Muscular cystic hydatidosis: case report
© Vicidomini et al; licensee BioMed Central Ltd. 2007
Received: 16 August 2006
Accepted: 30 March 2007
Published: 30 March 2007
Hydatidosis is a zoonosis caused by Echinococcus granulosus, and ingesting eggs released through the faeces from infected dogs infects humans. The location of the hydatid cysts is mostly hepatic and/or pulmonary, whereas musculoskeletal hydatidosis is very rare.
We report an unusual case of primary muscular hydatidosis in proximity of the big adductor in a young Sicilian man. The patient, 34 years old, was admitted to the Department of Infectious and Tropical Diseases for ultrasonographic detection, with successive confirmation by magnetic resonance imaging, of an ovular mass (13 × 8 cm) in the big adductor of the left thigh, cyst-like, and containing several small cystic formations. Serological tests for hydatidosis gave negative results. A second drawing of blood was done 10 days after the first one and showed an increase in the antibody titer for hydatidosis. The patient was submitted to surgical excision of the lesion with perioperatory prophylaxis with albendazole. The histopathological examination of the bioptic material was not diriment in the diagnosis, therefore further tests were performed: additional serological tests for hydatidosis for the evaluation of IgE and IgG serotype (Western Blot and REAST), and molecular analysis of the excised material. These more specific serological tests gave positive results for hydatidosis, and the sequencing of the polymerase chain reaction products from the cyst evidenced E. granulosus DNA, genotype G1. Any post-surgery complications was observed during 6 following months.
Cystic hydatidosis should always be considered in the differential diagnosis of any cystic mass, regardless of its location, also in epidemiological contests less suggestive of the disease. The diagnosis should be achieved by taking into consideration the clinical aspects, the epidemiology of the disease, the imaging and immunological tests but, as demonstrated in this case, without neglecting the numerous possibilities offered by new serological devices and modern day molecular biology techniques.
Hydatidosis is a zoonotic infection caused by tapeworms belonging to the class Cestoda, in the family Taeniidae, of the genus Echinococcus. The Echinococcus granulosus species, which is responsible for cystic hydatidosis, has an almost ubiquitous diffusion. South America, Central Asia and the Mediterranean basin  must be considered highly endemic areas. The incidence of the disease in Italy is rated high in Sardinia and Sicily, medium in the Central-South regions, and low in the remaining areas . The adult worm (3 to 6 mm long) lives in the small intestine of the definitive hosts, i.e. dogs or other canids . Gravid proglottids containing infective eggs are shed daily through the faeces. After ingestion by a suitable intermediate host (usually herbivores like sheep, goats, swine, cattle, horses, camels, but occasionally also humans), the eggs hatch in the small intestine releasing a hooked larva called oncosphere. It penetrates the intestinal wall by means of its six hooks and migrates through the circulatory system reaching various organs, mainly the liver and lungs. Here the oncosphere loses the hooks and develops into a cyst that enlarges gradually. Usually by the fifth month, the wall of the cyst differentiates into an outer laminated non-nucleated layer and an inner nucleated germinal layer. The inner layer produces protoscolices and daughter cysts that fill the cyst interior, and can be attached or floating free within the cyst fluid. The dog becomes infected after swallowing the cyst-containing organs of the slaughtered parasitized herbivores. The ingested protoscolices attach to the intestinal mucosa, and develop into adult stage tapeworms within 32–80 days. Humans are accidental hosts that become infected by ingesting the eggs and, just like the aforementioned herbivorous hosts, allow the development of cysts in various organs. The growth rate of the cysts is about 1 cm per year. The size of the cysts varies between 1 and 15 cm, even though descriptions of cysts of up to 20 cm in diameter can be found in literature . Cysts are typically univesicular, but sometimes small daughter cysts similar to the mother cyst, can be found in their interior. Hydatids are mostly found in the liver and/or in lungs for physiopathological reasons, but several other encysting sites are possible, bone included. The musculoskeletal involvement has been registered in only 1–4% of the cases . It has been hypothesized that the presence of lactic acid in the muscles does not allow the larvae to grow into cysts . Nevertheless, some cases of primary muscular hydatidosis at various sites have been reported, i.e. thoracic wall , sartorius [6, 7], biceps brachii , supraspinatus , gluteus , pterygoideus  and soleus muscles , whereas, only few cases of primary subcutaneous hydatidosis of the inferior limbs have been reported [12–15]. In this study we report a case of muscular cystic hydatidosis of the adductor magnus muscle in a young Italian man from Sicily. We would like to point out that, regardless of the site involved, this zoonotic infection should always be considered in the differential diagnosis of any cystic lesion. Furthermore, it is extremely important to use all the available diagnostic methodologies to reach a definitive diagnosis.
Hydatidosis is a serious public health problem in some areas of the World. Clandestine slaughtering together with climatic and ecological conditions, favour the spreading of the parasite among animals and contribute to maintain high infection rates in humans. Unfortunately, this practice is still very common in some areas of Italy despite the more severe regulations, which were adopted after the cases of human bovine spongiform encephalopathy (BSE). Early diagnosis of this zoonosis can be difficult, even in the more common hepatic and/or pulmonary cysts. The identification actually becomes more complex when the cysts are located in unusual sites, such as the musculoskeletal hydatidosis, and the parasitic aetiology is therefore often unsuspected. Hydatidosis should always be considered when a patient coming from an endemic area, shows a slow growing mass in the soft tissues. In our case, although we had diagnostic images highly suggestive of cystic hydatidosis, we did not have any other elements to support this hypothesis. As a matter of fact, even though our patient was originally from Sicily, an Italian region considered a high incidence area for hydatidosis; he had been living in Tuscany for the past 10 years, a middle incidence area for the disease. The anamnesis was negative for direct contact with dogs or stock farm environment. The patient had noticed a rapid (one month) increase in size of the swelling of the thigh. Imaging showed no evidence of lesions in the liver and lungs. Initial serologic screening tests, IHA and IFA, were negative. Surgical excision of the lesion was prompted by the need of reaching a definitive diagnosis, under perioperative chemoprophylaxis with albendazole. The patient had no post-surgery complications. Serological tests performed at the end of the perioperative prophylaxis and repeated two months later resulted negative. Microscopic and histopathologic findings showed the presence of some morphological features of a parasitic cyst, but it was not conclusive in confirming the presumptive diagnosis. Molecular testing of the surgical specimen allowed us to identify E. granulosus DNA, genotype G1, in the nucleated inner layer of the cyst wall. The experience here reported suggests that cystic hydatidosis should always be considered in the differential diagnosis of any cystic mass, regardless of its location, and of the epidemiological contexts which might be less suggestive of the disease. The diagnosis, as confirmed by this case, should be made by taking into account the clinical aspects and imaging analysis, but also the serological tests. IHA and IFA confirmed to be suboptimal tests, especially in extra-hepatic localizations, whereas WB which is known to be the most specific assay for E. granulosus, showed higher sensitivity. Furthermore, the additional use of molecular diagnostics may be a helpful tool to validate an indirect (presumptive) diagnosis. In this case, we identified a strain of E. granulosus, genotype G1, which is commonly found in sheep . These biomolecular findings allowed us to establish that sheep farming environment or contaminated foods coming from the same area were our patient's source of infection. In addition, this diagnostic tool allowed us to exclude areas where cattle, swine, horses or goats were bred.
"Written consent was obtained from the patient for publication of the patient's details."
We would like to thank Mrs. Ornella Merolla, a native language speaker, whose knowledge of the English language and expertise with medical terms was very helpful in editing this paper.
- Eckert J, Deplazes P: Biological, Epidemiological and Clinical Aspects of Echinococcosis, a Zoonosis of Increasing Concern. Clin Microbiol Rev. 2004, 17: 1, 107-135. 10.1128/CMR.17.1.107-135.2004.View ArticleGoogle Scholar
- Garippa G: Updates on cystic echinococcosis (CE) in Italy. Parassitologia. 2006, 48: 57-59.PubMedGoogle Scholar
- Merkle EM, Schulte M, Vogel J, Tomczak R, Rieber A, Kern P, Goerich J, Brambs HJ, Sokiranski R: Musculoskeletal involvement in cystic echinococcosis: report of eight cases and review of the literature. AJR Am J Roentgenol. 1997, 168: 1531-4.View ArticlePubMedGoogle Scholar
- Duncan GJ, Tooke SM: Echinococcus infestation of the biceps brachii. A case report. Clin Orthop Relat Res. 1990, 261: 247-50.PubMedGoogle Scholar
- Alvarez-Sala R, Caballero P: Echinococcus cyst as a cause of chest wall tumor. Ann Thorac Surg. 1987, 43: 689-690.View ArticlePubMedGoogle Scholar
- Rask MR, Lattig GJ: Primary intramuscular hydatidosis of the sartorius. J Bone Joint Surg Am. 1970, 52: 582-584.PubMedGoogle Scholar
- Duygulu F, Karaoglu S, Erdogan N, Yildiz O: Primary hydatid cyst of the thigh: a case report of an unusual localization. Turk J Pediatr. 2006, 48 (3): 256-9.PubMedGoogle Scholar
- Tatari H, Baran O, Sanlidag T, Gore O, Ak D, Manisali M, Havitcioglu H: Primary intramuscular hydatidosis of supraspinatus muscle. Arch Orthop Trauma Surg. 2001, 121: 93-94. 10.1007/PL00013775.View ArticlePubMedGoogle Scholar
- Combalia A, Sastre-Solsona S: Hydatid cyst of gluteus muscle. Two cases. Review of the literature. Joint Bone Spine. 2005, 72 (5): 430-2. 10.1016/j.jbspin.2004.11.007. Epub 2005 Aug 2View ArticlePubMedGoogle Scholar
- Turki I, Turki A, Khochtali H, Bakir D, Bakir A: Pterygoidien hydatid cyst. Rev Stomatol Chir Maxillofac. 2005, 106: 27-9.View ArticlePubMedGoogle Scholar
- Togrul E, Kalaci A, Sarpel Y, Koltay IS, Ozbarlas S: What's your diagnosis? Solitary hydatid cyst of the soleus muscle. Ann Saudi Med. 2004, 24: 288, 307-9.Google Scholar
- Chevalier X, Rhamouni A, Bretagne S, Martigny J, Piet BL: Hydatid Cyst of the Subcutaneous Tissue Without Other Involvement: MR Imaging Features. AJR. 1994, 163: 645-646.View ArticlePubMedGoogle Scholar
- Memis A, Arkun R, Bilgen I, Uston EE: Primary soft tissue hydatid disease: report of two cases with MRI characteristics. Eur Radiol. 1999, 9: 1101-1103. 10.1007/s003300050798.View ArticlePubMedGoogle Scholar
- Orhan Z, Kara H, Tuzuner T, Sencan I, Alper M: Primary subcutaneous cyst hydatic disease in proximal thigh: an unusual localisation: a case report. BMC Musculoskelet Disor. 2003, 7: 4-25.Google Scholar
- Kazakos CJ, Galanis VG, Verettas DA, Polychronidis A, Simopoulos C: Primary hydatid disease in femoral muscles. J Int Med Res. 2005, 33: 703-6.View ArticlePubMedGoogle Scholar
- Dinkel A, Von nickisch-rosenegk M, Bilger B, Merli M, 1 Lucius R, Thomas Romig T: Detection of Echinococcus multilocularis in the Definitive Host: Coprodiagnosis by PCR as an Alternative to Necropsy. J Clin Microbiol. 1998, 36: 1871-1876.PubMedPubMed CentralGoogle Scholar
- Kumar S, Tamura K, Nei M: MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinfor. 2004, 5: 150-63. 10.1093/bib/5.2.150.View ArticleGoogle Scholar
- Thompson RCA, McManus DP: Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002, 18: 452-57. 10.1016/S1471-4922(02)02358-9.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/7/23/prepub
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