This article has Open Peer Review reports available.
Spatial clustering in the spatio-temporal dynamics of endemic cholera
© Ruiz-Moreno et al; licensee BioMed Central Ltd. 2010
Received: 14 April 2009
Accepted: 6 March 2010
Published: 6 March 2010
The spatio-temporal patterns of infectious diseases that are environmentally driven reflect the combined effects of transmission dynamics and environmental heterogeneity. They contain important information on different routes of transmission, including the role of environmental reservoirs. Consideration of the spatial component in infectious disease dynamics has led to insights on the propagation of fronts at the level of counties in rabies in the US, and the metapopulation behavior at the level of cities in childhood diseases such as measles in the UK, both at relatively coarse scales. As epidemiological data on individual infections become available, spatio-temporal patterns can be examined at higher resolutions.
The extensive spatio-temporal data set for cholera in Matlab, Bangladesh, maps the individual location of cases from 1983 to 2003. This unique record allows us to examine the spatial structure of cholera outbreaks, to address the role of primary transmission, occurring from an aquatic reservoir to the human host, and that of secondary transmission, involving a feedback between current and past levels of infection. We use Ripley's K and L indices and bootstrapping methods to evaluate the occurrence of spatial clustering in the cases during outbreaks using different temporal windows. The spatial location of cases was also confronted against the spatial location of water sources.
Spatial clustering of cholera cases was detected at different temporal and spatial scales. Cases relative to water sources also exhibit spatial clustering.
The clustering of cases supports an important role of secondary transmission in the dynamics of cholera epidemics in Matlab, Bangladesh. The spatial clustering of cases relative to water sources, and its timing, suggests an effective role of water reservoirs during the onset of cholera outbreaks. Once primary transmission has initiated an outbreak, secondary transmission takes over and plays a fundamental role in shaping the epidemics in this endemic area.
The spatial distribution of cases for infectious diseases that are environmentally driven, such as those of water-borne and vector-borne transmission, reflect the combined effect of environmental heterogeneity and disease dynamics. As such, spatial patterns can be used to examine hypotheses on routes of transmission at different scales [1–3]. For example, long-term disease data at the population level for multiple cities and towns, or presence-absence data in counties or districts, have provided numerous insights on patterns of propagation at the relatively large scales of countries and even transcontinental couplings [1, 4–6]. Detailed spatial records at the individual level have a long tradition at the interface of geography and epidemiology, with one of the best known examples found in the discovery of cholera's water source by Snow . However, these detailed data have been typically short-term and limited to particular epidemics. Longer-term data sets at the high-resolution of individuals but spanning multiple epidemics are becoming available, with the current interest in transmission networks and the interface of Geographic Information Systems and disease patterns. This type of data makes possible the description of how spatial patterns and clustering in particular, change in time, as a first step towards addressing the spatio-temporal dynamics of disease at a high resolution, as well as the coarse-graining of these dynamics, from small, individual, to large, population, levels.
In general, higher case numbers are expected in areas surrounding pathogen sources, and lower ones, in areas far away from these sources. For example, for vector-borne diseases, the clustering of cases might occur nearby the specific habitat of the vector, and for water-borne diseases, in the proximity of infected aquatic reservoirs. However, patterns are complicated by the fact that transmission can occur from both environmental reservoirs and previously infected individuals, reflecting the dynamics of transmission, human behavior and environmental variability. For cholera, an acute diarrheal infectious disease caused by the bacterium Vibrio cholerae, two routes of transmission have been proposed , the first one from aquatic reservoirs in the environment, and the second one, from previously infected individuals. To address the respective roles of these two routes of transmission, this paper examines the clustering patterns of cholera and how they vary in time with an extensive data set for a rural area of Bangladesh, covering twenty-one years and groups of households in the landscape.
Although the cholera bacterium has an enormous diversity with more than 200 serogroups classified based on the somatic O antigen, only two such groups, O1 and O139, are pathogenic [9–11]. V. cholerae O1 can be subdivided in turn into two biotypes (phenotypes), classical and El Tor . The O1 serogroup can also be subdivided into serotypes (based on antigenic responses) called Ogawa, Inaba and the extremely rare Hikojima . Moreover, the Bengal strain, V. cholerae O139, appeared in 1992  presumably by horizontal gene transfer between O1 and another O serogroup . Nowadays, evidence from field studies and epidemiological models, supports the fact that the classical variant is more infective than the El Tor variant. However, the El Tor variant is more resilient outside the human host, surviving in the environment for a longer period than the classical strain [14, 15]. Recent evidence suggests that V. cholerae O139, which exhibits no cross immunity with the O1 serogroups, performs better in both, the environment and the human gastrointestinal tract , raising questions on its short epidemic period.
Survivorship of the pathogens, availability of susceptible individuals and temporal immunity appear to be important processes shaping the pronounced seasonality of cholera. In non-endemic locations (like Peru, Brazil and several countries in Africa), epidemics are confined to the warm and rainy season. By contrast, in endemic areas in Bangladesh and former Bengal, two epidemic peaks occur each year corresponding to the warm seasons before and after the monsoonal rains [11, 17]. A full explanation of this complex seasonal pattern is still lacking, and a number of environmental and ecological drivers have been proposed (for example, the presence of environmental cholera phages, the degree of crowdedness and availability of susceptible individuals) . For estuarine regions of Bangladesh with two peaks per year, evidence supports a relationship between the initiation of the spring peak of cholera and favorable environmental conditions for the bacterial population, such as warmer temperatures . A dilution effect would then drive the reduction of cholera during the monsoonal season , although recent mathematical models of historical mortality time series, suggest an alternative explanation in which susceptibles are depleted by a large number of asymptomatics and short-term immunity . Finally, an increase in human-to-human contact, as a consequence of the post monsoonal floods might be an important factor in the second epidemic peak .
Infectivity and survivorship affects the spatial distribution of V. cholerae and its availability to colonize susceptible individuals. From this perspective, an initial study on the spatial occurrence of cholera cases by Glass and colleagues  provided a detailed description of cholera epidemics during the period from 1966 to 1980. This study identified villages with high and low cholera risk. Based on these findings, Miller and colleagues proposed a cholera model with two routes of transmission, primary and secondary, respectively . Primary transmission occurs as the result of eating contaminated shellfish or aquatic plants, and drinking contaminated water. Secondary transmission results instead from a 'person-to-person' infection. Thus, primary transmitted cases should be scattered in space, occurring almost simultaneously in different areas with no apparent interconnection, and should be located relatively close to water sources. These initial cases would be followed by the clustering of secondary transmitted cases . Miller and colleagues further proposed that primary transmission shapes the seasonal patterns in endemic areas, whereas secondary transmission determines the level of infection.
It is important to note that secondary transmission can occur directly or indirectly (i.e., via fecally contaminated water), and that it can involve the same aquatic reservoirs than those of primary transmission. These two modes of transmission effectively represent the two extremes of a possible continuum, with the key distinction being the degree of memory in the current force of infection of previous levels of infection. Only the secondary route possesses this memory, and this is the sense in which 'human-to-human' transmission should be interpreted.
The study of spatial cholera patterns by Craig further addressed the existence of clustering with case data from 1970 to 1982 at the level of baris, patrilineally related household groups . A significant excess of cases was detected at short distances, "within baris" of less than 250 meters, and short time intervals (3 and 10 days). However, this analysis did not take into account that the spatial location of cases is not random and that the distribution of the baris themselves must be taken into account. It also did not consider the timing between the occurrence of clusters and the epidemic peaks, and the nature of the clustering (positive or negative). Nevertheless, Craig's study was the first description of cholera's spatial distribution in an endemic area at high resolution.
We further examine here the spatio-temporal distribution of cholera cases in Matlab, Bangladesh, to address the occurrence of clustering during epidemic years. We specifically consider the relationships between cluster size and epidemic intensity, between the timing of clustering and the epidemic curve, and that between clusters of cases and water sources. Primary transmission from water reservoirs is believed to occur when environmental conditions are favorable [19, 24]. A spatial association between cholera cases and water sources should be observed if primary transmission drives the development of epidemics, with no significant spatial correlation between observed cholera cases at the beginning of the outbreak . Because water sources (rivers, canals and water ponds of diverse size) are found almost everywhere and are extremely abundant in our study area, they are not likely to impose significant spatial constraints on the occurrence of cases. Thus, cases generated by environmental transmission should occur almost anywhere. By contrast, once epidemics have reached a threshold size, secondary transmission should dominate the dynamics, generating spatial associations between cases. This pattern should be most pronounced during the fall peak after the monsoonal rains, when secondary transmission has been suggested to play a more important role [17, 21], since flooding disrupts sanitary conditions and concentrates human populations in the landscape. This hypothesis would predict a significant clustering of cases during the fall peak and a weak spatial association between cholera cases and water sources. We examine these predictions here.
People in this area live in groups of patrilineally related households known as bari, with an average of six households . Starting in 1994, a spatial database was created to facilitate the analysis of health and population research. All the baris were identified by a census number by the ICDDR, B (International Centre for Diarrhoeal Disease Research, Bangladesh) demographic surveillance system, allowing incidence data to be linked to the specific location of a single bari [25, 26]. Complementing the information on baris, geographic coordinates of major water sources (i.e., big water ponds) were also acquired. Untreated surface water from ponds and rivers is used by villagers for household uses, but there is conflicting evidence about its use for drinking purposes [26, 27], given that water from tube wells has been found to be contaminated with arsenic. Our analyses focus on the cholera cases from 1983 to 2004 for 8340 baris of this spatial database.
Health data were obtained from both surveillance and laboratory analyses. Individual health and demographic surveillance data were regularly collected for all individuals living in the Matlab study area. The Matlab hospital is the only diarrheal treatment center in the rural area, providing free treatment to all patients. Stool samples for all patients were collected and screened for enteric pathogens in the laboratory. Cholera cases were registered using the date of admission to the hospital and both biotype and serotype were reported. Thus, with approval of the ICDDR, B for this study we only used anonymous surveillance data (in agreement with the World Medical Association Medical Ethics Manual).
Initially, we studied the spatial distribution of cholera cases in order to detect the presence of spatial trends (i.e., first order trends, see Additional File 1). Although the distribution of cholera cases seems to display spatial trends (Additional File 2, Fig. S1), when this distribution is corrected by the spatial heterogeneity of the baris, such spatial trends are not statistically significant (Additional File 2, Fig. S2-S9). Some spatial trends have been previously reported  but, different spatial and temporal scales of aggregation for the data were considered in those studies. In addition, by performing Monte Carlo replications to evaluate the significance of the results (see details bellow), we explicitly considered the heterogeneity in the spatial distribution of the baris, an underlying pattern that was not taken into account in previous analyses.
where the function I h (d ij ) indicates whether or not the events i and j occurred within a distance less than or equal to h; and w ij is an edge correction to handle arbitrary shaped regions [29, 30]. It is important to notice that the K function considers the intensity, the mean number of events (infections) per unit area, of the process under analysis, and therefore the detection of a clustered distribution means that the number of cases (more properly, events) is above the expected density of cases at that particular scale.
When (h) is plotted against h, the peaks for positive values indicate spatial attraction of events, or clustering, whereas the troughs for negative values indicate the spatial repulsion, or regularity, at the corresponding scale of distance h in each case. Zero values indicate a random spatial distribution. The Ripley's K function provided in the R package "spatstat" was used for these analyses . Ripley's K and L functions were calculated by aggregating the daily cholera data into temporal windows ranging from 2 to 17 days. This temporal window represented potential infectious periods . In addition this also allowed the inclusion of sufficient cases to statistically evaluate clustering of cases for the different strains.
The spatial clustering and/or repulsion of cases-water was studied by using a cross K function. For this analysis, cholera cases and water sources are considered as two different types of events. Rivers and canals were not included in this analysis. Distance to main rivers have been proven to be unrelated with cholera cases in this area . Frequent dryness of canals make them a very unlikely source of disease.
Under the assumption of independence between types of events (cholera cases and water sources), the location of one type should be random with respect to that of the other type, regardless of the overall spatial distribution of either type. Hence if cases originated from water sources, the location of cases will be correlated with the location of water sources, and therefore the K function will identify such relationship as clustering at particular scales. Following the previous reasoning, the L function can be used to consider the interaction of two (or more) types of events, to evaluate spatial attraction, independence or repulsion (see  chapter 4 for details). An equivalent bootstrapping technique was used to evaluate the statistical significance of the clustering of cases-water.
For both spatial analyses (clustering of cases and cases-water), the characteristic cluster size was defined as the distance corresponding to the largest significant difference (i.e., the most significant difference). Hence, the largest difference between the observed L values and the corresponding superior envelope from the bootstrapping was considered as the characteristic cluster size . Note that during the course of an outbreak, significant clusters may occur at several scales (Fig. 3), but only the most significant is considered for the analyses presented in this work.
During the study period, several cholera outbreaks with different intensity occurred. To reduce the variability presented in the data we defined (and analyzed) epidemics as outbreaks that surpassed 30 new cases during the course of a week. This is based on the observed distribution of epidemic sizes. Although epidemics exhibited the typical seasonal pattern of two peaks per year during most of the study period in this endemic region, the timing of initiation of these peaks exhibited variability from year to year. Based on the ideas from Cazelles and Stone , we rescaled time for each epidemic as follows: Each epidemic was mapped onto the interval |0, 4π|, with the beginning mapped to time 0, the first (fall) peak to π, the inter-peak decay to 2π, the second (spring) peak to 3π and finally the end of the epidemic to 4π. Four stages (1 to 4) were thus defined, with stages 1 and 3 corresponding to the rise of epidemics (fall and spring), and stages 2 and 4, to their decay. Given that no biological cross-immunity between O1 and O139 strains has been reported , the dynamics of O139 epidemics can be considered independent from that of 01 and was therefore not considered here.
A less clear pattern is obtained for the clusters sizes corresponding to cases-water (Fig. 5b). Clusters of different sizes are present at different times, although few clusters seem to be present during the winter (corresponding to 2π in Fig. 5b).
Presence of significant clustering for cases.
Proportion of time with sig. clustering of cases
El Tor Inaba
Presence of significant clustering for cases-water.
Proportion of time with sig. clustering of cases-water
El Tor Inaba
Discussion and Conclusions
Our results indicate that primary transmission sparks the start of epidemics at several distant locations more or less synchronously. This generates the clustering of cases only at big scales with respect to water sources, as opposed to small clusters close to a few punctual sources. Secondary transmission follows, and the clustering of cases at relatively small scales becomes dominant. At the larger scales, however, these new cases create a relatively homogeneous spatial distribution because of the multiple origins of seasonal outbreaks, and therefore clustering with respect to water sources persists for those scales most of the time. The onset of epidemics is therefore controlled by both secondary and primary transmission. However, rapid increases in the number of cases are clearly tracked by spikes in clustering, indicating that secondary transmission plays a fundamental role in the development of epidemics. These findings are consistent with the interpretation of cholera patterns previously proposed by Miller and colleagues .
Additional support for the fundamental role of secondary transmission during the onset of the epidemics is provided by the fact that the "epicenter" of the epidemic (calculated as the center of mass for the location of cases) moves during the course of the outbreaks (results not shown). A more constant epicenter would reflect the spread of epidemics from an initial source of contamination.
Spatial clustering practically disappears during inter-epidemic periods, as expected, with a few randomly spatially distributed cases (if any) occurring until the beginning of another outbreak (Fig. 5 and Fig. 6). The spatial clustering of cases is detected at several scales, with two dominant sizes, given by hundreds of meters and a few kilometers, consistent across the different epidemics. These two scales suggest the occurrence of long distance transmission events that might be triggered (among other causes) by the movement of asymptomatic individuals, given their importance during epidemics .
The frequency of cluster occurrence (Tables 1 and 2) showed that the clustering of cases-water occurs more often than that of cases. This difference is consistent with the observation of cases-water clustering from the very beginning of outbreaks as primary transmission triggers epidemics. It is also consistent with the clustering of cases emerging with some delay but still in the early stages of epidemics, as secondary transmission becomes established and dominates the dynamics (Fig. 5 and Fig. 6). This pattern exhibits small differences among the different strains. The contamination by secondary cases of new water sources could explain the persistence of clustering at large scales for cases-water.
The spatial clustering pattern observed during the decay of the outbreaks is sometimes indistinguishable from a random spatial pattern (Fig. 5 and Fig. 6). This could be due to several factors. One possibility is that, the increase in rainfall and associated dilution effect, might eliminate the spatial structure. In addition, heterogeneity in the recovery time of infected individuals and behavioral changes, induced by the presence of cases, would alter the observed spatial pattern. This random pattern could also reflect a failure of the chain of transmission of the disease, with the re-initiation of epidemics from environmental sources.
The lack of strong seasonality on the presence of cases-water clustering (Table 2) could reflect the effectiveness of water sources as a reservoir and their role as a 'link' between the clusters of secondary cases. Some of the variability in the effectiveness of water sources might result from strong El Niño-Southern Oscillation (ENSO) events which have been shown to be strongly associated with cholera epidemics .
This work presents results on the spatial distribution of cholera cases during twenty-one years in Matlab, Bangladesh, that support the role of secondary transmission in the dynamics of the disease. During the study period a mass vaccination program took place in Matlab [35–37]; the cholera vaccine appears to provide temporal immunity and reports suggest that herd immunity was present at some spatial scales [38–40]. Our analyses of spatial trends shows, however, that for the scales considered in this study the effects of immunization were not important (see supplementary material). The spatial information from this vaccination program, together with more detailed local information (i.e., sanitary conditions, education and socioeconomic status at the level of baris or even individuals), could be incorporated in further studies that address the localization of the clusters and disease risk. In particular, local clustering methods may be useful for analyzing the effects introduced by the construction of a flood control embankment that protects the northern half of the study area in the late1980's [41, 42].
Finally, the analyses presented in this work are based on a static framework, where clustering is calculated from snapshots. A natural next step is to incorporate dynamical feedbacks between disease processes and the state of the population together with seasonality. Stochastic spatio-temporal models, similar to the ones developed for the advance of the "epidemic front" for rabies in the USA [2, 43], are under construction. The rabies model identified in particular the consequences of spatial heterogeneity for the spread of the front of infection. Endemic dynamics such as those of cholera in Bangladesh require the development of stochastic models that not only describe the propagation of a front but are also able to include the "back-propagation" to areas with both previously uninfected (still susceptible) and/or recovered individuals. Nested models can be formulated to evaluate the role of primary and secondary transmission, as well as the importance of immunity and cross-immunity in determining the complex spatial patterns observed in cholera dynamics [44, 45]. This work would extend the current scope of metapopulation and individual-based models of infectious diseases, to consider the stochastic dynamics that arise at high spatial resolution for diseases with temporary immunity. Such high resolution models would provide a basis to examine how to scale the dynamics up to the population level, to the simpler temporal models that are currently used for cholera and other infectious diseases in confronting time series data (e.g. [1, 2, 5, 20, 46–53]). These approaches will lead to a better understanding of the importance of spatial structure in the temporal dynamics of the disease.
This work was supported by the National Science Foundation-National Institutes of Health (Ecology of Infectious Diseases Grant EF 0430 120) and the National Oceanic and Atmospheric Administration (Oceans and Health Grant NA 040 AR 460019). M.P. is an investigator of the Howard Hughes Medical Institute.
The data used for this research study were collected with funding by the International Centre for Diarrheal Disease Research, Bangladesh (ICDDR, B) and its donors, who provide unrestricted support to the Centre for its operation and research. Current donors providing unrestricted support include: the Australian International Development Agency (AusAID), the Government of Bangladesh (GoB), the Canadian International Development Agency (CIDA), Embassy of the Kingdom of the Netherlands (EKN), the Swedish International Development Cooperative Agency (SIDA), and the Department for International Department, UK (DFID).
- Grenfell BT, Bjørnstad ON, Kappey J: Travelling waves and spatial hierarchies in measles epidemics. Nature. 2001, 414: 716-723. 10.1038/414716a.View ArticlePubMedGoogle Scholar
- Smith DL, Lucey B, Waller LA, Childs JE, Real LA: Predicting the spatial dynamics of rabies epidemics on heterogeneous landscapes. Proceedings of the National Academy of Sciences. 2002, 99: 3668-3672.View ArticleGoogle Scholar
- Jolles AE, Sullivan P, Alker AP, Harvell CD: Disease transmission of aspergillosis in sea fans: Inferring process from spatial pattern. Ecology. 2002, 83: 2373-2378. 10.1890/0012-9658(2002)083[2373:DTOAIS]2.0.CO;2.View ArticleGoogle Scholar
- Ferguson NM, Cummings DAT, Fraser C, Cajka JC, Cooley PC, Burke DS: Strategies for mitigating an influenza pandemic. Nature. 2006, 442: 448-452. 10.1038/nature04795.View ArticlePubMedGoogle Scholar
- Longini IM, Nizam A, Xu S, Ungchusak K, Hanshaoworakul W, Cummings DA, Halloran ME: Containing pandemic influenza at the source. Science. 2005, 309: 1083-1087. 10.1126/science.1115717.View ArticlePubMedGoogle Scholar
- Atti MLCD, Merler S, Rizzo C, Ajelli M, Massari M, Manfredi P, Furlanello C, Scalia Tomba G, Iannelli M: Mitigation measures for pandemic influenza in Italy: an individual based model considering different scenarios. PLoS ONE. 2008, 3: e1790-10.1371/journal.pone.0001790.View ArticleGoogle Scholar
- Snow J: On the mode of communication of cholera. 1855, London: John Churchill, New Burlington Street, England, 139-Google Scholar
- Miller CJ, Feachem RG, Drasar BS: Cholera Epidemiology in Developed and Developing Countries New Thoughts on Transmission, Seasonality, and Control. Lancet. 1985, 1: 261-263. 10.1016/S0140-6736(85)91036-0.View ArticlePubMedGoogle Scholar
- Mooi FR, Bik EM: The evolution of epidemic Vibrio cholerae strains. Trends In Microbiology. 1997, 5: 161-165. 10.1016/S0966-842X(96)10086-X.View ArticlePubMedGoogle Scholar
- Uma G, Chandrasekaran M, Takeda Y, Nair GB: Recent advances in cholera genetics. Current Science. 2003, 85: 1538-1545.Google Scholar
- Sack DA, Sack RB, Nair GB, Siddique AK: Cholera. The Lancet. 2004, 363: 223-233. 10.1016/S0140-6736(03)15328-7.View ArticleGoogle Scholar
- Kaper JB, Morris JG, Levine MM: Cholera. Clinical Microbiology Reviews. 1995, 8: 48-86.PubMedPubMed CentralGoogle Scholar
- Faruque SM, Sack DA, Sack RB, Colwell RR, Takeda Y, Nair GB: Emergence and evolution of Vibrio cholerae O139. Proceedings Of The National Academy Of Sciences USA. 2003, 100: 1304-1309. 10.1073/pnas.0337468100.View ArticleGoogle Scholar
- Reidl J, Klose KE: Vibrio cholerae and cholera: out of the water and into the host. Fems Microbiology Reviews. 2002, 26: 125-139. 10.1111/j.1574-6976.2002.tb00605.x.View ArticlePubMedGoogle Scholar
- Koelle K, Pascual M, Yunus M: Pathogen adaptation to seasonal forcing and climate change. Proc Biol Sci. 2005, 272: 971-977. 10.1098/rspb.2004.3043.View ArticlePubMedPubMed CentralGoogle Scholar
- Blokesch M, Schoolnik GK: Serogroup conversion of Vibrio cholerae in aquatic reservoirs. PLoS Pathogens. 2007, 3: e81-10.1371/journal.ppat.0030081.View ArticlePubMedPubMed CentralGoogle Scholar
- Pascual M, Bouma MJ, Dobson AP: Cholera and climate: revisiting the quantitative evidence. Microbes and Infection. 2002, 4: 237-245. 10.1016/S1286-4579(01)01533-7.View ArticlePubMedGoogle Scholar
- Faruque SM, Islam MJ, Ahmad QS, Faruque AS, Sack DA, Nair GB, Mekalanos JJ: Self-limiting nature of seasonal cholera epidemics: Role of host-mediated amplification of phage. Proceedings Of The National Academy Of Sciences USA. 2005, 102: 6119-6124. 10.1073/pnas.0502069102.View ArticleGoogle Scholar
- Ahmed MS, Raknuzzaman M, Akther H, Ahmed S: The role of Cyanobacteria Blooms in Cholera Epidemic in Bangladesh. Journal of Applied Sciences. 2007, 13: 1785-1789.Google Scholar
- King AA, Ionides EL, Pascual M, Bouma MJ: Inapparent infections and cholera dynamics. Nature. 2008, 454: 877-880. 10.1038/nature07084.View ArticlePubMedGoogle Scholar
- Ruiz-Moreno D, Pascual M, Bouma M, Dobson A, Cash B: Cholera seasonality in Madras (1901-1940): Dual role for rainfall in endemic and epidemic regions. EcoHealth. 2007, 4: 52-62. 10.1007/s10393-006-0079-8.View ArticleGoogle Scholar
- Glass RI, Becker S, Huq MI, Stoll BJ, Khan MU, Merson MH, Lee JV, Black RE: Endemic cholera in rural Bangladesh, 1966-1980. Am J Epidemiol. 1982, 116: 959-970.PubMedGoogle Scholar
- Craig M: Time-space clustering of Vibrio cholerae 01 in Matlab, Bangladesh, 1970-1982. Social science & medicine (1982). 1988, 26: 5-13.View ArticleGoogle Scholar
- Huq A, Sack RB, Colwell RR: Cholera and Global Ecosystems. Ecosystem change and public health: A global perspective. 2001, 327-352.Google Scholar
- Ali M, Emch M, Donnay JP, Yunus M, Sack RB: The spatial epidemiology of cholera in an endemic area of Bangladesh. Social Science & Medicine. 2002, 55: 1015-1024. 10.1016/S0277-9536(01)00230-1.View ArticleGoogle Scholar
- Emch M: Diarrheal disease risk in Matlab, Bangladesh. Social Science & Medicine. 1999, 49: 519-530. 10.1016/S0277-9536(99)00146-X.View ArticleGoogle Scholar
- Huq A, Sack RB, Nizam A, Longini IM, Nair GB, Ali A, Morris JG, Khan MN, Siddique AK, Yunus M, Albert MJ, Sack DA, Colwell RR: Critical factors influencing the occurrence of Vibrio cholerae in the environment of Bangladesh. Appl Environ Microbiol. 2005, 71: 4645-54. 10.1128/AEM.71.8.4645-4654.2005.View ArticlePubMedPubMed CentralGoogle Scholar
- Ali M, Emch M, Donnay JP, Yunus M, Sack RB: Identifying environmental risk factors for endemic cholera: a raster GIS approach. Health Place. 2002, 8: 201-210. 10.1016/S1353-8292(01)00043-0.View ArticlePubMedGoogle Scholar
- Bailey TC, Gratell AC: Interactive Spatial Data Analysis. 1985, Harlow: Prentice HallGoogle Scholar
- Yamada I, Rogerson PA: An empirical comparison of edge effect correction methods applied to K-function analysis. Geographical Analysis. 2003, 35: 97-109. 10.1353/geo.2003.0005.Google Scholar
- R: A Language and Environment for Statistical Computing: R: A Language and Environment for Statistical Computing. R Foundation for Statistical ComputingGoogle Scholar
- Cazelles B, Stone L: Detection of imperfect population synchrony in an uncertain world. Journal of Animal Ecology. 2003, 72: 953-968. 10.1046/j.1365-2656.2003.00763.x.View ArticleGoogle Scholar
- Albert MJ, Alam K, Ansaruzzaman M, Qadri F, Sack RB: Lack of Cross-Protection Against Diarrhea Due to Vibrio cholerae 0139 (Bengal Strain) after Oral Immunization of Rabbits with V. cholerae Ol Vaccine Strain CVD103-HgR. The Journal of Infectious Diseases. 1994, 169: 230-231.View ArticlePubMedGoogle Scholar
- Pascual M, Rodo X, Ellner SP, Colwell RR, Bouma MJ: Cholera dynamics and El Niño-Southern Oscillation. Science. 2000, 289: 1766-1769. 10.1126/science.289.5485.1766.View ArticlePubMedGoogle Scholar
- Clemens JD, Harris JR, Khan MR, Kay BA, Yunus M, Svennerholm AM, Sack DA, Chakraborty J, Stanton BF, Khan MU, Atkinson W, Holmgren J: Field trial of oral cholera vaccines in Banglasedh. The Lancet. 1986, 328: 124-127. 10.1016/S0140-6736(86)91944-6.View ArticleGoogle Scholar
- van Loon FPL, Clemens JD, Chakraborty J, Rao MR, Kay BA, Sack DA, Yunus M, Ali M, Svennerholm AM, Holmgren J: Field trial of inactivated oral cholera vaccines in Bangladesh: results from 5 years of follow-up. Vaccine. 1996, 14: 162-166. 10.1016/0264-410X(95)00122-H.View ArticlePubMedGoogle Scholar
- Clemens JD, Sack DA, Harris JR, Van Loon F, Chakraborty J, Ahmed F, Rao MR, Khan MR, Yunus M, Huda N: Field trial of oral cholera vaccines in Bangladesh: results from three-year follow-up. Lancet. 1990, 335: 270-3. 10.1016/0140-6736(90)90080-O.View ArticlePubMedGoogle Scholar
- Ali M, Emch M, von Seidlein L, Yunus M, Sack DA, Rao M, Holmgren J, Clemens JD: Herd immunity conferred by killed oral cholera vaccines in Bangladesh: a reanalysis. The Lancet. 2005, 366: 44-49. 10.1016/S0140-6736(05)66550-6.View ArticleGoogle Scholar
- Emch M, Ali M, Root ED, Yunus M: Spatial and environmental connectivity analysis in a cholera vaccine trial. Soc Sci Med. 2009, 68: 631-637. 10.1016/j.socscimed.2008.11.025.View ArticlePubMedPubMed CentralGoogle Scholar
- Ali M, Emch M, Yunus M, Clemens J: Modeling spatial heterogeneity of disease risk and evaluation of the impact of vaccination. Vaccine. 2009, 27: 3724-3729. 10.1016/j.vaccine.2009.03.085.View ArticlePubMedGoogle Scholar
- Carrel M, Emch M, Streatfield PK, Yunus M: Spatio-temporal clustering of cholera: The impact of flood control in Matlab, Bangladesh, 1983-2003. Health & Place. 2009, 15: 771-782. 10.1016/j.healthplace.2008.12.008.View ArticleGoogle Scholar
- Myaux JA, Ali M, Chakraborty J, de Francisco A: Flood control embankments contribute to the improvement of the health status of children in rural Bangladesh. Bull World Health Organ. 1997, 75: 533-9.PubMedPubMed CentralGoogle Scholar
- Russell CA, Smith DL, Childs JE, Real LA: Predictive Spatial Dynamics and Strategic Planning for Raccoon Rabies Emergence in Ohio. PLoS Biology. 2005, 3: e883-10.1371/journal.pbio.0030088.View ArticleGoogle Scholar
- Koelle K, Pascual M, Yunus M: Serotype cycles in cholera dynamics. Proc Biol Sci. 2006, 273: 2879-2886. 10.1098/rspb.2006.3668.View ArticlePubMedPubMed CentralGoogle Scholar
- Earn DJ, Levin SA, Rohani P: Coherence and conservation. Science. 2000, 290: 1360-364. 10.1126/science.290.5495.1360.View ArticlePubMedGoogle Scholar
- Bouma MJ, Pascual M: Seasonal and interannual cycles of endemic cholera in Bengal 1891-1940 in relation to climate and geography. Hydrobiologia. 2001, 460: 147-156. 10.1023/A:1013165215074.View ArticleGoogle Scholar
- Ferrari MJ, Grais RF, Bharti N, Conlan AJK, Bjørnstad ON, Wolfson LJ, Guerin PJ, Djibo A, Grenfell BT: The dynamics of measles in sub-Saharan Africa. Nature. 2008, 451: 679-684. 10.1038/nature06509.View ArticlePubMedGoogle Scholar
- Finkenstädt BF, Grenfell BT: Time series modelling of childhood diseases: a dynamical systems approach. Journal of the Royal Statistical Society: Series C (Applied Statistics). 2000, 49: 187-205. 10.1111/1467-9876.00187.View ArticleGoogle Scholar
- Grenfell BT, Harwood J: (Meta)population dynamics of infectious diseases. Trends In Ecology & Evolution. 1997, 12: 395-399. 10.1016/S0169-5347(97)01174-9.View ArticleGoogle Scholar
- Grenfell BT, Wilson K, Finkenstadt BF, Coulson TN, Murray S, Albon SD, Pemberton JM, Clutton-Brock TH, Crawley MJ: Noise and determinism in synchronized sheep dynamics. Nature. 1998, 394: 674-677. 10.1038/29291.View ArticleGoogle Scholar
- Ionides EL, Bretó C, King AA: Inference for nonlinear dynamical systems. Proceedings Of The National Academy Of Sciences USA. 2006, 103: 18438-18443. 10.1073/pnas.0603181103.View ArticleGoogle Scholar
- Rohani P, Earn DJ, Finkenstädt B, Grenfell BT: Population dynamic interference among childhood diseases. Proc Biol Sci. 1998, 265: 2033-2041. 10.1098/rspb.1998.0361.View ArticlePubMedPubMed CentralGoogle Scholar
- Rohani P, Earn DJ, Grenfell BT: Impact of immunisation on pertussis transmission in England and Wales. Lancet. 2000, 355: 285-286. 10.1016/S0140-6736(99)04482-7.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/10/51/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.