Multiplex detection in tonsillar tissue of all known human polyomaviruses

BMC Infectious Diseases

Table 1 Sequences of primers and probes, amplicon sizes, amplicon positions (in VP1 region) in target genome and reference strain used in the 13-plex Luminex assay [59]

Virus	Forward/Reverse (5′ biotinylated) Primers sequence (5′-3′) Probe (5′ amine-C12) sequence (5′-3′)	Amplicon length (bp)	Amplicon position (bp) in target genome	Reference strain (GenBank accession no.)
JCPyV	AATGAGGATCTAACCTGTGGAA/CTGCACCATTGTCATGAGTTGCTTG ATGAATGTGCACTCTAATGG	127	1742–1868	J02226
BKPyV	ACAGAGGTTATTGGAATAACTAG/ACTCCCTGCATTTCCAAGGG CTTAACCTTCATGCAGGGTC	143	1952–2094	DQ305492
KIPyV	TTGGATGAAAATGGCATTGG/TAACCCTTCTTTGTCTAAAATGTAGCC CTTGGAACAGCTAATAGTAGAATC	142	2263–2404	EF127906
WUPyV	TTGGATGAAAATGGCATTGG/TAACCCTTCTTTGTCTAAAATGTAGCC GAGTACATACAGGGCTTTCCAG	142	2411–2552	EF444554
MCPyV	TTCCATCTTTATCTAATTTTGCTT/AGGCCTAGTTTTAGATTACCAGAC GTAATAGGCCCACCATTTGT	144	3757–3900	EU375803
HPyV6	TTGCTTCTGGATCCAATACTGC/GGCCTCAGGAATTTCAGGCAA TGGATGCTGGTTCATCTCTG	131	1426–1556	HM011558
HPyV7	AAGCAGCTACAACTGGGAACTT/GGCCTCAGGAATTTCAGGCAA GCCTACCTTATCCTATGAGTG	125	1450–1574	HM011566
TSPyV	AGAATGTATGATGACAAAGGTAT/TCTGTAGTTTCCAGTTAGAAAC TGAGGGAATGAATTTCCATATGTT	111	1722–1832	GU989205
HPyV9	ATCTATGGCTCATCCTCAGG/GTAGAGCTAGCAACTAGGCCT AGTGCAGGGTACCACTCTC	107	1862–1968	KC831440
HPyV10	GTCCAGTTCCTACTAAAGTTCCT/TACATCATTGCCCATCCTTGGTT GCCGGACACCACAATGACA	106	1501–1628	JQ898292
STPyV	TGAATATGATCCGTGCCAAA/ACTGCATCAGGGCCTACTTG CCTCCTCCAACATGTGTTCC	129	1318–1446	JX463184
HPyV12	GTAATGGCACCCAAGAGGAA/GGGGATTTAGAAAGGCCTCA CCCAGCAGTGTCCCTAAATT	157	1402–1558	JX308829
NJPyV	TGTGTGCCAAAGAAGTGTCCT/TCTGTCACCTGTTGGAGCATT CTGATGCTACTACTGAAATTGAA	159	1113–1271	KF954417

Sequences for primers and probes for all except MCPyV, STPyV, HPyV12 and NJPyV as in Gustafsson et al [53].

ISSN: 1471-2334