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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Development and evaluation of a novel fast broad-range PCR and sequencing assay (FBR-PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid diagnosis of invasive fungal diseases: multi-year experience in a large Canadian healthcare zone

Fig. 1

Electrophoresis Gel Image of FBR-PCR/S Assaya. aFBR-PCR/S assay gel image: Lane 1: 100 bp ladder; Lane 2: Clinical sample; Lane 3: Positive Extraction Control (PEC) (S. cerevisiae); Lane 4: Negative Extraction Control (NEC). a Top Panel: Fungal Broad-range (FBR) Panfungal PCR targeting ITS and LSU gene regions. FBR PCR shows two bands, ~ 350 bp ITS band and ~ 900 bp band corresponding to combined amplification of ITS (~ 350 bp) and LSU (~ 600 bp) targets. b Lower Panel: human β-globin PCR. Human clinical sample shows a strong ~ 300 bp band, indicating successful DNA extraction

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BMC Infectious Diseases

ISSN: 1471-2334

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