Open Access

Erratum To: Production of recombinant Entamoeba histolyticapyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess

  • Syazwan Saidin1,
  • Muhammad Hafiznur Yunus1,
  • Nor Dyana Zakaria1,
  • Khairunisak Abdul Razak2, 3,
  • Lim Boon Huat4,
  • Nurulhasanah Othman1 and
  • Rahmah Noordin1Email author
BMC Infectious Diseases201414:533

https://doi.org/10.1186/1471-2334-14-533

Received: 4 September 2014

Accepted: 9 September 2014

Published: 10 November 2014

The original article was published in BMC Infectious Diseases 2014 14:182

Correction

After publication of this article [1], it was brought to our attention that we had failed to make it clear that Figures one (Figure 1 here), two (Figure 2 here) and three (Figure 3 here) were put together by grouping elements from different images. The revised figures, in which this grouping has been made explicit in the arrangement, and figure legends, in which the adjustments are clearly stated, are shown below.
Figure 1

SDS-PAGE analysis shows the purified rPPDK. Lane 1: Precision Plus Protein™ Unstained Standard Marker (Bio-Rad, USA); Lane 2: Non-induced cell from pET28a/PPDK; Lane 3: Induced cell from pET28a/PPDK; Lanes 4–5: purified rPPDK protein from elution buffer (250 mM imidazole). Arrow indicates the recombinant PPDK protein (~98 kDa). Figure one (Figure 1 here) was made by grouping elements from the original Images 1A and 1B as follows: Lanes 1, 2 and 3 in Figure one (Figure 1 here) came from lanes M, 6 and 8 respectively in Image 1A; while Lanes 4 and 5 in Figure one (Figure 1 here) came from lanes 7 and 8 respectively in Image 1B.

Figure 2

SDS-PAGE analysis shows the purified rGal/GalNAc lectin. Lane 1: Precision Plus Protein™ Unstained Standard Marker (Bio-Rad, USA); Lane 2: Non-induced cell from pET28a/Gal-GalNAc lectin; Lane 3: Induced cell from pET28a/Gal-GalNAc lectin; Lanes 4–5: purified rGal/GalNAc lectin protein from elution buffer (250 mM imidazole). Arrow indicates the recombinant Gal/GalNAc lectin protein (~53 kDa). Figure two (Figure 2 here) was made by grouping elements from the original image 1A as follows: Lanes 1, 2, 3, 4, and 5 in Figure two (Figure 2 here) came from lanes M, 1, 2, 3 and 4 respectively in Image 1A.

Figure 3

Representative rPPDK-LFD strips tested with serum samples. Lanes 1–3: Lateral flow test strips tested with serum samples from patients with ALA; Lane 4: strip tested with serum from healthy individual; Lanes 5–6: strips tested with serum samples from other diseases (i.e. malaria and pyogenic liver abscess); C: control line; T: test line. Figure six (Figure 3) was made by grouping elements from original Images 6A and 6B as follows: Dipstick 1 and 2 in Figure six (Figure 3) came from dipsticks 3 and 4 in Image 6A; dipstick 3 in Figure six (Figure 3) came from dipstick 4 in Image 6B; dipsticks 4, 5 and 6 in Figure six (Figure 3) came from dipsticks 5, 6 and 7 respectively in Image 6A.

We regret any inconvenience that this inaccuracy in the figures in the original manuscript might have caused.

Notes

Declarations

Authors’ Affiliations

(1)
Institute for Research in Molecular Medicine, Universiti Sains Malaysia
(2)
School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia
(3)
NanoBiotechnology Research and Innovation (NanoBRI) Institute for Research in Molecular Medicine, Universiti Sains Malaysia
(4)
School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian

References

  1. Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R: Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis. 2014, 14: 182-10.1186/1471-2334-14-182.View ArticlePubMedPubMed CentralGoogle Scholar
  2. Pre-publication history

    1. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/533/prepub

Copyright

© Saidin et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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