Fig. 4

Effect of EGTA-AM treatment on expressions of IRF3, FAM26F, and IFN-β. The mRNA and proteins were extracted from HepAD38 and HepG2/pHBV1.3 (300 ng) cells 3 and 5 days post HBV induction respectively. GADPH was used as an internal control. Upregulation of IRF3, FAM26F, and IFN-β expression was seen in HBV+ (+EGTA) cells treated with 5 μM EGTA for 24 h. Significant differences were seen in both HepAD38 and HepG2/pHBV1.3 cell lines for FAM26F (p = 0.02, 0.01; 0.01, 0.01) and IFN-β (p = 0.01, 0.03) expression respectively. However, the increase in expression of IRF3 (p = 0.12, 0.17) was not found significant. All the experiments were performed in triplicates (±SD) and the significance was calculated by Student’s t-test (*P < 0.05). The X-axis indicates the group without EGTA treatment as HBV+(−EGTA) and the EGTA treated group as HBV+(+EGTA), while the Y-axis shows IRF3, FAM26F, and IFN-β expressions in both the cell lines. NS: non-significant