Fig. 5

(A) The genomic organisation of the structural genes of the T6SS gene clusters from the representative strain VFBJ01. Components encoded by the core genes are shown in various colours. (B) Western blot analysis of Hcp expression in seven Vibrio furnissii strains. RNA polymerase beta subunit (EF-Tu) was used as the loading control. Original images were included in Supplemental Fig. 1. (C) Quantification of Escherichia coli MG1655 survival after a T6SS attack by the indicated V. furnissii strains. Surviving prey cells (MG1655) were determined by serial dilution and plating on rifampin-containing LB agar plates. Representative images from three independent biological replicates are shown in B and C. (D) Quantification of haemolysin expression. Haemolysin assays showed these V. furnissii strains grew in large colonies with strong beta-haemolysis on blood agar. Haemolysin-positive control: V. cholerae N16961; haemolysin-negative control: E. coli MG1655