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Misdiagnosed tuberculosis being corrected as Nocardia farcinica infection by metagenomic sequencing: a case report



Disseminated nocardiosis is liable to be misdiagnosed owing to the non-specific clinical manifestations and laboratory/imaging findings. Metagenomic next-generation sequencing (mNGS) is a culture-independent and rapid method for direct identification of all microorganisms in clinical specimens.

Case presentation

A 72-year-old man was admitted to our hospital on February 20, 2019 with a history of recurrent cough, expectoration, fever, and diarrhea since 1 month, and unconsciousness since 1 week. Contrast-enhanced magnetic resonance imaging of head showed multiple lesions in the bilateral cerebral hemispheres, brainstem, and cerebellar hemispheres. The presumptive diagnosis was disseminated tuberculosis, although all tests for mycobacterium were negative. However, the patient did not benefit from antituberculosis treatment. Repeat MRI showed multiple abnormal signals in the brain and progression of meningeal thickening. Cerebrospinal fluid and bronchoalveolar lavage fluid specimens were subsequently sent for PMSeq metagenomics sequencing; the results indicated Nocardia. farcinica as the predominant pathogen. The anti-TB treatment was stopped and the patient was prescribed sulphamethoxazole in combination with linezolid and meropenem for nocardiosis. He showed gradual neurological improvement and was transferred to Huashan Hospital. He was discharged from the hospital on April 19, 2019, but died of persistent diarrhea on May 26, 2019.


Patients with suspected nocardiosis do not always respond to conventional treatment; therefore, mNGS can facilitate diagnosis and timely treatment decision-making.

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The genus Nocardia includes at least 50 species, approximately half of which are pathogenic to humans or animals [1]. This bacterium invades the human body mainly through the respiratory tract, and occasionally through the skin and digestive tract [2]. Approximately 50% of cases of nocardiosis occur in immunocompromised settings such as in patients with tumors, chronic obstructive pulmonary disease, AIDS, organ transplant recipients, and those on long-term steroid or immunosuppressant therapies [3, 4]. Nocardia asteroid is the most prevalent species which accounts for more than 70% of infections, while Nocardia farcinica is more likely to invade immunocompetent hosts than the other Nocardia species [5,6,7,8,9]. It mainly causes lung and systemic infection [10,11,12], but may even cause brain abscess [5]. Kumar et al. reviewed the case reports of nocardiosis published from 1966 to 2011; of these, 41 cases of brain abscess were caused by Nocardia farcinica [10,11,12,13,14]. In the absence of timely treatment, Nocardia farcinica infection is usually life-threatening. The general mortality rate is 14 to 40%, while the mortality rate associated with disseminated infection may be as high as 100% [15].

Owing to the non-specific clinical manifestations, Nocardia infection is liable to be misdiagnosed as tuberculosis, fungal pulmonary disease, or lung malignancy [16]. The diagnosis of Nocardia infection is based on pathogen culture and biochemical identification [17]. However, it normally takes at least 2–7 days of 37 °C aerobic culture for Nocardia to exhibit slow growth and up to 4–6 weeks to develop into visible colonies. Biochemical identification may take even longer. Therefore, it is typically difficult to isolate and identify Nocardia, especially if the specimen is contaminated with other microorganisms.

Metagenomic next-generation sequencing (mNGS) is a culture-independent method that allows identification of all microorganisms in environmental or clinical samples by high-throughput sequencing and genomics analysis of total DNA [18,19,20]. In addition, mNGS takes only approximately 24 h from sampling, nucleic acid extraction, library sequencing, data processing and reporting compared to conventional methods; therefore, it allows for quick identification of pathogens in case of unexplained diseases. We utilized this platform to successfully identify Nocardia farcinica as the main cause of severe disseminated infection in a elderly man, who was initially misdiagnosed as a case of tuberculosis.

Case presentation

A 72-year-old male developed cough, sputum, coryza, fever with chills (body temperature: 37–38 °C), diarrhea, fatigue, and poor appetite since January 2019. However, the patient did not actively seek treatment for these symptoms. On February 13, 2019, the patient experienced an episode of fainting in the toilet followed by spontaneous recovery and he was admitted to the Yuhang District Traditional Chinese Medicine Hospital. Results of laboratory investigations were as follows: hemoglobin, 60 g/L; serum potassium, 2.32 mmol/L; serum C-reactive protein (CRP), 74 mg/L; serum albumin, 28.3 g/L; serum creatinine, 179.8 μmol/L. On the following night, the patient suddenly became unconscious and was referred to the emergency department of the Second Affiliated Hospital of Zhejiang University. The patient was administered empirical and supportive treatment, including antibiotics (sulperazon), respiratory decongestants, and blood transfusion. On February 20, 2019, the patient was admitted to our hospital with chief complaints of recurrent cough, expectoration, fever, and diarrhea for 1 month, and unconsciousness for 1 week. Throat swab culture and influenza virus nucleic acid screening tests were both negative. Contrast-enhanced magnetic resonance imaging of head showed multiple bilateral lesions in the cerebral hemispheres, brainstem, and cerebellar hemispheres (Fig. 1). Physical examination findings at admission were: body temperature, 38.3 °C; heart rate 103 beats/minute, respiratory rate: 18/min, BP 138/73 mmHg, SpO2 94%. The patient was comatose with Glasgow Coma Scale (GCS) score of 1 + 1 + 1. The main differential diagnosis at admission was disseminated tuberculosis, including tuberculous meningoencephalitis, lymph node tuberculosis, intestinal tuberculosis. The cerebrospinal fluid, bronchoalveolar lavage fluid (BALF), and sputum samples were examined on several occasions. Simultaneous amplification and testing for Mycobacterium tuberculosis (SAT_TB) test [21] and routine TaqMan Real-Time PCR assay were separately used to detect mycobacteria RNA and DNA in the above specimens. However, the results of acid-fast staining, tuberculosis RNA, mycobacterium DNA, and GeneXpert were negative. Bacterial, mycobacterial and fungal cultures of cerebrospinal fluid were also negative. After obtaining the consent of family members, the patient was administered a trial of antituberculosis treatment (isoniazid and rifampicin, 600 mg per day, respectively). The patient also received supportive treatment, including dexamethasone to alleviate the intracranial inflammatory response. However, there was no improvement in the consciousness level (GCS score: 1 + 1 + 1). On February 24, 2019, repeat head MRI showed multiple abnormal signals in the brain and progression of meningeal thickening (Fig. 2). In order to confirm the diagnosis, his cerebrospinal fluid specimen was sent to the Wuhan BGI clinical laboratory for PMSeq metagenomics sequencing (https://en-medical-PMSeqs.html) on February 24, 2019. The qualified libraries were constructed using improved DNA Nanoballs (DNB) technology and sequenced on the BGISEQ-50 platform.

Fig. 1
figure 1

(A and B) Contrast-enhanced magnetic resonance images of head obtained at admission (February 20, 2019). Multiple bilateral lesions are seen in the cerebral hemispheres, brainstem, and cerebellar hemispheres

Fig. 2
figure 2

(A and B) Repeat head MRI (dated: February 24, 2019) showing multiple abnormal signals in the brain and progression of meningeal thickening

Specifically, 0.5–3 mL CSF/BALF sample from the patient was collected according to standard procedures. 0.5 mL sample and 1 g 0.5 mm glass bead were mixed in a 1.5 mL microcentrifuge tube and attached to a horizontal platform on a vortex mixer. After vigorous agitation at 2800–3200 rpm for 30 min, 0.3 mL sample was transferred into a new 1.5 mL microcentrifuge tube and DNA was extracted using the TIANamp Micro DNA Kit (DP316, TIANGEN BIOTECH) according to the manufacturer’s protocol. DNA libraries were constructed through DNA-fragmentation, end-repair, adapter-ligation, and PCR amplification. Agilent 2100 and Qubit was used for quality control of the DNA libraries (200–300 bp, > 2 ng/μL). Quality qualified libraries were sequenced using the BGISEQ-50 platform. High-quality sequencing data were generated by removing low-quality reads, followed by computational subtraction of human host sequences mapped to the human reference genome (hg19) using Burrows-Wheeler Alignment. After removal of low-complexity reads, the remaining data were classified by simultaneously aligning to four Microbial Genome Databases, consisting of bacteria, fungi, viruses and parasites. The classification reference databases were downloaded from the NCBI ( RefSeq contains 4945 whole genome sequences of viral taxa, 6350 bacterial genomes or scaffolds, 1064 fungi related to human infection, and 234 parasites associated with human diseases. The detection of pathogen was reported based on the following criteria: 1) the detected microorganisms were related to human infections, which were confirmed by published clinical reports and records; 2) the sequence number of the detected pathogens in the sample should exceed that in the blank control; 3) the sequence number of pathogenic bacteria (except MTB complex), fungi or viruses, should be > 3; 4) the sequence number of pathogenic parasites should be > 100; 5) MTB complex was considered positively detected with more than 1 sequence. Owing to the difficulty in DNA extraction from MTB complex, these pathogens have lower threshold than the other pathogens.

On February 26, 2019, NGS identified Nocardia farcinica as the predominant pathogen (6775 sequence reads); the other pathogens identified were Nocardia. cyriacigeorgica (7 sequence reads) (Fig. 3) and Mycobacterium tuberculosis complex (1 sequence read). The patient was then administered sulphamethoxazole (SMZ) in combination with linezolid and meropenem for anti-nocardiosis treatment; in addition, the anti-TB treatment was continued. Results of pathogen detection in BALF (March 05, 2019) identified Nocardia. farcinica (499 sequence reads); however, no Mycobacterium tuberculosis complex was identified (Fig. 4). These results suggested that the single sequence read of tuberculosis found in the cerebrospinal fluid was the result of contamination; therefore, the anti-TB treatment was discontinued. Smear staining of BALF showed gram-variable branched bacilli with weakly positive acid-fast staining (Fig. 5), which was consistent with the morphological features of Nocardia.

Fig. 3
figure 3

(A) Results of next-generation sequencing for pathogen detection in cerebrospinal fluid (dated: February 26, 2019): Nocardia. farcinica was identified as the predominant pathogen (6775 sequence reads); other pathogens identified were Nocardia. cyriacigeorgica (7 sequence reads) and Mycobacterium tuberculosis complex (1 sequence read). (B) The reads coverage of Nocardia. farcinica was 13.96%

Fig. 4
figure 4

(A) Results of next-generation sequencing for pathogen detection in bronchoalveolar lavage fluid (dated: March 05, 2019) identified Nocardia. farcinica (499 sequence reads); Mycobacterium tuberculosis complex was not identified. (B) The reads coverage of Nocardia farcinica was 0.66%

Fig. 5
figure 5

Smear staining of bronchoalveolar lavage fluid showing gram-variable branched bacilli (A) with weakly positive acid-fast staining (B). The findings are consistent with the morphological features of nocardia

On March 11, 2019, the patient was transferred to the Huashan Hospital of Fudan University. At that time, the patient’s consciousness and tingling sensations had improved (GCS score: 2 + 1 + 3). The patient was prescribed meropenem (2 g, 8 hourly) in combination with SMZ (2 tablets, 8 hourly) for anti-nocardiosis treatment. He was also administered repeated infusions of red blood cell suspension, platelets, and injections of gamma globulin. The patient showed gradual improvement with recovery of consciousness (sensitive to painful stimuli) and was able to exercise. He was discharged from the hospital on April 19, 2019 and was prescribed 3 L/min oxygen inhalation with a metal cannula.

The patient was intermittently followed up telephonically and via the WeChat application. His family members reported that the patient developed persistent diarrhea on May 26, 2019, and was taken to a local hospital in an unconscious state. His blood pressure was not recordable and he died prior to any active treatment.

Discussion and conclusions

Currently, less than 1% of bacteria can be identified by isolation and culture methods [22]. Microscopic observation allows for morphological identification; however, it has low sensitivity and specificity [23]. Serological diagnosis is prone to cross-reactivity and has poor specificity [24]. Polymerase chain reaction (PCR) method fails to detect unknown and highly variable pathogens [25]. Therefore, in up to 40% cases of gastroenteritis and 60% cases of encephalitis, the causative pathogen cannot be determined [26, 27]. In the 1990s, Handelsman et al. pioneered the concept of metagenome. Subsequently, metagenomics was defined as a research method that entails application of modern sequencing technology for direct study of the microbial community in their natural state without isolation and culture [18,19,20]. In recent years, metagenomics has played an increasingly important role in pathogen detection. In a retrospective study of an acute intestinal hemorrhagic epidemic outbreak in Germany, Loman et al. [28] collected 40 samples of Shiga toxin-producing Escherichia coli (STEC)-positive feces and 5 control fecal samples from patients with STEC-negative diarrhea. The pathogenic strain of the disease, STEC O104: H4, was finally detected in samples of STEC-positive patients by metagenomic sequencing without involving isolation and culture; in addition, the Shiga toxin-producing gene fragments were also detected. In 2014, Fisher et al. [29] collected BALF sample from a patient with acute respiratory distress syndrome (ARDS) in Germany. The extracted DNA was subjected to NGS and the causative pathogen was quickly identified as Chlamydia psittaci (within 50 h). Subsequently, the test results were verified using PCR and the patient recovered after administration of specific antibiotics. Various pathogenic microorganisms such as viruses, bacteria, and fungi can cause meningitis; however, the traditional methods for identification of causative pathogen are time-intensive. Guan et al. reported the application of metagenomics to facilitate the diagnosis of suspected viral meningitis. They detected herpes simplex virus type 1 (HSV-1) after NGS sequencing of cerebrospinal fluid samples of 2 patients. Herpes simplex virus type 2 (HSV-2) and human herpes virus type 3 (HHV-3) were detected in the samples of two other patients; all 3 patients were subsequently confirmed by PCR [30]. Yao et al. reported 3 cases of suspected Listeria meningitis in whom the culture results were negative. Sequencing study of the cerebrospinal fluid identified the Listeria sequence, which was further verified by PCR [31]. The above studies demonstrate that metagenomics can rapidly detect rare pathogen infections, which is essential for the diagnosis and treatment of infectious meningitis.

However, mNGS is not the gold standard but a supplementary method for pathogen screening. Although mNGS can detect more difficult-to-culture (DTC) bacterial pathogens in a single run than conventional methods, its diagnostic ability is still impacted by several factors including sample types, sample processing method, sequencing platforms, and strategies. Thus, it may have a lower detection sensitivity than PCR under some circumstances. In addition, mNGS detection is relatively costly, which may impose a financial burden on the patients and the public health system. Therefore, mNGS should only be used to identify potential pathogens when routine laboratory tests, imaging, and pathological assays cannot provide the basis for effective anti-infective treatment.

In our patient, the clinical manifestations and imaging findings strongly suggested an infection of the central nervous system; however, the results of routine tests for detection of pathogenic microorganism were all negative. In addition, patient did not benefit from anti-tuberculosis treatment, which prompted us to use the metagenomics platform for rapid identification of the causative pathogen. Nocardia does not normally colonize the human body and there is limited scope for laboratory contamination; therefore, detection of Nocardia in clinical samples should be considered indicative of infection [32]. The significant read number and genomic coverage of Nocardia farcinica indicated the diagnosis of nocardiosis. SMZ is still the first-choice drug for empirical treatment of nocardiosis; this is because of its good permeability in most tissues (including the central nervous system) and the high serum concentration achieved after oral administration. However, owing to the development of drug resistance, SMZ-based combination therapy is recommended for patients without pathogen identification and antibiotic sensitivity results, especially those with severe infection [15, 33, 34]. Our patient was prescribed SMZ in combination with linezolid and meropenem. The diagnosis of nocardiosis was also supported by the efficacy of anti-Nocardia treatment.

The reported mortality rate associated with nocardiosis is 14–40%; however, it can be as high as 40–87% in patients with hematogenous spread of infection to the brain [15, 33, 34]. The overall prognosis is related to the general condition of the patient, the timeliness of treatment, the spread of infection, and the degree of drug resistance. Our patient suffered from disseminated nocardiosis and showed resolution of neurological symptoms after correct treatment. However, he died of persistent diarrhea on May 26, 2019. It is not certain whether the persistent diarrhea was caused by Nocardiosis, but the poor outcome is consistent with the high mortality rate associated with Nocardiosis in immunodeficient patients [15].

There were two limitations in the present study. At first, due to the need for timeliness and cost-effectiveness of pathogen detection, the library was sequenced only once. In addition, pathogen-specific PCR tests and Sanger sequencing were not conducted to confirm the presence of the pathogen in the sample. We expect to refine these screen-confirmation processes in future investigation.

Availability of data and materials

The datasets generated and analyzed during the present study are available from the corresponding author on reasonable request.



Metagenomic next-generation sequencing


Magnetic resonance imaging


Bronchoalveolar lavage fluid


C-reactive protein


Glasgow Coma Scale




Polymerase chain reaction


Shiga toxin-producing Escherichia coli


Acute respiratory distress syndrome


Herpes simplex virus type 1


Herpes simplex virus type 2


Human herpes virus type 3


  1. Brown-Elliott BA, Brown JM, Conville PS, Wallace RJ Jr. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev. 2006;19(2):259–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Tan CK, Lai CC, Lin SH, Liao CH, Chou CH, Hsu HL, et al. Clinical and microbiological characteristics of Nocardiosis including those caused by emerging Nocardia species in Taiwan, 1998-2008. Clin Microbiol Infect. 2010;16(7):966–72.

    Article  PubMed  Google Scholar 

  3. SL MG, Whiting SE, Baird R, Currie BJ, Ralph AP, Anstey NM, et al. Nocardiosis in the Tropical Northern Territory of Australia, 1997–2014. Open Forum Infect Dis. 2016;3:ofw208.

    Article  Google Scholar 

  4. McNeil MM, Brown JM. The medically important aerobic actinomycetes: epidemiology and microbiology. Clin Microbiol Rev. 1994;7(3):357–417.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Wang HL, Seo YH, LaSala PR, Tarrand JJ, Han XY. Nocardiosis in 132 patients with cancer: microbiological and clinical analyses. Am J Clin Pathol. 2014;142(4):513–23.

    Article  PubMed  Google Scholar 

  6. Wauters G, Avesani V, Charlier J, Janssens M, Vaneechoutte M, Delmee M. Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory. J Clin Microbiol. 2005;43(6):2624–8.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Poonwan N, Mekha N, Yazawa K, Thunyaharn S, Yamanaka A, Mikami Y. Characterization of clinical isolates of pathogenic Nocardia strains and related actinomycetes in Thailand from 1996 to 2003. Mycopathologia. 2005;159(3):361–8.

    Article  PubMed  Google Scholar 

  8. Scharfen J Jr, Moravkova M, Buncek M, Hobza V, Plisek S, Urbaskova P, et al. Nocardia farcinica as the causative agent of a brain abscess in a patient with interstitial lung disease. Epidemiol Mikrobiol Imunol. 2010;59:13–20.

    PubMed  Google Scholar 

  9. Rafiei N, Peri AM, Righi E, Harris P, Paterson DL. Central nervous system nocardiosis in Queensland: a report of 20 cases and review of the literature. Medicine (Baltimore). 2016;95(46):e5255.

    Article  Google Scholar 

  10. Kumar VA, Augustine D, Panikar D, Nandakumar A, Dinesh KR, Karim S, et al. Nocardia farcinica brain abscess: epidemiology, pathophysiology, and literature review. Surg Infect. 2014;15(5):640–6.

    Article  Google Scholar 

  11. Tamarit M, Poveda P, Baron M, Del Pozo JM. Four cases of nocardial brain abscess. Surg Neurol Int. 2012;3(1):88.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Chow FC, Marson A, Liu C. Successful medical management of a Nocardia farcinica multiloculated pontine abscess. BMJ Case Rep. 2013;2013.

  13. Chaudhari DM, Renjen PN, Sardana R, Butta H. Nocardia Farcinica brain abscess in an immunocompetent old patient: a case report and review of literature. Ann Indian Acad Neurol. 2017;20(4):399–402.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kim S, Lee KL, Lee DM, Jeong JH, Moon SM, Seo YH, et al. Nocardia brain abscess in an immunocompetent patient. Infect Chemother. 2014;46(1):45–9.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Martinez R, Reyes S, Menendez R. Pulmonary nocardiosis: risk factors, clinical features, diagnosis and prognosis. Curr Opin Pulm Med. 2008;14(3):219–27.

    Article  PubMed  Google Scholar 

  16. Tellez I, Franco-Paredes C. A woman with chronic subcutaneous swelling of the right foot associated with sinus tracts discharging yellow grains. PLoS Negl Trop Dis. 2010;4(9):e772.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Couble A, Rodriguez-Nava V, de Montclos MP, Boiron P, Laurent F. Direct detection of Nocardia spp. in clinical samples by a rapid molecular method. J Clin Microbiol. 2005;43(4):1921–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol. 1998;5(10):R245–9.

    Article  CAS  PubMed  Google Scholar 

  19. Chen K, Pachter L. Bioinformatics for whole-genome shotgun sequencing of microbial communities. PLoS Comput Biol. 2005;1(2):106–12.

    Article  CAS  PubMed  Google Scholar 

  20. Miller RR, Montoya V, Gardy JL, Patrick DM, Tang P. Metagenomics for pathogen detection in public health. Genome Med. 2013;5(9):81.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Cui Z, Wang Y, Fang L, Zheng R, Huang X, Liu X, et al. Novel real-time simultaneous amplification and testing method to accurately and rapidly detect Mycobacterium tuberculosis complex. J Clin Microbiol. 2012;50(3):646–50.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Torsvik V, Ovreas L. Microbial diversity and function in soil: from genes to ecosystems. Curr Opin Microbiol. 2002;5(3):240–5.

    Article  CAS  PubMed  Google Scholar 

  23. Roingeard P. Viral detection by electron microscopy: past, present and future. Biol Cell. 2008;100(8):491–501.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Doane FW. Immunoelectron microscopy in diagnostic virology. Ultrastruct Pathol. 1987;11(5-6):681–5.

    Article  CAS  PubMed  Google Scholar 

  25. Rose TM. CODEHOP-mediated PCR - a powerful technique for the identification and characterization of viral genomes. Virol J. 2005;2(1):20.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Ambrose HE, Granerod J, Clewley JP, Davies NW, Keir G, Cunningham R, et al. Diagnostic strategy used to establish etiologies of encephalitis in a prospective cohort of patients in England. J Clin Microbiol. 2011;49(10):3576–83.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Finkbeiner SR, Allred AF, Tarr PI, Klein EJ, Kirkwood CD, Wang D. Metagenomic analysis of human diarrhea: viral detection and discovery. PLoS Pathog. 2008;4(2):e1000011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Loman NJ, Constantinidou C, Christner M, Rohde H, Chan JZ, Quick J, et al. A culture-independent sequence-based metagenomics approach to the investigation of an outbreak of Shiga-toxigenic Escherichia coli O104:H4. JAMA. 2013;309(14):1502–10.

    Article  CAS  PubMed  Google Scholar 

  29. Fischer N, Rohde H, Indenbirken D, Gunther T, Reumann K, Lutgehetmann M, et al. Rapid metagenomic diagnostics for suspected outbreak of severe pneumonia. Emerg Infect Dis. 2014;20(6):1072–5.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Guan H, Shen A, Lv X, Yang X, Ren H, Zhao Y, et al. Detection of virus in CSF from the cases with meningoencephalitis by next-generation sequencing. J Neuro-Oncol. 2016;22:240–5.

    CAS  Google Scholar 

  31. Yao M, Zhou J, Zhu Y, Zhang Y, Lv X, Sun R, et al. Detection of Listeria monocytogenes in CSF from three patients with meningoencephalitis by next-generation sequencing. J Clin Neurol. 2016;12(4):446–51.

    Article  PubMed  PubMed Central  Google Scholar 

  32. Chen YC, Lee CH, Chien CC, Chao TL, Lin WC, Liu JW. Pulmonary nocardiosis in southern Taiwan. J Microbiol Immunol Infect. 2013;46(6):441–7.

    Article  PubMed  Google Scholar 

  33. Workman MR, Philpott-Howard J, Yates M, Beighton D, Casewell MW. Identification and antibiotic susceptibility of Nocardia farcinica and N. nova in the UK. J Med Microbiol. 1998;47(1):85–90.

    Article  CAS  PubMed  Google Scholar 

  34. Wilson JW. Nocardiosis: updates and clinical overview. Mayo Clin Proc. 2012;87(4):403–7.

    Article  PubMed  PubMed Central  Google Scholar 

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This work was supported by “Effects of changes in pulmonary flora on the outcome of pulmonary treatment of non-tuberculous Mycobacterium tuberculosis and its immune response” and “Study on the structure of intestinal microbiota in patients with intestinal tuberculosis” (20191203B127, Min-jie Mao).

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LP, MJM, XHP, JKX and XQH designed/performed most of the investigation, data analysis and wrote the manuscript; JKQ XBJ and CHW contributed to interpretation of the data and analyses. YZ contributed to analysis of metagenomic data. All of the authors have read and approved the manuscript.

Corresponding author

Correspondence to Min-jie Mao.

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This study was approved by the ethics committee of Medical Ethics Committee of Hangzhou Red Cross Hospital. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Pan, L., Pan, Xh., Xu, Jk. et al. Misdiagnosed tuberculosis being corrected as Nocardia farcinica infection by metagenomic sequencing: a case report. BMC Infect Dis 21, 754 (2021).

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