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Disinfection efficiency test for contaminated surgical mask by using Ozone generator



Ozone (O3) is an effective disinfectant agent that leaves no harmful residues. Due to the global health crisis caused by the COVID-19 pandemic, surgical masks are in high demand, with some needing to be reused in certain regions. This study aims to evaluate the effects of O3 for pathogen disinfection on reused surgical masks in various conditions.


O3 generators, a modified PZ 2–4 for Air (2000 mg O3/L) and a modified PZ 7 –2HO for Air (500 mg O3/L), were used together with 1.063 m3 (0.68 × 0.68 × 2.3 m) and 0.456 m3 (0.68 × 0.68 × 1.15 m) acrylic boxes as well as a room-sized 56 m3 (4 × 4 × 3.5 m) box to provide 3 conditions for the disinfection of masks contaminated with enveloped RNA virus (105 FFU/mL), bacteria (103 CFU/mL) and fungi (102 spores/mL).


The virucidal effects were 82.99% and 81.70% after 15 min of treatment with 2000 mg/L O3 at 1.063 m3 and 500 mg/L O3 at 0.456 m3, respectively. The viral killing effect was increased over time and reached more than 95% after 2 h of incubation in both conditions. By using 2000 mg/L O3 in a 1.063 m3 box, the growth of bacteria and fungi was found to be completely inhibited on surgical masks after 30 min and 2 h of treatment, respectively. Using a lower-dose O3 generator at 500 mg O3/L in 0.456 m3 provided lower efficiency, although the difference was not significant. Using O3 at 2000 mg O3/L or 500 mg O3/L in a 56 m3 room is efficient for the disinfection of all pathogens on the surface of reused surgical masks.


This study provided the conditions for using O3 (500–2000 mg/L) to reduce pathogens and disinfect contaminated surgical masks, which might be applied to reduce the inappropriate usage of reused surgical masks.

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The current situation amid the novel coronavirus 2019 (COVID-19) pandemic has caused economic recession as well as mental health crises around the world. Citizens, especially health care workers, are at risk of infection. The virus spreads between people through small liquid particles due to coughing, sneezing, speaking, or even breathing. Infected secretions can remain in the air for several hours. The pathogen can survive on various surfaces for even longer periods depending on the type of material [1]. In addition to the coronavirus, bacteria or fungi can also be spread by exposure to air and environmental contaminants, including Staphylococcus aureus and Pseudomonas aeruginosa, which are common bacteria that cause infections in humans. Low immunity may cause infectious diseases in wound areas, surgical wounds, and lung infections [2, 3] from airborne transmission within hospitals or from other sources of contamination. These pathogens may also contaminate medical personnel. In addition, there are strains of fungi that can be transmitted through the air in the form of mycelium, mould, and spores such as Aspergillus spp., leading to hypersensitivities such as allergy and asthma [4, 5]. Masks have been recommended as a potential PPE to address the COVID-19 pandemic outbreak and other airborne pathogens. Reuse of a surgical mask is not recommended but has occurred during the recent high usage demands. Effective methods for the industrial disinfection of face masks include the use of hydrogen peroxide vapour, ultraviolet radiation, moist heat, dry heat, and ozone gas [6]. However, the optimal conditions for the disinfection of surgical masks for reuse are still understudied. Ozone is a molecule made up of 3 oxygen atoms (O3) with an unstable structure that has the ability to undergo oxidation reactions, making it toxic to microorganisms. Ozone is a gas that can spread over an area faster than regular liquid spraying. It undergoes oxidation with organic substances and can disinfect any inorganic substance in water and the air with a stronger sterilization effect on pseudoviruses, indicating that it can achieve coronavirus disinfection [7]. Several studies have shown that ozone can kill viruses on hard-to-reach surfaces, including the fabric structure of face masks, over a period of time [4] and that ozone kills 99% of airborne viruses in a period of 15 min [8]. The downside is that ozone can cause skin damage and respiratory irritation, which means it must be used with caution. However, it is highly unstable and has a short half-life and is thus easy to remove. In summary, ozone is a good candidate for surgical mask disinfection; however, the effectiveness of using ozone for disinfection depends on the concentration and time of treatment. Therefore, this study aims to investigate the efficacy of ozone against viral, bacterial, and fungal contamination on the surface of surgical masks. The results from this study will hopefully improve the understanding of the application of ozone in surgical mask disinfection.


O3 generator system

A modified PZ 2–4 for Air, which produced 2000 mg O3/L, and a modified PZ 7 –2HO for Air, which produced 500 mg O3/L, were used together with acrylic boxes. A box sized 0.68 × 0.68 × 2.3 m (1.063 m3) was made of 5 mm thick acrylic with a connector on each side of the box to be easily used with the modified PZ 2–4 for Air O3 generator and to be opened for decontamination of the O3 after completing the experiment by replacing the O3 with O2, as shown in Fig. 1. A half-size box at 0.456 m3 (0.68 × 0.68 × 1.15 m) capacity was constructed the same way (data not shown) for use with a smaller O3 generator, the modified PZ 7 –2HO for Air. Experimentation was performed immediately after gaseous O3 from the O3 generator was introduced into the box until the O3 metre reached 10 ppt. Disinfection of a contaminated mask in a room was performed in a room-sized 56 m3 (4 × 4 × 3.5 m) chamber at room temperature and humidity.

Fig. 1
figure 1

Acrylic box for connection to the O3 generator. Two pieces of 5 mm thick acrylic of size 0.68 × 1.15 (width × length) and 4 pieces of size 0.68 × 0.68 (width × length) were used to construct the box. Each side of the acrylic was designed to have a 25 × 25 mm connector for connection with the O3 generator and for opening to replace the O3 gas with O2. A manual lock was provided on the door side, and wheels were connected for easy movement

Viral preparation

Dengue virus, which is a representative RNA enveloped virus, was propagated in the C6/36 mosquito cell line in a T75 flask at a multiplicity of infection (MOI) of 0.1 [9]. The inoculated cells were incubated at 28 °C without CO2 for 7 days before removal of the supernatant containing new progeny viruses. Infectious particles in the collected supernatant were tested by the focus-forming assay (FFA) followed by the indirect immunofluorescent assay (IFA).

Virus titration (focus forming assay)

Viral infectivity was evaluated and represented as focus forming units per millilitre (FFU/mL) by the focus forming assay [10]. Briefly, monolayer Vero cells in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA) supplemented with 10% foetal bovine serum (FBS) were prepared in a sterile 96-well plate one day before the experiment and incubated at 37 °C with 5% CO2. The supernatant containing the virus was diluted to 1:107 by DMEM on ice before being introduced to 50 µl of cells. Inoculated cells were incubated for 2 h with shaking every 30 min to allow viral infection. A sticky reagent (2% carboxymethyl cellulose (CMC) in DMEM) was added on top to limit viral spreading. Infected cells were incubated at 37 °C with 5% CO2 for 3 days before fixation and permeabilization by 4% formaldehyde in phosphate-buffered saline (PBS) (Sigma Aldrich, USA) and 0.1% Triton X-100 in PBS (Sigma Aldrich, USA). Fixed cells were primed with a primary antibody specific to the dengue virus followed by a secondary antibody labelled with Alexa488 for visualization under a fluorescence microscope. The number of foci was counted and calculated to determine the number of focus forming units (FFU)/mL [11].

Efficiency of ozone efficiency for viral disinfection on contaminated surgical masks

The number of mask-contaminating pathogens was identified by a standard pathogen counting technique before and after ozone treatment under the various conditions. The variables included the concentration of ozone, container size, and time of exposure. To evaluate the viral disinfection efficiency of ozone under various conditions, the optimal concentration of the virus was prepared for the test. A virus concentration of 105 FFU/mL was prepared on ice, and 100 µl (10,000 FFU) was introduced to a sterile surgical mask sized 1 cm2 before placing it in a sterile petri dish. A dish with a contaminated mask was placed in 3 disinfectant conditions: 0.53 m3 with O3 500 mg/L, 1.6 m3 with O3 2000 mg/L, 56 m3 with O3 500 and 2000 mg/L, and with the cover open before running the machine. Time was counted from immediately after 10 parts per trillion (ppt) were measured by the O3 measurement machine (Prozone, Thailand). The contaminated mask was collected from each disinfectant condition after 0 min, 15 min, 30 min, 1 h and 2 h of O3 treatment at room temperature in August in Thailand. For the decontamination of the mask at room temperature, 4 h of O3 treatment was added. The contaminated mask was submerged in 200 µl of sterile DMEM to transfer the virus into the culture medium. The culture medium was subjected to FFA for comparison to the control virus at the starting point.

Efficiency of ozone for bacterial and fungal disinfection on contaminated surgical masks

To determine the antibacterial and antifungal activity of ozone, gram-positive and gram-negative bacteria, namely, Staphylococcus aureus (S. aureus) ATCC29213, Pseudomonas aeruginosa (P. aeruginosa) ATCC27803, and the fungus Aspergillus spp. were used as representative pathogens. The bacteria were subcultured in nutrient broth (NB) and incubated at 37 °C overnight. Subsequently, the organisms were washed by centrifugation and resuspended in 0.9% sodium chloride (normal saline solution), and the concentration was measured spectrophotometrically at 600 nm. Then, the bacteria were adjusted to the desired concentrations with normal saline solution.

For fungal preparation, Aspergillus spp. was cultured on Sabouraud dextrose agar (SDA) and incubated at 25 °C for 3 days. The mould spores were transferred to 0.1% peptone water by using a needle. Then, the spores were counted with a haemocytometer and adjusted to the required concentration with normal saline solution for the experiment.

The bacterial concentration of 103 colony forming units (CFU)/mL and the Aspergillus spp. concentration of 102 spores/mL were separately dropped onto a sterile 1 cm2 piece of surgical mask and placed in a sterile petri dish. The dishes were placed in a small box (0.53 m3; 500 mg/L) and a large box (1.6 m3; 2000 mg/L), and ozone was released through the channel at the cabinet base into the tank until the ozone density reached 10 ppt. The contaminated masks were collected from each disinfectant condition after 0 min, 15 min, 30 min, 1 h, and 2 h of O3 treatment. The fungus-contaminated masks were placed on the SDA. The bacteria-contaminated masks were cultured in sterile nutrient broth and placed on a Mueller–Hinton agar (MHA) surface. Then, the samples were incubated at 37 °C overnight to check the sterility of the contaminated masks [12, 13].


Viral disinfection

At O3 concentrations of 2000 mg/L in a 1.6 m3 box and 500 mg/L in a 0.53 m3 box, the infectious viral particles were inhibited by 82.99% and 81.70% after 15 min of treatment compared to the non-O3-treated virus control. The virucidal effect increased in a time-dependent manner in both conditions: 87.71% and 86.75% at 30 min, 95.59% and 88.64% at 1 h and 98.11% and 97.16% at 2 h of incubation in 1.6 m3 and 0.53 m3 boxes, respectively (Fig. 2). Compared to the virus control, the killing effect was also increased due to the fragile character of the virus at room temperature. To completely eliminate the virus, 2000 mg/L and 500 mg/L treatment for more than 2 h would be required. Regarding the killing effect of the virus in the room-sized space of 56 m3 with O3 concentrations of 2000 mg/L and 500 mg/L, the amount of the virus was reduced by treatment with O3 from the beginning of treatment (83.98%), and the virucidal effect increased to 89.84%, 92.5%, 93.12% and 94.84% after 15 min, 30 min, 1 h and 2 h of incubation (Fig. 3). The effect of O3 in decontamination depended on the concentration and the treatment time.

Fig. 2
figure 2

Percent virucidal effect of ozone treatment at different times of exposure. The virucidal effects of ozone were determined in a 0.53 m3 box (black bars) and a 1.6 m3 box (light grey bars) after 0 min, 15 min, 30 min, 1 h and 2 h of treatment. The dark grey bars show the percentage (%) death of the virus in a control tube without O3 treatment. The data represent the mean and SD of the ozone killing effect, and the value of each is also shown in the table under the graph

Fig. 3
figure 3

Percent virucidal effect of ozone treatment by using O3 2000 mg/L and 500 mg/L in a 56 m3 room after 0 min, 15 min, 30 min, 1 h and 2 h of treatment. The data represent the mean and SD of the ozone killing effect, and the values are shown in the table under the graph

Bacteria and fungus disinfection in a closed-system ozone incubator

The P. aeruginosa, S. aureus and Aspergillus spp. disinfection capability of ozone was tested in a closed-system ozone incubator. The results showed that ozone treatment in small- and large-box conditions could completely inhibit the growth of 103 CFU/mL P. aeruginosa and S. aureus on the mask after 60 and 30 min of treatment, respectively, as shown in Fig. 4. In addition, Aspergillus spp. at a concentration of 102 spores/mL was eliminated within 120 min. In addition, the results of the chamber sterilization experiment showed that bacterial microorganisms were disinfected within 4 h. However, fungal microorganisms were only partially disinfected (Fig. 5).

Fig. 4
figure 4

Potential of O3 to kill a P. aeruginosa b S. aureus and c Aspergillus spp. at different intervals (0 min, 15 min, 30 min, 1 h, and 2 h) in the small box (0.53 m3) and large box (1.6 m3) compared to the untreated control

Fig. 5
figure 5

Ozone killing action against P. aeruginosa and S. aureus and Aspergillus spp. after 4 h in the room compared to the control (untreated)


Wearing a mask is one of the best practices to avoid COVID-19 spread and infection, as recommended by the World Health Organization (WHO. It could also be used for other pandemic infections. Several methods, such as high temperature, UV, ozone, and hydrogen peroxide, have been applied for the reuse, disinfection, and sterilization of disposable masks to avoid a lack of usage in crises and for safety. Each type of mask may require a different method depending on the material used in construction.

Here, we propose the application of O3 in a certain sized container for the reduction and elimination of bacteria and viruses on surgical mask material. A surgical mask is a widely used tool for medical staff in hospitals as well as ordinary people. However, studies concerning the reuse, disinfection, and sterilization of surgical masks are rare compared to those for N95 or filtering facepiece (FFP) respirators [14].

Our results indicated the effectiveness of low-dose O3 (2000 mg/L: 1.02 ppm and 500 mg/L: 0.26 ppm) in decontaminating surgical masks by reducing the amount and inhibiting the growth of viruses, bacteria, and fungi after 15 min, 30 min, and 2 h of treatment with O3 produced from the modified PZ 2–4, which generates 2000 mg O3/L in a 0.53 m3 box. The results are similar to the findings of previous studies in terms of the efficacy of O3 in killing pathogens on surfaces. Dennis et al. found that gaseous O3 inactivated SARS-CoV-2. They also proposed a practical recommendation to implement a simple O3 disinfection box for FFP respirators with 10–20 ppm O3 for at least 10 min. The literature suggests that ozone attacks capsid proteins in nonenveloped viruses and most readily attacks enveloped viruses [15, 16]. The effectiveness of O3 for killing viruses depends on the relative humidity, temperature, and type of virus, as shown in Dubuis et al. 2020, who reported that a higher effect of low-dose O3 exposure (0.23–1.23 ppm) for the inactivation of norovirus was found at 85% relative humidity (RH) for 40 min norovirus, while 20% RH for 10 min gave the same result for bacteriophages. These results suggested that high RH should be used together with O3 to obtain a powerful disinfectant for airborne viruses, which could be implemented inside hospital rooms that are ventilated naturally. However, this study was performed under temperature and humidity conditions in August in Thailand without measuring the exact temperature and RH, although the average temperature was 28 °C and the average relative humidity was 83.2% according to the August 2020 agrometeorological report by the meteorological department [17].

Gram-negative bacteria and fungi require more time for decontamination. There are many reports of O3 lowering the number of bacteria, viruses, and bacterial spores on the surfaces of materials, including figs, fabrics, and plastics, at a relatively low concentration of 1–25 ppm in an average time of 1–4 h [18, 19]. These results link to this study and the experiment of P. aeruginosa and S. aureus closed-system disinfection in a closed system, which showed that bacteria at a concentration of 103 CFU/mL were eliminated within 30 min, and chamber sterilization was achieved within 4 h. Moreover, this experiment successfully achieved the fungal inactivation of Aspergillus spp. by ozone in a closed-system ozone incubator within 120 min. This can be related to previous studies that showed similar results for fungal inactivation. Wood et al. reported on the inactivation of spores of Bacillus anthracis and Bacillus subtilis on building materials by O3 [20]. O3 can diffuse through the cell membrane, and attacking glycoproteins and glycolipids in the cell membrane results in the rupture of pathogen cells. Moreover, O3 attacks the sulfhydryl groups of certain enzymes, resulting in disruption of normal cellular enzymatic activity and loss of function. Ozone also attacks the purine and pyrimidine bases of nucleic acids, damaging DNA [21, 22]. The advantages of ozone gas are that it reaches shadows and crevices in the process of disinfection, unlike ultraviolet radiation which has a short half-life in an airflow environment. The immediately dangerous to life or health concentration (IDLH) of ozone is 5 ppm for humans. Exposure to 50 ppm for 60 min will probably be fatal to humans [23]. Therefore, a low dose in a closed system should be used to avoid direct contact. However, O3 gas can be exchanged quickly by O2, and the odour of O3 is detectable by many people at low concentrations of 0.1 ppm in air in a home environment with air changes per hour varying between 5 and 8 ACH. Ozone has a half-life as short as 30 min [24], and the reaction proceeds faster at higher temperatures (Earth Science FAQ in the picture). Our experiment used a generator machine that produced 2000 mg/L in a 0.53 m3 box.

This study also supported previous studies showing that treatment with ozone causes very low degradation to fibrous structures or the fit of surgical masks. This is unlike other decontamination procedures, such as UV treatment, which enables reuse a limited number of times because of negative side effects, including deformation of the elastic, the accumulation of humidity, and destruction of the fibrous material. This suggested that O3 treatment could maintain the filtration capacity of a mask for reuse more than 30 times [25].

Only 2 sizes of container and 2 concentrations of O3 were used in this study. The temperature and humidity during the experiment were not fixed, which may affect the disinfectant efficiency of ozone, and the filtration capacity of the surgical mask was not determined.


In conclusion, the results of this study supported the possibility of using O3 as an effective procedure for the decontamination of reused surgical masks at a dose of 2000 mg/L O3 in a 0.53 m3 box for 2 h, which could decontaminate surgical masks for reuse by reducing and eliminating the level of pathogens, including bacteria, viruses, and fungi. Longer exposure times lead to greater viral inactivation. Nevertheless, risks for user safety and health remain. Therefore, ozone should be used and handled properly.

Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.


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This project supported by Innovation and Enterprise Affairs, Khon Kaen University, 2019.

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Experimental design: PT, CV, SP, SS, TB, NM, SP. Analysis and summary of results: PT, NM, TB, and SP. All authors discussed the results and implications and commented on the manuscript at all stages. All authors read and approved the final manuscript.

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Correspondence to Supranee Phanthanawiboon.

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Tippayawat, P., Vongnarkpetch, C., Papalee, S. et al. Disinfection efficiency test for contaminated surgical mask by using Ozone generator. BMC Infect Dis 22, 234 (2022).

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