Figure 1From: Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva Structure of Real time PCR design for Actinobacillus actinomycetemcomitans 652, JP2 genotype detection. Primers were designed to circumscribe a fragment of 530 bp present in 652 but deleted in the JP2 strain. The two different PCR products showed 6 °C difference in Tms after PCR real time melting curve analysis.Back to article page