 Research article
 Open access
 Published:
Sample size considerations using mathematical models: an example with Chlamydia trachomatis infection and its sequelae pelvic inflammatory disease
BMC Infectious Diseases volumeÂ 15, ArticleÂ number:Â 233 (2015)
Abstract
Background
The success of an intervention to prevent the complications of an infection is influenced by the natural history of the infection. Assumptions about the temporal relationship between infection and the development of sequelae can affect the predicted effect size of an intervention and the sample size calculation. This study investigates how a mathematical model can be used to inform sample size calculations for a randomised controlled trial (RCT) using the example of Chlamydia trachomatis infection and pelvic inflammatory disease (PID).
Methods
We used a compartmental model to imitate the structure of a published RCT. We considered three different processes for the timing of PID development, in relation to the initial C. trachomatis infection: immediate, constant throughout, or at the end of the infectious period. For each process we assumed that, of all women infected, the same fraction would develop PID in the absence of an intervention. We examined two sets of assumptions used to calculate the sample size in a published RCT that investigated the effect of chlamydia screening on PID incidence. We also investigated the influence of the natural history parameters of chlamydia on the required sample size.
Results
The assumed event rates and effect sizes used for the sample size calculation implicitly determined the temporal relationship between chlamydia infection and PID in the model. Even small changes in the assumed PID incidence and relative risk (RR) led to considerable differences in the hypothesised mechanism of PID development. The RR and the sample size needed per group also depend on the natural history parameters of chlamydia.
Conclusions
Mathematical modelling helps to understand the temporal relationship between an infection and its sequelae and can show how uncertainties about natural history parameters affect sample size calculations when planning a RCT.
Background
In the field of infectious diseases, planning the required sample size for an intervention trial raises special issues. The success of an intervention to prevent the disease complications by reducing exposure to infection is influenced by the natural history of the infection. Different assumptions about the temporal relationship between the infection and the development of sequelae can affect the expected effect size of an intervention. To calculate the required sample size for a trial investigating the reduction in complications of an infection, we need to make assumptions about the effect size of the intervention (e.g. the reduction in relative risk) and the incidence of the infection sequelae.
Mathematical models are often used to study the dynamics of infectious disease transmission. Researchers have also suggested that mathematical models could help to improve the design of randomised controlled trials (RCTs) of complex interventions to prevent infectious disease transmission [1â€“4]. In a recent example, a deterministic compartmental model was developed to inform the design and monitoring of the HIV Prevention Trials Network study PopART (HPTN 071). PopART is a threearm clusterrandomized trial to investigate the effectiveness of antiretroviral therapy and other interventions to reduce HIV transmission at the population level [5, 6]. Mathematical models are particularly useful for investigating processes and mechanisms that are difficult to observe in practice [3], e.g. the development of complications of infections that might be diagnosed sometime after exposure to the infection [7]. The effect size of an intervention can be derived within the mathematical modelling framework. The results obtained for the effect size can then be used to determine the required sample size.
Chlamydia trachomatis (chlamydia) infection in the lower genital tract can ascend to cause pelvic inflammatory disease (PID) in women which, in turn can cause ectopic pregnancy and tubal factor infertility. Chlamydia is the most common bacterial sexually transmitted infection in many developed countries [8, 9] and is mostly asymptomatic in women, but treatable with antibiotics if diagnosed [10]. There is still considerable uncertainty about how long after initial infection C. trachomatis ascends to the upper genital tract, resulting in PID, and the fraction of infected women who will develop PID [11â€“13].
There is great interest in interventions that could reduce the risk of chlamydiaassociated PID because this might prevent future tubal factor infertility. The Prevention Of Pelvic Infection (POPI) RCT investigated the effect of offering young women in London a screening test for chlamydia on the incidence of PID one year later [14, 15]. Women were randomly allocated to an intervention group, which received immediate testing and treatment for women with positive chlamydia test results. The control group reflected routine care, but swabs were collected at baseline and stored. Testing and treatment were then deferred for one year. The incidence of PID in the trial was lower in the intervention than the control arm but the confidence intervals included the null effect and the investigators argued that the trial was underpowered [14, 15]. We used published data and assumptions from the POPI trial to: investigate how different assumptions about the temporal relationship between chlamydia infection and the development of PID can influence the sample size calculation; and to investigate how a mathematical model can be used to inform sample size calculations for an RCT.
Methods
Model
We used a SusceptibleInfectedSusceptible (SIS) compartmental model (Fig.Â 1) to imitate the structure of the POPI RCT. We ran the model separately for intervention and control groups, using different starting conditions because chlamydia status at baseline differed in the intervention and in the control group (Additional file 1, section 1) [16]. Women in the intervention group are all initially susceptible (S) because they have been tested for chlamydia and received (presumed) successful treatment if indicated. In the control group, a percentage of women is infected (I), reflecting the chlamydia prevalence in the study population. We used a constant force of infection, assuming that a single screening test in women participating in the RCT did not change the population prevalence of chlamydia, i.e. susceptible women can become infected at a constant rate Î». Infected women can clear the infection naturally at rate r. We investigated differences in the temporal relationship between chlamydia infection and PID, as described previously, by separating the infectious period into two stages [16]. The first stage represents infected women without PID (I_{1}) who can progress at rate Î³ to the second stage where the infected women have developed the complication PID (I_{2}). This results in the following system of ordinary differential equations:
The force of infection Î» is calculated so that the steady state prevalence in the model is equal to the prevalence p of the study population. The duration of infection is assumed to be exponentially distributed with a mean duration of 1/r, i.e. some women will clear the infection rapidly whereas others remain infected for longer time periods [17, 18].
PID incidence and intervention effect for three types of progression
We examined three hypothetical mechanisms for the timing of progression from chlamydia infection to PID [16]: first, PID could develop soon after C. trachomatis infects the lower genital tract (immediate progression); second, PID could develop at any time throughout the course of lower genital tract infection (constant progression); or third, PID could develop just before natural clearance from the lower genital tract (progression at the end). For each type of progression it is assumed that, of all women infected, a certain fraction f will develop PID in the absence of an intervention (Fig.Â 1). If there is immediate progression the PID incidence equals fÎ»S, we set Î³â€‰=â€‰0 and Iâ€‰=â€‰I_{1}â€‰+â€‰I_{2}. If PID develops at a constant rate the PID incidence equals Î³I_{1}; to achieve the same cumulative PID incidence as the other two types of progression we set \( \gamma =\frac{fr}{1f} \). Note, the mean duration of infection is still 1/r (see Additional file 1, section 1). If PID develops at the end of a chlamydia infection PID incidence equals frI setting Î³â€‰=â€‰0 and Iâ€‰=â€‰I_{1}â€‰+â€‰I_{2}.
In the absence of an intervention, the PID incidence is the same for all three types of progression, i.e. the cumulative PID incidence rates in the control group at followup time t are the same. The cumulative PID incidence in the intervention group at followup time t, derived for each type of progression by the model, is used to calculate the corresponding relative risk (RR) of PID (RRâ€‰=â€‰risk of PID in intervention group/risk of PID in control group). The RR is independent of the fraction f for the immediate progression and for the progression at the end in contrast to the RR for the constant progression (Additional file 1, section 2).
Sample size calculations used in the POPI trial
The sample size calculation for the POPI trial was based on a comparison of two proportions. Standard formulae used to calculate the sample size need assumptions about the PID incidence in both groups after the followup period, or about PID incidence in the control group and the RR (Additional file 1, section 3) [19]. The POPI trial investigators published two sample size calculations (TableÂ 1). Before the start of the trial, they assumed a 2Â % PID incidence after one year in the control group and RRâ€‰=â€‰0.48 resulting in a total sample size needed of 4122 (with 80Â % power and a 5Â % significancelevel), if there was no loss to follow up, based on a published RCT. In that RCT, 7Â % of women in the intervention group had a positive chlamydia test result at baseline and the incidence rates of PID one year later were 8 per 10,000 woman months in the intervention and 18 per 10,000 woman months in the control group (RR 0.44, 95Â % CI 0.200.90) [20]. During the POPI trial, the investigators revised their sample size calculation, owing to slow enrolment. The revised calculation cited a cohort study suggesting a higher PID incidence (9.5Â % to 12.0Â % over four years in three different groups of women) [21]. In the revised calculation, they assumed a PID incidence of 3Â % and calculated the sample size needed to detect a RR of 0.44; a sample size of 2274 women would be required to detect this effect size with 80Â % power and a 5Â % significance level [14, 15].
In this study, we first reexamined the data used for the two sample size calculations in the POPI trial:

Scenario 1, 2Â % PID incidence and RRâ€‰=â€‰0.48;

Scenario 2, 3Â % PID incidence and RRâ€‰=â€‰0.44.
We determined the fraction f such that the PID incidence in the control group after the followup period equals the PID incidence assumption using baseline values for all other parameters (TableÂ 1). For each type of progression from chlamydia to PID, we used the model to derive the incidence of PID in intervention and control groups, and the corresponding RR. We used the standard formula for calculating the sample size and compared the results with the original calculation, varying the power of the trial from 10â€“90Â %. A sensitivity analysis for scenario 1 was done by varying duration of infection and prevalence, keeping baseline values for all other parameters.
Generic sample size calculation using the mathematical model
In a second step, we investigated how the mathematical model can inform sample size calculations in general. We used the model and the three different types of progression to examine sample size requirements using different values for the natural history parameters of chlamydia infection instead of assuming a specific PID incidence in the control group.
First, we used baseline values for all parameters (TableÂ 1) to derive the PID incidence in the control group, the RR for each type of progression and the sample size needed per group. We then changed the duration of infection and fraction developing PID using baseline values for all other parameters (TableÂ 1). The analysis was repeated for different values of chlamydia prevalence. We also investigated the change of PID incidence in the control group and the RRs while varying the followup time from three to 18Â months using baseline values for all other parameters (TableÂ 1).
For all analyses, the sample size needed per group was calculated using the chisquare test for the comparison of two proportions [19]. Analytical results were derived in Mathematica 10 and numerical solutions were obtained in R (version 3.1.1) [22, 23]. Code files can be obtained from the authors on request. This study used published data only so it did not require approval by an ethical committee.
Results
Sample size calculations used in the POPI trial
Different sets of published assumptions about PID incidence rates and the size of intervention effect lead to different conclusions about the temporal relationship between chlamydia infection and PID (Fig.Â 2). In scenario 1, the relationship between the power of a trial and the sample size required per group is compatible with the hypothesis that PID can develop throughout the course of infection (Fig.Â 2a). Assuming a constant progression rate from chlamydia to PID results in a RR of 0.49, which is close to the original RR assumption in the POPI trial (RRâ€‰=â€‰0.48). If chlamydia progresses to PID only at the end of the infectious period, the model predicts a RR of 0.39. In this scenario, 28.6Â % of infected women have to develop PID to achieve the 2Â % PID incidence after one year of followup.
In scenario 2, the assumptions used for the second sample size calculation (RRâ€‰=â€‰0.44, 3Â % PID incidence) are more compatible with the hypothesis that PID develops at the end of a chlamydia infection (Fig.Â 2b). This model results in a RR of 0.39, as before. In a model that assumes a constant progression rate from chlamydia to PID, the predicted RRâ€‰=â€‰0.56. In this scenario, 42.9Â % of chlamydiainfected women have to develop PID to achieve an incidence rate of 3Â % after one year of followup. The RR and the sample sizes needed per group are always higher for constant progression to PID than for progression at the end of infection (Fig.Â 2). If chlamydia infection progresses to PID only at the end of infection, infection can resolve naturally before they are at risk of PID, whereas with constant progression the risk of developing PID remains throughout the infectious period.
Under the hypothesis that C. trachomatis results in PID almost immediately after infection, the sample size needed per group is >21,000 for the examined range of power (data not shown) and the predicted PID incidence in the intervention group is higher than in the control group (RRâ€‰=â€‰1.05 in both scenarios). In this situation, testing and treatment are too late to prevent any PID cases. On the contrary, women who are successfully treated in the intervention group are at risk to become newly infected and to develop PID again.
In the sensitivity analysis for scenario 1, the fraction of women who develop PID in order to achieve the 2Â % PID incidence ranged from 15.9 to 80.3Â % at different levels of chlamydia prevalence and duration of infection (Additional file 1: Figure S2). For constant progression and progression at the end of infection, changing duration of infection influences the RR and the sample size needed per group more than changing prevalence (see Additional file 1: Table S1 and Figure S3). The sample size calculated with constant progression is closer to the POPI trial sample size calculation than with progression at the end of infection in 78.5Â % of the investigated combinations (see Additional file 1: Figure S4). The hypothesis of immediate progression was not investigated because the estimated RR was >1 in the main analysis.
Generic sample size calculation using the mathematical model
Assuming a chlamydia prevalence of 7Â %, a mean infection duration of one year, and that 10Â % of infected women will develop PID, we expect a PID incidence of 0.007 after one year of followup. TableÂ 2 shows the resulting RR and the sample size needed per group for each type of progression from chlamydia infection to PID. For example, if PID occurs at a constant progression rate, PID incidence is 0.007 per year in the control group and 0.0029 per year in the intervention group. A chisquared test with a 5Â % twosided significance level will have 80Â % power to detect this RR of 0.42 with a sample size of 4654 women in each group. With this sample size we would expect 33 PID cases in the control group and 14 PID cases in the intervention group after one year of followup.
Varying the duration of infection from 290 to 440Â days and the fraction of women who develop PID from seven to 13Â % results in a median PID incidence of 0.007 per year (range 0.0041â€“0.0115, Fig.Â 3). The incidence of PID decreases with increasing duration of infection because fewer women in the control group become newly infected during the followup period. PID incidence increases with an increasing fraction of women who develop PID. FigureÂ 4 shows the resulting RR and the sample size needed per group for constant progression and progression at the end. Although the RR decreases with increasing duration of infection (Fig.Â 4a and 4b), the sample size needed per group is almost unaffected by the change in duration of infection (Fig.Â 4c and 4d) or by changing chlamydia prevalence (not shown).
For constant progression from chlamydia infection to PID, the median RR is 0.42 (range 0.36â€“0.49, Fig.Â 4a) with a median sample size needed per group of 4,667 (range 3,644â€“6,657, Fig.Â 4c). For progression at the end, the median RR is 0.39 (range 0.34â€“0.45, Fig.Â 4b) and the corresponding sample size needed per group has a median of 4,149 (range 3,154â€“6,125, Fig.Â 4d). The RR resulting from immediate progression is >1 and the sample size needed per group is >800,000 (see Additional file 1: Figure S5).
The sample size needed per group depends on PID incidence in the control group and the RR. Hence, the observed pattern of sample size needed for different values of the infectious duration and fraction developing PID is a combination of the estimated patterns for PID incidence and RR. We therefore investigated the relation between sample size needed per group, RR, and PID incidence while varying the fraction developing PID. For the hypotheses of immediate progression to PID and progression to PID at the end of chlamydia infection, the RR is independent of the fraction developing PID, i.e. increasing the fraction developing PID increases PID incidence and decreases the sample size needed per group if everything else is kept constant. For constant progression to PID the relationship between sample size needed per group and the fraction developing PID is more complicated because the RR also depends on the fraction developing PID. In this situation, PID incidence and RR need to be investigated together to predict the sample size needed per group while varying the fraction developing PID (see Addition file 1, section 4).
The last analysis in our study showed that there are optimal followup times which minimise the sample size needed per group if all infectious parameters and the fraction developing PID are fixed (Fig.Â 5). For constant progression to PID, a followup time of 294Â days minimises the sample size needed to 4574 per group with a RR of 0.358 and a PID incidence of 0.0056 per year. For progression to PID at the end, a followup time of 328Â days minimises the sample size needed to 4106 per group with a RR of 0.359 and a PID incidence of 0.0063 per year. The immediate progression hypothesis was not further investigated because RRâ€‰>â€‰1.
Discussion and conclusion
This study showed how a mathematical model can be used to inform sample size considerations for RCTs. We used the example of a screening intervention to prevent chlamydia infection and PID as a complication by reexamining published sample size calculations from the POPI trial. Different sets of assumptions about PID incidence and the RR values used for the sample size calculation in the POPI trial required different hypotheses about the temporal relationship between chlamydia and PID. The sample size calculation in the POPI trial using 2Â % PID incidence per year and RRâ€‰=â€‰0.48 was compatible with an assumed constant progression rate from chlamydia infection to PID, whereas with 3Â % PID incidence and RRâ€‰= 0.44 progression from chlamydia to PID would have to occur at the end of the infectious period. In addition, using the outputs of our model in a generic sample size calculation we found large differences in the required sample size between the POPI trial calculation and our model prediction. For example, with a constant progression rate, the median total sample size required was 9,334 and ranged from 7,288 to 13,314, depending on the values of the infection parameters. This is more than double the amount considered by the POPI trial investigators in their first calculation. Not to forget, the RR and the corresponding sample size needed per group depend also on the other natural history parameters of the infection, however, not all infection parameters have the same impact.
The strength of this study was the exploration of three different temporal relationships between chlamydia infection and PID within the same modelling framework. The model does, however, make several simplifying assumptions. First, the three types of progression are hypothetical because the immunological and pathogenetic mechanisms of PID development are still uncertain [11â€“13]. It is not biologically plausible that PID develops either immediately after C. trachomatis infection or just before natural clearance; they represent the extreme consequences of early and late progression [16]. Nevertheless, we found that the type of progression was an important factor in the sample size consideration. Second, the model does not include treatment failure for the intervention group, which would reduce the effect size and result in an increase in sample size. Third, we assumed no change in chlamydia test behaviour during the trial, which could either increase or decrease the effect size. Fourth, we considered a closed population, i.e. no lossto followup, so this would have to be factored into a sample size calculation. Finally, we focused on a SIS model but including an immunity stage did not alter the results in our study about the sample size considerations in the POPI trial (Additional file 1, section 7).
To our knowledge this is the first study to investigate how a mathematical model can be used for sample size calculations in trials of chlamydia infection and PID prevention. Few mathematical modelling studies explicitly state assumptions about the timing of progression from chlamydia to PID, despite their importance in understanding chlamydia infection and disease [7]. Smith and colleagues examined the impact of different intervals between chlamydia infection and PID development on the costeffectiveness of chlamydia screening [24]. Tuite and colleagues incorporated an assumption that PID develops at the midpoint of C. trachomatis infectious period to estimate the burden of chlamydial infection [25]. Gray and colleagues assumed a uniform rate of progression from chlamydia infection to PID in their investigation of the effects of a chlamydia vaccine [26]. The advantage of our approach is that we have compared the implications of different assumptions for RCT design and planning.
The POPI trial data and our modelling study together allow an interpretation of the postulated temporal relationship between chlamydial infection and PID in the context of published RCT evidence. The first set of assumptions used to calculate the required sample size (chlamydia prevalence 7Â %, PID incidence 2Â %, RR 0.48) was compatible with the hypothesis that PID can occur at any time during the infectious period of C. trachomatis. This mechanism is supported by the effects of similar screening and treatment interventions in other RCTs [15, 20, 27]. The observed chlamydia prevalence and PID incidence in the POPI trial were close the actual assumptions but the effect size was smaller, so the trial was underpowered. The second set of assumptions was quite similar (chlamydia prevalence 7Â %, PID incidence 3Â %, RR 0.44) but the mathematical model showed that these conditions could only be satisfied if C. trachomatis progresses to PID at the very end of the infectious period. This hypothesis lacks biological plausibility, given that chlamydial infectious load in the lower genital tract should be lower at the end than the beginning of infection [26]. The results of published RCTs also argue against immediate progression to PID after C. trachomatis infection [15, 20, 27]. Our model allowed us to examine the probability of chlamydia infection progressing to PID. In our model, both sets of sample size assumptions would require a high fraction of chlamydia infection progressing to PID (28.6Â % with yearly PID incidence of 2Â % and 42.9Â % PID incidence 3Â %). Estimates of this size have been used in several costeffectiveness studies [7]. However, two recent modelling studies analysing the results of the POPI trial estimated that 10Â % (95Â % CI 7â€“13Â %) respectively 12Â % (95Â % CrI 2â€“24) of women develop PID [16, 28].
Our approach can be applied to other infections and diseases. The mathematical model introduced in this study could be adapted to other sexually transmitted infections such as Mycoplasma genitalium, for which there is great uncertainty about the natural history, but for which screening interventions have been advocated [29]. The model can also be extended to incorporate different types of infection (e.g. those for which immunity after infection is more important) and other assumptions about the temporal relationship between the infection and the complication. This study has implications for the future planning of RCTs. The relationship between an infection and its disease complications need to be understood before planning intervention trials. The findings of this study suggest that mathematical modelling can be a useful tool for exploring uncertainties about the natural history parameters of an infection, the temporal relationship between the infection and its sequelae, and the implications for sample size calculations in RCTs.
Abbreviations
 Chlamydia:

Chlamydia trachomatis
 CI:

Confidence interval
 CrI:

Credibility interval
 PID:

Pelvic inflammatory disease
 POPI:

Prevention Of Pelvic Infection trial
 RCT:

Randomised controlled trial
 RR:

Relative risk
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Acknowledgements
The authors would like to thank Anne Cori (Imperial College, London, UK) and Regina Riedl (Medical University of Graz, Graz, Austria) for their helpful suggestions.
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Authorsâ€™ contributions
SAH and AB developed the idea for the study and analysed the model. SAH programmed the model and wrote the first draft of the paper. NL contributed to the study design and to the manuscript. All authors commented on the manuscript and approved the final version.
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Additional file 1:
Appendix for method and result section.
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Herzog, S.A., Low, N. & Berghold, A. Sample size considerations using mathematical models: an example with Chlamydia trachomatis infection and its sequelae pelvic inflammatory disease. BMC Infect Dis 15, 233 (2015). https://doi.org/10.1186/s1287901509535
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DOI: https://doi.org/10.1186/s1287901509535