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Table 5 Detection limit of the LAMP tests with DNA extracted using QIAGEN, and visualized based on calcein detection system

From: Evaluation of different DNA extraction methods and loop-mediated isothermal amplification primers for the detection of Mycobacterium ulcerans in clinical specimens

IS2404 copy numbers Number positive per test
BURULI Primers MU 2404 Primers BU-LAMP Primers
300,000 1/1 1/1 1/1
30,000 1/1 1/1 ¼
3000 1/1 1/1 0/5
300 2/3 2/3 0/5
30 2/4 1/4 0/5
3 0/4 0/4 0/5