Effect of Ascaris Lumbricoides specific IgE on tuberculin skin test responses in children in a high-burden setting: a cross-sectional community-based study

Infectious diseases are important causes of morbidity and mortality in children living in the developing world. Tuberculosis is a major global threat with an estimated 9.4 million incident cases reported globally in 2009 [1], with South Africa ranked third with an estimated annual notification rate of 971 per 100 000 per year [1]. Paediatric tuberculosis contributes an estimated 15-20% of the overall global caseload, with >80% cases notified from the 22 so-called “high-burden” countries, including South Africa [2]. Young children exposed to and infected with Mycobacterium tuberculosis (M.tb) are at high risk for disease progression in the absence of effective chemoprophylaxis [3].

Poor socio-economic circumstances, prevalent in many settings with a high burden of tuberculosis, also predispose to poor living conditions, overcrowding and increased risk of helminth exposure and infection [4, 5]. Helminth infection in children is often chronic and is associated with anaemia, growth retardation, impaired cognitive functioning and chronic inflammatory diseases [6–8]. The World Health Organization (WHO) classifies South Africa as one of the 130 helminth-endemic countries (prevalence of infection > 20% - 50%) [9]. The WHO Integrated Management of Childhood Illness (IMCI) guidelines therefore routinely recommend regular deworming in children older than 1 year [10]; however, this is not consistently practiced and the constant risk of re-infection remains high in helminth-prevalent settings [4, 5].

M.tb exposure involves complex immune processes and leads to a spectrum of infection and clinical disease outcomes in children, including M.tb-exposed but uninfected, infected non-diseased and diseased [11, 12]. In addition to innate host immunity, protection against M.tb requires an effective adaptive cellular immune response characterized by robust T helper cell type 1 (Th1) T-cell immunity and relative weaker T helper cell type 2 (Th2) T-cell immune responses [13–17]. The tuberculin skin test (TST), the most established clinical-epidemiological immune measure of M.tb infection, is a marker of a Th1-type delayed type hypersensitivity reaction. Previously M.tb-sensitized circulating lymphocytes are attracted to the intra-dermal tuberculin injection site, followed by monocyte, macrophage, CD4 + − and CD8+ T-lymphocyte accumulation and release of inflammatory mediators that causes clinical erythema and oedema [12].

Th2 cells produce cytokines which play a role in protection against extracellular organisms and are increased in the presence of helminth infection [18–21], specifically Ascaris lumbricoides [20] and hookworm species [20, 21]. Data regarding the role of Th2 cells in the presence of Trichiuris trichiura infection are inconsistent [15, 22]. Th2 cell types impair signal transduction and induce anergy of immune cells, which might decrease the ability to generate protective cellular immunity to protect against common infections, like M.tb and to routine vaccinations [19]. Helminth infection is further associated with suppressive T-cell population induction and inhibitory cytokine production which can suppress Th1-type responses and interfere with effector T-cell activation, potentially resulting in altered memory immune responses against M.tb [15].

The current limited evidence supports a potential immune modulating effect of helminths on mycobacterial immune responses in humans [15, 21, 23], although contradictory evidence also exists [24, 25]. We therefore investigated the effect of an Ascaris-specific IgE positive status on a commonly used marker of M.tb infection (TST), in children in a setting with a high burden of tuberculosis. We hypothesized primarily that helminth infection in children, while controlling for M.tb exposure and other relevant covariates, would decrease the ability to generate an appropriate Th1 immune response characterized by a positive TST in children.

This was a prospective cross-sectional community-based study. Study participants were recruited from three high burden tuberculosis communities: Ravensmead, Uitsig (R/U) and Site C, Khayelitsha (Site C), in Cape Town, Western Cape Province, South Africa. The communities are demographically heterogeneous, and respectively represent ethnic groups that are predominantly of South African Mixed (R/U) and Xhosa African ancestry (Site C).

The tuberculosis notification rate in the province was 994 per 100 000 in adults and 671 per 100 000 in children aged 0 – 14 years in 2008 (Western Cape Department of Health, unpublished data). There is near universal infant bacille Calmette-Guérin (BCG) vaccination.

Prior surveys from school-going children in R/U found that 25% were stool positive for Ascaris lumbricoides and 51% for Trichuris trichiura [26]. Routine clinic and school-based deworming has since become standard of care in local health care centres. There are currently no published data on the prevalence of helminth infection in children in Khayelitsha.

Participants were recruited following identification of consenting adults (≥ 18 years of age), who routinely started tuberculosis treatment in the preceding 3 months (“source case”) at the local clinic. Participants were eligible for enrolment if they were a household child contact between 3 months and 15 years of age. Households were identified through TB source cases (“tuberculosis households”), and matched neighbouring households without known tuberculosis exposure (“neighbouring households”). The ratio between tuberculosis and neighbouring households was approximately 1:1.

We identified tuberculosis households within 2–4 weeks following the adult source case identification, then completed a home visit and collected data on M.tb exposure and demographic characteristics. In addition, all adult household members were screened for symptoms of tuberculosis followed by sputum collection, if they were symptomatic.

Following written informed consent from the parents/caregivers, children aged 3 months – 15 years who were not currently on tuberculosis therapy, were enrolled in the study. Children were deferred for enrolment if there was a) prior TST administration in the preceding 3 months, b) live measles or polio vaccine within the past 6 weeks or c) recent acute illness requiring hospitalization (preceding 6 weeks). Children on isoniazid preventive therapy (IPT) were not excluded, whereas HIV-infected children were.

All children were investigated for M.tb infection and disease using standard protocols [27, 28], including TST, a standard symptom questionnaire , blinded independent dual expert review of chest radiographs (postero-anterior and lateral), sputum or gastric washing for mycobacterial culture (Mycobacterial Growth Indicator Tubes; Becton Dickinson, Sparks, USA), a TST, and clinical examination.

The TST (Mantoux method; 2 tuberculin units purified protein derivative; Statens Serum Institute, Copenhagen) was administered intra-dermally on the volar aspect of the left forearm by trained standardized study nurses, and read within 48–72 hours using the ball-point and ruler method and callipers. The TST was regarded as positive if measuring ≥10 mm in HIV-negative children for clinical care. Unless already known to be HIV-infected, participants were tested for HIV (Abbott Determine Rapid HIV test), followed by a confirmatory laboratory based ELISA/PCR test if positive or indeterminate.

We also recorded information on BCG vaccination history and scar presence, previous and current isoniazid preventive therapy, previous tuberculosis treatment, nutritional status (z-scores), household composition, measures of socio-economic status (household assets), and reported history of deworming during the preceding 6 months. Z-scores were calculated with reference to the 2006 World Health Organization Child Growth Standards for children up to five years of age and the 2000 Centres for Disease Control and Prevention growth charts reference population for children that were older than 5 years [32]. Reported socio-economic indicators were based on surveys in our study communities and were used in the absence of reliable data on reported income, given high unemployment rates in these impoverished urban communities.

Of 337 total child participants enrolled, 271 HIV-negative children were included in the analysis (n = 66, 20% were HIV infected and excluded). The median age was 4 years (range: 4 months to 15 years).

Few children (2%) were receiving IPT at the time of enrolment despite the fact that 67% of children were household contacts of a tuberculosis index case currently on therapy. Of children with a documented contact (n = 181), 121 were below 5 years of age and were referred for IPT. Previous IPT use was reported in 9% and 3% had previously received tuberculosis treatment.

Ascaris IgE results are described in Table 2. A total of 65 (24%) children were Ascaris IgE positive. Of these, 37% were also TST positive. Most Ascaris IgE positive participants were within RAST range 2 (moderate level of allergen specific IgE) followed by ranges 1 (low level of allergen specific IgE) and 3 (high level of allergen specific IgE) [Table 2]. Of the 8% of children in whom the caregiver reported that the child had passed a worm in the preceding 3 months, most (86%) had been dewormed. Anthropometric measures were not associated with either positive Ascaris or TST status.

Based on the univariate analysis, we included age, M.tb contact score and binary Ascaris IgE status in a regression model that demonstrated an increased risk for a positive TST in older children (aOR 1.2, 95% CI 1.1 – 1.3) and those with a higher M.tb contact score (aOR 1.2, 95% CI 1.1 – 1.3). This model also showed an inverse association between TST and Ascaris IgE positivity (aOR 0.6, 95% CI 0.1 – 1.1).

We demonstrate a high prevalence of M.tb and Ascaris lumbricoides infection in children from households with and without documented tuberculosis exposure living in poor urban South African communities. Our results suggest an inverse relationship between Ascaris IgE and TST positive status, which is consistent with our hypothesis.

False-negative TST results might be due to technical factors or a suppressed immune status while false-positive tests may be attributed to BCG vaccination and the effect of non-tuberculous mycobacteria [12]. In our study, a positive TST was associated with increasing age and an increasing M.tb contact score, which is biologically plausible and consistent with other data [30, 34]. The presence of a BCG vaccination scar [35–37] and indicators of higher socio-economic household status [38] protected against having a positive M.tb infection phenotype.

Our multivariable logistic regression adjusting for age, degree of M.tb exposure and previous TB therapy, supports this hypothesis; children with a positive Ascaris IgE appeared to be less likely to mount a positive TST response, especially if they were younger.

In the absence of stool samples, as in this study, serum Ascaris-induced specific IgE is widely used as proxy for Ascaris infection. Ascaris IgE however cannot differentiate previous infection from new infection and/or previous sensitization, and therefore assumes Ascaris infection and Th2 polarization without information regarding the time of infection [39], similar to measures of M.tb infection. Additionally potential cross-reactivity due to Trichiuris trichiura infection cannot be excluded [40]. Despite these limitations, Ascaris IgE responses remain a useful proxy for immunological and epidemiological studies, and are a standard and widely used indication of Ascaris exposure/infections.

Despite universal clinic access to deworming and school- based deworming programs, the positive Ascaris IgE levels of 24%, although lower than previously reported from these communities [26], are high and consistent with WHO definitions for a helminth endemic country [9]. Deworming may not be practised as regularly as required (at least once a year), as indicated by the low proportion of caregivers (7%) who had reported deworming of the child in the preceding 3 months. On the other hand, helminth re-exposure and infection, given the poor socio-economic circumstances is also possible [9, 38]. These factors should be investigated in longitudinal studies with repeat measures of infection.

Older children (OR 1.2, p < 0.001) were more likely to be Ascaris positive which we attribute to the age-related risk of infection and immune system responsiveness, and possibly due to behavioural patterns (i.e. more outdoor activities in shared environments like schools and sports fields).

Few children were on IPT at enrolment which could reflect the active contact tracing strategy used by the study; however previous studies from these communities indicate poor IPT uptake and adherence in young children under routine programmatic conditions [41–43].

This study is limited by its cross-sectional nature, the limited sample size and the exclusion of HIV-infected children, due to small numbers. The enrolment strategy was purposeful to ensure a high proportion of children with recent M.tb exposure and included children from neighbouring “control” households as a measure of background M.tb transmission. Finally, the proxy used for Ascaris in our study does not apply to all potential helminth species, and might underestimate the general and differential effect of helminth infection. Deworming was only reported on a questionnaire basis and its impact on mycobacterial and other immune responses could therefore not be rigorously evaluated.

In light of these limitations, these results need to be verified in paediatric cohort studies, preferably with additional measures of helminth infection including stool samples and the standard evaluation of the effect of deworming on mycobacterial immune responses.

The results support our hypothesis that helminth infection may change immune responses in children. This may play a clinically measureable role in widely used immune diagnostic tests that measures M.tb infection, especially in high prevalence tuberculosis and helminth infection settings.