Fig. 6
Co-culturing with MSCWnt5a enhanced proliferation and reduced apoptosis of endothelial cells. a The EdU assay was conducted to measure the proliferation of endothelial cells. The blue color labeled nucleus and red labeled actively proliferating cells. b Quantitative analysis was performed by counting the ratio of actively proliferating cells. The proportion of proliferation-active cells with various treatment was shown. c The Annexin V-FITC/PI assay was performed to detect endothelial cell apoptosis. d Quantitative analysis was conducted by summing up the percentages of early apoptotic cells (Q1-LR) and late apoptotic cells (Q1-UR). The proportion of apoptotic cells with various treatment was shown. Results are expressed as mean ± SD, n = 3. ****P < 0.0001 compared to control group, ###P < 0.001, ####P < 0.0001 compared to LPS group