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Comprehensive knowledge about HIV/AIDS and associated factors among adolescent girls in Rwanda: a nationwide cross-sectional study



Limited comprehensive knowledge of HIV/AIDS is highlighted as one of the major factors linked to the high prevalence of HIV among adolescents and young girls. Thus, it is crucial to identify factors that facilitate or hinder adolescent girls from having comprehensive knowledge of HIV/AIDS. We, therefore, assessed the prevalence of comprehensive knowledge about HIV/AIDS and associated factors among adolescent girls in Rwanda.


We used secondary data from the Rwanda Demographic and Health Survey (RDHS) 2020 comprising 3258 adolescent girls (aged 15 to 19 years). Comprehensive knowledge was considered if an adolescent girl answered correctly all the six indicators; always using condoms during sex can reduce the risk of getting HIV, having one sexual partner only who has no other partners can reduce the risk of getting HIV, a healthy-looking person can have HIV, can get HIV from mosquito bites, can get HIV by sharing food with persons who have AIDS, and can get HIV by witchcraft or supernatural means. We, then, conducted multivariable logistic regression to explore the associated factors, using SPSS (version 25).


Of the 3258 adolescent girls, 1746 (53.6%, 95%CI: 52.2–55.6) had comprehensive knowledge about HIV/AIDS. Adolescent girls with secondary education (AOR = 1.40, 95% CI: 1.13–3.20), health insurance (AOR = 1.39, 95% CI: 1.12–1.73), a mobile phone (AOR = 1.26, 95% CI: 1.04–1.52), exposure to television (AOR = 1.23, 95% CI: 1.05–1.44), and a history of an HIV test (AOR = 1.26, 95% CI: 1.07–1.49) had higher odds of comprehensive HIV knowledge, compared to their respective counterparts. However, girls residing in Kigali (AOR = 0.65, 95% CI: 0.49–0.87) and Northern (AOR = 0.75, 95% CI: 0.59–0.95) regions, and those of Anglican religion (AOR = 0.82, 95% CI: 0.68–0.99) had less odds of comprehensive knowledge compared to those in Southern region and of the Catholic religion.


To increase the comprehensive understanding of the disease at a young age, the need for expanded access to HIV preventive education through formal educational curriculum, and mass and social media via mobile phones is highlighted. In addition, the continued involvement of key decision-makers and community actors, such as religious leaders, is vital.

Peer Review reports


Globally, Human Immunodeficiency Virus and Acquired Immune Deficiency Syndrome (HIV/AIDS) remains a public health concern and sub-Saharan Africa (SSA) has the greatest burden [1,2,3]. Over 38 million people are infected with HIV worldwide, with women making up more than half (19.2 million) of the infected. Sub-Saharan African women account for 15.9 million of the 19.2 million total [4, 5]. Young women and teenage girls account for 25% of new infections in SSA [4, 5]. Eastern and Southern African women face the highest burden of HIV contributing to three in five new infections, while young women aged 15 to 24 years are almost three times more likely than their male peers to acquire HIV infection [4, 5]. It is estimated that over 700 adolescents acquire HIV daily and over 360,000 are expected to die of HIV/AIDS-related illnesses between 2018 and 2030 [6]. Adolescent HIV/AIDS-related mortality in Africa remains significant and AIDS is among the leading causes of death among African adolescents with SSA having the highest AIDS-related mortality burden [7,8,9].

Rwanda’s adult (15 to 64 years) HIV prevalence is 3% with women facing a higher burden compared to men [5, 10]. Although efforts such as screening blood donations, increased support for diagnostic services, health education, prevention services such as free condoms and scaling-up of antiretroviral therapy (ART) have greatly reduced HIV incidence in Rwanda, there exist strong gender disparities in terms of HIV prevalence and incidence [11,12,13]. The Rwanda Demographic and Health Survey (RDHS) 2020 further reports that 9% of Rwandan women engage in sexual intercourse with someone else other than their partner. Of these women having multiple sexual partners, only 45% reported having used a condom during their last sexual intercourse [14]. Societal attitudes and misperceptions about sexuality education have made access to adolescent-friendly, timely and appropriate health education, including comprehensive HIV knowledge a common challenge in many SSA countries including Rwanda [7, 15, 16]. This further exposes adolescents to other unreliable sources of information [15].

Limited comprehensive knowledge of HIV has been documented as one of the major factors linked to the high prevalence of HIV among adolescents and young women (AGYW) [2, 17]. Furthermore, limited HIV knowledge among HIV-positive adolescents also risks a lack of ART adherence, while among the HIV-negative ones, it may lead to increased stigma towards HIV-infected peers, which stigma may hinder many from testing and adhering to ART [7, 18]. Having adequate comprehensive HIV knowledge has been shown to enhance adolescents’ and young women’s ability to negotiate for safer sex hence helping to reduce the risk of contracting HIV [2, 19].

Although efforts aimed at improving comprehensive HIV knowledge among the young and sexually active population have been prioritized, not much success has been registered [2, 7]. Factors such as early marriages, and patriarchal practices that favour boys over girls in terms of accessing education have predisposed teenage girls and young women in SSA to a higher risk of contracting HIV than their male peers [2, 20]. This is made worse by the fact that sexual and reproductive health (SRH) services in Rwanda seem to have insufficient involvement of adolescents in service provision [21, 22]. Moreover, regional studies have also shown healthcare providers’ HIV knowledge scores to be low, with Rwanda scoring lower than the other countries [23].

Nonetheless, there is a dearth of information regarding comprehensive knowledge about HIV/AIDS among adolescents with most of the available HIV/AIDS studies focusing on prevalence, incidence and ART uptake/adherence [3, 8, 10, 11, 13]. In Rwanda, only two studies have recently assessed HIV knowledge and these focussed on healthcare workers and men [12, 23], with none done among adolescents. This creates uncertainty about whether the previous studies’ findings equally apply to women and adolescent girls in Rwanda who tend to have different risks and dynamics.

We, therefore, examined the prevalence of comprehensive knowledge about HIV/AIDS and associated factors among adolescent girls in Rwanda, using a nationally representative dataset. It is crucial to understand and address HIV knowledge gaps among adolescents, since ensuring comprehensive knowledge of HIV/AIDS at an early age appears to be more beneficial as opposed to later in life when the effects of poor/low knowledge of the disease may be irreversible. Moreover, the results of this study would be vital for designing better and more evidence-based behavior change interventions.


Study sampling and participants

The dataset used was from the 2019-20 Rwanda Demographic Survey (RDHS), which was a cross-sectional study and employed a two-stage sample design. The first stage involved cluster selection consisting of enumeration areas (EAs), while the second stage involved systematic sampling of households in all the selected EAs leading to a total of 13,005 households [14]. In particular, the data used in this analysis were from the household and the woman’s questionnaires.

During this survey (RDHS), the data collection period was from November 2019 to July 2020, which took longer than expected due to the COVID-19 pandemic restrictions [1]. Women aged 15–49 years who were either permanent residents of the selected households or visitors who stayed in the household the night before the survey were eligible to be interviewed. Of the 13,005 households that were selected for the survey, 12,951 were occupied and 12,949 were successfully interviewed giving a response rate of 99.9% [14]. The eligible sample was 14,675 women aged 15–49, but 14,634 women were successfully interviewed leading to a 99.7% response rate [14]. For this analysis, we considered only adolescent girls aged 15–19 years interviewed during the survey, and of the selected households, whose sample was 3,258.


Dependent variables

The study outcome variable was comprehensive knowledge about HIV/AIDS, which was a composite variable scored from six yes/no questions; (1) Always using condoms during sex can reduce the risk of getting HIV, (2) Having only one sexual partner, who has no other partners can reduce risk of getting HIV, (3) A healthy looking person can have HIV, (4) Can get HIV from mosquito bites, (5) Can get HIV by sharing food with a person who has AIDS, and (6) Can get HIV by witchcraft or supernatural means [24, 25]. Comprehensive knowledge was considered if an adolescent girl answered all six questions correctly; that is, (“Yes” for questions 1,2, and 3, and “No” for questions 4,5, and 6).

Explanatory variables

Based on the available literature and data, we included possible determinants of comprehensive knowledge about HIV/AIDS [24,25,26,27,28]. Eighteen (18) variables were considered and of these, two were community-level factors that included; place of residence (categorized as rural and urban), and region of residence (Kigali, South, West, East and North). Four household-level factors included; household size (classified into “less than six” and “six and above”), sex of household head (female and male), wealth index (categorized into five quintiles that ranged from the poorest to the richest quintile), and health insurance (yes and no). Wealth index was calculated by RDHS from information on household asset ownership using Principal Component Analysis [14]. Twelve (12) individual-level factors were also considered in the analysis, including; age (categorized as 15, 16, 17, 18 and 19 years), educational level (no education, primary, secondary and tertiary), working status (working and not working), marital status (married and unmarried), religion (Catholic, Protestant, and others), history of having an STI in last 12 months (yes and no), and exposure to news, radio and television (yes and no), contraceptive use (yes and no), having done an HIV test before (yes and no) and owning a mobile phone (yes and no).

Statistical analysis

In this analysis, we applied the DHS sample weights to account for the unequal probability sampling in different strata and ensure the representativeness of the study results [29, 30]. We used Statistical Package for Social Science’s (SPSS) (version 25.0) complex samples package incorporating the following variables in the analysis plan to account for the multistage sample design inherent in the RDHS dataset: individual sample weight, sample strata for sampling errors/design, and cluster number [24, 29]. Initially, we did descriptive statistics for both dependent and independent variables, where frequencies and proportions/percentages for categorical dependent and independent variables have been presented. We, then, conducted bivariable logistic regression to assess the association of each independent variable (i.e. selected socio-demographic factors) with comprehensive HIV knowledge and crude odds ratio (COR), 95% confidence interval (CI) and p-values are presented. Independent variables found significant at the bivariable level with p-values less than 0.25 were then included in the multivariable model. Moreover, independent variables that were non-significant at the bivariable analysis level but were associated with comprehensive knowledge in previous studies were also included in the multivariable logistic regression model. The final model controlled for all the included variables/factors where we calculated and presented their respective adjusted odds ratios (AOR), 95% confidence intervals (CI) and p-values, at a statistical significance level of 0.05. All selected variables in the model were assessed for multi-collinearity, which was considered present if the variables had a variance inflation factor (VIF) greater than 10 [31]. However, none of the variables had a VIF above 3.


We used a weighted sample of 3258 adolescent girls in this study, of which about half had primary education (50.6%), the majority were not working (61.8%), unmarried (97.7%), had health insurance coverage (82.1%), and owned a mobile phone (74.1%). Moreover, a majority had no history of STI (99.6%), were not using contraceptives (97.1%), and had never done an HIV test before (63.5%). Regarding media use, the majority were not exposed to newspapers (62.9%) and television (50.2%) but had radio exposure (82.7%). In addition, the majority of the respondents were from male-headed households (67.8%) of 6 and more members (55.3%), and resided in rural areas (82.2%), as detailed in Table 1.

Regarding knowledge about HIV/AIDS, the majority individually answered correctly all six knowledge questions (range 77.8–94.9%). However, the prevalence of comprehensive knowledge (those who answered all six questions correctly) was 53.6% with a 95% confidence interval of (52.2-55.6%), as shown in Table 2.

Table 1 Background characteristics of Rwandan adolescents aged 15 to 19 years as per the 2020 RDHS
Table 2 Knowledge about HIV/AIDS, as per the 2020 Rwanda Demographic Health Survey, N = 3258

Factors associated with comprehensive knowledge of HIV/AIDS

Results of bivariable analyses are detailed in Table 3, with significant factors independently associated with comprehensive knowledge highlighted. In the adjusted logistic regression model, the factors with significant association include; educational level, religion, health insurance, exposure to television, having had an HIV test before, owning a mobile phone, and region (Table 3).

Adolescent girls with secondary education (AOR = 1.40, 95% CI: 1.13–3.20) had more odds of having comprehensive knowledge about HIV/AIDS compared to those with no education. Those with health insurance (AOR = 1.39, 95% CI: 1.12–1.73) had more odds of having comprehensive knowledge compared to their fellows with no health insurance, similar to girls with exposure to television (AOR = 1.23, 95% CI: 1.05–1.44) who also had more odds compared to those with no exposure. In addition, adolescent girls who had ever tested for HIV (AOR = 1.26, 95% CI: 1.07–1.49) had more odds of having comprehensive knowledge about HIV/AIDS compared to those who had never tested, and the same applied to those owning a mobile phone (AOR = 1.26, 95% CI: 1.04–1.52) who also had more odds compared to their counterparts with no mobile phones. In contrast, respondents from Kigali (AOR = 0.65, 95% CI: 0.49–0.87) and Northern (AOR = 0.75, 95% CI: 0.59–0.95) region had less odds of having comprehensive knowledge about HIV/AIDS compared to their fellows in the Southern region. The same applied to Protestant girls (AOR = 0.82, 95% CI: 0.68–0.99) who also had less odds of having comprehensive knowledge compared to the Catholic respondents.

Table 3 Factors associated with comprehensive knowledge about HIV/AIDS among adolescent girls, as per 2020 RDHS


This study assessed the comprehensive knowledge among Rwandan adolescent girls. We discovered that, despite the progress made, knowledge remains low but still higher than in many other countries, with nearly half of the respondents (53.6%) correctly answering the six HIV/AIDS-related questions in the RDHS. This is consistent with a study assessing knowledge in various SSA countries, which found that Rwandan women had a greater sense of comprehensive knowledge than women in other countries in the region [32, 33]. The study’s results also indicate that Rwandan adolescent girls are relatively knowledgeable about HIV/AIDS transmission modes. Over 80% of the adolescents were well aware that using condoms consistently and having only one sex partner who has no other partners can minimize the risk of contracting HIV, which may help to reduce the number of young people participating in unsafe sexual behavior. They also understood that HIV cannot be transmitted through mosquito bites or sharing food with infected persons, demonstrating the Rwandan population’s progress in dispelling myths and misconceptions that perpetuate stigma against HIV-positive people. Nonetheless, there is room for improvement to attain higher levels of comprehensive knowledge of the disease among young people. Despite the documented link between HIV knowledge and HIV risk, adolescents and young women face other external issues that also predispose them to a higher risk of HIV, such as intimate and gender-based violence, limited access to sexual and reproductive healthcare, amongst others [2, 20, 21, 34]. HIV/AIDS education and awareness programs should thus consider and incorporate such issues for successful overall HIV prevention and reduction.

The educational level of adolescent girls, religion, health insurance, exposure to media, having had an HIV test before, owning a cellphone, and region were all found to be significantly associated with comprehensive HIV/AIDS knowledge. Education was found to have a positive influence on comprehensive knowledge of HIV/AIDS, with those having secondary-level education being more knowledgeable than those with no education. This is not surprising given similar prior findings in other countries such as Malawi [32], Kenya [35], Uganda [36], Ethiopia [37] and Ghana [38]. This stresses the importance of continuing to advocate for and invest in young girls’ and women’s literacy, which has been connected to sustainable women’s empowerment that is passed down through generations since literate women are more likely to send their children to school [39, 40].

Girls who had been exposed to television were more likely to have comprehensive knowledge than those who had not been exposed, a finding also demonstrated in earlier studies [36, 41]. One possible reason is that media exposure enhances the likelihood of coming across HIV/AIDS-related knowledge. Individuals can learn about health and health-related topics from television in a variety of forms, including music, news stories, dramas, movies, and ads, which enhances the odds of reaching people with a variety of interests. Campaigns utilizing television have been reported to be successful in developing countries [42]. As a result, an enhanced partnership between media companies and HIV/AIDS stakeholders is needed to amplify messages and tailor them toward young women and the overall Rwandan community.

Similarly, our study revealed that mobile phone ownership was linked to having a more thorough HIV/AIDS understanding than the lack of it. Mobile phones have recently become a significant part of people’s daily lives, and they are especially appealing to the youth [43, 44]. It is said to have been gradually overtaking ‘traditional media’ such as television [45]. Studies have previously demonstrated that social media can be a useful tool for health promotion [46, 47]. Thus, in addition to increasing mass media outreach, efforts should also be made to work with telecommunication companies to convey messages and to take advantage of social media platforms to spread HIV/AIDS and other health-related information, possibly in collaboration with public figures and individuals whom young women look up to as role models.

Our findings corroborate those of Ethiopian [48] and Ugandan [36] researchers who reported that adolescent girls who had ever been tested for HIV were also more likely to have comprehensive knowledge of HIV/AIDS than those who had never been tested. Counseling before and after an HIV test is essential because it provides for education, prevention of future transmission in the event of a positive result, and an understanding of the need for prompt medical attention [49]. Therefore, having had a test done indicates a person’s potential exposure to information and explains the higher HIV/AIDS comprehensive knowledge. This evidence could be used to influence decisions about capacity building and keeping counselors and healthcare providers in all HIV testing facilities up to date so that they may in turn convey accurate information.

When compared to their counterparts in the Southern region, adolescent girls living in Kigali were shown to be less likely to have comprehensive knowledge of HIV/AIDS. This is in contrast with prior studies in Burkina Faso [50], Nigeria [51], and even outside of the Sub-Saharan African context [52, 53] that reported rural residents to have less HIV-related knowledge compared to urban residents. More research needs to be conducted to fully grasp this difference in dynamics in the Rwandan context.

In many cultures, religion significantly influences how people interact and make decisions regarding their health [54]. While some religious communities have been actively involved in spreading awareness and encouraging testing [55,56,57], others believe that immoral sexual behavior is to blame for HIV/AIDS, which heightens the stigma attached to the disease [57, 58]. Our research revealed that Catholic respondents were more likely than protestant respondents to have comprehensive knowledge of HIV, indicating some variation in how different Christian faith groups have responded to the disease. Comprehensive awareness of HIV/AIDS within the Rwandan community would greatly benefit from the increased involvement of religious leaders of all denominations as well as teamwork and partnerships.

Adolescent girls with health insurance were also more likely to have comprehensive knowledge compared to those with no health insurance. This could be due to the fact that having access to health insurance increases the likelihood of seeking preventive care and therefore multiplying the chances to receive health information [59].

Study strengths and limitations

Since the RDHS data used in this study is nationally representative, the results can be generalized to other Rwandan female populations between the ages of 15 and 19 years. Moreover, DHS data is also known for generating substantial data, with high response rates, high-quality interviewer training, standardized data collection processes across countries, and consistent content across time, allowing these findings to be compared across populations and over time to assess trends. However, there are some notable limitations in this study. First, since the Demographic and Health Survey is collected simultaneously, it is challenging to establish a causal link between the dependent and independent variables. As a result, our findings are bound to associations. Second, given that the DHS is self-reported, there is a chance that under-reporting invalidates our findings, especially given how sensitive the issue of HIV is to respondents. Moreover, we focussed only on Rwandan adolescents, thus future studies should consider doing a multi-country analysis to validate our findings and have more aggregated and broader insight on the topic. Despite these limitations, our findings contribute significantly to the body of knowledge and represent a valuable resource for policymakers who are implementing HIV prevention intervention programs.


Adopting practices that lower the risk of HIV transmission requires a thorough understanding of HIV/AIDS. About half of the Rwandan adolescent girls had comprehensive knowledge about HIV/AIDS and this was associated with various sociodemographic factors; education level, health insurance, exposure to television, owning a mobile phone, history of HIV test, region and religion. To increase the comprehensive understanding of the disease starting at a young age, access to education on HIV prevention must be expanded through interventions including mainstreaming HIV/AIDS into the formal educational curriculum as well as additional sources like mass and social media via mobile phones. The results also showed the necessity for concerned decision-makers to continue involving other key community actors, such as religious leaders, and to expand access to health insurance.

Data Availability

The data set used is openly available upon permission from the MEASURE DHS website (URL: However, authors are not authorized to share this data set with the public but anyone interested in the data set can seek it with written permission from the MEASURE DHS website (URL:



Enumeration area


Adjusted Odds Ratio


Human Immunodeficiency Virus


Acquired Immune Deficiency Syndrome


Sub-Saharan Africa


Antiretroviral therapy


Sexual and reproductive health


Confidence Interval


Crude Odds Ratio


Demographic Health Survey


Rwanda Demographic Health Survey


Variance inflation factor


Statistical Package for Social Science


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We thank the DHS program for making the data available for this study.


No funding was obtained for this study.

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Q.S., J.K. and G.G. Conceived the idea, drafted the manuscript, performed analysis, interpreted the results and drafted the subsequent versions of the manuscript. D.M. and M.W.M. reviewed the first draft, helped in results interpretation and drafted the subsequent versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Joseph Kawuki.

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High international ethical standards are ensured during MEASURE DHS surveys and the study protocol is performed following the relevant guidelines. The RDHS 2019 survey protocol was reviewed and approved by the Rwanda National Ethics Committee (RNEC) and the ICF Institutional Review Board. Written informed consent was obtained from human participants and written informed consent was also obtained from legally authorized representatives of minor participants.

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Kawuki, J., Gatasi, G., Sserwanja, Q. et al. Comprehensive knowledge about HIV/AIDS and associated factors among adolescent girls in Rwanda: a nationwide cross-sectional study. BMC Infect Dis 23, 382 (2023).

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