Pleural effusion caused by Trichinella spiralis infection: two case reports

Abstract

Background

Trichinosis is a worldwide food-borne zoonotic parasitic disease, which is mainly obtained by ingesting undercooked meat containing infected larvae. The purpose of our article is to introduce and discuss two rare cases of pleural effusion caused by Trichinella spiralis.

Case presentation

Here we described two male patients who presented to the respiratory department of our hospital with a massive unilateral pleural effusion, their serum eosinophils were in the normal range, laboratory serological tests revealed that Trichinella spiralis IgG antibody was positive. After the oral administration of antiparasitic drugs, the pleural effusion of two patients was completely absorbed.

Conclusion

Both patients were diagnosed with Trichinosis complicated with pleural effusion, which is very rare in the clinic and easy to be misdiagnosed because of normal eosinophils.

Background

Trichinellosis is a worldwide food-borne zoonotic parasitic disease caused by the infection of all species of Trichinella spp, which is mainly acquired by eating undercooked meat containing infected larvae [1]. From 1964 to 2011, more than 600 outbreaks of human Trichinosis were recorded in China, which were prevalent in southwest, northeast, and central regions. The outbreaks mainly occurred in Yunnan province, the outbreak time was concentrated in winter and spring, and the majority of people were young adults. The World Organization for Animal Health (OIE) defines this disease as a class B zoonotic disease, and China lists this disease as a class II animal disease, which is required to be detected in pig slaughtering and quarantine. Trichinella spiralis infection can cause fever, eyelid edema, muscle pain, eosinophilia, and other symptoms [2]. Severe Trichinosis is characterized by cardiovascular, pulmonary, and central nervous system involvement [3]. Trichinella spiralis infection causes pleural effusion, which is relatively rare clinically. Because clinicians have little understanding of this disease, it is easy to miss and misdiagnose.

Case report

Case 1

A 75-year-old man was admitted to the hospital on December 20th, 2020, because his left pleural effusion was found by physical examination for half a month. He had no obvious edema around the eyes and face, and no muscle pain. Previous history of hypertension. Admission physical examination: Temperature: 36.8 ℃, Pulse: 78 times/min, Respiratory Rate: 20 times/min, Blood Pressure: 143/67 mmHg, Low breath sounds in the lower left lung, normal auscultation in the right lung. Lung Computed Tomography (CT) in our hospital on December 18th, 2020: Moderate pleural effusion on the left side with partial distension of the left lung; the right lung is scattered in solid nodules (Figs. 1, 2). After the patient was admitted to the hospital, the absolute value of serum eosinophils was 0.30 × 10⁹/L (the normal range is 0.05–0.50 × 10⁹/L), Serum creatine kinase was 48 u/L (the normal range is 38–174 u/L). Other serum laboratory tests were normal. Serum protein was 55.1 g/L (the normal range is 65–85 g/L), and serum lactate dehydrogenase (LDH) was 141 u/L (the normal range is 125–243 u/L). Routine examination of pleural effusion: Color: red; Transparency: turbid; Coagulation: there are clots; Specific gravity: 1.022; Rivalta test: positive; Number of nucleated cells: 3180/ul; Mononuclear cells (included lymphocytes and monocytes): 98.9%; Multiple nuclear cells (included neutrophils and eosinophils): 1.1%. Biochemical examination of pleural effusion: Glucose: 5.52 mmol/L (the normal range is 3.9–6.1 mmol/L); Protein: 36.5 g/L (the normal range is 20–40 g/L); Albumin: 22.2 g/L (the normal range is 20–40 g/L); Chlorine: 111.3 mmol/L (the normal range is 110-130 mmol/L); Pleural LDH: 220 U/L (the normal range is 230–460 u/L); Adenosine deaminase (ADA):9U/L(the normal range is 0–18 u/L). No obvious abnormality was found in urological and cardiac ultrasound. Chest ultrasound suggested massive effusion in the left pleural cavity (the maximum anteroposterior diameter is about 7.6 cm). Positron emission tomography/computed tomography (PET/CT) showed: No signs of muscle involvement; no obvious signs of malignant tumor lesions were found at the detection site.

Fig. 1

CT of the lung: obvious effusion signs were seen in the left thoracic cavity, patchy consolidation shadows were seen in the left lower lobe with incomplete swelling

Fig. 2

CT of the lung: the right lung is scattered in solid nodules

The patient received an anti-infective treatment (cefotaxime sodium and sulbactam) for 1 week and then the pleural effusion had no significant decrease. Cytological report of pleural effusion showed that there were a few lymphocytes and mesothelial cells, and no obvious atypical epithelial cells. On the 11th day of admission, he was given diagnostic anti-Tuberculosis treatment for 25 days (rifampicin 450 mg quaque die (QD) + isoniazid 0.3gQD + moxifloxacin 0.4gQD). Pleural ultrasonography showed no obvious pleural fluid absorption, so he stopped using anti-Tuberculosis drugs. We questioned closely the patient's past history, He complained that he ate undercooked pork sausages sold in a small shop a month and a half ago, and after that, symptoms of diarrhea occurred (about 4 days). At this time, we considered whether there was parasitic infection. Therefore, the patient's serum was sent to Wuhan Tongji hospital, and the detection of parasite antibody IgG indicated Trichinella spiralis (+) by enzyme-linked immunosorbent assay (ELISA), and the diagnosis was Trichinosis. Albendazole tablets were given 800 mg/d for 1 week. Two weeks later, a chest ultrasound showed that the pleural effusion disappeared.

Case 2

A 59-year-old male was admitted to the hospital on November 24th, 2021 because of a cough for 1 week. The patient's cough was a mainly dry cough, accompanied by chest wall pain and wheezing at night. The symptoms worsened two days ago, accompanied by white phlegm and hoarseness, but no fever, so he came to our hospital for further treatment. Past physical fitness. Physical examination on admission: Temperature: 36.5 ℃, Pulse: 113 times/min, Respiratory Rate: 19 times/min, Blood Pressure: 119/79 mmHg, SpO₂: 98%. A little moist rale can be heard in the upper right lung, and the left lung was normal, without wheezing or bronchospasm on lung auscultation. After the patient was admitted to the hospital, the absolute value of serum eosinophils was 0.2 × 10⁹/L (the normal range is 0.05–0.5 × 10⁹/L), other serum laboratory tests were normal. Electrocardiogram and purified Tuberculin pure protein derivative (PPD) tests were normal. Serum protein was 61.3 g/L. Routine examination of pleural effusion: color: light yellow; Transparency: slightly turbid; Coagulation: there are clots; Specific gravity: small quantity; Rivalta test: positive; Number of nucleated cells: 2488/ul; Mononuclear cells (included lymphocytes and monocytes): 86.7%; Multiple nuclear cells (included neutrophils and eosinophils): 13.3%. Biochemical examination of pleural effusion: Glucose: 7.38 mmol/L; Protein: 43.4 g/L; Albumin: 28.0 g/L; Chlorine: 108.9 mmol/L; Pleural LDH: 636 U/L; ADA:2U/L. Cytological examination of pleural effusion showed that a large number of inflammatory cells (mainly lymphocytes and neutrophils) and a few tissue cells were observed under a microscope. CT of the lung: Inflammation of the right lower lobe of the lung; Right pleural effusion with right lower lobe atelectasis (Fig. 3). Chest ultrasound suggested a massive pleural effusion on the right side (maximum anteroposterior diameter is about 10.7 cm). On the 8th day after admission, the patient's serum was sent to Wuhan Tongji hospital, and the detection of parasite antibody IgG indicated Trichinella spiralis (+) by ELISA, and the diagnosis was Trichinosis. After oral treatment with mebendazole tablets 300 mg/d for 1 week, his cough symptoms were completely relieved, one month later, the reexamination of lung CT showed that the right pleural effusion was absorbed (Fig. 4).

Fig. 3

CT of the lung: patchy shadow can be seen in the lower lobe of the right lung, and effusion signs can be seen in the right chest cavity with hypodynamia of the lower lobe of the right lung

Fig. 4

CT of the lung: after treatment, the right pleural effusion was significantly absorbed and the lung tissue was restretched

Discussion

Trichinella spiralis belongs to nematodes. Trichinella spiralis can infect pigs, dogs, cattle, cats, wolves, weasels, minks, foxes, whales, and others. Human infection with Trichinella is mainly due to eating raw or semi-raw animal meat (pork, wild boar, dog meat, etc.). Trichinosis patients themselves are not contagious, and their typical clinical manifestations after infection are high fever, periorbital and facial edema, muscle pain, subconjunctival hemorrhage, an allergic rash, and crescent or linear hemorrhage under fingers or toenails. Severe patients may have complications such as myocarditis, encephalitis, or pneumonia [4]. According to statistics from the zoonotic disease surveillance conducted by the European Union (MS) in 2017, half of the member states reported zero cases, and these countries have never reported any cases of Trichinosis [5]. In China, trichinellosis is mainly prevalent in the southwest, northeast, and central regions, and the outbreak mainly occurred in Yunnan Province.

The diagnosis of human Trichinellosis is mainly based on clinical symptoms and serological tests, such as ELISA, indirect immunofluorescence assay (IFA), enzyme immunohistochemistry technique (EIH), and Western-Blotting (WB), The International Commission on Trichinosis recommends ELISA, which detects anti-trichinella IgG in patients' serum by excretory secretion (E/S) antigen of Trichinella spiralis larvae. The sensitivity and specificity of total IgG were 93.6% and 94.3%, respectively [6]. The limitations of this method are that it has a high false-negative rate in the early stage of infection (1–3 months) and cannot distinguish between acute and previous infection [7]. Detection of circulating antigens (CAg), such as anti-immunoelectrophoresis, immunoradiometric assay, direct ELISA, and sandwich ELISA, is an effective method to distinguish between acute and previous infections. CAg is an excretory or secretory antigen produced by live insects, which can directly enter the peripheral blood circulation and can be used for early diagnosis and curative effect evaluation of Trichinosis. However, the detection rate of circulating antigens in serum is usually only 30–50% [8]. Therefore, it is not recommended for the diagnosis of Trichinosis. The discovery of Trichinella larvae cysts from muscle biopsy is the gold standard for the diagnosis of Trichinosis, but due to its invasiveness, the positive rate is only about 50%, and early infection cannot be detected, so it is not recommended [9]. The European Center for Disease Control has classified human Trichinosis into the clinical, laboratory, and epidemiological cases, of which laboratory cases refer to Trichinosis larvae found by muscle biopsy or seropositive. In this paper, the patient had a history of eating pork sausage, and the serological test was positive. After being treated with insect repellent (albendazole or mebendazole), pleural effusion was absorbed, so it was diagnosed as Trichinosis. The treatment plan for Trichinosis includes antiparasitic drugs (mebendazole or albendazole) and glucocorticoids [10]. Our patients were not given glucocorticoids during treatment. Albendazole is currently the preferred drug for the treatment of Trichinosis in China, the dosage is 20–30 mg/(kg·d), divided into two oral courses for 5–7 days [10]. Most patients have a good prognosis and recover within 1–2 months.

We summarized some cases of pleural effusion caused by a parasitic infection in Table 1 [11,12,13,14,15,16,17,18,19,20], the eosinophils of patients with parasitic pleurisy can be in the normal range, and the diagnosis of parasitic pleurisy mainly relies on ELISA of antibodies directed, such as Paragonimiasis, Toxocariasis, and Hydatidosis [21, 22]. The pleural effusion in the two patients in this paper was unilateral, and the pleural effusion was a lymphocyte-dominant exudate. The level of ADA was low, and it was difficult to diagnose Tuberculous pleuritis [23]. The tumor, rheumatism immunity, and other related tests were negative, and there was no significant increase in eosinophils in blood and pleural effusion, so we didn't consider parasitic infection when two patients were admitted to the hospital. The patient of case 1, after further questioning the medical history, he complained that he had recently eaten undercooked pork sausages sold in small shops. Considering that Paragonimiasis pleural effusion caused by eating crayfish was found in the Wuhan area. The clinical manifestations and pleural effusion properties of the two patients in this article are very similar. Therefore, we added serum parasite antibody tests. For case 1, it was speculated that the onset stage of the disease was Trichinosis when the patient ate undercooked pork sausage before 1.5 months, and the acute attack showed gastrointestinal symptoms (diarrhea). Then, pleural effusion was found in the physical examination before a month, which was considered as the late stage of Trichinosis and complications of pleural effusion. Studies have proved that respiratory complications are mostly in the late stage of the disease, that is, between the 3rd and 7th week of infection, bacterial pneumonia, pleurisy, and lung infarction may occur [24]. Therefore, the final diagnosis was Trichinosis complicated with pleural effusion in the late stage.

Table 1 Summary of pleural effusion caused by parasite infection

Conclusion

Trichinosis causes unilateral pleural effusion, which is extremely rare in clinical practice and has not been reported yet. The purpose of this paper is to remind clinicians that parasitic diseases should be included in the differential diagnosis of patients with unexplained pleural effusion, even if their serum eosinophils are within the normal range.