Fig. 8
LY294002 abolished the protective effects of MSCWnt5a on endothelial cells. a The representative scratch images of endothelial cells at 0 h and 24 h are presented. b Wound healing percentage of endothelial cells with different treatment was shown. c The tube formation assay was conducted to detect endothelial cell angiogenesis, and Calcein AM fluorescent dye was used to make the tubular structure more visible. d Quantitative analysis of vascular brunching number with different treatment was counted. e Annexin V-FITC/PI was used to detect endothelial cell apoptosis. f Quantitative analysis was conducted by summing up the percentages of early apoptotic cells (Q1-LR) and late apoptotic cells (Q1-UR). The proportion of apoptotic cells with various treatment was shown. Results are expressed as mean ± SD, n = 3. ****P < 0.0001 compared to LPS group. ####P < 0.0001 compared to MSCWnt5a group