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A rare case of cytomegalovirus, scedosporium apiospermum and mycobacterium tuberculosis in a renal transplant recipient
© Rathi et al.; licensee BioMed Central Ltd. 2014
Received: 18 November 2013
Accepted: 7 March 2014
Published: 14 May 2014
Renal transplant recipients are at high risk of developing multiple infections, often concomitantly because of their immunocompromised status. Post renal transplant infections are often elusive and require extensive evaluation for proper diagnosis and treatment. A high index of suspicion is required and an attempt should be made to confirm the microbiological diagnosis from each site involved to rule out multiple infections.
We report a 50-year-old female, a renal allograft recipient who presented with left hemiplegia, esophageal ulcers and fever 3 months after her transplant. Esophageal biopsy revealed Cytomegalovirus (CMV) inclusions and the whole blood quantitative CMV polymerase chain reaction (PCR) was positive. Neuroimaging showed a brain abscess, stereotactic biopsy from which revealed Scedosporium apiospermum on fungal culture. Her tacrolimus and mycophenolate were stopped and she was managed with intravenous ganciclovir and voriconazole. With these measures, she showed marked improvement in her general and neurological condition. Two months later, she developed recurrence of fever with dry cough. Radiological investigation revealed a cavitating lung lesion, a needle aspiration from which demonstrated acid-fast bacilli. She was started on antituberculous treatment. With these measures, she recovered completely and maintained good graft function despite being on only prednisolone 10 mg once a day.
Although CMV disease is not uncommon in the first three months post transplant, Scedosporium is a rare cause of brain abscess. On the other hand, tuberculosis is common in transplant recipients, especially in developing countries, like India. However, this is the first case report of occurrence of these three infections in the same patient, demonstrating the importance of a good microbiological work-up from each site involved in immunosuppressed subjects.
Renal transplantation is the best option for patients with end-stage kidney disease, more so for those in developing countries where facilities for maintenance dialysis are inadequate . Although survival rates after renal transplantation have improved considerably, infections remain a major cause of morbidity and mortality in countries with poor socioeconomic conditions. Studies from such countries have reported infection rates in the range of 50-80% and a mortality rate of 20-60%, particularly with severe opportunistic and fungal infections [2, 3]. Various bacterial, viral, fungal and mycobacterial infections may occur simultaneously or sequentially and tissue sampling is often the only mode of conclusive diagnosis. Cytomegalovirus (CMV) and mycobacterial infections are common and well described post renal transplant infections, particularly in the period of intense immunosuppression; however, Scedosporium is a rare fungal pathogen affecting renal transplant recipients. We report a rare case of CMV, Scedosporium apiospermum, and Mycobacterium tuberculosis infection in a renal transplant recipient, all of which were managed successfully.
The risk of infections in transplant recipients is determined by the intensity of epidemiologic exposure to potential pathogens and the net state of immunosuppression. The reasons for higher rates of infection in developing countries include factors like unhygienic conditions, hot and humid environment, overcrowding, high prevalence of endemic infections like tuberculosis, malnutrition, late presentation, unavailability of costly diagnostic investigations like polymerase chain reaction and high cost of life saving antimicrobials [2, 3].
CMV is the most frequent pathogen encountered in renal transplant recipients in countries with a temperate climate. CMV infection usually occurs during the second to fourth month period after transplantation; however, delayed onset can be seen in patients on chemoprophylaxis [4, 5]. The infection can either be asymptomatic or cause illness of varying severity, depending upon the serological status of the recipient and the donor. In our case, the renal transplant was done at another centre and therefore the pretransplant CMV sero-status of the donor and recipient was not known and she also did not receive CMV chemoprophylaxis. CMV is a potentially preventable disease with the use of prophylactic therapy, especially in high-risk cases, those who receive an induction therapy and who require potent anti-rejection therapy  and we feel that the present case would have benefitted by the use of chemoprophylaxis.
Brain abscess is an uncommon infectious complication in renal transplant recipients. Selby et al.  described disparate groups of patients with brain abscesses with regard to the timing, susceptibility and predisposition. He found that while fungi (ie, Aspergillus, Candida, and Mucorales sp) contributed to most of the early onset brain abscesses (median 24 days); abscesses that developed late after transplantation (mean 264 days) were caused by non fungal organisms (i.e., Nocardia and Toxoplasma sp). In another report, Aspergillus species were the most commonly isolated organism .
Scedosporium apiospermum is the anamorph of the ascomycete Pseudallescheria boydii which is a genus of the Ascomycete order Microascales. Scedosporium infections account for about 3% of all fungal infections in post transplant patients with 70% mortality . Scedosporium usually causes cutaneous infection; however, sometimes it can cause a disseminated infection [10–12], although isolated brain infection is rare. Being commonly found in heavily polluted environments, agricultural and garden soil, sewer, ditch mud and polluted pond bottoms, drowning is a common cause for brain infection with this fungus . It is commonly misdiagnosed as Aspergillus due to its septate hyphal morphology. A search of Pubmed and EMBASE has revealed about eight cases of post renal transplant brain scedosporiosis [11, 12, 14–19].
Our case did not have any known exposure to above mentioned environmental factors nor had past history of any other fungal infection. She was managed successfully with voriconazole therapy. Voriconazole produces a quick and sustained response with cure rates of up to 57% in immunocompromised subjects with those having brain infection being the worst responders . The infection may persist in the body for long periods of time and has been reported to recur after a second transplant [17, 21] and therefore it might be appropriate to give prolonged secondary chemoprophylaxis.
The incidence of post transplant tuberculosis varies from 0.48% in the west  to 11.8% in India . Therapeutic response is often satisfactory and drug interactions mandate dose adjustment of other immunosuppression drugs as well as azoles. British thoracic society and European Best Practice Guidelines recommend rifampicin-based four drug therapy for 6 months with blood level monitoring for calcineurin inhibitors [24, 25]. Similarly, if the patient is on azoles, the drug levels should be monitored. Although pretransplant evaluation and treatment for latent tuberculosis is recommended, in highly endemic countries like ours, both of these strategies are faced with problems. Due to high endemicity and an increased chance of exposure, evaluation for latent tuberculosis has been shown to be nonspecific and insensitive . Similarly, the treatment of latent tuberculosis by using isoniazid monotheraphy is prone for the development of resistant tuberculosis  and there is no consensus on whether latent tuberculosis should be treated in countries otherwise endemic for this infection .
Opportunistic infections in post transplant subjects are very common and require high index of suspicion and proper investigative approach for early diagnosis and treatment. Co-infections can be a diagnostic problem as one infection can easily undermine the existence of the other. In the presence of CMV infection, a co-existent fungal infection needs careful exclusion. Pharmacodynamic monitoring with maintenance of therapeutic levels of immunosuppressive drugs within the prescribed range and tailoring of immunosuppression as per the immunological risk of the patient may provide the long sought balance of adequate immunosuppression with minimal opportunistic infections and malignancies.
Our case appears to be the first in the literature with the triad of CMV, Scedosporium apiospermum and Mycobacterium tuberculosis infection, all of which were managed successfully. In the present case, since a CT of the chest was not done during the first admission, the probability of pulmonary tuberculosis being there at that time itself cannot be ruled out, though it manifested two months later. This case highlights the importance of getting a microbiological diagnosis from each site involved. One should not assume that if one organism has been isolated, the other manifestations are also because of the same organism. This case also exemplifies the need for tailored immunosuppression for renal transplant recipients to reduce their susceptibility to infections.
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor of this journal.
- Chugh KS, Jha V: Differences in the care of ESRD patients worldwide: required resources and future outlook. Kidney Int. 1995, 48 (suppl 50): S7-13.Google Scholar
- Gueco I, Saniel M, Mendoza M, Alano F, Ona E: Tropical infections after renal transplantation. Transplant Proc. 1989, 21 (1 pt 2): 2105-2107.PubMedGoogle Scholar
- Chugh KS, Sakhuja V, Jain S, Talwar P, Minz M, Joshi K, Indudhara R: High mortality in systemic fungal infections following renal transplantation in third-world countries. Nephrol Dial Transplant. 1993, 8: 168-172.PubMedGoogle Scholar
- Rao M: Cytomegalovirus infection after renal transplantation - the Indian experience. Indian J Nephro. 2002, 12: 16-24.Google Scholar
- Pescovitz MD: Benefits of cytomegalovirus prophylaxis in solid organ transplantation. Transplantation. 2006, 82 (2 Suppl): S4-8.View ArticlePubMedGoogle Scholar
- Rubin RH: The pathogenesis and clinical management of cytomegalovirus infection in the organ transplant recipient: the end of the ‘silo hypothesis’. Curr Opin Infect Dis. 2007, 20: 399-407. 10.1097/QCO.0b013e328285a358.View ArticlePubMedGoogle Scholar
- Selby R, Ramirez CB, Singh R, Kleopoulos I, Kusne S, Starzl TE, Fung J: Brain abscess in solid organ transplant recipients receiving cyclosporine-based immunosuppression. Arch Surg. 1997, 132: 304-310. 10.1001/archsurg.1997.01430270090019.View ArticlePubMedPubMed CentralGoogle Scholar
- Baddley JW, Salzman D, Pappas PG: Fungal brain abscess in transplant recipients: epidemiologic, microbiologic, and clinical features. Clin Transplant. 2002, 16: 419-424. 10.1034/j.1399-0012.2002.02033.x.View ArticlePubMedGoogle Scholar
- Castiglioni B, Sutton DA, Rinaldi MG, Fung J, Kusne S: Pseudallscheria boydii (Anamorph Scedosporium apiospermum) infection in organ transplant recipients in a tertiary medical center and review of the literature. Medicine. 2002, 81: 333-348. 10.1097/00005792-200209000-00001.View ArticlePubMedGoogle Scholar
- Rogasi PG, Zanazzi M, Nocentini J, Fantoni E, Trotta M, Faggi E, Fontanelli A, Bertoni E, Salvadori M, Leoncini F: Disseminated scedosporium apiospermum infection in renal transplant recipient: long-term successful treatment with voriconazole: a case report. Transplant Proc. 2007, 39: 2033-2035. 10.1016/j.transproceed.2007.05.044.View ArticlePubMedGoogle Scholar
- Campagnaro EL, Woodside KJ, Early MG, Gugliuzza KK, Colomé-Grimmer MI, Lopez FA, Daller JA: Disseminated pseudallescheria boydii (scedosporium apiospermum) infection in a renal transplant patient. Transpl Infect Dis. 2002, 4: 207-211. 10.1034/j.1399-3062.2002.t01-1-01012.x.View ArticlePubMedGoogle Scholar
- Walker DH, Adamec T, Krigman M: Disseminated petriellidosis (allescheriasis). Arch Pathol Lab Med. 1978, 102: 158-160.PubMedGoogle Scholar
- Buzina W, Feierl G, Haas D, Reinthaler FF, Holl A, Kleinert R, Reichenpfader B, Roll P, Marth E: Lethal brain abscess due to the fungus scedosporium apiospermum (teleomorph pseudallescheria boydii) after a near-drowning incident: case report and review of the literature. Med Mycol. 2006, 44: 473-477. 10.1080/13693780600654588.View ArticlePubMedGoogle Scholar
- Caya JG, Farmer SG, Williams GA, Franson TR, Komorowski RA, Kies JC: Bilateral pseudallescheria boydii endophthalmitis in an immunocompromised patient. Wis Med J. 1988, 87: 11-14.PubMedGoogle Scholar
- Nesky MA, McDougal EC, Peacock JE: Pseudallescheria boydii brain abscess successfully treated with voriconazole and surgical drainage: case report and literature review of central nervous system pseudallescheriasis. Clin Infect Dis. 2000, 31: 673-677. 10.1086/314042.View ArticlePubMedGoogle Scholar
- Lavarde V, Traore F, Farge D: Brain abscess due to pseudallescheria boydii in a renal transplant patient. Bull Soc Fr Mycol Medical. 1989, 18: 149-152.Google Scholar
- Montejo M, Muniz ML, Zarraga S, Aguirrebengoa K, Amenabar JJ, López-Soria L, Gonzalez R: Infection due to scedosporium apiospermum in renal transplant recipients: a report of two cases and literature review of central nervous system and cutaneous infections by pseudallescheria boydii ⁄ Sc. Mycoses. 2002, 45: 418-427. 10.1046/j.1439-0507.2002.00790.x.View ArticlePubMedGoogle Scholar
- Satirapoj B, Ruangkanchanaster P, Treewatchareekom S, Supasyndh O, Luesutthiviboon L, Supaporn T: Pseudallescheria boydii brain abscess in a renal transplant recipient: first case report in southeast Asia. Transplant Proc. 2008, 40: 2425-2427. 10.1016/j.transproceed.2008.07.030.View ArticlePubMedGoogle Scholar
- Larbcharoensub N, Chongtrakool P, Wirojtananugoon C, Watcharananan SP, Sumethkul V, Boongird A, Jirasiritham S: Treatment of a brain abscess caused by scedosporium apiospermum and phaeoacremonium parasiticum in a renal transplant recipient. Southeast Asian J Trop Med Public Health. 2013, 44: 484-489.PubMedGoogle Scholar
- Troke P, Aguirrebengoa K, Arteaga C, Ellis D, Heath CH, Lutsar I, Rovira M, Nguyen Q, Slavin M, Chen SC, Global Scedosporium Study Group: Treatment of scedosporiosis with voriconazole: clinical experience with 107 patients. Antimicrob Agents Chemother. 2008, 52: 1743-1750. 10.1128/AAC.01388-07.View ArticlePubMedPubMed CentralGoogle Scholar
- Ahmed J, Ditmars DM, Sheppard T, del Busto R, Venkat KK, Parasuraman R: Recurrence of scedosporium apiospermum infection following renal re-transplantation. Am J Transpl. 2004, 4: 1720-1724. 10.1111/j.1600-6143.2004.00576.x.View ArticleGoogle Scholar
- Cisneros JT, Doblas A, Aguado JM, Juan RS, Blanes M, Montejo M: Tuberculosis after solid organ transplant: Incidence, risk factors and clinical characteristics in the RESITRA. Clin Infect Dis. 2009, 48: 1657-1665. 10.1086/599035.View ArticlePubMedGoogle Scholar
- Sakhuja V, Jha V, Varma PP, Joshi K, Chugh KS: The high incidence of tuberculosis among renal transplant recipients in India. Transplantation. 1996, 61: 211-215. 10.1097/00007890-199601270-00008.View ArticlePubMedGoogle Scholar
- Milburn H, Ashman N, Davies P, Doffman S, Drobniewski F, Khoo S, Ormerod P, Ostermann M, Snelson C, British Thoracic Society Standards of Care Committee and Joint Tuberculosis Committee: Guidelines for the prevention and management of mycobacterium tuberculosis infection in adult patients with chronic kidney disease. Thorax. 2010, 65: 557-570.View ArticlePubMedGoogle Scholar
- EBPG Expert Group on Renal Transplantation: European best practice guidelines for renal transplantation. Nephrol Dial Transplant. 2002, 17 (Suppl 4): 39-42.Google Scholar
- Agarwal SK, Gupta S, Bhowmik D, Mahajan S: Tuberculin skin test for the diagnosis of latent tuberculosis during renal replacement therapy in an endemic area: a single center study. Indian J Nephrol. 2010, 20: 132-136. 10.4103/0971-4065.70842.View ArticlePubMedPubMed CentralGoogle Scholar
- Agarwal SK, Gupta S, Dash SC, Bhowmik D, Tiwari SC: Prospective randomised trial of isoniazid prophylaxis in renal transplant recipient. Int Urol Nephrol. 2004, 36: 425-431. 10.1007/s11255-004-6251-6.View ArticlePubMedGoogle Scholar
- API Consensus Expert Committee: API TB consensus guidelines 2006: management of pulmonary tuberculosis, extra-pulmonary tuberculosis and tuberculosis in special situations. J Assoc Physicians India. 2006, 54: 219-234.Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/259/prepub
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