalence of STIs and associated factors, along with the risk of acquiring STIs, among adolescent MSM (AMSM) and TGW (ATGW) are extremely limited. Regarding the prevalence of Mollicutes species, the gap in data availability is even greater, since they rarely cause a symptomatic infection, which is one of the reasons why they are neglected in epidemiological surveillance and research. Despite this, this species has been associated with several pathologies of the urogenital tract, such as non-chlamydial non-gonococcal urethritis [23,24,25], male infertility [26,27,28], an increased risk of HIV transmission [29, 30], and antibiotic resistance . This species is highly prevalent in MSM over the age of 18 [32,33,34,35,36], even in extragenital sites (i.e., oral and anal) [37, 38], and is hard to identify in clinical practice due to the absence of symptoms, making them important extragenital reservoirs [37,38,39,40,41]. A missed diagnosis then leads to the persistence of these microorganisms and their potential transmittance to sexual partners [39,40,41,42]. Furthermore, the presence of other common STIs (i.e., chlamydia and gonorrhoea) at extragenital sites of AMSM and ATGW  suggests that Mollicutes may be present at different anatomical sites. The aim of this study was to estimate the prevalence of Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Ureaplasma urealyticum (UU), and Ureaplasma parvum (UP) at different anatomical sites to assess the rate of missed detection and to analyse factors associated with the prevalence of Mollicutes among AMSM and ATGW.
Study design and population
A cross-sectional study based on baseline data obtained from the PrEP1519 cohort study was conducted in three Brazilian cities – Salvador, Belo Horizonte, and São Paulo – which aimed to analyse the effectiveness of HIV pre-exposure prophylaxis (PrEP) among adolescents from key populations who had a history of risk exposure to or vulnerability to HIV infection (e.g. condomless anal sex in the previous 6 months, a history of STI or use of post-exposure prophylaxis (PEP) to HIV in the previous 12 months, commercial sex work). Recruitment of participants was conducted by demand creation strategies on youth social venues and virtual networks (YouTube, Instagram, TikTok, Whatsapp, Facebook and Twitter), including dating apps (Grindr, Tinder and Badoo). Also, public health services and non-governmental organizations (NGOs) could refer eligible participants to the study.
In the present study, baseline data from April 2019 to February 2021 from the city of Salvador, the capital of the North-eastern Brazilian state of Bahia, was analysed. The inclusion criteria were AMSM and ATGW aged 15–19 years old (y/o), who had at least one act of sexual intercourse with another cisgender man or TGW in the past 12 months and had spent most of their time at the study site (i.e., living, studying, working, or residing in one of the study sites). Participants of the PrEP1519 study were offered PrEP and other combination prevention strategies, sexual health care, and STI testing, including those for bacterial STIs, with quarterly follow-ups conducted for up to 3 years.
After checking the eligibility criteria and the information on the proposed steps for the study, individuals who agreed to participate in the study provided written informed consent or assent and were tested at the initial visit (baseline). Cotton swab samples from oral, urethral, and anal sites for MG, MH, UU, and UP were collected to perform real-time polymerase chain reaction (PCR) tests. A socio-behavioural questionnaire was provided at baseline. This study was approved by the Research Ethics Committee of the World Health Organization (Protocol ID: Fiotec-PrEP Adolescent study) and the Federal University of Bahia (# 3,224,384).
A socio-behavioural questionnaire with questions about gender identity, access to health services, sexual practices, drug and alcohol use, and situations of violence was administered at baseline by trained investigators and was used for data analysis. The sociodemographic variables included: age (< 18 or ≥ 18 y/o), race/colour (non-black [white, brown, yellow, other] or black), schooling (elementary school/adult education or high school/higher education), population group (MSM or TGW), and sexual orientation (homosexual/gay/lesbian or bisexual/heterosexual). Behavioural variables were steady sexual partner in the last 3 months (yes or no), casual sexual partner in the last 3 months (yes or no), receptive anal sex (yes or no), insertive anal sex (yes or no), group sex (yes or no), interference of drugs or alcohol in condom use (yes or never), and condom use in the last 3 months (consistent or inconsistent). The clinical variable was clinical suspicion of STI (normal test result or altered test result), which consisted of signs and symptoms suggestive of STI, such as urethral discharge, presence of warts and lesions, dysuria, itching, and irritation, among others.
Collection and storage of biological samples
Oropharyngeal, anorectal, and urethral cotton swabs were taken during the consultation with a doctor or nurse upon enrolment in the study. For the oropharyngeal swabs, the tongue was pressed down using a sterile tongue depressor to avoid contact with the tongue, cheeks, palate, and uvula, and the hydrophilic swab was then rubbed on the tonsils and behind the uvula. For the anorectal sample, the participant was asked to lie on their side with one leg slightly flexed. The swab was then introduced one to two centimetres beyond the rectal sphincter and rotated. For the urethral sample, the foreskin was retracted, exposing the glans, and a paediatric swab was carefully inserted about two centimetres into the urethral meatus and rotated. All samples were stored in 5 mL transport medium at 4ºC until processing . In the laboratory, the samples were homogenized by vortexing (30 s), divided into 1 mL aliquots in microtubes, and were then stored in the refrigerator (-20 °C) until they were used.
Extraction of DNA
The DNA extracted from the samples was obtained using a boiling method with phosphate-buffered saline solution . After extraction, the DNA was quantified and analysed for the presence of contaminants (lipids and proteins) using a NanoDrop™ 2000 spectrophotometer (Thermo Scientific, Brazil) (OD 260/280 and 260/230 ratio).
Conventional and real-time PCR
PCR tests were performed to screen for samples with Mollicutes using primers to amplify a fragment with 280 bp , verified by agarose gel electrophoresis and UV photodocumentation. The positive or indeterminate samples were then submitted to a real-time quantitative PCR (qPCR) assay, performed in a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Brazil), using a TaqMan™ probe and TaqMan™ Universal PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) to amplify the target genes of MG, MH , UU, and UP . The microbial DNA of each microorganism was obtained from the Mycoplasma Laboratory of the University of São Paulo (Brazil) and used as a positive control. Milli-Q water was used as a negative control for detecting contaminants. Positivity was determined when the signal from the sample crossed the threshold, as calculated by the equipment based upon the cycle threshold (Ct) of the positive control.
The bacterial load of the positive samples was estimated from the cycle threshold value using the StepOne™ system (Applied Biosystems, Brazil). To assess whether there was a difference in the load of the different species and at different anatomical sites, the comparisons made were determined by individual variance or error variance (s2), by the Shapiro–Wilks normality test, and the Mann–Whitney U test, because the data distribution was not normal.
To estimate the missed detection of Mollicutes when a particular anatomical site was not screened, the following calculation was performed (see equation below), and Fisher’s exact test was used to calculate statistical significance (p ≤ 0.05):
The sociodemographic and behavioural variables were taken as independent variables for the bivariate analysis. The following dependent (outcome) variables were considered: presence (or not) of each species (MG, MH, UU, UP) of the genera (Mycoplasma spp. and Ureaplasma spp.) or the class (Mollicutes) analysed individually. The bivariate analyses were conducted using Poisson regression with robust variance, estimating the prevalence ratio (PR) and respective 95% confidence interval (CI). The differences between the variables and the occurrence of the outcome were tested using Pearson’s chi-squared test or Fisher’s exact test (when at least one of the expected values was under 5), with significance set at 5% (p ≤ 0.05). For the multivariate analysis, backward stepwise regression was used. The adequate model was selected according to the Akaike information criterion and Bayesian information and was then assessed using the chi-squared test. Stata 15.0 (Stata Corporation, College Station, USA) was used for these analyses.
A total of 246 adolescents were enrolled in the study, most of whom were 18 years or above (85.4%; median age, 18.83 years [18.20–19.44]), were high school or university students (89.3%), were self-identified as black (85.8%), MSM (93.9%), homosexual (64.2%), and had inconsistent condom use (60.7%) (Table 1). Regarding collection refusal, 36 and 33 participants refused the collection of anal and urethral samples, respectively. One oral sample was lost due to mishandling during transportation to the laboratory.
The overall prevalence of Mollicutes, i.e., the proportion of participants who tested positive for any of the four species investigated at any swab site (oral, anal, urethral) was 32.1% (95% CI: 26.5–38.2). UU was the most prevalent species (20.7%, 95% CI: 16.1–26.3), followed by MH (13.4%, 95% CI: 9.7–18.3), MG (5.7%, 95% CI: 3.4–9.4), and UP (3.2%, 95% CI: 1.6–6.4). The two swab sites with the highest prevalence of infection were oral (19.6%) and anal (19.5%), followed by urethral (14.1%).
A high rate of missed detection at the extragenital sites (i.e., oral and anal) was observed when the prevalence per site was compared with the prevalence at all three sites (Table 2). A significant statistical difference was observed in the detection of UU, with a missed detection of 56.5%, 51.7%, and 66.2% if only the oral, anal, and urethral samples were analysed, respectively. For MH, the missed detection rate was 51.5%, 57.5%, and 68.7% if only the oral, anal, and urethral samples were analysed, respectively. Significant statistically missed detection was also observed for MG for the analysis of samples from the urethra (75.4%), as described in Table 2.
Supplementary Figure S1 shows the Ct value of the positive samples for each species of Mollicutes at each swab site. Statistically significant differences were found for MG (p = 0.0008) and UU (p = 0.0087), which had lower Ct values at the anal and urethral sites, respectively, meaning that there was a greater bacterial load of MG at the anal site and a greater bacterial load of UU at the urethral site, thus, a greater risk of infection.
Of the total confirmed Mollicutes infections, 69.6% (55/79) were mono-infections, with 10.9% (6/55) of infections by the same microorganism at more than one anatomical site. As for others, 31.6% (25/79) were co-infections (simultaneous infections. with two or more microorganisms), of which UU and MH were the most prevalent (Table 3).
In the bivariate analysis, having had receptive anal sex (PR = 2.62, 95% CI = 1.07–6.42) and having had group sex (PR = 2.53, 95% CI = 1.42–4.51) were associated with the detection of Mycoplasma spp. None of the variables investigated were significantly related to the detection of Ureaplasma spp. Meanwhile, the only factor that was positively associated with the detection of Mollicutes was receptive anal sex (PR = 1.74, 95% CI = 1.02–2.95) (Table 4). As for the species, only group sex was associated with detecting MH (PR = 2.56, 95% CI = 1.29–5.09). The other variables were not associated with any particular species or had very large confidence intervals, for the analyses of UP, compromising the validity of the results (Supplementary Table 1).
In the final multivariate analysis model, factors associated with positivity of Mollicutes were receptive anal sex (PR = 1.78, 95% CI = 1.03–3.09) and clinical suspicion of STI (PR = 1.46, 95% CI = 1.01–2.49). Having had group sex (PR = 1.97, 95% CI = 1.08–3.59) was associated with testing positive for Mycoplasma spp. No sociodemographic, behavioural, or clinical variable was significantly associated with Ureaplasma spp. As for the species, having had group sex (PR = 2.19, 95% CI = 1.09–4.38) was associated with the detection of MH. No variable was statistically significantly associated with testing positive for MG and UU. As for UP, its low prevalence in our study population (3.2%) indicated that the confidence intervals of the variables were very large, compromising the validity of the results (Table 5).
This is the first study to analyse the prevalence of Mollicutes at three anatomical sites in 15 to 19-year-old MSM and TGW in a Brazilian city. The two most prevalent species in the study population were UU and MH, with a prevalence of 20.7% and 13.4%, respectively, which can be considered high. Although population group, age group, and samples collected differed, a study by Park et al. (2017)  that analysed urine samples obtained from 12 to 19-year-old heterosexual adolescents also found a high prevalence of these species (27.4% for UU and 17.3% for MH). Another study that included MSM aged 18 and over found a high prevalence (around 24.3%) of both species in the samples from anal swabs .
UU is possibly an etiological agent of non-gonococcal urethritis [49, 50], with a prevalence of 5% to 26% in acute urethritis , and is also associated with male infertility [26,27,28]. MH presents intracellular parasitic behaviour in gametic cells, altering semen parameters such as sperm count, motility, and morphology, with a higher prevalence found in infertile (14.5%) than fertile (3.6%) men, which suggests it could also play a role in male infertility . Even so, screening for these species and for UP in asymptomatic, and even symptomatic, individuals are not yet recommended since most of the patients carrying them do not develop the disease. Antimicrobial treatment for eradicating this microorganism is complex and is not clearly associated with a cure; it could even select or induce microbial resistance in mycoplasmas and more severe STIs .
The prevalence of MG found in our study was 5.7% and we considered this to be high. It is similar to the prevalence of 5.1% and 6.6% found by Reinton et al. (2013)  and Soni et al. (2010) , respectively, in anal and oral swabs and urine samples. In a study by Park et al. (2017) , the prevalence in the urine samples taken from heterosexual adolescents aged 12 to 19 was 4.2% and was also low as compared to UU and MH.
Of all the Mollicutes species studied, MG is regarded as an emerging STI in many countries, especially in Europe, where it contributes to 10% to 35% of non-chlamydial non-gonococcal urethritis in men . Further, there is evidence that MG is an important etiological agent of proctitis in MSM  and that inflammation may facilitate the transmission and acquisition of HIV [33, 42]. In this context, screening and adequate treatment for anorectal MG infections is also a strategy for the prevention of HIV, especially for individuals with high-risk sexual behaviours, such as anal sex without the use of a condom . As such, unlike other species, guidelines for its screening and treatment have already been established [21, 56], advising men with recurring non-gonococcal urethritis and women with recurring cervicitis to get tested for MG, preferably with simultaneous genotypic resistance testing to enable the best choice of antibiotic therapy , since some strains of MG have presented clear evidence of antimicrobial resistance to macrolides (like azithromycin) and resistance to quinolones (like moxifloxacin) [31, 58,59,60,61].
One point worth highlighting is treatment failure, which not only induces resistance but can also contribute to the persistence of this pathogen and its transmission to sexual partners during sex without the use of condoms . Therefore, screening for MG and prescribing treatment should be weighed carefully in clinical practice. In addition, increased screening in the population would lead to increased treatment with moxifloxacin or a similar agent instead of azithromycin . A major issue is that moxifloxacin is expensive, difficult to obtain in many parts of the world (it is not available in Brazil through the public health system ) and is already showing resistance to some strains . The risks and benefits of screening and, above all, treating infections caused by MG and especially Mollicutes spp. should be carefully considered.
As for the distribution of Mollicutes between the anatomical sites, there was a higher prevalence of oral and anal infections than urethral infections, which may be a reason why the missed detection rate would be high if only the urethral site had been screened (67.3%). Our findings corroborate those of other studies, which have found 53–85% of chlamydial infections, 64–77.9% of gonococcal infections, and 71.4% of MG infections in extragenital sites, meaning they would not be detected and treated if only urethral screening were performed [37, 63, 64]. These results underline the importance of screening for extragenital STIs, which are often neglected due to the lack of symptoms in most of such infections, thereby implying a failure to detect and treat them, along with their increased prevalence and transmission to other sites in sexual partners [5, 41, 65, 66].
Regarding the occurrence of co-infections, 31.6% of all the participants who tested positive for some species of Mollicutes had two or more simultaneous infections, 92% of which involved UU. A study with MSM who attended a genitourinary clinic observed that UU and UP only ever appeared in co-infections with Gardnerella vaginalis or other Mollicutes . In our study, MH also had a high prevalence in co-infections (8.5%, 21/246) and appeared more in conjunction with other bacteria than in mono-infections (4.9%, 12/246). A similar result was also found by Amorim et al. (2019)  in an investigation of the prevalence of STIs in people treated at specialized STI/HIV clinics in Salvador (Brazil), where MH was more prevalent as a co-infection than a mono-infection.
Species of Mollicutes can also co-occur with other microorganisms, such as gonorrhoea and chlamydia. Therefore, just as screening for Mollicutes is not recommended, screening for it only when the individual tests negative for other STIs may also be a severe problem. For instance, if patients are undiagnosed for MG, and treated for other diagnosed STIs, they may therefore be exposed to antibiotics that are resistant to or induce resistance in Mollicutes during such treatment [62, 68, 69]. Thus, more research is needed to assess Mollicutes co-infection with other microorganisms and the clinical significance of these infections, including pathogenicity at extragenital sites. More assertive screening and treatment guidelines can be developed, reducing the risks of unnecessary routine screening and inappropriate treatment.
Our results indicate a strong tendency for species of Mollicutes to be sexually transmitted, since variables of sexual behaviour, such as group sex and receptive anal sex, were associated with infection by Mollicutes, Mycoplasma spp., and MH. Group sex has already been identified as a risk factor for the acquisition of STIs , as has receptive anal sex without the use of condoms, which is also considered a risk factor for HIV, since the mucosa in this region has many blood vessels that are easily ruptured during intercourse, thereby increasing the risk of transmission of the virus .
The association of these behaviours with the detection of Mollicutes may indicate their sexual transmission. MG has been considered an important agent of STIs for over ten years now [72,73,74]. The transmission of MH follows the same patterns observed in other sexually transmitted organisms, suggesting that it also occurs during condomless sex . Our results, therefore, suggest that species of Mycoplasma and Ureaplasma that colonize the urogenital tract may be possible agents of STIs, which is also confirmed by the high bacterial load of MG in the anal swabs and UU in the urethral swabs taken for this study, indicating that these microorganisms may not just colonize these anatomical sites, but may also evolve to infections inducing symptoms. A study by Bissessor et al. (2016)  corroborates our hypothesis since the bacterial load of MG in the anal samples was higher in the MSM with proctitis than in those with asymptomatic infections (60,000 versus 10,744 copies of the organism).
As for the other variables, clinical suspicion of STI – namely, the presence of signs and/or symptoms of an STI – was also identified as a factor for the detection of Mollicutes. However, other microorganisms that were not investigated could be present and causing the symptoms; therefore, our results cannot be taken as evidence that the symptoms were (or were not) caused by Mollicutes. Thus, we emphasize the need for studies on the pathogenicity of these Mollicutes species in the urogenital tract, orally, and anorectally to determine to what extent and when they can be considered commensal, and when they should be regarded as etiological agents of STIs.
Given the small sample size of ATGW in our study, the statistical analysis for this subgroup could not be properly conducted. However, according to the literature, identifying as a TGW is also associated with STI acquisition . Several studies show that the stigma and discrimination faced by this population make it more challenging for them to access health services [10, 20, 77,78,79,80], including specialized HIV/STI testing and prevention clinics. These barriers end up increasing the prevalence and incidence of STIs in this population . In addition, trans people also face obstacles when entering the formal job market [82,83,84], which is again due to the stigma and discrimination they face, meaning that they often end up as sex workers as their options to make a living are very limited. To complicate matters, they often offer their services in unsafe conditions, making them vulnerable to anal sex without the use of condoms [20, 85] and sexual abuse . Specifically, accessible counselling and prevention strategies are greatly needed for this population. In addition, fighting stigma and discrimination and implementing public policies that guarantee adolescent and young TGW access to education and jobs are essential to prevent such situations of vulnerability and risk.
Our study has some limitations. First of which is that our study population was based on convenience sampling. Our results may not represent the real prevalence of Mollicutes in the total population of adolescent MSM and TGW from Salvador, Brazil, let alone elsewhere, and particularly not in the case of TGW, given the very low sample size of this population. Also, given the overall low sample size of the study, the detection capacity of the statistical tests was limited and many results were not statistically significant. Furthermore, as the study was cross-sectional, it could not be ascertained whether any of the infections detected, symptomatic or not, were new or persistent. Also, we did not perform genotypic antibiotic resistance testing, limiting the selection of an adequate treatment strategy. Finally, the bacterial load was not quantified, limiting our ability to differentiate colonization from infection (especially in the Ureaplasma spp.).
A high prevalence of these microorganisms was found, especially in extragenital sites, and a significant association existed between sexual behaviour and detection of Mollicutes. Therefore, we stress the need to raise awareness in this population regarding the importance of taking preventive measures against the transmission of Mollicutes during sexual intercourse, including oral sex. Further research on adolescents is required to understand their epidemiological profile in other regions and sociodemographic contexts, investigate the pathogenesis of Mollicutes infections in the oropharynx and rectum, and evaluate the benefits of multisite screening for these microorganisms in high-risk adolescents before implementing it in clinical practice.
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Men who have sex with men
Quantitative polymerase chain reaction
Sexually transmitted infection
Human immunodeficiency virus
Among adolescent men who have sex with men
Among adolescent transgender women
HIV pre-exposure prophylaxis
Polymerase chain reaction
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The authors would like to thank the patients who participated in this study.
PrEP1519 is funded by UNITAID (Process 2017–15-FIOTECPrEP), SVS-MS/CNPq (404055/2018–4), and the Ministry of Health of Brazil. This study also was supported by Brazilian National Council for Scientific and Technological Development (409828/2018–1) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Code 001). The funding agencies played no role in the design nor conclusions of the study.
Ethics approval and consent to participate
The study was designed in accordance with the precepts of Resolution 466/12 of the National Health Council. This study was approved by the Research Ethics Committee of the World Health Organization (Protocol ID: Fiotec-PrEP Adolescent study) and the Federal University of Bahia (# 3,224,384).
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The authors declare no conflict of interest.
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Bivariate analysis of the prevalence of M. genitalium, M. hominis, U. urealyticum, and U. parvum among AMSM (N=231). PrEP1519, Salvador, Brazil, April 2019 to February 2021. Supplementary Table 2. Partial Socio-behavioural Questionnaire applied to participants on this study. Figure S1. Ct value (bacterial load) of samples that were positive for M. genitalium (MG), M. hominis (MH), U. parvum (UP), and U. urealyticum (UU) from oral, anal, and urethral swabs taken from AMSM and ATGW enrolled on the PrEP1519 study in Salvador, Brazil (N=246), April 2019 to February 2021.
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de Jesus Salgado, V., de Abreu Oliveira, C.M.P., da Silva, Á.M.B. et al. Prevalence of Mollicutes among men who have sex with men and transgender women aged 15 to 19 years in Salvador, North-eastern Brazil. BMC Infect Dis 23, 244 (2023). https://doi.org/10.1186/s12879-023-08213-z