This article has Open Peer Review reports available.
Human papillomavirus (HPV) prevalence and types among Turkish women at a gynecology outpatient unit
© Dursun et al; licensee BioMed Central Ltd. 2009
Received: 13 November 2008
Accepted: 30 November 2009
Published: 30 November 2009
Human Papillomavirus (HPV) is a well-known pathogen for lower genital tract neoplasias, yet little is known regarding HPV prevalence in Turkey. The aim of this study was to investigate the prevalence of HPV DNA and to determine HPV types distribution among women with normal and abnormal cytology.
A total of five hundred seven (n = 507) women were retrospectively evaluated between 2004-2008. Conventional polymerase chain reaction was used to detect the presence of HPV types in cervicovaginal samples obtained from patients during gynecologic examination.
One hundred four (n = 104) of the women were excluded from the study because of the incomplete data and a total of 403 women were used for the final analysis. There were, 93 (23%) women with cytologic abnormality and 310 (77%) women with normal cytology. Overall, 23% of the women was HPV positive. The overall prevalence of HPV in women with abnormal Pap smears was 36% (93/403), of which in ASCUS 22%, LSIL 51% and HSIL 60%. Also, HPV DNA was positive in 20% of the women with normal cervical cytology. The most common HPV types in cytologically normal women were as follows; HPV 16 (36%), HPV 6 (22%) and HPV 18 (13%). The rate of other HPV types were as follows; HPV11 4.4%, HPV45 4.4%, HPV90 4.4%, HPV35 2.2%, HPV67 2.2%, HPV81 2.2%, and multiple type HPVs 8.9%. The most common HPV types in cytologically abnormal women were HPV 16 (35%), HPV6 (19%) and HPV18 (8%). The rate of multiple HPV infections in women with normal Pap test was 2.2%.
HPV prevalence and type distribution in this study were similar to that reported worldwide at least in our study population. Hovewer, HPV prevalence was more common compared with previous studies reported from Turkey. This might be related with methodology and hospital based patient accrual and high rate of women with abnormal cytology. Further population based prospective studies are needed to eliminate the drawbacks of our study and to determine nonhospital based HPV prevalence in Turkish women.
Cervical cancer is the one of the leading cause of cancer-related deaths among women worldwide. It is e estimated 500,000 cases occurring annually and a 50% case fatality rate .
According to statistics of Ministry of Health and GLOBOCAN 2002, cervical cancer is the 9th most common cancer among women in Turkey and ranks 13th among cancer-related deaths, with an estimated 1364 cases occurring annually and more than 50% of fatality rate .
Today, it is widely accepted that Human papillomavirus virus (HPV) is the main causal factor of cervical carcinoma. More than 100 HPV genotypes have been described and nearly 20 of them have been associated with cervical carcinoma. It has been reported that HPV prevalence range between 1.4% to 25.6% in the world . Previous studies reported that HPV prevalence between 2% to 6% among the low risk women in Turkey [4–8]. However, these studies generally included small number of cases or performed by using Hybrid Capture I or II. The aim of this study is to investigate the HPV prevalence by using PCR method and to report the rate of HPV infection in women with normal and abnormal cervical cytology.
Between January 2004 and August 2008, 503 consecutive sample of women attending gynecological outpatient clinics for regular gynecologic control in Baskent university School of Medicine, Departments of Obstetrics and Gynecology were included to this retrospective analysis. Women were eligible if they had no previous diagnosis or treatment for cervical, vulvar or vaginal cancer and ever sexually active. Also, patients with previous history of chemoradiotherapy for cervical carcinoma were excluded from the analysis. Information on sociodemographic characteristics, medical history and sexual and reproductive behaviour was obtained at the time of the gynecological visit which was filled during the gynecological visit. Samples of exfoliated cervical cells were obtained using a cytobrush. After the preparation of a standard cervical smear, the remaining cells were placed in tubes with 0.9% saline and stored at - 201C until shipment to the our microbiology laboratory. Pap smears were classified according to the Bethesda system by pathology department. HPV DNA detection and genotyping performed by PCR in our microbiology labaratory as described previously . The data were analysed by of the t test, continuity-corrected chi-square method or Fisher's exact test, One way Anova, calculation of prevalence ratio and descriptive statistics as required, and the probability type I error was set at 0.05 (two-tailed). Prevalence ratio for each decads (2th, 4th and 5th) calculated by comparing with 3rd decad using poisson regression with robust variance estimates as described previously [10, 11] Statistical analysis performed by SPSS v.11.5.
HPV DNA detection and genotyping
HPV DNA detection in cervical swabs was conducted by using real-time polymerase chain reaction (PCR) with a commercial kit (Fluorion, Iontek, Turkey). For DNA extraction the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) was used in accordance with the instructions of the manufacturer. A 150 bp fragment of the L1 gene was amplified using GP5 and GP6 primers. An amplified gene product was identified via melting curve analysis and visualized by incorporation of Sybr Green dye during amplification. HPV genotyping was performed with DYEnamic ET Terminator Cycle Sequencing Kit (Amersham Biosciences Corp., NJ, USA) and ABI PRISM 310 Genetic Analyzer at Iontek Ltd, Turkey.
The study protocol was approved by at Ethical Board of Baskent University, School of Medicine.
During the four year period, 503 women who attend for regular gynecological examination were analyzed for HPV in our departments. One hundred four of these women were excluded from the analysis because of the incomplete data and a total of 403 women were used for the final analysis.
Mean age of the patients was 37.5 ± 9.3 (range 19-67 y). Ninety percent of the population was premenaposal while 10% was menopausal. There were significant differences with respect to HPV positivity between women under 30 years old and women older than 30 years old (34% vs.20%, p = 0.005) but, there was no significant differences with respect to decads of life and HPV positivity. Also, menopausal status has no effect on HPV positivity. HPV was positive in 24% of the premenopausal women and 17% of the menopausal women (p = 0.323).
Distribution of cervical cytology and HPV in the study population
No. of Specimens
Overall HPV (+)
38.1 ± 9.4 (20-67)
35.6 ± 8.7
37.0 ± 8.8
33.6 ± 8.9
38.0 ± 6.9
HPV type distribution in cases with normal and abnormal cytology
HPV Types in All Positive Patients (n = 93)
(n = 310)
Abnormal Cytology *
(n = 93)
HPV prevalence by age in women with normal cervical cytology (n = 310)
HPV (+)/total no. of patients
Today, it is well known that some kind of cancers are closely related with persistent infection of oncogenic types HPV. On the basis of epidemiological and virological studies, HPV is estimated to cause 100% of cases of cervical cancer, 90% of anal cancer, 40% of cancers of the external genitalia (vulva, vagina and penis), at least 12% of oropharyngeal cancers and 3% of oral cancers . It has been estimated that HPV is responsible for 5.2% of all cancers worldwide . Furthermore, Human papillomavirus (HPV) has been established as a necessary cause of cervical cancer and its precursors, including cervical intraepithelial neoplasias grades 2 and 3 (CIN 2-3). Therefore, detection of high-risk HPV is becoming increasingly attractive as a primary screening tool. Also, data for HPV-type distribution is sine qua non to development of new HPV-screening tests and to assessment of the effect of future vaccination on HPV infections of differing severity, but published data about the HPV prevalence in Turkey limited [3–8].
HPV studies reported from Turkey
Ege SSK Hospital, Ýzmir 
Hacettepe University Hospital, Ankara 
Erciyes University Hospital, Kayseri 
Mean of HCs
Numune Hospital, Ankara 
Hacettepe University Hospital-2, Ankara 
Baskent University Hospital, Ankara (Current study)
Mean of PCRs
In 2007, Sanjosé et al. reported that overall HPV prevalence in 157.879 women with normal cervical cytology was estimated to be 10.4% in a meta-analysis of 78 studies . In 2008, Bao et al. reported that overall HPV prevalence was 14.4% in Asia in a meta-analysis of 79 studies which including 25,368 women . In this study, for women with normal cytology/histology, HPV prevalence had a smaller range of 14.0% in southeastern Asia to 14.4% in south central Asia. On the other hand, in an another meta-analysis including the results of 15.613 women, overall HPV prevalence was 8.7%,14.3% and 5.2% for Asia, South America and Europe, respectively . In our study, the rate of HPV positivity in women with normal cervical cytology was 20%. Variations between studies most likely reflect differences in the population studied with respect to risk factors for exposure to HPV and methods of evaluations.
In women with abnormal cytology results, the presence of HPV infection has been reported in 28.8%-61.3% of cases [18–22]. In our study, HPV was positive in 36% of women with abnormal cytology. This low rate of HPV in women with abnormal cytology could be explained by the false evaluation of the Pap test of women which was referred to our instution. HPV infection by multiple genotypes has been reported to occur in 10% to 20% of HPV-positive cases and to show dependence on HPV type, age of the patient, and the presence of cytologic abnormalities . Interestingly, we also found that 8.9% of HPV-infected women has more than one HPV genotype in women with normal cervical cytology while this percentage was 11.8 in women with abnormal cervical cytology (p > 0.05). In other studies, this figures varies between 7% and 23% depending on the cytological diagnosis. Double infection with HPV 16 and 18 was found 6.6% in women with normal cervical cytology. Arora et al. reported the 6.7% of the women with normal cytology had double infection with HPV 16 and 18 . Therefore, Arora et al suggested that the cases who are HPV positive for any of the high risk types but have concurrently negative Pap smear or no visible lesions should be followed up more frequently than the HPV negative cases as they have a substantially greater risk of developing an abnormal smear [24, 25]
Some epidemiologic studies reported that a significant reduction in HPV prevalence throughout the third decade of life . It has also been reported that HPV prevalence is between 32-64% in women under 25 years-old women while this figure between 2-4% in women older than 45 years old . Similarly, in our study the rate of HPV infection was 34% in women under 30 years old and this rate was significantly higher than women older than 30 years old. Hovewer, there was no significant differences in HPV prevalence by decads of life in patients with normal cervical cytology (Table 3). This situation also needs to be explanation. On the other hand, it is weel known, HPV-16 is the most prevalent type worldwide. However, the second most prevalent one is HPV-18 in Western countries, whereas it is HPV-58 in Asia . HPV 16, 6 and 18 were the most common three type in our study population as in western countries . On the other hand, HPV prevalence was reported to be 81.0% in women with HSIL. The ten most common HPV types were 16, 58, 52, 18, 33, 51, 31, 56, 35, and 45 in HSIL . Onan et al from Turkey reported that 45% of the women with cervical intraepithelial neoplasia (CIN) had high risk HPV . Inal et al. performed HC-II study in 1353 women from Izmir region which has the highest cervical cancer incidence in Turkey, and reported 2.1% women had positive for HPV. Hovewer, all the women with cytological abnormalities had positive for HPV DNA. Therefore, the authors concluded that "In spite of low general frequency of cervical HPV colonization, there was a strong correlation between HPV and CIN" . In our study, HPV was positive in 36% of the cases with cytological abnormalities.
In conclusion, although geographicly Turkey is located between Europe and Asia, HPV prevalence and genotypes are similar to those reported from western countries in Turkish women with normal and abnormal cytology. Hovewer, HPV prevalence was more common compared with previous studies reported from Turkey. This might be related with methodology and hospital based patient accrual and high rate of women with abnormal cytology. Further population based prospective studies are needed to eliminate the drawbacks of our study and to determine nonhospital based HPV prevalence in Turkish women.
- Bulletin of the World Health Organization (BLT): World Health Organization Web site. [http://www.who.int/bulletin/volumes/84/2/]
- Turkish national cancer advisory board. [http://ukdk.org/pdf/kitap/en/30.pdf]
- Clifford GM, Gallus S, Herrero R, Muñoz N, Snijders PJ, Vaccarella S, Anh PT, Ferreccio C, Hieu NT, Matos E, Molano M, Rajkumar R, Ronco G, de Sanjosé S, Shin HR, Sukvirach S, Thomas JO, Tunsakul S, Meijer CJ, Franceschi S, IARC HPV Prevalence Surveys Study Group: Worldwide distribution of human papillomavirus types in cytologically normal women in the International Agency for Research on Cancer HPV prevalence surveys: a pooled analysis. Lancet. 2005, 366 (9490): 991-8. 10.1016/S0140-6736(05)67069-9.View ArticlePubMedGoogle Scholar
- Inal MM, Köse S, Yildirim Y, Ozdemir Y, Töz E, Ertopçu K, Ozelmas I, Tinar S: The relationship between human papillomavirus infection and cervical intraepithelial neoplasia in Turkish women. Int J Gynecol Cancer. 2007, 17 (6): 1266-70. 10.1111/j.1525-1438.2007.00944.x. Epub 2007 Apr 18View ArticlePubMedGoogle Scholar
- Tuncer ZS, Basaran M, Ustaçelebi S, Kuzey GM: High-Risk Human Papilloma Virus (HPV) Infection Determined By Hybrid Capture II Assay in a Turkish University Hospital Outpatient Clinic MN-Gorm. 2006, 12 (2): 129-134.Google Scholar
- Ozçelik B, Serin IS, Gökahmetoğlu S, Başbuğ M, Erez R: Human papillomavirus frequency of women at low risk of developing cervical cancer: a preliminary study from a Turkish university hospital. Eur J Gynaecol Oncol. 2003, 24 (2): 157-9.PubMedGoogle Scholar
- Seçkin S, Aksoy F, Yıldırım M: Servikal Smearlerde HPV İnfeksiyonu Görülme İndisansı. Ankara Numune Eğitim ve Araştırma Hastanesi Tıp Dergisi. 1996, 36 (1-2-3-4): 101-103.Google Scholar
- Safi Z, Demirezen S, Beksaç MS, Kuzey GM, Kocagöz T, Ustaçelebi Ş, Hasçelik G, Çakar An: İnsan Papilloma Virusunun (IPV) Polimeraz Zincir Reaksiyonu Tekniği İle Servikal Ve Vajinal Akıntı Örneklerinde Saptanması. Mn-Klinik Bilimler&Doktor. 2002, 8 (1): 112-114.Google Scholar
- Evans MF, Adamson CS, Papillo JL, St John TL, Leiman G, Cooper K: Distribution of Human Papillomavirus Types in ThinPrep Papanicolaou Tests Classified According to the Bethesda 2001 Terminology and Correlations with Patient Age and Biopsy Outcomes. Cancer. 2006, 106: 1054-64. 10.1002/cncr.21664.View ArticlePubMedGoogle Scholar
- Spiegelman D, Hertzmark E: Easy SAS calculations for risk or prevalence ratios and differences. Am J Epidemiol. 2005, 162: 199-200. 10.1093/aje/kwi188.View ArticlePubMedGoogle Scholar
- Lee J, Tan CS, Chia KS: A practical guide for multivariate analysis of dichotomous outcomes. Ann Acad Med Singapore. 2009, 38 (8): 714-9.PubMedGoogle Scholar
- Parkin DM, Bray F: The burden of HPV-related cancers. Vaccine. 2006, 24 (Suppl 3): S11-S25. 10.1016/j.vaccine.2006.05.111.View ArticleGoogle Scholar
- Parkin DM: The global health burden of infection-associated cancers in the year 2002. Int J Cancer. 2006, 118: 3030-44. 10.1002/ijc.21731.View ArticlePubMedGoogle Scholar
- Prevalence of Abnormal Cervical Cytology in Turkey: A Turkish Gynecologic Oncology Group study. Turkish Cervical Cancer and Cervical Cytology Research Group. Int J Gynaecol Obstet. 2009, 106 (3): 206-9. 10.1016/j.ijgo.2009.04.003.View ArticleGoogle Scholar
- Dursun P, Altuntas B, Kuscu E, Ayhan A: Women's Knowledge about Human Papilloma Virus (HPV) and Their Acceptance of HPV Vaccine. Aust N Z J Obstet Gynaecol. 2009, 49 (2): 202-6. 10.1111/j.1479-828X.2009.00947.x.View ArticlePubMedGoogle Scholar
- Bao YP, Li N, Smith JS, Qiao YL, ACCPAB members: Human papillomavirus type distribution in women from Asia: a meta-analysis. Int J Gynecol Cancer. 2008, 18 (1): 71-9. Epub 2007 Apr 26View ArticlePubMedGoogle Scholar
- de Sanjosé S, Diaz M, Castellsagué X, Clifford G, Bruni L, Muñoz N, Bosch FX: Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis. Lancet Infect Dis. 2007, 7 (7): 453-9. 10.1016/S1473-3099(07)70158-5.View ArticlePubMedGoogle Scholar
- González-Bosquet E, Esteva C, Muñoz-Almagro C, Ferrer P, Pérez M, Lailla JM: Identification of vaccine human papillomavirus genotypes in squamous intraepithelial lesions (CIN2-3). Gynecol Oncol. 2008, 111 (1): 9-12. 10.1016/j.ygyno.2008.06.012. Epub 2008 Aug 5View ArticlePubMedGoogle Scholar
- Ciotti M, Sesti F, Paba P, Benedetto A, Patrizi L, Criscuolo A, et al: Human papillomavirus (HPV) testing in the management of women with abnormal pap smears. Experience of a colposcopy referral clinic. Eur J Gynaec Oncol. 2004, 25: 577-84.Google Scholar
- Rowe LR, Aldeen W, Bentz JS: Prevalence and typing of HPV by hybrid capture in women with ASCUS, ASC-H and AGC on ThinPrep Pap test. Diagn Cytopathol. 2004, 30: 426-32. 10.1002/dc.20052.View ArticlePubMedGoogle Scholar
- González Bosquet E, Muñoz Almagro MM, Mora I, Suñol M, Callejo J, Lailla JM: Prevalence of human papilloma virus infection of the uterine cervix in women with abnormal cervical cytology. Eur J Gynaecol Oncol. 2006, 27 (2): 135-8.PubMedGoogle Scholar
- Levert M, Clavel C, Graesslin O, Masure M, Birembaut P, Quereux C, et al: Human papillomavirus typing in routine cervical smears. Result from a series of 3778 patients. Gynecol Obstet Fertil. 2000, 28: 722-8. 10.1016/S1297-9589(00)00011-4.View ArticlePubMedGoogle Scholar
- Hwang HS, Park M, Lee SY, Kwon KH, Pang MG: Distribution and Prevalence of Human Papillomavirus Genotypes in Routine Pap Smear of 2,470 Korean Women Determined by DNA Chip. Cancer Epidemiol Biomarkers Prev. 2004, 13 (12): 2153-6.PubMedGoogle Scholar
- Arora R, Kumar A, Prusty BK, Kailash U, Batra S, Das BC: Prevalence of high-risk human papillomavirus (HR-HPV) types 16 and 18 in healthy women with cytologically negative Pap smear. Eur J Obstet Gynecol Reprod Biol. 2005, 121 (1): 104-9. 10.1016/j.ejogrb.2004.11.025.View ArticlePubMedGoogle Scholar
- Woodman CB, Collins S, Winter H, et al: Natural history of cervical human papillomavirus infection in young women: a longitudinal cohort study. Lancet. 2001, 357 (9271): 1831-6. 10.1016/S0140-6736(00)04956-4.View ArticlePubMedGoogle Scholar
- Sellors JW, Mahony JB, Kaczorowski J, et al: Prevalence and predictors of human papilloma virus infection in women in Ontario, Canada. Survey of HPV in Ontario Women (SHOW) Group. CMAJ. 2000, 163: 503-8.PubMedPubMed CentralGoogle Scholar
- Burk RD, Kelly P, Feldman J, et al: Declining prevalence of cervicovaginal human papillomavirus infection with age is independent of other risk factors. Sex Transm Dis. 1996, 23: 333-341.View ArticlePubMedGoogle Scholar
- Onan MA, Taskiran C, Bozdayi G, Biri A, Erdem O, Acar A, Gunaydin G, Rota S, Ataoglu O, Guner H: Assessment of human papilloma viral load of archival cervical intraepithelial neoplasia by real-time polymerase chain reaction in a Turkish population. Eur J Gynaecol Oncol. 2005, 26 (6): 632-5.PubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/9/191/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.