Table 5 Detection limit of the LAMP tests with DNA extracted using QIAGEN, and visualized based on calcein detection system
IS2404 copy numbers | Number positive per test | ||
|---|---|---|---|
BURULI Primers | MU 2404 Primers | BU-LAMP Primers | |
300,000 | 1/1 | 1/1 | 1/1 |
30,000 | 1/1 | 1/1 | ¼ |
3000 | 1/1 | 1/1 | 0/5 |
300 | 2/3 | 2/3 | 0/5 |
30 | 2/4 | 1/4 | 0/5 |
3 | 0/4 | 0/4 | 0/5 |