Fig. 6
From: Immunization with a peptide mimicking lipoteichoic acid induces memory B cells in BALB/c mice
Detectionof cytokines in splenocytes stimulated by heat-killed S. aureus. Afterone month of the last immunization, all mice were inoculated with heat-killed S.aureus for 7 days. Isolated splenocytes were stimulated with heat-killed S.aureus (2×104 CFU/well) for 24 h. The concentrationof IL-6 (A), IL-2 (B), and IFN-γ (C) wasmeasured by ELISA. (A) IL-6, MAP2-3 vs. MAPctrl: p = 0.0065; MAP2-3 vs. Blank: p < 0.001. (B) IL-2, MAP2-3 vs. MAPctrl: p = 0.017; MAP2-3vs. Blank: p = 0.003. (C) IFN-γ, MAP2-3 vs. MAPctrl: p <0.001;MAP2-3 vs. Blank: p < 0.001. n = 6-10 mice/group. p-values:* p < 0.05, ** p < 0.01, *** p < 0.001.