This article has Open Peer Review reports available.
Acute and probable chronic Q fever during anti-TNFα and anti B-cell immunotherapy: a case report
© Schoffelen et al.; licensee BioMed Central Ltd. 2014
Received: 19 November 2013
Accepted: 9 June 2014
Published: 15 June 2014
Q fever is caused by the intracellular bacterium Coxiella burnetii. Initial infection can present as acute Q fever, while a minority of infected individuals develops chronic Q fever endocarditis or vascular infection months to years after initial infection. Serology is an important diagnostic tool for both acute and chronic Q fever. However, since immunosuppressive drugs may hamper the humoral immune response, diagnosis of Q fever might be blurred when these drugs are used.
A 71-year-old Caucasian male was diagnosed with symptomatic acute Q fever (based on positive C. burnetii PCR followed by seroconversion) while using anti-tumor necrosis factor-α (anti-TNFα) drugs for rheumatoid arthritis (RA). He was treated for two weeks with moxifloxacin. After 24 months of follow-up, the diagnosis of probable chronic Q fever was established based on increasing anti-C. burnetii phase I IgG antibody titres in a immunocompromised patient combined with clinical suspicion of endocarditis. At the time of chronic Q fever diagnosis, he had been treated with anti B-cell therapy for 16 months. Antibiotic therapy consisting of 1.5 years doxycycline and hydroxychloroquine was started and successfully completed and no signs of relapse were seen after more than one year of follow-up.
The use of anti-TNFα agents for RA in the acute phase of Q fever did not hamper the C. burnetii-specific serological response as measured by immunofluorescence assay. However, in the presented case, an intact humoral response did not prevent progression to probable chronic C. burnetii infection, most likely because essential cellular immune responses were suppressed during the acute phase of the infection. Despite the start of anti-B-cell therapy with rituximab after the acute Q fever episode, an increase in anti-C. burnetii phase I IgG antibodies was observed, supporting the notion that C. burnetii specific CD20-negative memory B-cells are responsible for this rise in antibody titres.
Q fever is caused by the intracellular growing bacterium Coxiella burnetii. Acute Q fever is a (self-limiting) febrile illness, but can present as pneumonia or hepatitis. Chronic Q fever presents most often as an endovascular infection, i.e. endocarditis or mycotic aneurysm or infected vascular graft, which has a high mortality if left untreated [2, 3]. Risk factors are underlying valvular defects, or pre-existing vascular aneurysm or prosthesis.
Immunosuppression is another stated risk factor for chronic Q fever, as some immunosuppressive drugs decrease protective cellular responses against intracellular growing bacteria. This risk factor has thus far been poorly documented, but recently we confirmed that patients with rheumatoid arthritis (RA) using immunosuppressive drugs are indeed at increased risk of developing chronic Q fever .
Clinical signs of C. burnetii infection are often nonspecific, and the diagnosis of acute or chronic Q fever is heavily based on measurement of antibody titres [5, 6], complemented by the direct detection of the micro-organism by polymerase chain reaction (PCR) [7, 8]. Serologic criteria for Q fever consist of measurement of antibodies against the two antigenic forms of C. burnetii, phase I and II organisms, with high anti-C. burnetii phase I IgG titres - in the absence of acute Q fever – pointing to a chronic infection. The appropriate cut-off titre that differentiates it from a past cleared infection is debated; currently proposed cut-offs are 1:1,024 or 1:1,600 [6, 9].
The diagnosis of Q fever in hosts on immunosuppressive drugs may be complicated, because these drugs can inhibit antibody responses and therefore hamper correct diagnosis based on serologic results. Also the immune-mediated disease itself, for which these drugs are prescribed, may contribute to inadequate immune responses to infection [10, 11].
Here we present a case history of a patient with RA who had an episode of acute Q fever while being treated with anti-tumor necrosis factor-α (anti-TNFα) medication, and who developed probable chronic Q fever over the subsequent two years while using the anti-B-cell monoclonal antibody rituximab. The case highlights the importance of cellular and humoral immune response modifying agents in the natural course of C. burnetii infections and the possible pitfalls of the use of serological methods to detect the stage of disease.
Acute Q fever
In May 2009, during the Dutch Q fever epidemic, a 71-years-old rheumatoid factor and anti-CCP positive RA patient living in the Q fever high incidence area, presented with 8 days of fever and a non-productive cough. He was receiving anti-rheumatic treatment including etanercept (an anti-tumor necrosis factor-α [anti-TNFα] agent) and prednisone. He had a history of atrial fibrillation, but no underlying valvulopathy. Physical examination and a chest X-ray were compatible with a pulmonary infiltrate. No murmurs were heard upon cardiac auscultation. Laboratory investigations revealed increased C-reactive protein (CRP, 285 mg/L), a normal leukocyte count (5.2×109/L) and normal values for renal function and liver enzymes. PCR (real-time PCR targeting the IS1111a insertion element ) for C. burnetii on plasma turned out to be positive. However, serology (immunofluorescence assay [IFA, Focus Diagnostics, Cypress, USA]) was negative for IgM as well as for IgG against phase I and II Coxiella burnetii. The diagnosis of acute Q fever was made and treatment with moxifloxacin 400 mg daily for 14 days was started. Two weeks later, seroconversion was observed with anti-phase I and II IgM titres of 1:4096 and 1:16384 respectively.
Diagnosis and treatment of probable chronic Q fever
After quick recovery, the patient was followed-up to monitor for possible progression to chronic Q fever. During this period, the anti-rheumatic treatment had been switched by the rheumatologist from etanercept to adalimumab (another anti-TNFα agent), and subsequently – 8 months after the acute Q fever episode – to rituximab. The latter, an anti-CD20 anti B-cell monoclonal antibody, had resulted in adequate suppression of the rheumatic activity.
We report on an immunocompromised patient, followed-up from the start of a symptomatic acute Q fever episode to the development and treatment of probable chronic Q fever. Our case illustrates three interesting aspects of diagnosis and treatment of Q fever in an immunocompromised host. First, we noticed that, despite the presence of RA and use of anti-TNFα agents, the humoral response to the initial C. burnetii infection was not impaired. Secondly, in spite of adequate treatment, the acute Q fever progressed to probable chronic Q fever endocarditis, which suggests incomplete clearance of the infection during the acute stage. Finally, the increase in phase I IgG titres, which serves as a marker for chronic Q fever, occurred under treatment with anti B-cell immunotherapy which was started after the acute Q fever episode.
In the presented case, a definite diagnosis of chronic Q fever could not be made. C. burnetii DNA was not detected in blood on several occasions and echocardiography did not show major signs of endocarditis. Nevertheless, chronic Q fever was clinically highly suspected. The patient was followed-up after acute Q fever, and we observed that the antibody titres were falling after they had peaked following the acute Q fever episode, only to rise again after 18 months. This increase in antibody titres was accompanied by aspecific complaints of fatigue and a newly diagnosed cardiac murmur. According to the Dutch guidelines, this was a diagnosis of probable chronic Q fever , and the patient received antibiotic treatment as considered appropriate.
Interestingly, we observed a normal antibody response in the acute phase of the Q fever infection under anti-TNFα therapy. This is in line with previous studies which have shown that anti-TNFα therapy does not prevent serologic responses to influenza vaccination [14, 15], although titres may be somewhat lower. Clearly, in our case, this normal antibody response did not prevent the development of persistent C. burnetii infection, as complete clearance might depend more on cellular immune responses.
Indeed, to constrain intracellular C. burnetii infections, a cellular immune response is crucial and TNFα is a key cytokine in this response. In-vitro studies showed that TNFα mediates interferon-gamma induced intracellular killing of C. burnetii in monocytes through apoptosis . TNF knock-out mice infected with C. burnetii develop early bactaeremia and severe heart lesions . Infection risk due to decreased cellular immunity in anti-TNFα treated patients, has been shown for other intracellular infections, most notably Mycobacterium tuberculosis, but also Listeria monocytogenes and Salmonella enterica, and for herpesviridae [18–21].
Rituximab, an anti-CD20 monoclonal antibody, is used for the treatment of RA patients failing on TNFα blockers. Rituximab depletes circulating CD20-positive B-cells for a period of six to nine months . As a consequence, patients on rituximab therapy have an impaired antibody response to neo-antigens. Existing plasma cells and memory B-cells, which do not express CD20, are not affected by rituximab . During the development from acute to chronic Q fever, there is an ongoing infection with presumably increasing concentrations of C. burnetii antigens. Because plasma cells do not express B-cell receptors at their surface, which makes them incapable to respond to alterations in antigen concentrations, we assume that the rise of anti-C. burnetii IgG titres in our patient originated from stimulation of memory IgG B-cells by increased concentrations of recall antigens. This intact response to recall antigens after rituximab has been observed for patients receiving vaccinations , but has never been documented after natural infection.
Our results indicate that anti-C. burnetii phase I IgG antibody titres can be used as a marker for progression to chronic Q fever and the subsequent response to therapy in patients in whom B-cell depleting therapy is started after initial exposure. However, it is likely that B-cell depleting medication during first contact with neo-antigens of C. burnetii will seriously hamper the development of an antibody response and the diagnosis of Q fever based on serological titres.
The use of anti-TNFα agents for RA in the acute phase of Q fever does not seem to impede the C. burnetii-specific serological response. However, in the presented case, an intact humoral response did not prevent progression to probable chronic C. burnetii infection, most likely because essential cellular immune responses were suppressed in the acute phase of the infection. Even though anti-B-cell therapy with rituximab was started after the acute Q fever episode, an increase in anti-C. burnetii phase I antibodies was observed, supporting the notion that C. burnetii specific CD20-negative memory B-cells are responsible for this rise in antibody titres.
Written informed consent was obtained from the patient for publication of this Case report. A copy of the written consent is available for review by the Editor of this journal.
Bea Groezen, Dorien van Gülick and Mary Smolders are gratefully acknowledged for their technical support in performing the serological assays.
This work was supported by The Netherlands Organization for Health Research and Development [grant number 205520002 to TSc].
- Parker NR, Barralet JH, Bell AM: Q fever. Lancet. 2006, 367 (9511): 679-688. 10.1016/S0140-6736(06)68266-4.View ArticlePubMedGoogle Scholar
- Raoult D, Marrie T, Mege J: Natural history and pathophysiology of Q fever. Lancet Infect Dis. 2005, 5 (4): 219-226. 10.1016/S1473-3099(05)70052-9.View ArticlePubMedGoogle Scholar
- Botelho-Nevers E, Fournier PE, Richet H, Fenollar F, Lepidi H, Foucault C, Branchereau A, Piquet P, Maurin M, Raoult D: Coxiella burnetii infection of aortic aneurysms or vascular grafts: report of 30 new cases and evaluation of outcome. Eur J Clin Microbiol Infect Dis. 2007, 26 (9): 635-640. 10.1007/s10096-007-0357-6.View ArticlePubMedGoogle Scholar
- Schoffelen T, Kampschreur LM, van Roeden SE, Wever PC, den Broeder AA, Nabuurs-Franssen MH, Sprong T, Joosten LA, van Riel PL, Oosterheert JJ, van Deuren M, Creemers MC: Coxiella burnetiiinfection (Q fever) in rheumatoid arthritis patients with and without anti-TNFα therapy.Ann Rheum Dis. 2014, 73 (7): 1436-1438. 10.1136/annrheumdis-2014-205455.View ArticlePubMedGoogle Scholar
- Landais C, Fenollar F, Thuny F, Raoult D: From acute Q fever to endocarditis: serological follow-up strategy. Clin Infect Dis. 2007, 44 (10): 1337-1340. 10.1086/515401.View ArticlePubMedGoogle Scholar
- van der Hoek W, Versteeg B, Meekelenkamp JC, Renders NH, Leenders AC, Weers-Pothoff I, Hermans MH, Zaaijer HL, Wever PC, Schneeberger PM: Follow-up of 686 patients with acute Q fever and detection of chronic infection. Clin Infect Dis. 2011, 52 (12): 1431-1436. 10.1093/cid/cir234.View ArticlePubMedGoogle Scholar
- Fournier PE, Raoult D: Comparison of PCR and serology assays for early diagnosis of acute Q fever. J Clin Microbiol. 2003, 41 (11): 5094-5098. 10.1128/JCM.41.11.5094-5098.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Fenollar F, Fournier PE, Raoult D: Molecular detection of Coxiella burnetii in the sera of patients with Q fever endocarditis or vascular infection. J Clin Microbiol. 2004, 42 (11): 4919-4924. 10.1128/JCM.42.11.4919-4924.2004.View ArticlePubMedPubMed CentralGoogle Scholar
- Frankel D, Richet H, Renvoisé A, Raoult D: Q fever in France, 1985–2009. Emerg Infect Dis. 2011, 17 (3): 350-356. 10.3201/eid1703.100882.View ArticlePubMedPubMed CentralGoogle Scholar
- Cobb S, Anderson F, Bauer W: Length of life and cause of death in rheumatoid arthritis. N Engl J Med. 1953, 249 (14): 553-556. 10.1056/NEJM195310012491402.View ArticlePubMedGoogle Scholar
- Uddin J, Kraus AS, Kelly HG: Survivorship and death in rheumatoid arthritis. Arthritis Rheum. 1970, 13 (2): 125-130. 10.1002/art.1780130204.View ArticlePubMedGoogle Scholar
- Tilburg JJ, Melchers WJ, Pettersson AM, Rossen JW, Hermans MH, van Hannen EJ, Nabuurs-Franssen MH, de Vries MC, Horrevorts AM, Klaassen CH: Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum. J Clin Microbiol. 2010, 48 (11): 3923-3927. 10.1128/JCM.01006-10.View ArticlePubMedPubMed CentralGoogle Scholar
- Wegdam-Blans MC, Kampschreur LM, Delsing CE, Bleeker-Rovers CP, Sprong T, van Kasteren ME, Notermans DW, Renders NH, Bijlmer HA, Lestrade PJ, Koopmans MP, Nabuurs-Franssen MH, Oosterheert JJ: Chronic Q fever: review of the literature and a proposal of new diagnostic criteria. J Infect. 2012, 64 (3): 247-259. 10.1016/j.jinf.2011.12.014.View ArticlePubMedGoogle Scholar
- Gelinck LB, van der Bijl AE, Beyer WE, Visser LG, Huizinga TW, van Hogezand RA, Rimmelzwaan GF, Kroon FP: The effect of anti-tumour necrosis factor alpha treatment on the antibody response to influenza vaccination. Ann Rheum Dis. 2008, 67 (5): 713-716.View ArticlePubMedGoogle Scholar
- Kapetanovic MC, Saxne T, Nilsson JA, Geborek P: Influenza vaccination as model for testing immune modulation induced by anti-TNF and methotrexate therapy in rheumatoid arthritis patients. Rheumatology (Oxford). 2007, 46 (4): 608-611.View ArticleGoogle Scholar
- Dellacasagrande J, Capo C, Raoult D, Mege JL: IFN-gamma-mediated control of Coxiella burnetii survival in monocytes: the role of cell apoptosis and TNF. J Immunol. 1999, 162 (4): 2259-2265.PubMedGoogle Scholar
- Andoh M, Zhang G, Russell-Lodrigue KE, Shive HR, Weeks BR, Samuel JE: T cells are essential for bacterial clearance, and gamma interferon, tumor necrosis factor alpha, and B cells are crucial for disease development in Coxiella burnetii infection in mice. Infect Immun. 2007, 75 (7): 3245-3255. 10.1128/IAI.01767-06.View ArticlePubMedPubMed CentralGoogle Scholar
- Gómez-Reino JJ, Carmona L, Angel Descalzo M, Group B: Risk of tuberculosis in patients treated with tumor necrosis factor antagonists due to incomplete prevention of reactivation of latent infection. Arthritis Rheum. 2007, 57 (5): 756-761. 10.1002/art.22768.View ArticlePubMedGoogle Scholar
- Peña-Sagredo JL, Hernández MV, Fernandez-Llanio N, Giménez-Ubeda E, Muñoz-Fernandez S, Ortiz A, Gonzalez-Gay MA, Fariñas MC, Group B: Listeria monocytogenes infection in patients with rheumatic diseases on TNF-alpha antagonist therapy: the Spanish study group experience. Clin Exp Rheumatol. 2008, 26 (5): 854-859.PubMedGoogle Scholar
- Netea MG, Radstake T, Joosten LA, van der Meer JW, Barrera P, Kullberg BJ: Salmonella septicemia in rheumatoid arthritis patients receiving anti-tumor necrosis factor therapy: association with decreased interferon-gamma production and Toll-like receptor 4 expression. Arthritis Rheum. 2003, 48 (7): 1853-1857. 10.1002/art.11151.View ArticlePubMedGoogle Scholar
- Che H, Lukas C, Morel J, Combe B: Risk of herpes/herpes zoster during anti-tumor necrosis factor therapy in patients with rheumatoid arthritis. Joint Bone Spine. 2014, 81 (3): 215-221. 10.1016/j.jbspin.2013.07.009.View ArticlePubMedGoogle Scholar
- Edwards JC, Szczepanski L, Szechinski J, Filipowicz-Sosnowska A, Emery P, Close DR, Stevens RM, Shaw T: Efficacy of B-cell-targeted therapy with rituximab in patients with rheumatoid arthritis. N Engl J Med. 2004, 350 (25): 2572-2581. 10.1056/NEJMoa032534.View ArticlePubMedGoogle Scholar
- Silverman GJ, Weisman S: Rituximab therapy and autoimmune disorders: prospects for anti-B cell therapy. Arthritis Rheum. 2003, 48 (6): 1484-1492. 10.1002/art.10947.View ArticlePubMedGoogle Scholar
- Bingham CO, Looney RJ, Deodhar A, Halsey N, Greenwald M, Codding C, Trzaskoma B, Martin F, Agarwal S, Kelman A: Immunization responses in rheumatoid arthritis patients treated with rituximab: results from a controlled clinical trial. Arthritis Rheum. 2010, 62 (1): 64-74. 10.1002/art.25034.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/330/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.