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Complete genome sequence of new unique recombinant HIV type 1 isolated from a child born to an HIV-infected mother

Introduction

In our previously studies we detect a HIV recombinant form with BgagApolAenv by isolate named Mos. This virus differs from CRF02_AB with AgagBpolBenv. For more detailed studying of isolate Mos genome we carried out complete genome sequencing. (Figure 1)

figure1

Figure 1

Materials and methods

HIV-1 RNA was extracted from plasma, reverse transcribed, and amplified using sets of primers designed to span the complete genome of HIV-1. Contig assembled in SeqMan®, DNAStar. Recombination analysis was performed using REGA HIV Subtyping Tool. Phylogenetic analysis was made in MEGA4 software with Kimura-2-parametres.

Results

Analysis of complete genome shows that recombinant Mos is a new HIV-1 URF with gag/pol mosaic structure composed of parental subtypes A and B and rest genome composed of subtype A. Phylogenetical analysis had shown that HIV-1 subtype A ancestor is AY500393 Russia, subtype B - DQ207943 Georgia.

Conclusions

This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from Russia and Georgia.

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Correspondence to S Sasinovich.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sasinovich, S., Eremin, V., Gasich, E. et al. Complete genome sequence of new unique recombinant HIV type 1 isolated from a child born to an HIV-infected mother. BMC Infect Dis 14, P62 (2014). https://doi.org/10.1186/1471-2334-14-S2-P62

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Keywords

  • Public Health
  • Internal Medicine
  • Infectious Disease
  • Phylogenetic Analysis
  • Genome Sequence