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  • Case report
  • Open Access
  • Open Peer Review

Invasive meningococcal disease due to a non-capsulated Neisseria meningitidis strain in a patient with IgG4-related disease

  • 1,
  • 1Email author,
  • 2,
  • 3,
  • 4,
  • 5 and
  • 1
BMC Infectious DiseasesBMC series – open, inclusive and trusted201818:146

https://doi.org/10.1186/s12879-018-3064-2

  • Received: 12 December 2017
  • Accepted: 26 March 2018
  • Published:
Open Peer Review reports

Abstract

Background

Invasive Meningococcal Disease (IMD) is a rare and critical disease in Japan. Most of these cases are caused by capsulated Neisseria meningitidis strains. Non-capsulated (non-typable) strains are considered relatively low-pathogenic and can colonize in the nasopharynx of healthy children and young adults. As far as could be ascertained, only twelve IMD cases due to non-capsulated strains have been reported in the literature. No clear risk factors could be identified in a literature review (unknown or immunocompetent, seven cases; C6 deficiency, three cases).

Case presentation

We report a Japanese male taxi driver with bacteremia and meningitis due to non-capsulated N. meningitidis. He had a fever and shaking chills. Ceftriaxone was administered, and the patient finally recovered. During the clinical course, relative adrenal insufficiency occurred and was treated with hydrocortisone. A hidden co-morbidity, immunoglobulin G4 (IgG4)-related disease, was revealed in the past surgical history (a resection of bilateral orbital tumors), which included symptoms (swelling lachrymal glands and lymph nodes), elevated IgG4, immunoglobulin E, and hypocomplementemia. He recovered finally and no recurrence was observed.

Conclusions

Our IMD case is the first reported in Japan, where IMD is not considered pandemic. The patient had a history of IgG4-related disease, although we could not establish a clear relationship between the patient’s IMD and co-morbidity. A collection of further clinical cases might establish the risk factors and characteristics of IMD that could be caused by this neglected pathogen, non-capsulated N. meningitidis.

Keywords

  • Invasive meningococcal disease
  • Non-capsulated Neisseria meningitidis
  • IgG4-related disease
  • Hypocomplementemia

Background

Invasive Meningococcal Disease (IMD) is a critical disease caused by Neisseria meningitidis. In Japan, IMD was listed as a notifiable disease in April 2013. Approximately 60 cases were reported to Japanese national surveillance from April 2013 to December 2014 [1].

N. meningitidis causing IMD usually have the capsule [2]. Encapsulated bacteria are resistant to humoral immunity and have a tendency to disseminate hematogenously. On the other hand, non-capsulated (non-typable) N. meningitidis strains are not resistant to opsonization and seldom cause invasive infection in healthy humans. Therefore, a search of the literature reveals only a small number of case reports of IMD due to non-capsulated N. meningitidis.

As far as we could ascertain, this is the first Japanese case of IMD due to non-capsulated N. meningitidis. The patient had an underlying diagnosis of immunoglobulin G4 (IgG4)-related disease. By describing this case and the review of the past literature, we discuss the neglected pathogen, non- capsulated N. meningitidis. And we consider the possible relationship between IMD and IgG4-related disease.

Case presentation

A 51-year-old male taxi driver was admitted to Fukuoka City hospital with high fever (40 °C) and a shaking chill despite his use of over-the-counter antipyretics. He was transferred to the emergency department about 14 h after the symptoms onset. His medical history included bronchial asthma, pneumonia, and a surgical procedure for the resection of bilateral orbital tumors at 40 years of age. The patient had neither a history of contact with sick persons nor of any travel abroad. His body height was 160 cm and his body weight was 52 kg.

At the time of admission, his vital signs were as follows: blood pressure, 108/74 mmHg; heart rate, 90 beats/min; respiratory rate, 40 breaths/min; body temperature, 40.1 °C; and Glasgow Coma Scale, E4V4M6 (total 14/15). Upon physical examination, a swelling of the bilateral eyelids (Fig. 1a) and petechiae were detected on the conjunctiva. Some dental caries, purpura and petechiae were noted on the limbs (Fig. 1b, c), and 1–2 cm sized swollen lymph nodes noted at submandibular and inguinal areas were remarkable. Neck stiffness was not detected. Laboratory data on admission revealed high inflammation (white blood cell 13,500 μL, and c-reactive protein 12.29 mg/dL), normocytic anemia (Hemoglobin 8.7 g/dL), high protein and low albumin levels (total protein 12.3 g/dL and albumin 2 g/dL), renal failure (serum creatinine 4.52 mg/dL), hyperkalemia (6.12 mEq/L), proteinuria and hematuria (Table 1). Computed tomography showed enlargement of the liver and bilateral kidneys (Fig. 2a, b) in addition to swelling of the cervical, supraclavicular, axillary, mediastinal, and celiac lymphadenopathy.
Fig. 1
Fig. 1

A swelling of bilateral eyelids (a) and purpura and petechiae on the limbs (b, c)

Table 1

The laboratory data of the patient

Hematology

Chemistry

Serology

WBC

13,500

/μl

TP

12.3

g/dl

IgG

7392

mg/dl

Neutro

84.5

%

Alb

2

g/dl

IgG4

3200

mg/dl

Lymph

9.6

%

Glu

69

mg/dl

IgA

78

IU/ml

Hgb

8.7

g/dl

T-Bil

0.37

mg/dl

ANA

< × 40

 

HCT

25.9

%

AST

13

IU/l

SS-A

7.8

U/ml

PLT

15.4

104/μl

ALT

26

IU/l

SS-B

7.0

U/ml

Coagulation

LDH

282

IU/l

CH50

<12.0

U/ml

 PT %

40

%

CK

123

IU/l

C3

37

mg/dl

 APTT

48.6

sec

BUN

46.3

mg/dl

C4

7

mg/dl

 FDP

10.5

μg/ml

Cre

4.52

mg/dl

ACTH

66.9

pg/ml

Urinalysis

Na

125

mEq/l

Cortisol

16.7

μg/dl

 Protein

(1+)

 

K

6.12

mEq/l

sIL-2R

5390

U/ml

 Blood

(1+)

 

Cl

102

mEq/l

Procalcitonin

> 10

ng/mL

 Bence-Jones protein

(−)

 

CRP

12.29

mg/dl

   

 β2-microglobrin

103,000

μg/l

      
Fig. 2
Fig. 2

The enlargement of liver and bilateral kidneys (a, b) detected by using computed tomography

Severe infectious disease was suspected, and we started ceftriaxone 1 g intravenously every 12 h after drawing a blood culture. However, purpura spread rapidly and the progression of drowsiness and fever was sustained on the 2nd hospital day. We performed a lumbar puncture and the turbid spinal fluid analysis revealed meningeal inflammation (white blood cell count, 2370 /fields; protein, 293 mg/dL; and glucose, 16 mg/dL), which was compatible with bacterial meningitis (Fig. 3). Gram-staining of the spinal fluid showed gram-negative diplococci. The blood culture also became positive for gram-negative diplococci. We strongly suspected that the septic status of the patient’s meningitis and bacteremia was due to N. meningitidis. We increased the dose of ceftriaxone to 2 g intravenously every 12 h. The patient’s general status and consciousness then improved and his fever subsided. On the 5th hospital day, the patient’s fever (38 °C) re-appeared with hypotension, hyponatremia and eosinophilia, which led to a suspicion of relative adrenal insufficiency due to meningococcemia. We started a daily intravenous administration of 300 mg hydrocortisone on the 7th hospital day, and tapered the regimen to a daily oral administration of hydrocortisone on the 11th hospital day. Ceftriaxone was stopped on the 12th hospital day. No recurrence of infection was observed and he was discharged on the 25th hospital day. The clinical course is summarized in Fig. 4.
Fig. 3
Fig. 3

A lumbar puncture was performed and the turbid spinal fluid was collected

Fig. 4
Fig. 4

Clinical course of the case. The patient was managed successfully with antibiotic and hydrocortisone and discharged on day 25

We investigated the patient’s co-morbidities and performed microbiological analysis of the strain. His past surgical history (bilateral orbital tumors), his symptoms (swelling lachrymal glands and lymph nodes), the elevated levels of IgG4 (3200 mg/dL) and immunoglobulin E (304 IU/ml), and hypocomplementemia (C3 37 mg/dL, C4 7 mg/dL, and CH50 < 12.0 U/ml) were the characteristics of Mikulicz’s disease included in IgG4-related disease. Furthermore, a biopsy of the patient’s lymph nodes confirmed the presence of IgG4-related disease (IgG4/IgG index 100%). We prescribed oral prednisolone 30 mg a day on the 19th hospital day. In addition, the strain of N. meningitidis was identified as non-typable. The sequence type (ST) was identified as ST-11448, which belongs to ST-23 (ST-23 complex), the most common ST in Japan [3]. Moreover, we also analyzed the N. meningitidis strain according to PorA and FetA typing, which has a higher resolution power than that of ST, and identified that the PorA VR1, PorA VR2 and FetA were 5, 2–82, and F4–1, respectively. These results suggested that this N. meningitidis strain was a non-capsulated derivative of the domestic ST-23 strain. (See Discussion and conclusion).

Discussion and conclusion

As far as we could ascertain, this is the first Japanese case among IMD cases due to non-typable N. meningitidis. This is also the first case of N. meningitidis causing IMD in a patient with IgG4-related disease.

The mechanism of N. meningitidis infection relies on three factors to avoid the human immune response system: the formation of a capsule, the production of IgA protease, and molecular mimicry. Among these factors, the formation of a capsule contributes the most to the progression of IMD [4]. A capsule can prohibit opsonophagocytosis in the human body. Twelve serogroups of N. meningitidis (A, B, C, Y, W, X, Z, 29E, H, I, J, L) were divided according to the antigenicity. Among these serogroups, the five (A, B, C, Y, W) make up the majority of IMD causative pathogens, for which vaccines are available [5]. On the other hand, non-typable strains are considered to be of low-pathogenicity [6].

Only 12 IMD cases due to non-typable strains could be found in a literature review by using PubMed and Ichushi (the Japanese database for medical literature and conference proceedings) databases (Table 2) [4, 712]. Although the risk factors of most cases are unknown, three C6 deficiency (hypocomplementemia) cases are described in the literature [7, 12]. Hypocomplementemia is an established risk factor of IMD [13]; however, there has been no report of the relationship between hypocomplementemia and IMD due to non-typable bacteria. Opsonophagocytosis with antibodies and complements is thought to prevent the invasion of non-capsulated bacteria [13].
Table 2

The previous reports of invasive meningococcal disease cases due to non-typable Neisseria meningitidis

Case

Age

Sex

Risk Factor

Result of Culture

Outcome

Hummell DS, et al. 1987 [7]

0

M

C6 deficiency

CSF: positive Blood:negative

Survive

0

M

C6 deficiency

CSF: positive Blood:negative

Survive

Vogel U, et al. 2004 [4]

42

ALL, GVHD

Blood: positive

Survive

Hoang LM, et al. 2005 [9]

13

F

Immunocompetent

CSF: positive, Blood: negative

Death

Findlow H, et al. 2007 [8]

12

M

Unknown

CSF: positive

Survive

13

M

Unknown

CSF: positive

Survive

11

F

Unknown

CSF: positive

Survive

Johswich KO, et al. 2012 [10]

13

M

Unknown

Blood: positive

Unknown

Zheng Xu, et al. 2015 [11]

7

F

Immunocompetent

Blood: positive

Survive

Ganesh K, et al. 2017 [12]

45–64a

M

Diabetes mellitus COPD

Pleural aspirate: positive

Survive

15–24a

M

Unknown

CSF: positive

Survive

5–9a

M

C6 deficiency

Blood: positive CSF: positive

Survive

F female, M male, ALL Acute lymphoblastic leukemia, GVHD graft versus host disease, COPD chronic obstructive pulmonary disease, CSF cerebrospinal fluid

aThe age of three patients in reference [12] were described as age category

The laboratory data in our case revealed hypocomplementemia. At first, we suspected that hypocomplementemia appeared secondary due to sepsis [14]. However, because the patient’s vital signs did not reach the level of shock status [14], hypocomplementemia was considered to be secondary to the patient’s sole medical background, IgG4-related disease. Hypocomplementemia is a characteristic of IgG4-related disease; however, that does not suggest increased susceptibility to infection. Also, the patient had not taken immunosuppressive agents such as corticosteroids. We finally concluded that this was an accidental occurrence of IMD in this patient with IgG4-related disease. There remains the possibility that his job (taxi driver) was associated with the acquisition of the pathogen due to close contact with many persons. In a German study, 1.7% of healthy children and young adults possessed the non-capsulated N. meningitidis in their pharynx [6].

Based on a microbiological viewpoint of N. meningitidis, ST-11448 has a difference of only 1 nucleotide in the fumC gene (G at 207 to C) compared with those in ST-23 strain; however, this strain was uniquely recorded as ST-11448 in the MLST database at the time of Feburuary 2018 [15]. In addition, according to the Neisseria PorA and FetA typing database, [16, 17] 43 meningococcal strains were recorded as PorA VR1: 5 and FetA: F4-1, and among those, only one strain had been isolated in Sweden 2006, belonged to ST-23. On the other hand, a meningococcal strain listed as PorA VR2: 2-82 could not be found in the database. Taking the molecular epidemiological resolution of PorA and FetA typing into consideration, these microbiological results suggested that this N. meningitidis strain was a non-capsulated derivative of a domestic ST-23 strain rather than one imported from outside Japan.

In conclusion, this is the first documented case of IMD with non-typable N. meningitidis in Japan. Since the patient had no immunosuppressive drugs and the underlying disease was not considered to be an immunocompromising condition, we concluded that IMD had developed accidentally. This non-typable strain is considered to be low-pathogenic and few cases have been reported. We intend to collect more clinical cases that will allow us to establish the characteristics of IMD caused by this neglected pathogen.

Abbreviations

IgG4: 

Iimmunoglobulin G4

IMD: 

Invasive Meningococcal Disease

Declarations

Acknowledgements

None.

Funding

The microbiological analysis in this case report was supported by Grants-in-Aid for Scientific Research (KAKENHI) Grant Number 15 K08485.

Availability of data and materials

All data containing relevant information to support the study findings are included in the manuscript.

Authors’ contributions

SK, KO, HY and YN are the attending physicians for the patient and collected medical data of the patient. HT performed microbiological analysis of the clinical sample. SK and KO wrote the manuscript. KY and NS made a critical revision of this manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Consent for publication

Written informed consent was obtained from the patient for publication of his medical and personal details including all accompanying images.

Competing interests

The authors declare that they have no competing interests.

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Authors’ Affiliations

(1)
Division of Infectious Diseases, Fukuoka City Hospital, 13-1 Yoshizuka-Honmachi, Hakata-ku, Fukuoka 812-0046, Japan
(2)
Department of Ophthalmology, Graduate School of Medical Science at Kyushu University, Fukuoka, Japan
(3)
Division of Infection Control and Prevention, Kurume University Hospital, Kurume, Japan
(4)
Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan
(5)
Center for the Study of Global Infection, Kyushu University Hospital, Fukuoka, Japan

References

  1. Trends in invasive meningococcal disease, week 13, 2013 to week 52, 2014, Japan. National Institute of Infectious Diseases. https://www.niid.go.jp/niid/ja/bac-megingitis-m/bac-megingitis-iasrs/5864-pr4271.html. Accessed 5 Nov 2017. (in Japanese).
  2. Jafri RZ, Ali A, Messonnier NE, Tevi-Benissan C, Durrheim D, Eskola J, et al. Global epidemiology of invasive meningococcal disease. Popul Health Metr. 2013;11:17.View ArticlePubMedPubMed CentralGoogle Scholar
  3. Takahashi H, Kuroki T, Watanabe Y, Tanaka H, Inouye H, Yamai S, et al. Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 in Japan by multilocus sequence typing. J Med Microbiol. 2004;53:657–62.View ArticlePubMedGoogle Scholar
  4. Vogel U, Claus H, von Müller L, Bunjes D, Elias J, Frosch M. Bacteremia in an immunocompromised patient caused by a commensal Neisseria meningitidis strain harboring the capsule null locus (cnl). J Clin Microbiol. 2004;42:2898–901.View ArticlePubMedPubMed CentralGoogle Scholar
  5. Caugant DA, Maiden MC. Meningococcal carriage and disease-population biology and evolution. Vaccine. 2009;27(Suppl 2):B64–70.View ArticlePubMedPubMed CentralGoogle Scholar
  6. Claus H, Maiden MC, Maag R, Frosch M, Vogel U. Many carried meningococci lack the genes required for capsule synthesis and transport. Microbiology. 2002;148:1813–9.View ArticlePubMedGoogle Scholar
  7. Hummell DS, Mocca LF, Frasch CE, Winkelstein JA, Jean-Baptiste HJ, Atilio Canas J, et al. Meningitis caused by a nonencapsulated strain of Neisseria meningitidis in twin infants with a C6 deficiency. J Infect Dis. 1987;155:815–8.View ArticlePubMedGoogle Scholar
  8. Findlow H, Vogel U, Mueller JE, Curry A, Njanpop-Lafourcade BM, Claus H, et al. Three cases of invasive meningococcal disease caused by a capsule null locus strain circulating among healthy carriers in Burkina Faso. J Infect Dis. 2007;195(7):1071.View ArticlePubMedGoogle Scholar
  9. Hoang LM, Thomas E, Tyler S, Pollard AJ, Stephens G, Gustafson L, et al. Rapid and fatal meningococcal disease due to a strain of Neisseria meningitidis containing the capsule null locus. Clin Infect Dis. 2005;40:e38–42.View ArticlePubMedGoogle Scholar
  10. Johswich KO, Zhou J, Law DK, St Michael F, McCaw SE, Jamieson FB, et al. Invasive potential of nonencapsulated disease isolates of Neisseria meningitidis. Infect Immun. 2012;80:2346–53.View ArticlePubMedPubMed CentralGoogle Scholar
  11. Xu Z, et al. First case of Neisseria meningitidis capsule null infection in China. Infect Dis (Lond). 2015;47:591–2.View ArticleGoogle Scholar
  12. Ganesh K, Allam M, Wolter N, Bratcher HB, Harrison OB, Lucidarme J, et al. Molecular characterization of invasive capsule null Neisseria meningitidis in South Africa. BMC Microbiol. 2017;17:40.View ArticlePubMedPubMed CentralGoogle Scholar
  13. Lewis LA, Ram S. Meningococcal disease and complement system. Virulence. 2014;5:98–126.View ArticlePubMedGoogle Scholar
  14. Hebert LA, Cosio FG, Neff JC. Diagnostic significance of hypocomplementemia. Kidney Int. 1991;39:811–21.View ArticlePubMedGoogle Scholar
  15. PubMLST. Neisseria Sequence Typing Home Page. https://pubmlst.org/neisseria/. Accessed 23 Feb 2018.
  16. PubMLST. Neisseria PorA Typing. https://pubmlst.org/neisseria/PorA/. Accessed 23 Feb 2018.
  17. PubMLST. Neisseria FetA variable region typing. https://pubmlst.org/neisseria/FetA/. Accessed 23 Feb 2018.

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