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Table 1 Results of real-time PCR, culture identification and antimicrobial susceptibility of positive samples from BC bottles

From: Diagnosis by real-time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections

Patients Date samples Bactec® Results Bacterial isolated from blood culture (Phoenix®) Bacterial detected with the PCR test Antimicrobial susceptibility (Phoenix®) Resistance genes by real-time PCR
  10/9/2009 Positive S. epidermidis CoNS S* Oxacilin mecA
10/9/2009 Positive S. epidermidis CoNS S Oxacilin mecA
10/9/2009 Positive S. epidermidis CoNS S Oxacilin mecA
      Cephalosporins  
01 12/20/2009 Positive C.freundii CoNS S and ND
      Carbapenems  
12/20/2009 Positive S. epidermidis CoNS R* Oxacilin mecA
3/1/2009 Negative ND CoNS and GN NA NA blaSHV
1/9/2010 Negative ND Aspergillus spp. NA NA NA
12/4/2008 Positive E. cloacae E. cloacae R Cephalosporins ND
02 12/4/2008 Positive E. cloacae E. cloacae R Cephalosporins ND
12/4/2008 Positive E. cloacae E. cloacae R Cephalosporins blaSHV
3/5/2009 Positive CoNS CoNS R Oxacilin mecA
03 3/5/2009 Positive CoNS CoNS R Oxacilin mecA
3/5/2009 Positive CoNS CoNS R Oxacilin ND
5/8/2009 Positive P. putida ND NA NA ND
04 5/8/2009 Positive S. epidermidis CoNS R Oxacilin mecA
5/8/2009 Positive S. epidermidis CoNS R Oxacilin mecA
05 8/2/2009 Positive S. pneumoniae ND S Penicilin ND
8/2/2009 Positive S. pneumoniae S. pneumoniae S Penicilin ND
6/6/2009 Positive E. faecium Enterococcus spp. R Glycopeptide vanA
06 6/6/2009 Positive E. faecium ND R Glycopeptide vanA
6/6/2009 Positive E. faecium ND R Glycopeptide vanA
07 1/18/2009 Positive NFGNB A. baumannii R Cephalosporins and Carbapenems ND
      Cephalosporins  
1/31/2009 Positive Acinetobacter spp. ND R and ND
      Carbapenems  
1/31/2009 Negative ND A. baumannii NA NA ND
     R Cephalosporins  
08 3/2/2009 Positive K. pneumoniae ND and and ND
     S Carbapenems  
09 9/28/2009 Positive E. faecalis ND S Glycopeptide ND
9/28/2009 Positive E. faecalis Enterococcus spp. S Glycopeptide ND
10 12/12/2008 Positive E. faecium Enterococcus spp. R Glycopeptide vanA
12/9/2008 Negative ND Enterococcus spp. NA NA ND
11 12/18/2008 Negative ND K. pneumoniae NA NA blaCTX-M
12/21/2008 Negative ND K. pneumoniae NA NA blaCTX-M
2/21/2009 Negative ND CoNS and GN NA NA ND
28/03/2011 Negative ND CoNS NA NA ND
2/21/2009 Negative ND Candida spp. NA NA NA
12 4/8/2011 Positive S. epidermidis CoNS R Oxacilin mecA
4/8/2011 Positive S. epidermidis CoNS R Oxacilin mecA
4/8/2011 Positive S. epidermidis CoNS R Oxacilin mecA
13 3/14/2009 Negative ND ND NA NA blaSHV
14 11/4/2010 Positive A. baumannii A. baumannii S Cephalosporins ND
15 3/26/2011 Positive E. coli E. coli S Cephalosporins and Carbapenems ND
16 3/31/2011 Positive S. epidermidis CoNS R Oxacilin mecA
17 5/8/2009 Positive CoNS ND R Oxacilin ND
5/8/2009 Positive CoNS ND R Oxacilin ND
18 5/14/2009 Negative ND Aspergillus spp. NA NA NA
19 5/2/2009 Negative ND Candida albicans NA NA NA
5/4/2009 Negative ND Candida albicans NA NA NA
  1. R: Resistant; S: Susceptible; * CLSI 2010; NA: results not available; ND: Not Detected.