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Extended Spectrum β-Lactamases among Gram-negative bacteria of nosocomial origin from an Intensive Care Unit of a tertiary health facility in Tanzania
© Ndugulile et al; licensee BioMed Central Ltd. 2005
Received: 27 May 2005
Accepted: 15 October 2005
Published: 15 October 2005
Resistance to third generation cephalosporins due to acquisition and expression of extended spectrum β-lactamase (ESBL) enzymes among Gram-negative bacteria is on the increase. Presence of ESBL producing organisms has been reported to significantly affect the course and outcome of an infection. Therefore infections due to ESBL isolates continue to pose a challenge to infection management worldwide. The aim of this study was to determine the existence and to describe phenotypic and genotypic characteristics of ESBLs in an Intensive Care Unit (ICU) setting in Tanzania.
Between October 2002 and April 2003, clinical information and samples were collected from patients suspected to have nosocomial infections in an Intensive Care Unit of a tertiary hospital in Tanzania. The isolates were identified, tested for antimicrobial susceptibility and analysed for presence of ESBL genes.
Thirty-nine Gram-negative bacteria were isolated from clinical samples of 39 patients. These isolates included 13 Escherichia coli, 12 Enterobacter spp, 5 Pseudomonas spp, 4 Proteus spp, 2 Klebsiella. pneumoniae, 2 Citrobacter freundii and 1 Chryseomonas luteola. Eleven (28.2%) of these isolates were ESBL producing. The ESBL genes characterised were SHV-12, SHV-28 and CTX-M-15. The ESBL producing isolates were more resistant to gentamicin and ciprofloxacin than non-ESBL producing isolates.
This study shows the presence of ESBL genes among Gram-negative bacteria in the ICU setting in Tanzania. There is a need to institute strict hospital infection control policy and a regular surveillance of resistance to antimicrobial agents.
Extended spectrum β-lactamase (ESBL) enzymes have been reported in a number of species in Gram-negative bacteria. The ESBL enzymes are usually plasmid mediated and are capable of hydrolysing and inactivating a wide variety of β-lactams, including third-generation cephalosporins, penicillins and aztreonam, but are susceptible to β-lactamase inhibitors such as clavulanate, sulbactam and tazobactam [1, 2]. Many ESBL producers also carry other genes that confer resistance to other antimicrobial agents such as aminoglycosides and fluoroquinolones [3, 4].
Extensive use of broad spectrum antibiotics, prolonged hospitalisation, indwelling devices and severe underlying diseases have been reported as factors that have led to spread of ESBL and difficulties in managing severe infections in many parts of the world [5, 6].
Treatment of ESBL-producing bacterial infections can place an added constraint on already overburden health systems in developing countries. ESBL producing organisms are reported to account for a significant proportion of intensive care infections . Problems of ESBLs have led to limited, as well as expensive treatment options, and have impacted negatively on clinical outcomes . Nosocomial infections due to ESBL producing organisms have been known to cause high mortality .
Very few studies have reported on the problem of ESBLs in Africa in general and Tanzania in particular. There have been reports of CTX -M-12 ESBL in Klebsiella pneumoniae in Kenya , TEM-53, TEM-63, SHV-2, SHV-5, SHV-19, SHV-20, SHV-21 and SHV-22 in K. pneumoniae in South Africa , CTX-M 15 and SHV-12 in Tanzania , SHV-12 in Salmonella enterica serotype Babelsberg and Enteretidis from Mali orphans  and TEM-3 in S. typhimurium in Morocco .
The aim of this study was to determine the existence and to describe phenotypic and genotypic characteristics of ESBLs in an Intensive Care Unit (ICU) setting in Tanzania.
Muhimbili National Hospital is a 1000 bed tertiary health facility with an eight-bed ICU that caters for surgical, medical and trauma emergencies. During the study period October 2002 to April 2003, 206 patients were admitted to the ICU and of these 50 specimens were collected from patients with suspected nosocomial infections, according to definitions as described by the Centers for Disease Control (CDC) . In particular, infections were considered to be nosocomial if symptoms and signs appeared >48 hours following admission to the ICU.
Bacterial strains and susceptibility testing
Specimen from 50 patients with suspected nosocomial infections were collected and cultured on blood agar (Oxoid Ltd, Basingstoke, UK) and MacConkey agar (Difco/BD Diagnostics Systems, Sparks, MI, USA) except for urine samples which were plated on Cysteine Lactose Electrolytes Deficient (CLED) agar. Isolated strains were stored in tubes containing 1.5 ml Brain Heart Infusion broth with 10% v/v glycerol at -70°C until the time of analysis. The isolates were identified using biochemical tests and confirmed using the API 20E and API 20 NE identification systems (bioMerieux, Marcy-l'Etoile, France).
The susceptibility of the Enterobacteriaceae to antimicrobial agents was examined by an agar diffusion method using paper disks (AB Biodisk, Solna, Sweden) containing the following antibiotic concentration : amoxicillin/clavulanic acid (20/10 μg), ampicillin (10 μg), ceftriaxone (30 μg), cefuroxime (30 μg), chloramphenicol (30 μg), ciprofloxacin (5 μg), doxycycline (30 μg), gentamicin (10 μg) and trimethoprim/sulfamethoxazole (1.25/23.75 μg). The susceptibility of the non-Enterobacteriaceae was examined using ceftriaxone, ciprofloxacin, ceftazidime (30 μg), gentamicin, imipenem (10 μg) and tobramycin (10 μg). E. coli ATCC 25922 and P. aeruginosa ATCC 27853 were used as quality control strains.
Testing for presence of ESBL
Gram negative bacteria with reduced susceptibility to third generation cephalosporins according to the NCCLS criteria , that is, zones of inhibition of = 27 mm for cefotaxime and = 22 mm for ceftazidime, were tested by three ESBL Etest strips (AB Biodisk, Solna, Sweden) on Mueller Hinton agar. Minimum Inhibitory Concentrations of cefotaxime (CT), ceftazidime (TZ) and cefepime (PM) with and without clavulanic acid were used. The inoculated plates were incubated for 16–18 hours at 37°C.
ESBL results were read either as MIC values or observation of 'phantom' zones or deformation of inhibition ellipses. Reduction of MIC by = 3 two-fold dilutions in the presence of clavulanic acid was indicative of ESBL production. Deformation of ellipses or the presence of a 'phantom' zone was also indicative of ESBL production even if the MIC ratio is <8 or cannot be read. Isolates were reported as having ESBL phenotype if one or more of the three ESBL Etests were positive.
The outcome of the test was indeterminate when MICs were outside the test range of the test device. Those strains found to be ESBL producing phenotypically by Etest were examined for the presence of the TEM, SHV and CTX-M genes by Polymerase Chain Reaction (PCR).
Molecular analysis techniques
Isolates with ESBL phenotype were examined for the presence of bla TEM, bla SHV and blaCTX-M by PCR [17, 18]. The PCR products were purified using QIAquick PCR Purification Kits (Qiagen, Hilden, Germany). The templates were sequenced using the same primers as used in the PCR amplification. The cycle sequencing parameters were 25 cycles at 96°C for 10 seconds, 58°C for 5 seconds (50°C for bla CTX-M) and 60°C for 4 minutes. The products were analysed using an ABI PRISM 3700 DNA sequencer (PE Biosystems, Warington, UK). Point mutations were accepted if present in both the forward and reverse sequences.
Analysis of chromosomal DNA by PFGE
Pulsed-field gel electrophoresis (PFGE) was performed as described previously by Gautom et al . DNA of all isolates was digested using XbaI (New England BioLabs, Beverly, Mass., USA) at 37°C for 4 hours, according to supplier's instructions. The slices of the digested DNA were loaded into the wells of the poured gels. Electrophoresis agarose gel (Promega, Madison, USA) with a concentration of 1% was used for different organisms in 0.5 × Tris-Borate -EDTA buffer using contour -clamped homogenous electric field apparatus (CHEF-DR III; Bio-Rad, Richmond, Calif., USA). A 48.5 kb lambda PFG marker 50 ug/ml (New England BioLabs) was used as a marker. The conditions for electrophoresis were angle, 120°; gradient, 6 V/cm; temperature, 14°C; pulse times ranging from 10 to 45 s and running time was 20 hours. The fragment patterns were interpreted as described by Tenover et al .
All statistics were performed by SPSS software 11.5. Contingency table analysis was done by χ2 test or two-tailed Fisher's exact test for categorical variables. P-value of <0.05 was considered statistically significant.
Bacteriology and antimicrobial susceptibility pattern
A total of 50 bacteria were isolated from 50 patients suspected of having nosocomial infections. These consisted of 30 Urinary Tract Infections, 15 wound infections, 3 blood stream infections and 2 cases of pneumonia. Out of these 50 bacterial isolates, 39 were Gram-negative bacteria, The remaining eleven isolates were S. aureus. These Gram-negative isolates were identified as Escherichia coli (N = 13), Enterobacter cloacae (N = 8), Enterobacter aerogenes (N = 3), Pantoea agglomerans (N = 1), Pseudomonas aeruginosa (N = 5), Proteus mirabilis (N = 3), Proteus vulgaris (N = 1), Klebsiella pneumoniae (N = 2), Citrobacter freundii (N = 2) and Chryseomonas luteola (N = 1).
Antimicrobial susceptibility pattern of ESBL and non-ESBL producing isolates from the ICU, Dar es Salaam, Tanzania§.
ESBL (n = 11)
Non-ESBL (n = 22)
PCR and sequencing of ESBL genes
Characteristics of ESBL producing isolates from ICU, Dar es Salaam, Tanzania.
Date of Isolation
13 December 2002
27 October 2002
30 December 2002
16 February 2002
18 October 2002
22 October 2002
28 November 2002
05 December 2002
30 November 2002
30 December 2002
20 February 2003
Nucleotide sequence analysis of the isolates carrying bla CTX-M revealed that all isolates carried the CTX-M-15 gene. Of the seven isolates that had bla SHV, six had SHV-12 and one had SHV-28 (Table 2). TEM-1 was identified in TEM producing isolates.
Genotypic relationship of the ESBL isolates
Two of the 13 E. coli isolated had indistinguishable restriction patterns on PFGE. These were isolates from two different patients and from different specimen (urine and pus). The two patients had at one time been together in the ICU. Other isolates tested were unrelated.
This study demonstrates the presence of ESBL-mediated resistance in bacteria causing infections in the ICU of a tertiary hospital in Tanzania. Despite the rise in the prevalence of ESBL in some countries, there are very few reports from Africa [10–12, 14]. ESBL detection is not commonly carried out in many microbiology units in developing countries, Tanzania included. This could be attributed to lack of awareness and lack of resources and facilities to conduct ESBL identification. The high rate of resistance noted among the isolates in the present study, although few in numbers, is of serious concern. Eleven of the 39 (28.2%) of the Enterobacteriaceae were ESBL producing.
In this study, ESBL producing isolates were significantly more resistant to ciprofloxacin (p = 0.017), ceftriaxone (p = 0.000) and gentamicin (p = 0.003) as compared to non-ESBL producing Gram-negative isolates. Other studies have reported on cross-resistance to aminoglycosides, fluoroquinolones and trimethoprim/sulfamethoxazole in ESBL producing organisms [21, 22]. Mechanisms of co-resistance are not clear, but one possible mechanism is the co-transmission of ESBL and resistance to other antimicrobials within the same conjugative plasmids .
This study reports on the presence of CTX-M-15 producing isolates in the ICU setting in Tanzania. CTX-M-15 gene was also found among bloodstream infection isolates from children within the same hospital . This gene is widespread in European and Asian countries including Northern Italy , United Kingdom  and India . The other reported gene is CTX-M-12 that was identified amongst K. pneumoniae isolates from cerebrospinal fluid and blood in Kenya . In the present study, the CTX-M-15 gene was found in two different genera of the bacterial isolates in the ICU (E. coli and E. cloacae). Two of the CTX-M-15 producing isolates were also found to possess the SHV-12 gene. The two E. coli with indistinguishable PGFE restriction patterns were found to be resistant to all antimicrobials tested except for chloramphenicol.
SHV-12 was found in the two strains of E. coli with indistinguishable PFGE restriction patterns. SHV-12 was also found among bloodstream infection isolates from children within the same hospital . This gene has also been reported in several European and Asian countries including Switzerland  and Thailand .
In the present study, this gene was found across different genera of bacterial isolates. The other SHV related genes that have been reported in Africa include SHV-2, SHV-5, SHV-19, SHV-20, SHV-21 and SHV-22 that were reported in South Africa .
In this study, one K. pneumoniae isolate was found to possess SHV-28. Initial reports of this gene came from China (GenBank accession no. AF299299). To the best of our knowledge, there are no other reports of this gene in the literature. This isolate was obtained from a urine specimen from a patient with urinary tract infection. This isolate was resistant to amoxicillin/clavulanic acid, doxycycline and trimethoprim/sulfamethoxazole and cephalosporins but sensitive to chloramphenicol, ciprofloxacin and gentamicin.
Presence of similar SHV, TEM and CTX-M genes in various non-genetically related strains suggests a possibility of horizontal transfer of these genes in the ICU. On the other hand, the two E. coli that produced indistinguishable restriction patterns on PFGE suggests that clonal spread of the resistant bacteria is also a potential factor in the spread of resistance in the ICU. These finding of similar ESBL genes in the ICU and in paediatric wards  within the same hospital could mean the ESBL genes are widespread in this hospital.
This study shows that there is a presence of ESBL producing isolates in the ICU of a major hospital in Tanzania. This is also the first report of presence of SHV-28 in Africa. This study also shows that the ESBL phenotype spread in this hospital is due to multiple enzymes found in different genera of bacteria. There is a need to institute a strict hospital infection control policy and a regular surveillance of resistance to antimicrobial agents.
We would like to thank the staff at the ICU in Dar es Salaam for their assistance and to Prof Victor Mwafongo for his advice and assistance in Dar es Salaam, Tanzania. We would also like to thank Dr Bjorn Blomberg for reviewing the manuscript and for the provision of laboratory assistance in Bergen, Johanna Sollid and Mekonnen Kurabachew for their assistance in interpreting sequence results and to Marit Gjerde Tellevik for her assistance with the laboratory methods and supplies. This study was supported by the grant from the Norwegian State Education Loan Fund.
- Sturenburg E, Mack D: Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control. J Infect. 2003, 47: 273-295. 10.1016/S0163-4453(03)00096-3.View ArticlePubMedGoogle Scholar
- Gniadkowski M: Evolution and epidemiology of extended-spectrum beta-lactamases (ESBLs) and ESBL-producing microorganisms. Clin Microbiol Infect. 2001, 7: 597-608. 10.1046/j.1198-743x.2001.00330.x.View ArticlePubMedGoogle Scholar
- Spanu T, Luzzaro F, Perilli M, Amicosante G, Toniolo A, Fadda G: Occurrence of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae in Italy: implications for resistance to beta-lactams and other antimicrobial drugs. Antimicrob Agents Chemother. 2002, 46: 196-202. 10.1128/AAC.46.1.196-202.2002.View ArticlePubMedPubMed CentralGoogle Scholar
- Sekowska A, Janicka G, Klyszejko C, Wojda M, Wroblewski M, Szymankiewicz M: Resistance of Klebsiella pneumoniae strains producing and not producing ESBL (extended-spectrum beta-lactamase) type enzymes to selected non-beta-lactam antibiotics. Med Sci Monit. 2002, 8: BR100-4.PubMedGoogle Scholar
- Nathisuwan S, Burgess DS, Lewis JS: Extended-spectrum beta-lactamases: epidemiology, detection, and treatment. Pharmacotherapy. 2001, 21: 920-928. 10.1592/phco.21.11.920.34529.View ArticlePubMedGoogle Scholar
- Lin MF, Huang ML, Lai SH: Risk factors in the acquisition of extended-spectrum beta-lactamase Klebsiella pneumoniae: a case-control study in a district teaching hospital in Taiwan. J Hosp Infect. 2003, 53: 39-45. 10.1053/jhin.2002.1331.View ArticlePubMedGoogle Scholar
- Paterson DL, Ko WC, Von Gottberg A, Mohapatra S, Casellas JM, Goossens H, Mulazimoglu L, Trenholme G, Klugman KP, Bonomo RA, Rice LB, Wagener MM, McCormack JG, Yu VL: International prospective study of Klebsiella pneumoniae bacteremia: implications of extended-spectrum beta-lactamase production in nosocomial Infections. Ann Intern Med. 2004, 140: 26-32.View ArticlePubMedGoogle Scholar
- Menashe G BA, Yagupsky P, Peled N, Gilad J, Fraser D, Riesenberg K, Schlaeffer F: Clinical significance and impact on mortality of extended-spectrum beta lactamase-producing Enterobacteriaceae isolates in nosocomial bacteremia. Scand J Infect Dis. 2001, 33: 188-193. 10.1080/00365540151060806.View ArticlePubMedGoogle Scholar
- Ho PL, Chan WM, Tsang KW, Wong SS, Young K: Bacteremia caused by Escherichia coli producing extended-spectrum beta-lactamase: a case-control study of risk factors and outcomes. Scand J Infect Dis. 2002, 34: 567-573. 10.1080/00365540210147516.View ArticlePubMedGoogle Scholar
- Kariuki S, Corkill JE, Revathi G, Musoke R, Hart CA: Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya. Antimicrob Agents Chemother. 2001, 45: 2141-2143. 10.1128/AAC.45.7.2141-2143.2001.View ArticlePubMedPubMed CentralGoogle Scholar
- Essack SY, Hall LM, Pillay DG, McFadyen ML, Livermore DM: Complexity and diversity of Klebsiella pneumoniae strains with extended-spectrum beta-lactamases isolated in 1994 and 1996 at a teaching hospital in Durban, South Africa. Antimicrob Agents Chemother. 2001, 45: 88-95. 10.1128/AAC.45.1.88-95.2001.View ArticlePubMedPubMed CentralGoogle Scholar
- Blomberg B, Jureen R, Manji KP, Tamim BS, Mwakagile DS, Urassa WK, Fataki M, Msangi V, Tellevik MG, Maselle SY, Langeland N: High rate of fatal cases of pediatric septicemia caused by gram-negative bacteria with extended-spectrum beta-lactamases in Dar es Salaam, Tanzania. J Clin Microbiol. 2005, 43: 745-749. 10.1128/JCM.43.2.745-749.2005.View ArticlePubMedPubMed CentralGoogle Scholar
- Weill FX, Demartin M, Tande D, Espie E, Rakotoarivony I, Grimont PA: SHV-12-like extended-spectrum-beta-lactamase-producing strains of Salmonella enterica serotypes Babelsberg and Enteritidis isolated in France among infants adopted from Mali. J Clin Microbiol. 2004, 42: 2432-2437. 10.1128/JCM.42.6.2432-2437.2004.View ArticlePubMedPubMed CentralGoogle Scholar
- AitMhand R, Soukri A, Moustaoui N, Amarouch H, ElMdaghri N, Sirot D, Benbachir M: Plasmid-mediated TEM-3 extended-spectrum beta-lactamase production in Salmonella typhimurium in Casablanca. J Antimicrob Chemother. 2002, 49: 169-172. 10.1093/jac/49.1.169.View ArticlePubMedGoogle Scholar
- Garner JS JW, Emori TG, Horan TC, Hughes JM: CDC definitions for nosocomial infections. Infection Control and Applied Epidemiology:Principles and Practice. Edited by: RN O. 1996, St. Louis, Mosby, A-1-A-20.Google Scholar
- National Committee for Clinical Laboratory Standards: Performance standards for antimicrobial susceptibility testing; ninth informational supplement. M100-S9. 1999, Wayne, PA,Google Scholar
- Rasheed JK, Jay C, Metchock B, Berkowitz F, Weigel L, Crellin J, Steward C, Hill B, Medeiros AA, Tenover FC: Evolution of extended-spectrum beta-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia. Antimicrob Agents Chemother. 1997, 41: 647-653.PubMedPubMed CentralGoogle Scholar
- Pagani L, Dell'Amico E, Migliavacca R, D'Andrea MM, Giacobone E, Amicosante G, Romero E, Rossolini GM: Multiple CTX-M-type extended-spectrum beta-lactamases in nosocomial isolates of Enterobacteriaceae from a hospital in northern Italy. J Clin Microbiol. 2003, 41: 4264-4269. 10.1128/JCM.41.9.4264-4269.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Gautom RK: Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. J Clin Microbiol. 1997, 35: 2977-2980.PubMedPubMed CentralGoogle Scholar
- Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995, 33: 2233-2239.PubMedPubMed CentralGoogle Scholar
- Alhambra A, Cuadros JA, Cacho J, Gomez-Garces JL, Alos JI: In vitro susceptibility of recent antibiotic-resistant urinary pathogens to ertapenem and 12 other antibiotics. J Antimicrob Chemother. 2004, 53: 1090-1094. 10.1093/jac/dkh218.View ArticlePubMedGoogle Scholar
- Procop GW, Tuohy MJ, Wilson DA, Williams D, Hadziyannis E, Hall GS: Cross-class resistance to non-beta-lactam antimicrobials in extended-spectrum beta-lactamase-producing Klebsiella pneumoniae. Am J Clin Pathol. 2003, 120: 265-267. 10.1309/BWQK-WB2W-N6W5-X1CC.View ArticlePubMedGoogle Scholar
- Martinez-Martinez L, Pascual A, Jacoby GA: Quinolone resistance from a transferable plasmid. Lancet. 1998, 351: 797-799. 10.1016/S0140-6736(97)07322-4.View ArticlePubMedGoogle Scholar
- Mushtaq S, Woodford N, Potz N, Livermore DM: Detection of CTX-M-15 extended-spectrum beta-lactamase in the United Kingdom. J Antimicrob Chemother. 2003, 52: 528-529. 10.1093/jac/dkg353.View ArticlePubMedGoogle Scholar
- Karim A, Poirel L, Nagarajan S, Nordmann P: Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett. 2001, 201: 237-241. 10.1016/S0378-1097(01)00276-2.PubMedGoogle Scholar
- Nuesch-Inderbinen MT, Kayser FH, Hachler H: Survey and molecular genetics of SHV beta-lactamases in Enterobacteriaceae in Switzerland: two novel enzymes, SHV-11 and SHV-12. Antimicrob Agents Chemother. 1997, 41: 943-949.PubMedPubMed CentralGoogle Scholar
- Chanawong A, M'Zali FH, Heritage J, Lulitanond A, Hawkey PM: SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum beta-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand. J Antimicrob Chemother. 2001, 48: 839-852. 10.1093/jac/48.6.839.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/5/86/prepub
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