Fig. 2

Discrimination of PPNG isolates with the G547A substitution in bla _TEM allele by using T220-f/T220-r primers. Lanes 1 and 2, indicate the 204 bp PCR products from strains IM4519 and IM4520 with the G547A mutation in bla _TEM allele; lanes 3, 4, 5 and 6 indicate PPNG control strains containing bla _TEM-1 and bla _TEM-135, respectively, without amplification; lane M, molecular size marker