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  • Open Access

Complete genome sequence of new unique recombinant HIV type 1 isolated from a child born to an HIV-infected mother

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BMC Infectious Diseases201414 (Suppl 2) :P62

https://doi.org/10.1186/1471-2334-14-S2-P62

  • Published:

Keywords

  • Public Health
  • Internal Medicine
  • Infectious Disease
  • Phylogenetic Analysis
  • Genome Sequence

Introduction

In our previously studies we detect a HIV recombinant form with BgagApolAenv by isolate named Mos. This virus differs from CRF02_AB with AgagBpolBenv. For more detailed studying of isolate Mos genome we carried out complete genome sequencing. (Figure 1)

Figure 1

Materials and methods

HIV-1 RNA was extracted from plasma, reverse transcribed, and amplified using sets of primers designed to span the complete genome of HIV-1. Contig assembled in SeqMan®, DNAStar. Recombination analysis was performed using REGA HIV Subtyping Tool. Phylogenetic analysis was made in MEGA4 software with Kimura-2-parametres.

Results

Analysis of complete genome shows that recombinant Mos is a new HIV-1 URF with gag/pol mosaic structure composed of parental subtypes A and B and rest genome composed of subtype A. Phylogenetical analysis had shown that HIV-1 subtype A ancestor is AY500393 Russia, subtype B - DQ207943 Georgia.

Conclusions

This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from Russia and Georgia.

Authors’ Affiliations

(1)
RRPC for Epidemiology and Microbiology, Minsk, Belorussia

Copyright

© Sasinovich et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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