Fig. 5

Gating of memory markers on conventional T cells and gamma delta T cells. Frequency of Vδ2 cells stained with CD27 and CD45RA mAbs. Peripheral blood cells from 22 HIV patients, 10 pulmonary TB patients and 20 controls were stained. Left panel (a) shows a representative pseudo-color plot of CD27 versus CD45RA staining among Vδ2 T cells and αβ T cells from one HIV patient. Right panel (b) depicts bivariate staining of CD27 and CD45RA among Vδ2 T cell and αβ T cells from one representative healthy control. Notably, as shown in the figure (Fig. 5), thresholds discriminating subsets on the basis of CD27 and CD45RA were defined on αβ T cells and copied to plots expressing these markers on gated Vδ2 T cells