Fig. 7
MSCWnt5a co-culture activates the PI3K/AKT signaling in endothelial cells. a The expression of AKT/p-AKT, VE-cadherin and apoptosis-related proteins (BCL-2 and BAX) were evaluated by Western blotting. b-e Densitometric analysis of Western blots. GAPDH served as an internal reference. f Detecting the effect of LY294002 on MSCWnt5a co-culture induced changes in the PI3K/AKT pathway via Western blotting. g-h Densitometric analysis of Western blots. GAPDH served as an internal reference. Results are expressed as mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 compared to LPS group