Estimating Chikungunya prevalence in La Réunion Island outbreak by serosurveys: Two methods for two critical times of the epidemic
© Gérardin et al; licensee BioMed Central Ltd. 2008
Received: 03 September 2007
Accepted: 28 July 2008
Published: 28 July 2008
Chikungunya virus (CHIKV) caused a major two-wave seventeen-month-long outbreak in La Réunion Island in 2005–2006. The aim of this study was to refine clinical estimates provided by a regional surveillance-system using a two-stage serological assessment as gold standard.
Two serosurveys were implemented: first, a rapid survey using stored sera of pregnant women, in order to assess the attack rate at the epidemic upsurge (s1, February 2006; n = 888); second, a population-based survey among a random sample of the community, to assess the herd immunity in the post-epidemic era (s2, October 2006; n = 2442). Sera were screened for anti-CHIKV specific antibodies (IgM and IgG in s1, IgG only in s2) using enzyme-linked immunosorbent assays. Seroprevalence rates were compared to clinical estimates of attack rates.
In s1, 18.2% of the pregnant women were tested positive for CHIKV specific antibodies (13.8% for both IgM and IgG, 4.3% for IgM, 0.1% for IgG only) which provided a congruent estimate with the 16.5% attack rate calculated from the surveillance-system. In s2, the seroprevalence in community was estimated to 38.2% (95% CI, 35.9 to 40.6%). Extrapolations of seroprevalence rates led to estimate, at 143,000 and at 300,000 (95% CI, 283,000 to 320,000), the number of people infected in s1 and in s2, respectively. In comparison, the surveillance-system estimated at 130,000 and 266,000 the number of people infected for the same periods.
A rapid serosurvey in pregnant women can be helpful to assess the attack rate when large seroprevalence studies cannot be done. On the other hand, a population-based serosurvey is useful to refine the estimate when clinical diagnosis underestimates it. Our findings give valuable insights to assess the herd immunity along the course of epidemics.
In La Réunion, the epidemic pattern was monitored through a regional surveillance-system managed by the Cellule Interrégionale d'Epidémiologie (CIRE) based on "suspected cases", defined as subjects with a sudden fever (T > 38.5°C) and incapacitating arthralgia [10, 11]. This surveillance-system relied on self-reports, emergency stays, physician declarations, biology laboratories activity, and active case-finding around the cases reported by a sentinel physician network . At the beginning of the outbreak it consisted in an active and retrospective case detection around the cases declared, and then, when the incidence sharply increased (by December 2005), in an estimation of the cases obtained from reports of a sentinel network .
The purpose of the study was to refine the estimates of attack rates provided by the surveillance-system for the population of La Réunion Island at two critical times of the 2005–2006 outbreak. That is why we conducted two serosurveys, the first using stored sera of pregnant women during the epidemic upsurge aimed at assessing the extent of the outbreak, the second using a random sample of the population aimed at giving a precise idea of the herd immunity in the post-epidemic era.
Rapid survey on pregnant women, epidemic phase
We gathered sera of pregnant women yet available in outpatient laboratories from a mandatory monthly serological screening for congenital toxoplasmosis. The sera, collected between January 15th and February 15th 2006, were neither directly nor indirectly nominative, and could only be identified by a unique code number. All of the 46 biological labs of La Réunion Island were invited to participate to the survey. Out of these, the 28 participating ones served the entire territory (Figure 1). However, only 19 labs provided valid sera which led to a non-representative amount of 888 valid sera, taken out of the 3888 sera collected during the study period (389 in the north, 305 in the south, 174 in the west, 20 in the east). For this study, designed to inform without delay public health authorities on the extent of the outbreak, a selection bias related to the absence of randomisation of labs was tolerated. Nevertheless, as daily routine sampling of pregnant women was not dedicated to a precise laboratory, it is reasonable to think that the repartition bias was not significant. Statistical analysis was performed using SAS software version 8 (SAS Institute, Cary, NC).
Population-based survey, post-epidemic phase
A cross-sectional study, the SEROCHIK survey, was conducted between August 17th and October 20th 2006 from a random sample of the community of Reunion Island . At the first sampling stage, the French National Institute for Statistics and Economical Studies (INSEE) randomly selected 3032 households after stratification on age, gender, the geographical area, municipality size, and type of habitat. The geographical area of habitat was defined according to the regional administrative boundary into four micro-regions (Figure 1). The municipality size was divided in ≤ or > 10,000 inhabitants. The type of habitat was categorized into individual or collective housing (multifamily ≤ 20 or > 20 housings). At the second stage, a Kish method was used to randomly choose one person for each selected household .
Of the 3032 households randomly selected by INSEE, the sampling plan led to a set of 2442 eligible subjects (after exclusion of absents, persons with invalid address or who refused to participate) which was recovered by INSEE on age, gender, geographical area, and type of habitat.
The study was approved by the ethical committee for studies with human subjects (CPP) of Bordeaux and the National Commission for Informatics and Liberty (CNIL). All participants provided their informed consent to answer the questionnaire and for collection of blood on filter paper.
Statistical analysis was done by accounting for the sampling design, and was performed using Stata software (College Station, Texas). The population-based seroprevalence was compared to CIRE clinical estimates using a Chi square test. A P-value < 0.05 was considered significant. The population size used for the calculation of incidence was 787,836 inhabitants (INSEE, April 2006).
Detection of chikungunya infections
For the rapid survey in pregnant women, 100 microliters of stored sera already available in outpatient laboratories were used. In the SEROCHIK survey, for each person consenting to a fingertip prick, a drop of blood was deposited onto Whatman no.1 filter paper . For both studies, anti-CHIKV specific antibodies were screened using the same enzyme-linked immunosorbent assay (ELISA) and a CHIKV antigen produced by the Centre National de Référence pour les Arbovirus (CNR, Lyon, France) . For the rapid survey, the ELISA was performed at the CNR whereas for the SEROCHIK survey, it was done using the Groupe Hospitalier Sud – Réunion (GHSR) laboratory facilities. Both IgM and IgG anti-CHIKV specific antibodies were screened in sera from pregnant women, whereas only IgG anti-CHIKV specific antibodies were screened in the community. In parallel with the SEROCHIK survey, the ability of the prick-method to discriminate the serological status was validated in an independent sample (Fianu et al, unpublished data).
Chikungunya serological status during the epidemic upsurge phase (rapid survey) and the post-epidemic era (population-based survey), Reunion Island outbreak, 2005 – 2006.
Pregnant women (rapid survey)
Population (population – based survey)
Survey on pregnant women, epidemic phase
During the studied period (epidemic phase, Figure 2), 162 pregnant women (out of 888 enrolled, i.e. 18.2%) tested positive for antibodies to CHIKV (IgM and/or IgG). There was serological evidence for a recent Chikungunya infection, as 123 subjects (13.8%) showed both IgM and IgG, and 38 (4.3%) had IgM in the absence of IgG. Isolated positive IgG were detected in only one case (0.1%).
Population-based survey, post-epidemic phase
Chikungunya clinical status × serological status in the community.
Population-based survey, Reunion Island outbreak, August – October 2006 (post-epidemic era)
"I don't know"
As the CIRE only considered suspected cases, i.e. subjects with fever above 38.5°C with incapacitating arthralgia, we also took into account the symptoms reported in the questionnaire of the SEROCHIK population-based survey.
Chikungunya self-reported symptoms × serological status in the community.
Population-based survey, Reunion Island outbreak, August – October 2006 (post-epidemic era)
Positive serology *
Fever and arthralgia
In the current paper, we demonstrate the usefulness of two different epidemiological methods to assess the burden of a Chikungunya outbreak at two critical times of its evolution.
The rapid seroprevalence survey conducted at the peak of La Réunion Island epidemic on sera from pregnant women provided an 18.2% seroprevalence rate (or a rough estimate of 143,000 people infected) by February 15th, 2006. This result was obtained at a very low cost, at a time when the attack rate of the infection in the population and the herd immunity were unknown. Since 99% of the 162 tested sera harbored IgM anti-CHIKV antibodies and only one IgG anti-CHIKV antibodies only, it excluded a previous (recent) significant circulation of the virus in the island and thus suggested that the outbreak emerged into a naïve population. This result is in agreement with the 20% prevalence rate calculated for all parturient women delivered at the GHSR maternity in mid-February 2006 , and with the magnitude of 26% reported in pregnant women by April 2006 in Mayotte .
The rough estimate by the rapid serosurvey in pregnant women is slightly higher but of the same magnitude than the 130,000 cumulative number of suspected cases deducted from the CIRE at the same time (attack rate of 16.5%) [18, 19]. It is therefore noteworthy that the rate observed in a targeted population of pregnant woman gives a valuable insight upon the magnitude of the attack rate in the community, and beyond, of the herd immunity. This congruent result with the CIRE data suggests that in February 2006 pregnant women yet behaved like everyone and that their level of exposure to CHIKV was not different to that observed for anybody else. In other words, the message aimed at crystallising the pregnant woman as vulnerable person to the threat of Chikungunya  and the measures aimed at reducing her exposure , e.g., wear of long clothing, free distribution of insecticide-treated nets and repellents, soon implemented in the maternities of La Réunion, were not effective against A. albopictus bites, a vector which was proven to exercise a diurnal activity .
The slight difference between pregnant women and community may account for a selection phenomenon, the pregnant women being not representative of the community. However, the prevalence was higher in pregnancy which argues against a significant selection bias, since more than 50% of serum collections came from south and west labs at a time when transmission was still predominant in theses micro-regions, leading to an unexpected geographical adjustment on the transmission level.
The population-based survey provided a 38.2% prevalence rate in the post-epidemic era, i.e., about 300,000 people infected, by October 20, 2006 . At the same time, the CIRE data, published on the Institut national de Veille Sanitaire website, estimated at 266,000 (34.3%) the number of people infected . Thus, the seroprevalence in the community was slightly higher than the 34.3% estimated for suspected cases (P < 0.001), but of the same magnitude. This difference might correspond to undeclared cases to the CIRE, i.e., (1) the inapparent cases (5.0%), although these would be compensated by an approximately equal proportion of false positives (4.5%); (2) patients who did not consult and performed auto-medication; (3) patients who did not match the clinical criteria for "suspected cases", i.e., sudden fever > 38.5 C° and incapacitating arthralgia. Based on laboratory confirmations of atypical presentations, Renault et al. calculated that approximately 3% of patients did not fulfil this definition . Importantly, the SEROCHIK survey showed that 25.8% of CHIKV-infected subjects did not declare fever combined to arthralgia. This significant discrepancy may result both from an information bias due to the structure of the questionnaire (subjects who were not aware of their infection did not answer to questions about clinical signs), or a memory bias due to the time interval between the SEROCHIK survey and the onset of symptoms (2 to 15 months) that could preclude mild cases to remind their symptoms. However, the declaration-based surveillance system based on suspected cases may have also underestimated the attack rate at the epidemic phase. The difference was particularly notable from April to December 2005 (Figure 3), when the incidence was less than 500 new cases per week (Figure 2) and the surveillance relied upon active and retrospective case detection around the cases declared. Ditto, it was verified from June 2006 (Figure 3), when the incidence dropped dramatically shortly before the epidemic stopped in August (Figure 2). It could be explained by a lower PPV for each symptoms (< 85 to 92%) and for the clinical definition (< 87%), as the transmission was low (Table 3). Indeed, it is well known that when the incidence of a communicable disease is low, its contribution to the clinical forms that can evoke it decreases, with a consequent decline of the PPV for clinical signs to identify the disease . Moreover, the possibility of concomitant circulation of other infections, such as Influenza or Dengue , may have challenged the diagnosis of Chikungunya , especially between April and December 2005, or from June 2006.
Another important aspect of Chikungunya disease disclosed in the course of La Réunion outbreak is the low proportion of inapparent forms (16%), in comparison with those usually observed for other arbovirosis, such as Dengue Fever (> 50%) [25, 26] or West-Nile virus infection (> 70%) [27, 28].
Finally, it is noteworthy that the 38.2% seroprevalence rate observed in the post-epidemic phase  gives a valuable insight upon the herd immunity and a clearer picture of susceptible people who could be infected in the future (62.8%). Importantly, the seroprevalence observed in La Réunion Island was far inferior to those reported recently from the Kenyan island of Lamu (75%)  and the Grande Comoro Island (63%) , two areas where CHIKV emerged before to reach La Réunion [4, 5].
Several hypotheses may explain this discrepancy in prevalence rates: 1°) Kenyan and Comorian climates are less prone to seasonal variations and therefore more conducive to a sustainable transmission; 2°) A. aegypti, the classical vector of Chikungunya, involved in Kenyan and Comorian outbreaks, keeps a better capability to spread the disease in domestic environment than the less anthropophilic A. albopictus; 3°) for the same reason, the density of susceptible hosts would be less important in peridomestic environment in La Réunion, than in Kenyan and Comorian homes invaded by highly anthropophilic A. aegypti; 4°) in La Réunion, floods brought by the cyclone Diwa drove away larvae of A. albopictus from gullies and hastened the decrease in transmission from early March 2006; 5°) effective vector control measures combining eradication of breeding sites, adulticide and larvicide treatments contributed to limit the density of vectors throughout the Reunion outbreak; 6°) In La Réunion, the herd immunity was gained more rapidly in the littoral plains (where most of the population lives in contact with vectors) which reduced transmission as the entry in the dry austral winter. Indeed, some micro-geographical differences in prevalence rates would have been not detected by the SEROCHIK survey (whose sampling plan was aimed at discriminating between micro-regions but not within), and thereby prevalence rates in littoral plains would have far exceeded the overall 38.2% rate, which would have led to the premature decline of the epidemic due to effective herd immunity.
Prevalence rates of 60 to 70% were necessary to delay resurgences to 20 to 30 years in areas where CHIKV had circulated before . According to a recent mathematical model , it was concluded that in the best-fitting case (reproductive number of 3.7), the attack rate would have been of 73% which suggests that, with only 38.2% of people infected, a re-emergence in La Réunion Island cannot be excluded for the next years, as long as viral strains circulate in the region. However, the scenario observed in La Réunion seems thwart this mathematical prediction, because so far, no case of Chikungunya has been scientifically confirmed since August 2006. This could be explained at municipality level by heterogeneity of reproductive numbers that would have been very sensitive to local interventions in vector control .
Congruent estimates of Chikungunya attack rate were observed at the upsurge of La Réunion Island outbreak, either using clinical declaration of suspected cases (16.5%), or a rapid serosurvey in pregnant women (18.2%). In contrast, a discrepancy was observed in the post-epidemic era, when clinical diagnosis underestimated the attack rate (34.3%) in comparison to seroprevalence estimate (38.2%; 95% CI, 35.9 to 40.6%). Thus, a rapid serosurvey in a targeted population can be helpful to assess the extent of epidemics at time of emergency when large seroprevalence studies cannot be done. Beyond this indication, our findings suggest that prospective real time surveillance of attack rates in pregnant women would serve as a good model for population monitoring in the event of Chikungunya outbreaks. However, although it may fail to detect micro-geographical differences in prevalence rates at municipality level, a population-based serosurvey can still be useful to refine the clinical estimates and to assess more precisely the herd immunity. Moreover, only a representative survey can bring an overview on risk factors and other conditions facilitating the transmission. Finally, this work speaks to the usefulness of serosurveys for the quantification of epidemics, as to cost-containment in public health. It also provides valuables clues for monitoring epidemics in high and low-incomes countries.
We are grateful to the population of La Réunion Island for having been so co-operative. We are indebted to Claude Parain, Head of the "Statistiques et Diffusion" Department at the Institut National des Statistiques et des Sciences Economiques for his advices in designing the sampling plan, and to the Cellule Interrégionale d'Epidémiologie for conducting surveillance for Chikungunya and giving valuable information. We also wish to thank the technicians of the Centre d'Investigation Clinique – Épidémiologie Clinique who collected the sera samples, those of Groupe Hospitalier Sud – Réunion and Institut Pasteur laboratories who analysed the sera, and all the physicians who collectively contributed to strengthen the present paper.
- Johnston RE, Peters CJ: Alphaviruses. Fields Virology. Edited by: Fields BN, Knipe DM, Howley PM. 1996, Philadelphia: Lippincott-Raven, 843-898. 3Google Scholar
- Laras K, Sukri NC, Larasati RP, Bangs MJ, Kosim R, Djauzi , Wandra T, Master J, Kosasih H, Hartati S, Beckett C, Sedyaningsih ER, Beecham HJ, Corwin AL: Tracking the re-emergence of epidemic chikungunya virus in Indonesia. Trans R Soc Trop Med Hyg. 2005, 99: 128-141. 10.1016/j.trstmh.2004.03.013.View ArticlePubMedGoogle Scholar
- Ross RW: The Newala epidemic. III. The virus: isolation, pathogenic properties and relationship to the epidemic. J Hyg (Lond). 1956, 54 (2): 177-191.View ArticleGoogle Scholar
- Schuffenecker I, Iteman I, Michault A, Murri S, Frangeul L, Vaney MC, Lavenir R, Pardigon N, Reynes JM, Pettinelli F, Biscornet L, Diancourt L, Michel S, Duquerroy S, Guigon G, Frenkiel MP, Bréhin AC, Cubito N, Desprès P, Kunst F, Rey FA, Zeller H, Brisse S: Genome microevolution of chikungunya viruses causing the Indian Ocean outbreak. Plos Med. 2006, 3: e263-10.1371/journal.pmed.0030263.View ArticlePubMedPubMed CentralGoogle Scholar
- Charrel RN, de Lamballerie X, Raoult D: Chikungunya outbreaks – the globalization of vectorborne diseases. N Engl J Med. 2007, 356: 769-771. 10.1056/NEJMp078013.View ArticlePubMedGoogle Scholar
- Reiter P, Fontenille D, Paupy C: Aedes albopictus as an epidemic vector of chikungunya virus: another emerging problem?. Lancet Infect Dis. 2006, 6: 463-464. 10.1016/S1473-3099(06)70531-X.View ArticlePubMedGoogle Scholar
- Perrau J, Catteau C, Michault A, Parain C, Favier F: Fin 300000 personnes avaient été atteintes par le Chikungunya. Economie de la Réunion. 2007, 129: 16-17. [http://www.insee.fr/fr/insee_regions/reunion/prodser/pub_elec/revue/revue129/revue129_chikungunya.html]Google Scholar
- Borgherini G, Poubeau P, Staikowsky F, Lory M, Le Moullec N, Becquart JP, Wengling C, Michault A, Paganin F: Outbreak of chikungunya on reunion island: early clinical and laboratory features in 157 adult patients. Clin Infect Dis. 2007, 44: 1401-1407. 10.1086/517537.View ArticlePubMedGoogle Scholar
- Staikowsky F, Le Roux K, Schuffenecker I, Laurent P, Grivard P, Develay A, Michault A: Retrospective survey of Chikungunya disease in Reunion Island hospital staff. Epidemiol Infect. 2008, 136 (2): 196-206. 10.1017/S0950268807008424.View ArticlePubMedGoogle Scholar
- Paquet C, Quatresous I, Solet JL, Sissoko D, Renault P, Pierre V, Cordel H, Lassalle C, Thiria J, Zeller H, Schuffnecker I: Chikungunya outbreak in Reunion: epidemiology and surveillance, 2005 to early January 2006. Euro Surveill. 2006, 11 (2): E060202.3-PubMedGoogle Scholar
- Renault P, Solet J-L, Sissoko D, Balleydier E, Larrieu S, Filleul L, Lassalle C, Thiria J, Rachou E, De Valk H, Ilef D, Ledrans M, Quatresous I, Quenel P, Pierre V: A major epidemic of chikungunya virus infection in Reunion Island, France, 2005–2006. Am J Trop Med Hyg. 2007, 77 (4): 727-731.PubMedGoogle Scholar
- Institut National de Veille Sanitaire: Epidémie de Chikungunya à la Réunion/Océan Indien. Point au 17 février. 2006, [http://www.invs.sante.fr/presse/2006/le_point_sur/chikungunya_170206/index.html]Google Scholar
- Kish L: Some selection techniques. Survey sampling. 1965, Edited by Wiley and sons. New York, 440-501.Google Scholar
- Institut National de Veille Sanitaire: Epidémie de Chikungunya à la Réunion. Point de situation au 05 juillet 2006. [http://www.invs.sante.fr/presse/2006/le_point_sur/chikungunya_reunion_050706/chikungunya_reunion_s26.pdf]
- Grivard P, Le Roux K, Laurent P, Fianu A, Perrau J, Gigan J, Hoarau G, Grodin N, Staikowsky F, Favier F, Michault Al: Molecular and serological diagnosis of chikungunya virus infection. Pathol Biol (Paris). 2007 Oct 4,Google Scholar
- Gérardin P, Barau G, Michault A, Bintner M, Randrianaivo H, Choker G, Touret Y, Lenglet Y, Bouveret A, Grivard P, Le Roux K, Blanc S, Schuffenecker I, Couderc T, Arenzana-Seisdedos F, Lecuit M, Robillard PY: Multidisciplinary prospective study of mother-to-child chikungunya virus infections on the island of La Réunion. Plos Med. 2008, 5: e60-10.1371/journal.pmed.0050060. doi:10;1371/journal.pmed.0050060.View ArticlePubMedPubMed CentralGoogle Scholar
- Sissoko D, Malvy D, Giry C, Delmas G, Paquet C, Gabrie P, Pettineli F, Sanquer MA, Pierre V: Outbreak of chikungunya fever in Mayotte, Comoros archipelago, 2005–2006. Trans R Soc Trop Med Hyg. 2008 Apr 7,Google Scholar
- Institut National de Veille Sanitaire: Epidémie de chikungunya à la Réunion. Point de situation au 16 février 2006. [http://www.invs.sante.fr/presse/2006/le_point_sur/chikungunya_160206]
- Institut National de Veille Sanitaire: Epidémie de chikungunya à la Réunion. Point de situation au 23 février 2006. [http://www.invs.sante.fr/presse/2006/le_point_sur/chikungunya_230206]
- Ministère de la Santé et des Solidarités, Ministère de l'Outre Mer, Ministère délégué au tourisme: Plan global de lutte contre le chikunungya. [http://www.sante.gouv.fr/htm/actu/chikungunya/dossier_de_presse.pdf]
- Direction Régionale des Affaires Sanitaires et Sociales de la Réunion (DRASS) et Cellule interrégionale d'épidémiologie (CIRE) Réunion-Mayotte: Zoonoses. Chikungunya. Conseil aux voyageurs et réponses aux questions les plus fréquentes. [http://www.sante.gouv.fr/htm/pointsur/zoonose/12z_FAQ.htm]
- Blackburn N, Schoub B, O'Connell K: Reliability of the clinical surveillance criteria for measles diagnosis [letter]. Bull World Health Organ. 2000, 78: 861-PubMedPubMed CentralGoogle Scholar
- Kles V, Michault A, Rodhain F, Mevel F, Chastel C: Enquêtes sérologiques concernant les arboviroses à Flaviridae sur l'île de la Réunion (1971–1989). Bull Soc Pathol Exot. 1994, 87: 71-76.PubMedGoogle Scholar
- Thaikruea L, Chareansook O, Reanphumkamrit S, Dissomboon P, Phonian R, Ratchud S, Kounsang Y, Buranapiyawong D: Chikungunya in Thailand: a re-emerging disease?. 1997, 28: 359-364.Google Scholar
- Endy TP, Chunsuttiwat S, Nisalak A, Libraty DH, Green S, Rothman AL, Vaughn DW, Ennis FA: Epidemiology of inapparent and symptomatic acute dengue virus infection: a prospective study of primary school children in Kamphaeng, Thailand. Am J Epidemiol. 2002, 156: 40-51. 10.1093/aje/kwf005.View ArticlePubMedGoogle Scholar
- Méndez F, Barreto M, Arias JF, Rengifo G, Munoz J, Burbano ME, Parra B: Human and mosquito infections by dengue viruses during and after epidemics in a dengue-epidemic region of Colombia. Am J Trop Med Hyg. 2006, 74: 678-683.PubMedGoogle Scholar
- Biggerstaff BJ, Peterson LR: Estimated risk of West Nile Virus transmission through blood transfusion in the US, 2002. Transfusion. 2003, 43: 1007-1017. 10.1046/j.1537-2995.2003.00480.x.View ArticlePubMedGoogle Scholar
- CDC: Information and guidance for clinicians – West Nile virus: clinical description, 2004. [http://www.cdc.gov/ncidod/dvbid/westnile/clinicians/pdf/wnv-clinicaldescription.pdf]
- Sergon K, Njuguna C, Kalani R, Ofula V, Onyango C, Konongoi LS, Bedno S, Burke H, Dumila AM, Konde J, Njenga MK, Sang R, Breiman RF: Seroprevalence of chikungunya virus (CHIKV) infection on Lamu Island, Kenya, October 2004. Am J Trop Med Hyg. 2008, 78: 333-337.PubMedGoogle Scholar
- Sergon K, Yahaya AA, Brown J, Bedja SA, Mlindasse M, Agata N, Allaranger Y, Ball MD, Powers AM, Ofula V, Onyango C, Konongoi LS, Sang R, Njenga MK, Breiman RF: Seroprevalence of chikungunya virus infection on Grande Comore Island, Union of the Comoros, 2005. Am J Trop Med Hyg. 2007, 76: 1189-1193.PubMedGoogle Scholar
- Pialoux G, Gaüzere BA, Jaureguiberry S, Strobel S: Chikungunya, an epidemic arbovirosis. Lancet Infect Dis. 2007, 7: 319-327. 10.1016/S1473-3099(07)70107-X.View ArticlePubMedGoogle Scholar
- Boëlle PY, Thomas G, Vergu E, Renault P, Valleron AJ, Flahault A: Investigating transmission in a two-wave epidemic of chikungunya fever, Réunion Island. Vector Borne Zoonotic Dis. 2008, 8: 207-218. 10.1089/vbz.2006.0620.View ArticlePubMedGoogle Scholar
- Dumont Y, Chiroleu F, Domerq C: On a temporal model for the chikungunya disease: Modeling, theory and numerics. Math Biosci. 2008, 213: 80-91. 10.1016/j.mbs.2008.02.008.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/8/99/prepub
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