Volume 14 Supplement 7

Proceedings of the 10th Edition of the Scientific Days of the National Institute for Infectious Diseases "Prof Dr Matei Bals"

Open Access

Nasopharyngeal microbiota evaluation in three cohorts of children in the Romanian pediatric population

  • Anca Streinu-Cercel1, 2Email author,
  • Oana Săndulescu1, 2,
  • Ioana Berciu1, 2,
  • Alina Cristina Neguț1, 2,
  • Mihai Săndulescu1, 3,
  • Ruxandra Aursulesei1 and
  • Adrian Streinu-Cercel1, 2
BMC Infectious Diseases201414(Suppl 7):O21

https://doi.org/10.1186/1471-2334-14-S7-O21

Published: 15 October 2014

Background

The nasopharyngeal microbiota represents one of the key factors related to infectious diseases in children [1]. The infectious agents and their resistance patterns are main factors driving disease severity. Nasopharyngeal carriage is high in children, especially for Staphylococcus aureus [2, 3].

Methods

We performed a screening study for nasopharyngeal carriage of Staphylococcus spp. in immunocompetent children aged 7-10 years old, attending a community school in central Bucharest (group 1), and in two groups of immunosuppressed children: children with hemato-oncologic diseases (lymphoma/leukemia) admitted to the Fundeni Clinical Institute, Bucharest (ages 2-10 years, group 2), and institutionalized children with vertically transmitted HIV infection, from the National Institute for Infectious Diseases “Prof. Dr. Matei Balş”, Bucharest (ages 1-10 years, group 3).

Results

We analyzed data from 139 pharyngeal swabs (35.3% in group 1, 56.1% group 2 and 8.6% group 3), and 143 nasal swabs (37.1% group 1, 54.5% group 2 and 8.4% group 3). Pharyngeal cultures were positive for Staphylococcus spp. in 28.6% of children in group 1, 11.5% in group 2 (p=0.00755 vs. group 1) and 16.7% in group 3 (p=0.20045 vs. group 1). Of the positive pharyngeal samples, 92.9% were S. aureus in group 1, 100% in group 2 and 100% in group 3.

Nasal cultures were positive for Staphylococcus spp. in 84.9% of children in group 1, 48.7% group 2 (p=0 vs. group 1) and 50.0% group 3 (p=0.00391 vs. group 1). Of the positive nasal samples, 62.2% were identified as S. aureus in group 1, 94.6% in group 2 (p=0.00027 vs. group 1) and 66.7% in group 3.

Conclusion

Pharyngeal carriage of Staphylococcus strains was low, however when positive, most strains were S. aureus. Nasopharyngeal carriage was significantly higher in immunocompetent children from the community compared to immunodepressed children. When present, S. aureus had a higher prevalence compared to coagulase-negative staphylococci (CoNS), particularly in immunodepressed children.

Declarations

Acknowledgements

1) POSDRU/159/1.5/S/137390.

2) Carol Davila University of Medicine and Pharmacy, Young Researchers Projects Grant Competition, contract number 28.335/04.11.2013.

Authors’ Affiliations

(1)
Carol Davila University of Medicine and Pharmacy
(2)
National Institute for Infectious Diseases "Prof. Dr. Matei Balş"
(3)
Dental Concept Studio

References

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  3. Preoțescu LL, Streinu-Cercel O: Prevalence of nasal carriage of S aureus in children. GERMS. 2013, 3: 49-51. 10.11599/germs.2013.1035.View ArticleGoogle Scholar

Copyright

© Streinu-Cercel et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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