Development and evaluation of a novel fast broad-range PCR and sequencing assay (FBR-PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid diagnosis of invasive fungal diseases: multi-year experience in a large Canadian healthcare zone

Table 5 Performance of molecular assay and standard methods for other types of clinical specimens (non-BALs)^a

		FBR-PCR/S Assay
		Positive	Negative	TOTAL
Fungal culture^c	Positive	14	7	21
	Negative	7^a	47	54
	Total	21	54	75
^cBoth methods had sensitivity (66.7%, 14/21), specificity (87.0%, 47/54), PPV (66.7%, 14/21), NPV (87.0%, 47/54) and efficiency 81.3% (61/75)

^aIncludes all non-BAL clinical specimens tested. Molecular assay results were resolved by clinical review and repeat testing. 7 specimens that were FBR-PCR/S (+)/fungal culture (−) were resolved after clinical review to be true positive molecular tests and false negative cultures. See Tables 2 and 3
^bStandard methods: All isolates were recovered from fungal culture. Yeasts were identified by morphology and Vitek MS while molds were identified by morphology and conventional PCR targeted to the ITS1 and ITS2 gene regions

ISSN: 1471-2334