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BMC Infectious Diseases

Open Access

Resistance profile of Staphylococcus aureusstrains isolated from patients treated in a tertiary care hospital in Romania

  • Oana Săndulescu1, 2Email author,
  • Andrei Grigoraş1,
  • Anca Streinu-Cercel1, 2,
  • Ioana Berciu1, 2,
  • Alina Cristina Neguț1, 2 and
  • Adrian Streinu-Cercel1, 2
BMC Infectious Diseases201414(Suppl 7):P59

https://doi.org/10.1186/1471-2334-14-S7-P59

Published: 15 October 2014

Background

The prevalence of colonization with Staphylococcus aureus has been shown to increase worldwide [1, 2], along with the rate of antibiotic use in medicine as well as the livestock industry. Therefore, our microbiota may be exposed to selective pressure and may develop resistance to current drugs [3].

Methods

We have performed a study to assess the antimicrobial susceptibility profile of S. aureus strains isolated from clinically apparent infections treated in the National Institute for Infectious Diseases "Prof. Dr. Matei Balş". Bacterial identification and antimicrobial sensitivity testing were performed on MALDI-TOF or VITEK (bioMérieux, Paris, France).

Results

We examined 149 strains of S. aureus, 44.9% from cutaneous wound infections, 31.2% from blood cultures, 8.4% from sputum samples and 15.3% from other infection sites. Of the total number of strains identified, 55.7% were resistant to methicillin, 35.1% were resistant to clindamycin (D-test results are presented separately), 30.9% were resistant to levofloxacin, 18.7% were resistant to rifampin and smaller percentages were identified for resistance to other drugs. Surprisingly, resistance was also identified to drugs that are not used in clinical practice in Romania, such as daptomycin (6.8%) or fusidic acid (4.8%), suggesting a possible international circulation of S. aureus strains, probably through means of nasal or axillary carriage. We excluded a mechanism of daptomycin non-susceptibility through thickened bacterial cell walls in strains of vancomycin-intermediate S. aureus, as all strains were susceptible to vancomycin.

Conclusion

Antimicrobial resistance remains a major issue in clinical practice, but studies describing the local antimicrobial susceptibility patterns of important pathogens can aid in guiding first-line antibiotherapy.

Declarations

Acknowledgements

1) This paper is partially supported by the Sectoral Operational Programme Human Resources Development (SOP HRD), financed from the European Social Fund and by the Romanian Government under the contract numbers POSDRU/159/1.5/S/137390.

2) This paper is partially supported by the Carol Davila University of Medicine and Pharmacy, Young Researchers Projects Grant Competition, contract number 28.336/04.11.2013.

Authors’ Affiliations

(1)
Carol Davila University of Medicine and Pharmacy
(2)
National Institute for Infectious Diseases "Prof. Dr. Matei Balş"

References

  1. Preoțescu LL, Streinu-Cercel O: Prevalence of nasal carriage of S aureus in children. GERMS. 2013, 3: 49-51. 10.11599/germs.2013.1035.View ArticleGoogle Scholar
  2. Mimica MJ, Bádue-Pereira MF: Staphylococcus aureus colonization in Brazilian children. GERMS. 2014, 4: 22-10.11599/germs.2014.1051.PubMed CentralView ArticlePubMedGoogle Scholar
  3. de Lastours V, Chau F, Roy C, Larroque B, Fantin B: Emergence of quinolone resistance in the microbiota of hospitalized patients treated or not with a fluoroquinolone. J Antimicrob Chemother. 2014, pii: dku283-Google Scholar

Copyright

© Săndulescu et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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