- Research article
- Open Access
- Open Peer Review
Outbreak of Clostridium difficilePCR ribotype 027 - the recent experience of a regional hospital
© Oleastro et al.; licensee BioMed Central Ltd. 2014
- Received: 22 October 2013
- Accepted: 8 April 2014
- Published: 17 April 2014
Clostridium difficile infection (CDI) is the leading cause of healthcare-associated diarrhea, and several outbreaks with increased severity and mortality have been reported. In this study we report a C. difficile PCR ribotype 027 outbreak in Portugal, aiming to contribute to a better knowledge of the epidemiology of this agent in Europe.
Outbreak report with retrospective study of medical records and active surveillance data of all inpatients with the diagnosis of CDI, from 1st January to 31th December 2012, in a Portuguese hospital. C. difficile isolates were characterized regarding ribotype, toxin genes and moxifloxin resistance. Outbreak control measures were taken, concerning communication, education, reinforcement of infection control measures, optimization of diagnosis and treatment of CDI, and antibiotic stewardship.
Fifty-three inpatients met the case definition of C. difficile-associated infection: 55% males, median age was 78.0 years (interquartile range: 71.0-86.0), 75% had co-morbidities, only 15% had a nonfatal condition, 68% had at least one criteria of severe disease at diagnosis, 89% received prior antibiotherapy, 79% of episodes were nosocomial. CDI rate peak was 13.89/10,000 bed days. Crude mortality rate at 6 months was 64.2% while CDI attributable cause was 11.3%. Worse outcome was related to older age (P = 0.022), severity criteria at diagnosis (leukocytosis (P = 0.008) and renal failure), and presence of fatal underlying condition (P = 0.025). PCR ribotype 027 was identified in 16 of 22 studied samples.
This is the first report of a 027-CDI outbreak in Portugal. We emphasize the relevance of the measures taken to control the outbreak and highlight the importance of implementing a close and active surveillance of CDI.
- Clostridium difficile
- PCR ribotype 027
Clostridium difficile is an environmental organism that may be carried asymptomatically in the human intestine, or cause disease in susceptible persons. Those include individuals with disturbed intestinal flora, especially due to recent use of broad-spectrum antibiotics, and/or immunocompromised patients. Also, the risk of C. difficile infection (CDI) is higher among elderly patients who had recent contact with healthcare settings [1–4]. Symptoms of CDI range from mild diarrhea to pseudo-membranous colitis, toxic mega colon or even death. CDI is presently the leading cause of healthcare-associated diarrhea in Europe and in North America, representing an enormous clinical and economic burden . It is also an emerging pathogen in the community, and is spreading as well to animals used in food industry .
Since 2003, large outbreaks of more severe CDI-associated disease, with higher relapse rates and increased mortality have been reported in North America and Europe, which have been attributed to strains of C. difficile belonging to ribotype 027 . These strains were shown to be resistant to fluoroquinolones , and displayed genetic mutations in a toxin regulator gene (tcdC) causing higher expression of toxins A and B, and produced a binary toxin [8, 9]. Nevertheless, the contribution of these factors to the higher virulence of C. difficile is yet to be clarified.
Recently, a large hospital-based survey conducted between 2008 and 2009, involving 106 laboratories in 34 European countries, showed that ribotype 027 was not included among the most prevalent European types, with its overall prevalence showing a slight decrease since the last study (5%) . In that survey, the 027 ribotype was not detected among the isolates from Portuguese patients, but only 12 isolates were studied.
This study reports the first outbreak of C. difficile due to ribotype 027 strain in a Portuguese hospital, aiming to contribute to a better knowledge of the epidemiology of this agent in Europe, and discusses the bundle of outbreak control measures taken.
The present study was considered a retrospective analysis of medical records in the context of an outbreak investigation. Therefore, its execution was communicated to the Ethics Committee of the Centro Hospitalar do Algarve (CHA), but no ethical approval was required.
This study was conducted in the Centro Hospitalar do Algarve (CHA), which is a regional secondary hospital in the South of Portugal, with a capacity of 330 beds, including 40 beds (part of the Internal Medicine Department) located separately, 17 km away from the main building. It serves a population of about 170,000 inhabitants and has an Intensive Care unit (12 beds) and Oncology and Haematology Outpatient units (excluding acute leukemia and bone marrow transplant patients). During the year of 2012, the hospital recorded 15 716 inpatient admissions, with a mean length of stay of 7.17 days.
The CHA comprises an Infection Control Program and Infection Control Team (ICT) and written guidelines for the prevention and treatment of CDI since 2008 that are reviewed every 3 years, as well as for the management of outbreaks. The subject “CDI” is discussed every year in the educational activities for the healthcare workers (HCW) and active surveillance of CDI is conducted since 2009, with a mean incidence rate below 2/10,000 patient bed days until the end of the 1st trimester of 2012.
Data collection and population
We conducted a retrospective study of the medical records and active surveillance data collected by the ICT of all inpatients with the diagnosis of CDI in CHA, during the period from 1st January to 31th December 2012. Demographic, epidemiological and clinical data, co-morbid conditions, antibiotics and proton pump inhibitors (PPI) consumption within the three months prior to diagnosis of CDI, as well as treatment, rate of relapse and 30 day crude and attributable mortality, were registered and analysed in a Microsoft EXCEL™ database.
For case definition, severe disease criteria, and treatment guidelines, the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) treatment guidance for C. difficile infection was used .
The Mccabe score was used to evaluate the vital prognosis of the underlying clinical situation, and comprises three categories: nonfatal disease (five year survival not affected by underlying disease), ultimately fatal (not expected to survive more than five years) and rapidly fatal (not expected to survive more than one year) .
The incidence rate was calculated as the number of cases per 10,000 patient bed days, according to the studies conducted by the ESCMID .
Outbreak control measures
Laboratory diagnosis of Clostridium difficileinfection
The fecal toxin was determined using the Vidas C. difficile Tox A and B assay (bioMérieux, Marcy-l’Etoile, France).
Clostridium difficileculture and strains’ characterization
From 1st July 2012, diarrheal faecal samples of patients hospitalized in different affected wards, with a positive toxin test, were sent to the NIH reference laboratory.
The faecal samples were cultured, after ethanol shock, on the chromogenic medium chromID® C. difficile (bioMérieux, Marcy l’Etoile, France), under anaerobic conditions. C. difficile-suspected colonies were obtained from all patients, which were identified with the Rapid ID32 A galleries (bioMérieux), and confirmed by detection of gluD gene, which encodes the glutamate dehydrogenase specific for C. difficile.
The isolates were further characterized by capillary gel electrophoresis-based PCR ribotyping, as well as by PCR detection of enterotoxin A and cytotoxin B (tcdA and tdcB genes, respectively), binary toxin (cdtA and cdtB genes), and the deletion in the tcdC gene [21, 22]. The susceptibility to moxifloxacin was determined by E-Test® (bioMérieux).
Non normally distributed variables were compared with the Mann–Whitney test. Proportions were compared with the χ2 test. The P values of <0.05 were considered significant.
From 1st January to 31th December 2012, a total of 53 inpatients met the case definition of C. difficile-associated infection.
Characteristics of the 53 patients with Clostridium difficile -associated diarrhea during the outbreak period
Patients (n = 53) characteristics
Median age in years (interquartile range)
Place of origin
Long term care facility
Hospital admission during the 6 previous months
Antimicrobial exposure within 3-months before CDI diagnosis
Gastric acid suppressors (Proton pump inhibitors) intake within 3-months before CDI diagnosis
Death at 6 months
CDI Attributable cause
CDI Contributing cause
Fifty patients (94.3%) had a positive toxin assay; of these, 6.0% (three patients) had at least one negative fecal toxin test in the seven days preceding the positive test, and 18.0% (nine patients) in the preceding 30 days. For these patients, the toxin test was repeated several times due to the persistent diarrhea. For the remaining three patients, CDI was diagnosed by means of detection of pseudomembranous colitis by fibrosigmoidoscopy and biopsy, since two of them had ileus, and the third one had a negative toxin test, but a high clinical suspicion . The average time between patient’s admission and CDI diagnosis was 17 days, varying from one day to a maximum of 72 days.
According to patient’s hospital medical records, 47 (88.7%) of the 53 cases had received antibiotics in the three months prior to the diagnosis, and among these, all except one had received two or more classes of antibiotics. The most commonly used antibiotics were fluoroquinolones (mainly ciprofloxacin) and penicillins in association with clavulanic acid or tazobactam (59.6% each), cephalosporins (36.2%), aminoglicosides (31.9%) and carbapenems (23.4%). In addition, in the same period of time, 49 (92.5%) patients had been prescribed with proton pump inhibitors.
Concerning the CDI onset, cases were classified according to standardized published definitions [12, 13], and were distributed as follows: 30 (56.6%) cases were healthcare facility onset-healthcare facility associated (HCFA), 12 (22.6%) were community onset-HCFA, four (7.5%) cases were community onset, and seven (13.2%) cases had an indeterminate onset.
Incidence rates and epidemic curve
Treatment and clinical outcome
The global mortality within 30 days of the diagnosis of CDI was 39.6% (21/53) and further 13 patients died during the following 6 months, with a total crude mortality of 64.2% (34/53). A total of 24 patients (45.3%) died during the first CDI episode. From the 29 patients who survived the first episode, 12 relapsed (41.4%; 22.6% overall relapse rate) and 10 of these eventually died. After two independently clinical judgments, CDI was considered the attributable cause of death in six (17.6%; 11.3% overall attributable death) patients, a contributing cause in 19 (55.9%) patients and unrelated in nine (26.5%) cases.
The treatment of infected patients was established according to CHA protocol, which is based in the ESCMID guidelines : 48 patients were treated either with metronidazole (n = 18) or vancomycin (n = 19), or with both vancomycin and metronidazole (n = 11); five patients did not receive treatment (four died before they could start any treatment, and one patient with mild disease was dismissed and CDI resolved without treatment).
Death rate during the first episode of CDI was higher in the group of patients treated with vancomycin only (9/19, 47.4%), followed by the patients treated with both antibiotics (5/11, 45.5%) and by those treated with metronidazole only (6/18, 33.3%).
In order to evaluate the CDI clinical burden, we compared the 25 cases for which CDI was the direct cause of (or contributed to) the death (group 1), with the cases for which no death occurred (group 2) (n = 19). The median age of patients in group 1 was significantly higher than that of group 2 (80.2 years; interquartile range 76 to 87 years versus 74.2 years; interquartile range 67 to 81 years; P = 0.022). Other traits that differentiated these two groups of patients concerned the severity of the underlying clinical situation, as well as the markers of severe CDI, such as renal failure and leukocytosis . Indeed, taking into account the McCabe score, the rapidly fatal condition was observed in 56.0% (n = 14) of the patients of group 1, against 26.3% (n = 5) of the group 2 (P = 0.025). Also, both leukocytosis (19/25, 76% versus 6/19, 31.6%; P = 0.008) and renal failure (9/25; 36% versus 5/19; 26.3%; P > 0.05) were more frequent among group 1 than among group 2. As previously mentioned, in some cases, there was a considerable delay between patient’s admission and CDI diagnosis. Analyzing the two groups concerning this parameter, we observed that the average time for group 1 was 13.5 days (SD: 17.3) while for group 2 was 8.3 days (SD: 9.3), although this difference was not statistically significant.
Among the 22 samples that were sent to the NIH reference laboratory for culture and typing, 20 had a positive faecal culture for C. difficile. Regarding the ribotype, 16 (72.7%) belonged to ribotype 027, and the remaining four strains belonged to one of each ribotype, AI-84, 056, 020 and 014. All the studied isolates belonging to ribotype 027 were detected from HCFA cases, while the isolates from ribotypes 014 and AI-84 corresponded to indeterminate onset cases.
All the isolates were positive for both the enterotoxin A and the cytotoxin B encoding genes. Binary toxin gene and the 18-bp deletion in the tcdC gene were present in the all 027 isolates and absent in the other ribotypes.
The in vitro resistance to moxifloxacin was confirmed for all 16 isolates of the 027 ribotype, (minimum inhibitory concentration (MIC) ≥32 mg/L), while the other non-027 ribotype isolates were susceptible to this antibiotic (MICs of 0.5 and 0.75 mg/L).
To date, little information is available concerning C. difficile infection in Portugal. The few existing studies were published in non-indexed national journals, and report data on hospital casuistic. Nevertheless, they showed an important increase on CDI cases, as well on mortality rates, over the last five years. None of these studies identified the infecting strain.
The C. difficile 027 ribotype strain implicated in this outbreak showed traits that are common to the so called hypervirulent strains of this ribotype, including the known mutation in the tcdC gene and the presence of the binary toxin. Also relevant is the reduced susceptibility to fluoroquinolones of these strains, which likely contributes to their rapid dissemination  compared to non-outbreak isolates of the same type. Indeed, several studies point to fluoroquinolones therapy as a risk factor for C. difficile-associated disease during outbreaks [16, 26–28]. In the present cohort, 60% of the patients had a history of recent fluoroquinolones consumption, which has likely contributed to the spread of the epidemic strain, once it was introduced in the hospital.
The antibiotic stewardship implemented after the outbreak was declared, with special focus on reduced prescriptions of fluoroquinolones and third generation cephalosporins (Figure 1), presumably contributed to the progressive reduction in the incidence of CDI (Figure 4). This fact highlights the value of this measure to control nosocomial CDI, in agreement with previously published studies [16, 29, 30].
It was not possible to trace how this epidemic ribotype 027 C. difficile strain was introduced in the hospital, as the first samples were not sent to the NIH for ribotyping, hampering the identification of the index case. Moreover we were not able to associate the presence of this strain with the occurrence of other CDI cases in Portugal (outbreak or sporadic cases) probably due to the absence of a functional notification system of this disease. Nevertheless, it is worthwhile to underline the implication of C. difficile 027 strain in the present outbreak, in contrast to the declining trend reported across Europe concerning the prevalence of this strain in favor of another emergent ribotypes .
To date there is no consensus regarding the association of C. difficile ribotype 027 strains with more serious clinical outcome. Indeed, while most of the hospitals with 027 strain outbreaks have reported increased severity of the associated-disease, others are showing no difference to other ribotypes [16, 31, 32].
In the outbreak here described, 68% of the patients had at least one criterion of severe disease at diagnosis. Also, mortality occurred at high rate, with a 30-day all-cause mortality of 39.6% and a 027-CDI-related mortality rate of 18%, which is considerable higher than that reported in other 027-associated outbreaks [16, 33–36]. Nevertheless, our data suggests that, besides the infecting strain and its ability for rapid spreading when antibiotic pressure is present, other factors have contributed to this dramatic mortality rate such as advanced age, co-morbidities and the co-existence of rapidly fatal conditions.
Following antibiotic treatment for CDI, the highest mortality rate occurred for the vancomycin-treated patients, followed by the patients treated with vancomycin plus metronidazole, and the highest recurrence rate was also observed for these patients, which was an unexpected finding . The rationale for observing higher rates of treatment failure with vancomycin is not clear, but can be probably attributed to the fact that those patients had already a very poor prognostic, as also pointed by Gravel et al.. Also, the worse outcome for patients treated with vancomycin or both vancomycin and metronidazole may be due to the clinical severity of CDI which led to the choice of the treatment according to published guidelines .
It is worth mentioning that we did not evaluate which patients had received non-CDI antimicrobials during and/or after C. difficile treatment, as well as differences in antibiotic regimens. This likely implicated the disease outcome, as previous highlighted .
Concerning the CDI onset classification, we point out that patients classified as being CDI community onset, all came from nursing homes or other long term care facilities, so all CDI cases should be considered healthcare associated.
Aiming to control the outbreak, the ICT conducted an investigation that identified several problems concerning CDI patients, namely: previous and continuous excess use of broad spectrum antibiotics and proton pump inhibitors, with no clear indication registered in the clinical process; low level of clinical suspicion; and low sensibility of the diagnostic test used, contributing to late diagnosis of CDI, and consequently to a delay in the establishment of therapy and to a late implementation of control measures. In this context, it is worth emphasizing that the laboratory diagnosis of CDI was performed with a stand-alone rapid enzyme immunoassay for the C. difficile toxins, which has originated a number of false negative results, hampering the management of the CDI . In addition, at the beginning of the outbreak investigation, the OCT detected that in some cases clinicians did not registered severity criteria of CDI. Notably the presence of renal failure and leukocytosis were not always adequately valorized. A recent study showed that both these clinical conditions are predictors of severe CDI if measured on the day of diagnosis . To correct these findings, a protocol for the initial evaluation and treatment of all patients with diarrhea was implemented since May 2012.
It should also be pointed out the lack of awareness and knowledge of the institutional guidelines by the hospital staff, especially among clinicians, indicating the need of a different approach to motivate HCW in infection control educational activities, which are not mandatory in Portugal. The bundle of measures that were taken tried to face and correct the detected problems, together with the involvement of all HCW in their resolution.
We therefore considered that all these constraints, together with the presence of a hypervirulent strain with a high spread capacity, contributed to the increased risk of nosocomial transmission and to the severity of CDI episodes observed in this outbreak.
In conclusion, regarding the infecting strain, while the high potential for spreading of 027 strains seems clear, further investigation on epidemic strains can be a useful contribution to shed some light on the virulence and epidemic potential of C. difficile 027, in accordance with recent studies suggesting that subtypes may influence the severity of clinical outcome.
High level suspicion and early diagnosis of CDI, with initial correct evaluation of severity criteria, and adequate treatment according to this evaluation, together with the reinforcement of a bundle of infection control measures is necessary to avoid the spread of C. difficile. Antibiotic stewardship is perhaps the most important isolated measure that can be taken to prevent CDI related clinical burden, but it is difficult to accomplish in a growing population of elderly, dependent and institutionalized patients with consecutive healthcare associated infections.
Finally, this is the first outbreak of C. difficile due to ribotype 027 strain that is reported in Portugal, highlighting the importance of implementing a close surveillance of CDI in Portugal, both at population-based and nosocomial levels.
We thank Dr. Lourdes Cabezuelo for revising the death causes and Dr Raquel Pinho for acquisition of data. We also thank Sónia Domingos for database construction. Finally, we thank Dr Luis Alcalá for critically reviewing the paper.
- McDonald L, Owings M, Jernigan D: Clostridium difficile infection in patients discharged from US short stay hospitals, 1996–2003. Emerg Infect Dis. 2006, 12 (3): 409-415. 10.3201/eid1205.051064.View ArticlePubMedPubMed CentralGoogle Scholar
- Bignardi G: Risk factors for Clostridium difficile infection. J Hosp Infect. 1998, 40 (1): 1-15. 10.1016/S0195-6701(98)90019-6.View ArticlePubMedGoogle Scholar
- O'Connor J, Johnson S, Gerding D: Clostridium difficile infection caused by the epidemic BI/NAP1/027 strain. Gastroenterology. 2009, 136 (6): 1913-1924. 10.1053/j.gastro.2009.02.073.View ArticlePubMedGoogle Scholar
- Di Bella S, Musso M, Cataldo M, Meledandri M, Bordi E, Capozzi D, Cava M, Chiaradonna P, Prignano G, Petrosillo N: Clostridium difficile infection in Italian urban hospitals. Data from 2006 through 2011. BMC Infect Dis. 2013, 13 (1): 146-10.1186/1471-2334-13-146.View ArticlePubMedPubMed CentralGoogle Scholar
- Wiegand P, Nathwani D, Wilcox M, Stephens J, Shelbaya A, Haider S: Clinical and economic burden of Clostridium difficile infection in Europe: a systematic review of healthcare-facility-acquired infection. J Hosp Infect. 2012, 81 (1): 1-14. 10.1016/j.jhin.2012.02.004.View ArticlePubMedGoogle Scholar
- Rupnik M, Wilcox M, Gerding D: Clostridium difficile infection: new developments in epidemiology and pathogenesis. Nat Rev Microbiol. 2009, 7 (7): 526-536. 10.1038/nrmicro2164.View ArticlePubMedGoogle Scholar
- Kuijper E, Coignard B, Tüll P, difficile; ESGfC, States; EM, Control. ECfDPa: Emergence of Clostridium difficile-associated disease in North America and Europe. Clin Microbiol Infect. 2006, 12 (Suppl 6): 2-18.View ArticlePubMedGoogle Scholar
- Carter G, Douce G, Govind R, Howarth P, Mackin KE, Spencer J, Buckley A, Antunes A, Kotsanas D, Jenkin G, Dupuy B, Rood J, Lyras D: The anti-sigma factor TcdC modulates hypervirulence in an epidemic BI/NAP1/027 clinical isolate of Clostridium difficile. PLoS Pathog. 2011, 7 (10): e1002317-10.1371/journal.ppat.1002317.View ArticlePubMedPubMed CentralGoogle Scholar
- Cartman S, Heap J, Kuehne S, Cockayne A, Minton N: The emergence of 'hypervirulence' in Clostridium difficile. Int J Med Microbiol. 2010, 300 (6): 387-395. 10.1016/j.ijmm.2010.04.008.View ArticlePubMedGoogle Scholar
- Bauer M, Notermans D, van Benthem B, Brazier J, Wilcox M, Rupnik M, Monnet D, van Dissel J, Kuijper E, Group ES: Clostridium difficile infection in Europe: a hospital-based survey. Lancet. 2011, 377 (9759): 63-73. 10.1016/S0140-6736(10)61266-4.View ArticlePubMedGoogle Scholar
- Bauer M, Kuijper E, van Dissel JT: European Society of Clinical Microbiology and Infectious Diseases (ESCMID): treatment guidance document for Clostridium difficile infection (CDI). Clin Microbiol Infect. 2009, 15 (12): 1067-1079. 10.1111/j.1469-0691.2009.03099.x.View ArticlePubMedGoogle Scholar
- Cohen S, Gerding D, Johnson S, Kelly C, Loo V, McDonald L, Pepin J, Wilcox M, Society for Healthcare Epidemiology of America; Infectious Diseases Society of America: Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the infectious diseases society of America (IDSA). Infect Control Hosp Epidemiol. 2010, 31 (5): 431-455. 10.1086/651706.View ArticlePubMedGoogle Scholar
- Surawicz C, Brandt L, Binion D, Ananthakrishnan A, Curry S, Gilligan P, McFarland L, Mellow M, Zuckerbraun B: Guidelines for diagnosis, treatment, and prevention of Clostridium difficile infections. Am J Gastroenterol. 2013, 108 (4): 478-498. 10.1038/ajg.2013.4.View ArticlePubMedGoogle Scholar
- McCabe W, Jackson G: Gram-negative bacteremia: I. etiology and ecology. Arch Intern Med. 1962, 110: 847-855. 10.1001/archinte.1962.03620240029006.View ArticleGoogle Scholar
- Center for Disease Control and Prevention: Vital signs: preventing Clostridium difficile infections. MMWR. 2012, 61 (09): 157-162.Google Scholar
- Aldeyab M, Devine M, Flanagan P, Mannion M, Craig A, Scott M, Harbarth S, Vernaz N, Davies E, Brazier J, Smyth B, McElnay J, Gilmore B, Conlon G, Magee FA, Elhajji F, Small S, Edwards C, Funston C, Kearney M: Multihospital outbreak of Clostridium difficile ribotype 027 infection: epidemiology and analysis of control measures. Infect Control Hosp Epidemiol. 2011, 32 (3): 210-219. 10.1086/658333.View ArticlePubMedGoogle Scholar
- Vonberg R, Kuijper E, Wilcox M, Barbut F, Tüll P, Gastmeier P, van den Broek P, Colville A, Coignard B, Daha T, Debast S, Duerden B, van den Hof S, van der Kooi T, Maarleveld H, Nagy E, Notermans D, O'Driscoll J, Patel B, Stone S, Wiuff C, European C difficile-Infection Control Group; European Centre for Disease Prevention and Control (ECDC): Infection control measures to limit the spread of Clostridium difficile. Clin Microbiol Infect. 2008, 14 (Suppl 5): 2-20.View ArticlePubMedGoogle Scholar
- Carrico R, Archibald L, Bryant K, Dubberke E, Fauerbach L, Garcia J, Gould C, Koll B, Mayfield J, Pang X, Ramirez J, Stephens D, Stricof R, Wiemken T: Guide to the Elimination of Clostridium difficile in Healthcare Settings. 2008, Washington DC: APICGoogle Scholar
- Debast S, Vaessen N, Choudry A, Wiegers-Ligtvoet E, van den Berg R, Kuijper E: Successful combat of an outbreak due to Clostridium difficile PCR ribotype 027 and recognition of specific risk factors. Clin Microbiol Infect. 2009, 15 (5): 427-434. 10.1111/j.1469-0691.2009.02713.x.View ArticlePubMedGoogle Scholar
- Carman R, Wickham K, Chen L, Lawrence A, Boone J, Wilkins T, Kerkering T, Lyerly D: Glutamate dehydrogenase is highly conserved among Clostridium difficile ribotypes. J Clin Microbiol. 2012, 50 (4): 1425-1426. 10.1128/JCM.05600-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Indra A, Huhulescu S, Schneeweis M, Hasenberger P, Kernbichler S, Fiedler A, Wewalka G, Allerberger F, Kuijper E: Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol. 2008, 57 (Pt 11): 1377-1382.View ArticlePubMedPubMed CentralGoogle Scholar
- Persson S, Jensen J, Olsen K: Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. J Clin Microbiol. 2011, 49 (12): 4299-4300. 10.1128/JCM.05161-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Debast S, Bauer M, Kuijper E: Committee: European Society of Clinical Microbiology and Infectious Diseases: update of the treatment guidance document for Clostridium difficile infection. Clin Microbiol Infect. 2014, 20 (Suppl 2): 1-26.View ArticlePubMedGoogle Scholar
- Bauer M, Hensgens M, Miller M, Gerding D, Wilcox M, Dale A, Fawley W, Kuijper E, Gorbach S: Renal failure and leukocytosis are predictors of a complicated course of Clostridium difficile infection if measured on day of diagnosis. Clin Infect Dis. 2012, 55 (Suppl 2): S149-S153. 10.1093/cid/cis340.View ArticlePubMedPubMed CentralGoogle Scholar
- Vardakas K, Konstantelias A, Loizidis G, Rafailidis P, Falagas M: Risk factors for development of Clostridium difficile infection due to BI/NAP1/027 strain: a meta-analysis. Int J Infect Dis. 2012, 16 (11): e768-e773. 10.1016/j.ijid.2012.07.010.View ArticlePubMedGoogle Scholar
- Gaynes R, Rimland D, Killum E, Lowery H, Johnson T, Killgore G, Tenover F: Outbreak of Clostridium difficile infection in a long-term care facility: association with gatifloxacin use. Clin Infect Dis. 2004, 38 (5): 640-645. 10.1086/381551.View ArticlePubMedGoogle Scholar
- Pépin J, Saheb N, Coulombe M, Alary M, Corriveau M, Authier S, Leblanc M, Rivard G, Bettez M, Primeau V, Nguyen M, Jacob C, Lanthier L: Emergence of fluoroquinolones as the predominant risk factor for Clostridium difficile-associated diarrhea: a cohort study during an epidemic in Quebec. Clin Infect Dis. 2005, 41 (9): 1254-1260. 10.1086/496986.View ArticlePubMedGoogle Scholar
- Biller P, Shank B, Lind L, Brennan M, Tkatch L, Killgore G, Thompson A, McDonald L: Moxifloxacin therapy as a risk factor for Clostridium difficile-associated disease during an outbreak: attempts to control a new epidemic strain. Infect Control Hosp Epidemiol. 2007, 28 (2): 198-201. 10.1086/511789.View ArticlePubMedGoogle Scholar
- Muto C, Blank M, Marsh J, Vergis E, O'Leary M, Shutt K, Pasculle A, Pokrywka M, Garcia JG, Posey K, Roberts TL, Potoski B, Blank G, Simmons R, Veldkamp P, Harrison L, Paterson D: Control of an outbreak of infection with the hypervirulent Clostridium difficile BI strain in a university hospital using a comprehensive "bundle" approach. Clin Infect Dis. 2007, 45 (10): 1266-1273. 10.1086/522654.View ArticlePubMedGoogle Scholar
- Kallen A, Thompson A, Ristaino P, Chapman L, Nicholson A, Sim B, Lessa F, Sharapov U, Fadden E, Boehler R, Gould C, Limbago B, Blythe D, McDonald L: Complete restriction of fluoroquinolone use to control an outbreak of Clostridium difficile infection at a community hospital. Infect Control Hosp Epidemiol. 2009, 30 (3): 264-272. 10.1086/595694.View ArticlePubMedGoogle Scholar
- Morgan O, Rodrigues B, Elston T, Verlander N, Brown D, Brazier J, Reacher M: Clinical severity of Clostridium difficile PCR ribotype 027: a case-case study. PLoS One. 2008, 3 (3): e1812-10.1371/journal.pone.0001812.View ArticlePubMedPubMed CentralGoogle Scholar
- Inns T, Gorton R, Berrington A, Sails A, Lamagni T, Collins J, Perry J, Hill K, Magee J, Gould K: Effect of ribotype on all-cause mortality following Clostridium difficile infection. J Hosp Infect. 2013, 84 (3): 235-241. 10.1016/j.jhin.2013.04.008.View ArticlePubMedGoogle Scholar
- Loo V, Poirier L, Miller M, Oughton M, Libman M, Michaud S, Bourgault A, Nguyen T, Frenette C, Kelly M, Vibien A, Brassard P, Fenn S, Dewar K, Hudson T, Horn R, René P, Monczak Y, Dascal A: A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N Engl J Med. 2005, 353 (23): 2442-2449. 10.1056/NEJMoa051639.View ArticlePubMedGoogle Scholar
- Kuijper E, Barbut F, Brazier J, Kleinkauf N, Eckmanns T, Lambert M, Drudy D, Fitzpatrick F, Wiuff C, Brown D, Coia J, Pituch H, Reichert P, Even J, Mossong J, Widmer A, Olsen K, Allerberger F, Notermans D, Delmée M, Coignard B, Wilcox M, Patel B, Frei R, Nagy E, Bouza E, Marin M, Akerlund T, Virolainen-Julkunen A, Lyytikäinen O, et al: Update of Clostridium difficile infection due to PCR ribotype 027 in Europe, 2008. Euro Surveill. 2008, 13 (31): 18942-PubMedGoogle Scholar
- Kelly C, LaMont J: Clostridium difficile-more difficult than ever. N Engl J Med. 2008, 359 (18): 1932-1940. 10.1056/NEJMra0707500.View ArticlePubMedGoogle Scholar
- Birgand G, Blanckaert K, Carbonne A, Coignard B, Barbut F, Eckert C, Grandbastien B, Kadi Z, Astagneau P: Investigation of a large outbreak of Clostridium difficile PCR-ribotype 027 infections in northern France, 2006–2007 and associated clusters in 2008–2009. Euro Surveill. 2010, 15 (25): 19597-PubMedGoogle Scholar
- Bouza E: Clostridium difficile infection: same incidence and worse prognosis?. Clin Infect Dis. 2009, 48 (5): 577-579. 10.1086/596704.View ArticlePubMedGoogle Scholar
- Gravel D, Miller M, Simor A, Taylor G, Gardam M, McGeer A, Hutchinson J, Moore D, Kelly S, Boyd D, Mulvey M: Health Care–Associated Clostridium difficile Infection in Adults Admitted to Acute Care Hospitals in Canada: A Canadian Nosocomial Infection Surveillance Program Study. Clin Infect Dis. 2009, 48 (5): 568-576. 10.1086/596703.View ArticlePubMedGoogle Scholar
- Shaughnessy M, Amundson W, Kuskowski M, DeCarolis D, Johnson J, Drekonja D: Unnecessary antimicrobial use in patients with current or recent Clostridium difficile infection. Infect Control Hosp Epidemiol. 2013, 34 (2): 109-116. 10.1086/669089.View ArticlePubMedGoogle Scholar
- Wilcox M: Overcoming barriers to effective recognition and diagnosis of Clostridium difficile infection. Clin Microbiol Infect. 2012, 18 (Suppl 6): 13-20.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/209/prepub
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